Labshake search
Citations for Beckman :
301 - 350 of 425 citations for Small Volume Spectrophotometer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: Cell growth was measured based on the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730; Beckman Coulter). The fermentation products were assayed as previously reported (19) ...
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bioRxiv - Genomics 2019Quote: ... the three experimental replicates per sample were combined and purified using 1.8X volume of AmpureXP beads (Beckman and Coulter). After cleaning ...
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bioRxiv - Genetics 2020Quote: ... were further purified to remove unligated adapters using 0.9X volume AMPure XP beads as per the manufacturer’s protocol (Beckman-Coulter) eluting in 35μl EB.
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bioRxiv - Genomics 2021Quote: ... containing a mixture of DPBS and shearing master mix at a final volume of 2.14 μL using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter). The Astrios was equipped with a 100 μm nozzle at 25 psi ...
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bioRxiv - Cell Biology 2021Quote: ... Platelet number and volume were determined in whole blood and PRP samples using DxH 500 hematology analyzer (Beckman Coulter). Platelet count (mean ± SD ...
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bioRxiv - Plant Biology 2022Quote: ... The final PCR products were purified with 26.5 µL (1.0X volume) of Ampure XP beads (Beckman Coulter Life Sciences) then incubated at RT for 15 min then washed and dried as described above ...
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bioRxiv - Genomics 2020Quote: ... eluted in 6 μl TE and size-selected using a 0.9X volume of Ampure XP Beads (Beckman Coulter A63882) to remove excess oligos.
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bioRxiv - Molecular Biology 2019Quote: After depletion of the gene of interest samples were purified using a 1 X volume XP beads (Beckman Coulter), quantified and subsequently subjected to 75-bp-single-end sequencing on the Illumina Nextseq 500 platform.
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bioRxiv - Genomics 2021Quote: ... plates of 96 single cell libraries purified using a 1.2x volume of AMPure XP beads (Beckman Coulter, catalog # A63881). All libraries were then quantified using the Qubit dsDNA HS kit (Life Technologies) ...
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bioRxiv - Neuroscience 2020Quote: ... The cDNA from the PCR reaction was purified using 0.7x volume of SPRIselect beads (Beckman Coulter, cat. no. B23318). The quantity and quality of cDNA was measured using an Agilent Bioanalyzer hsDNA chip ...
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bioRxiv - Genomics 2020Quote: ... Sheared DNA was purified with Agencourt AMPure® XP Reagent (1.8x sample volume) (Beckman Coulter, Lane Cove, NSW, Australia). The quantity and size of sheared material was visualized on an Agilent Bioanalyzer DNA 7500 chip (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... Adaptor-ligated DNA was cleaned by adding 0.8 volumes of AMPure XP beads (Beckman Coulter Inc., Brea, CA, USA). Sequencing libraries were loaded on a SpotOn Flow Cell (FLO-MIN106 R9.4 ...
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bioRxiv - Immunology 2021Quote: ... Thereafter a cDNA clean-up was performed using 0.6x and 0.8x volumes of Agencourt AMPure XP SPRI beads (Beckman Coulter) to eliminate short fragments (less than 500 bp) ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... cell volume distributions and cell numbers per mL were determined using a Coulter Counter (Beckman Coulter, Z2 Particle Counter). From each culture ...
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bioRxiv - Cancer Biology 2023Quote: ... Post-PCR clean-up of the libraries was performed by adding 1.3× volume of Ampure XP beads (Beckman Counter) and incubated for 10 min at RT ...
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bioRxiv - Microbiology 2023Quote: ... DNA was size-selected by cleaning up with 0.45x volume of Ampure XP buffer (Beckman Coulter, Brea, CA, USA) and eluted in 60 μl EB buffer (Qiagen ...
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bioRxiv - Genomics 2023Quote: ... The depleted samples were then size selected using 0.6x volume of AMPure XP Beads (Beckman Coulter, Brea, CA, USA). Libraries were amplified with 10 and 6 PCR cycles for the 8-plex and 96-plex libraries ...
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bioRxiv - Immunology 2023Quote: ... Thereafter a cDNA cleanup was performed using 0.6x and 0.8x volumes of Agencourt AMPure XP SPRI beads (Beckman Coulter) which was then quantified using a Qubit dsDNA HS Assay Kit (Life Technologies) ...
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bioRxiv - Bioengineering 2023Quote: ... MB concentration and number- and volume-weighted size distributions were measured using a Multisizer 3 Coulter Counter (Beckman Coulter). MB concentration (𝑐i ...
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bioRxiv - Plant Biology 2023Quote: ... Granule size distribution was analysed and plotted in relative volume/diameter using the Multisizer 4e Coulter counter (Beckman Coulter) (SEP S4).
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bioRxiv - Cell Biology 2024Quote: ... A volume of 0.4 μL of ligand was added to cell plates using a BiomekFX Lab Automation Workstation (Beckman) and a previously calibrated pin tool ...
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bioRxiv - Cell Biology 2023Quote: ... Compounds were serially diluted in an Echo® Qualified 384-Well Low Dead Volume Source Microplate (0018544, Beckman Coulter) to generate a 21-point dose response at 1:3 dilution starting from a concentration of 10mM ...
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bioRxiv - Genomics 2023Quote: ... Post-PCR clean-up was performed without size selection using 1.3× volume of AMPure XP beads (Beckman Coulter, A63881).
