Labshake search
Citations for Beckman :
351 - 400 of 425 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Evolutionary Biology 2023Quote: ... viral cDNA was purified either by standard PCR reaction desalting columns or with a 0.45X volume of AMPure XP beads (Beckman Coulter). Nextera XT libraries were generated with 1 ng of cDNA material ...
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bioRxiv - Genomics 2023Quote: Sheared DNA was purified by binding to an equal volume of AMPure XP beads (A63881, Beckman Coulter, Inc. Brea, CA) and eluted in 50µl of 10mM Tris-HCl ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...
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bioRxiv - Immunology 2019Quote: ... SSM cultures were grown for ∼22 h at 36°C with shaking at 180 rpm until the OD600 reached 0.5 on a 1 cm path width spectrophotometer (Beckman Coulter DU 530). The cultures were then diluted to 1 × 109/ml with SSM.
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bioRxiv - Physiology 2020Quote: ... we computed [Hb] in a suspension of intact RBCs from the Hb absorbance measured on a UV/Vis spectrophotometer (model 730 Life Science, Beckman Coulter, Inc.), using an equation described previously22 ...
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bioRxiv - Cancer Biology 2022Quote: ... cell lysate or purified recombinant PKM2/PKL was added to start the assay and absorbance at 340 nm was recorded using a spectrophotometer (DU® 700, Beckman Coulter). To determine the knockdown efficiency of siRNA ...
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bioRxiv - Molecular Biology 2019Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 1x and 0.9x reaction volumes of Agencourt AMPure XP beads (Beckman Coulter - A63880). Purified libraries were combined as a 12 sample equimolar pool containing the indexes 1-12 and sequenced on an Illumina NextSeq on a single high-output flow cell (single-end 75 bp reads).
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bioRxiv - Molecular Biology 2019Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 1x and 0.9x reaction volumes of Agencourt AMPure XP beads (Beckman Coulter - A63880). Purified libraries were combined as an 8 sample equimolar pool containing the indexes 5-12 and sequenced on an Illumina NextSeq on a single high-output flow cell (paired-end 75 bp reads).
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bioRxiv - Molecular Biology 2021Quote: ... Cell suspension was supplemented with 500 μl of 0.1% w/v BSA/PBS for adjustment of the volume prior to the analysis and subjected to flowcytometric analysis using Cell Lab Quanta SC (Beckman Coulter).
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bioRxiv - Neuroscience 2021Quote: ... The reaction volume was increased to 50 μl and the tagmentated DNA was purified using 90 μl of Ampure XP beads (Beckman Coulter) and an elution volume of 40 μl of H2O ...
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bioRxiv - Molecular Biology 2020Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with a 1.5X reaction volume of AMPure XP beads (Beckman Coulter, Cat #A63882) and eluted in 13.5 μl TE buffer ...
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bioRxiv - Biochemistry 2020Quote: ... The reaction volume was adjusted with H2O to 40 μL and then purified with 72 μL AMPure XP beads (Beckman Coulter), eluting into 20 μL of H2O.
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bioRxiv - Cancer Biology 2020Quote: ... The pooled and normalized PCR reactions were purified using 1.8x the PCR reaction volume of AMPure XP beads (Beckman Coulter Inc). Samples were prepared for sequencing on Illumina MiSeq using the manufacturer’s standard library preparation protocol ...
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bioRxiv - Microbiology 2021Quote: ... The particle volume was then measured for a minimum of 100 000 particles per sample using a Multisizer 4e Coulter counter (Beckman Coulter) equipped with a 30 μm aperture tube (size range 0.6 μm to 18 μm ...
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bioRxiv - Microbiology 2022Quote: A 3 ml volume of pseudoparticles was pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... from the gradient were washed free of sucrose by dilution into 25 volumes of cold PBS (pH 7.4) and pelleted by ultracentrifugation at 150,000 × g (Beckman Ti 70 rotor) for 1 h at 4 °C ...