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bioRxiv - Microbiology 2023Quote: ... Genomic DNA was further cleaned with 1.8 volumes of Ampure XP beads according to the manufacturer’s protocol (Beckman Coulter). RNA was isolated from Blastocystis cultures with a Trizol extraction following manufacturer’s protocols (Invitrogen).
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bioRxiv - Developmental Biology 2023Quote: ... Size selection and purification was achieved by double-sided (0.65 × −1× volume) using SPRIselect magnetic beads (Beckman Coulter, B23318). Concentrations were measured using the Qubit HS dsDNA assay ...
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bioRxiv - Developmental Biology 2024Quote: ... A two-size selection of fragments was performed using 0.5x and 1.3x volume of AMPure XP beads (Beckman Coulter). Libraries were quantified and analyzed using Qubit and Tapestation assays ...
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bioRxiv - Microbiology 2020Quote: ... Cultures of 1 ml in PYNal were incubated at 30°C (250 rpm) to an optical density of 0.2 at 620 nm (OD620; Beckman DU650 spectrophotometer) in 96-deep-well plates ...
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bioRxiv - Plant Biology 2020Quote: ... and then the supernate was used to determine SC and SP using an ultraviolet spectrophotometer (UV 530, Beckman Coulter, Brea, USA). The SC content was determined using the phenol-sulfuric acid method (Kochert ...
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bioRxiv - Cell Biology 2019Quote: ... and monitoring the optical density changes at 400 nm (ΔOD400) for 10 min with 10-sec intervals in a DU720 spectrophotometer (Beckman Coulter) at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The indigoidine concentration was determined by measuring the optical absorbance at 600 nm using a spectrophotometer (DU730, Beckman Coulter, Inc., USA).
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Genetics 2019Quote: ... We isolated RNA from 100,000 cells in RLT with 2.2X volume Agencourt RNAClean XP SPRI beads (Beckman Coulter, Danvers, MA). As measured by the RNA Qubit High Sensitivity Kit ...
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bioRxiv - Genomics 2019Quote: ... double stranded cDNA for each 96-well quadrant and used 0.8x volume of SPRI beads mix that contained 76.6 µl of the original RNAClean XP SPRI beads (Beckman Coulter Genomics) and 383 µl 2.5M Sodium Chloride ...
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bioRxiv - Genomics 2021Quote: ... Double-stranded DNA was purified from the second-strand reaction using 1.8x volume of AMPure XP SPRI beads (Beckman Coulter). As a negative control ...
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bioRxiv - Microbiology 2020Quote: ... water was added to a total volume of 50 μl and reactions were purified using AMPure XP beads (Beckman Coulter) with a 0.9X ratio according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... DNA fragmentation was performed on 10-ug DNA using 10 passes through a 22G needle in a 250-µL volume before purification using 0.5% Agencourt AMPure XP beads (A63882, Beckman Coulter). Each elution step of the AMPureXP beads was performed using 10 mM Tris-Cl pH 8.5 instead of water ...
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bioRxiv - Bioengineering 2020Quote: ... cDNA from each sample was generated with Smart-seq2 protocol using 20 µL total reaction volume and roughly 200 ng RNA input followed by 0.8X AMPure XP beads (Beckman Coulter) purification ...
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bioRxiv - Microbiology 2019Quote: ... including library amplification for 15 PCR cycles using custom indexed primers and post-PCR clean-up with 0.85x volume Ampure XP (Beckman Coulter). Libraries were quantified using Quant-iT PicoGreen dsDNA Assay Kit (Invitrogen ...
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bioRxiv - Synthetic Biology 2021Quote: Reactions were assembled in a final volume of 2 μL using a Labcyte Echo® 550 (Beckman Coulter, Brea, CA) via a two-step strategy ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries corresponding to samples to be directly compared to each other (e.g. DMSO vs UNC7040) were pooled together and purified using 1 volume of Agencourt AMPure XP (Beckman Coulter). The pooled libraries were eluted with 23 µL of elution buffer (NEXTflex ChIP-Seq kit ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with 1.5x reaction volume of AMPure XP beads (Beckman Coulter, A63881) and eluted in 13.5 μl TE buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... The reaction product (in the form of a cDNA:RNA hybrid) was purified with 1.5x reaction volume of AMPure XP beads (Beckman Coulter, A63881) and eluted in 16 μl TE buffer as described above ...
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bioRxiv - Genomics 2020Quote: ... the beads were removed from the solution using a magnet and DNA was purified using 1.8× volume of Agencourt AMPure XP (Beckman Coulter) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Twenty-five percent of the cDNA volume was subjected to fragmentation and double-sided SPRIselect (Beckman Coulter, Indianapolis, IN, USA) was used for PCR purification and clean-up ...
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bioRxiv - Molecular Biology 2022Quote: ... Ligase was deactivated using 5mM EDTA and heat at 65°C for 10 minutes (up to X individual barcoded RNA ligations were pooled) and subsequent purified using 1.8x volumes of RNAClean XP beads (Beckman Coulter). Ligated RNA was then reverse transcribed using random hexamer (5Pseq-RT ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Plant Biology 2023Quote: ... and all granules purified within this fraction was resuspended in known volumes of Isoton II electrolyte solution (Beckman Coulter, Indianapolis). The suspension was analysed in a Multisizer 4e Coulter counter (Beckman Coulter ...