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bioRxiv - Plant Biology 2022Quote: ... The beads were removed from the sample with a magnetic rack and 28.8 µL (1.8X volume) of pre-warmed RNAClean XP beads (Beckman Coulter Life Sciences) was added then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Developmental Biology 2019Quote: ... with a final double (0.65 x −1 x bead volume) size selection step using a SPRIselect reagent kit (Beckman Coulter, B23318). The libraries were indexed (New England BioLabs ...
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bioRxiv - Microbiology 2020Quote: ... We pooled the triplicate PCR products and the 24 μL of final volume were purified by adding 44 μL of AMPure XP beads (Beckman Coulter), mixing with pipette followed by a 5 minute incubation ...
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bioRxiv - Immunology 2021Quote: ... Residual primers and dNTPs were again degraded enzymatically (ExoSAP-IT) and dsDNA was purified using 0.8 volumes of SPRI beads (SPRIselect, Beckman Coulter Genomics). 40uL of eluted dsDNA was amplified again with 10 pmol of each primer in a 100 μl total reaction volume (HotStarTaq Plus) ...
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bioRxiv - Genomics 2021Quote: ... The remaining 20 μL of each pool were pooled and clean-up was performed using an equal volume of AMPure XP beads (Beckman Coulter) and quantified using 1 μL of sample with the Qubit dsDNA HS Assay Kit on a Qubit 2.0 Fluorometer ...
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bioRxiv - Genomics 2022Quote: ... samples were then transferred to a new 96-well plate at a standard volume (typically 20-30 μL) using a BioMek FXp liquid handler (Beckman Coulter). This plate was diluted to a final TIME-Seq starting concentration of 10 ng/μL using the Mantis liquid handler.
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bioRxiv - Molecular Biology 2023Quote: ... Size selection purifications following the ligation and amplification PCR steps were performed with 0.9× reaction volumes of Agencourt AMPure XP beads (Beckman Coulter A63880). Purified libraries were combined as an 8-sample equimolar pool containing a combination of indexes from 1–12 and sequenced on an Illumina NextSeq on an Illumina NextSeq 2000 on a P2 flow cell (paired-end 75-bp reads).
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bioRxiv - Genomics 2022Quote: ... The sample collected from the BluePippin instrument was cleaned-up and concentrated using an equal volume of AMPure XP Beads (Beckman Coulter), with the DNA eluted in in 60 μL DNAse and RNAse free water ...
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bioRxiv - Genomics 2023Quote: ... After a subsequent wash step with ice cold 70 % ethanol the resulting DNA pellet was resuspended with 100 μL PCR-grade water followed by further purification via solid-phase reversible immobilization using 1.5 volume of AMPure XP Beads (Beckman Coultier™) and final elution in 20 μL 1× TE ...
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bioRxiv - Plant Biology 2023Quote: ... The reaction progress of degradation of nucleotides was analyzed by HPLC using 30 µM TTN5 in 30 µl injection volume (Beckman Gold HPLC, Beckman Coulter). After completion of the reaction ...
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bioRxiv - Molecular Biology 2023Quote: ... The content of the PCR tubes was pooled and mixed with an equal volume of AMPure XP beads (Beckman Coulter, A63881). After 5 min incubation ...
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bioRxiv - Cell Biology 2023Quote: ... RNA was extracted from the eluates using Trizol LS as described above and then further cleaned by using 1.8x volume of RNAClean XP beads (Beckman Coulter A63987) and washed in 80% ethanol.
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bioRxiv - Cell Biology 2023Quote: ... protein-liposome or protein-LD mixtures were combined with equal volume of 60% Optiprep solution (D1556-250ML, sigma) and placed at the bottom of the centrifuge tube (Beckman, 344090), and 10% Opti-prep and 0% Optiprep solutions were carefully pipetted on the top ...
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: ... 631 fragments at 100 mM concentration in DMSO were prepared in either Echo Qualified 384 low dead volume COC microplates (Beckman Coulter) or Echo Qualified 384 well polypropylene microplates (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... The circles of interest in the sample were then purified through the circle purification step detailed above (section 2.4.) using a 1.8x volume ratio of AMPure XP beads (Beckman Coulter, CA, USA). The efficiency of each sample purification was assessed through a standard qPCR assay using SYBR™ Green PCR Master Mix (ThermoFisher ...
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bioRxiv - Microbiology 2024Quote: ... The PCR products from thew first PCR were individually purified using 0.6X volumes of AMPure XP magnetic beads (AMPure XP beads kit / Beckman coulter, USA), followed by a second PCR reaction to attach the dual indices (S5 and N7 ...
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bioRxiv - Genomics 2020Quote: ... The quantity of RNA was analysed using spectrophotometer readings and normalized to 62.5ng/μl with a Biomek NXP (Beckman-Coulter, Mississauga, ON, Canada) automated liquid-handling instrument ...
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bioRxiv - Microbiology 2020Quote: ... samples absorbance at 485 nm was measured in a 1 cm quartz cuvette using a DUTM 730 spectrophotometer (Beckman Coulter, Brea, CA, USA). 155 ...
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bioRxiv - Biochemistry 2022Quote: ... The carboy was placed in an incubator (Sanyo MCO-17A1C) at 37 C and the optical density (OD) was monitored at 550 nm (Beckman DU-800 spectrophotometer) via 1 mL samples removed from the same port ...
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bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
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bioRxiv - Microbiology 2022Quote: Whole-cell absorbance spectra from 550 to 800 nm were taken from cells grown in BG-11 under VL and FRL for 30 days using a DU 640 spectrophotometer and associated software (Beckman Coulter, Brea, CA). For low-temperature fluorescence measurements at 77 K ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB medium containing 50 μg/mL Kanamycin at 37°C to an OD600~1.0 (as measured in a Beckman Coulter DU 640 spectrophotometer) and then quickly transferred on ice to cool for ~20 mins ...
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bioRxiv - Cell Biology 2023Quote: ... either the dye was solubilized with 10% (v/v) acetic acid and the absorbance was measured at 570 nm in a spectrophotometer microplate reader (Beckman Coulter, Model AD340), or alternatively ...
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bioRxiv - Microbiology 2024Quote: Cell growth was determined by measuring the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730, Beckman Coulter, Brea, CA, USA). The concentrations of the products (acetate and ethanol ...
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bioRxiv - Microbiology 2024Quote: Extracts were then diluted 20-fold (in 33% acetic acid) and their absorbance at A595 determined using a spectrophotometer (Beckman Coulter, DU-800).
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
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bioRxiv - Microbiology 2021Quote: ... Duplicate PCR products of the same sample were pooled in equal volumes and cleaned by 0.45x of Agencourt AMPure XP Magnetic Beads (Beckman Coulter, Pasadena, CA) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2022Quote: ... and then purified and concentrated using 0.5-fold volume of Agencourt AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA, Cat # A63881) yielding 12.2 µg of DNA (174.0 ng/µl) ...
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bioRxiv - Plant Biology 2022Quote: ... The cDNA was eluted from the beads with 25 µL RNase-free water and purified again with 25 µL (1.0X volume) of Ampure XP beads (Beckman Coulter Life Sciences) then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Genetics 2022Quote: ... The samples were size selected with 0.65x volume of Agencourt® AMPure® XP magnetic bead solution (Beckman Coulter, Brea, CA, USA), and suspended in 20µL elution buffer (Qiagen ...
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bioRxiv - Bioengineering 2019Quote: ... Equal volume of each barcoded product was pooled into amplicon libraries and purified using Agencourt AMPure XP system (Beckman Coulter, CA, USA) then examined on Agilent Bioanalyzer 2100 for product size distribution ...