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Citations for Beckman :
351 - 400 of 555 citations for Mouse Anti Human Papilloma virus types 16 18 716 F10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... by dounce homogenization (20 strokes).The lysate was clarified by centrifugation at 56,000 rpm in a Type 70 Ti rotor (Beckman Coulter) at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 60 min at 4°C (XPN-100 ultracentrifuge, Type 45 Ti rotor, Beckman, USA). The supernatant was filtered through a 0.45 μm filter (Pall ...
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bioRxiv - Neuroscience 2023Quote: Monomeric wild-type α-syn was ultracentrifuged at 90,000 RCF for 1 hour at 4 °C to remove any amorphous aggregates (Beckman Coulter Optima Max-XP with MLA-130 rotor) ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Cell Biology 2023Quote: ... The concentrated CFS was then ultracentrifuged at 100,000 ˗ g and 4 °C for 3 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Bioengineering 2024Quote: ... The sample tubes were then centrifuged at 347,000 × g and 20°C for 3 hours using a Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Cell Biology 2023Quote: ... IgG2a Mouse-PE (A09142, Beckman Coulter), IgG1κ Mouse-AF647 (565571 BD Biosciences) ...
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bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts were prepared by incubating cells with lysis buffer for 15 min on ice followed by centrifugation for 15 min at 16,162 rcf (Beckman Coulter, Microfuge® 16). Extract concentration was quantified by bicinchoninic acid assay (Pierce #23225 ...
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bioRxiv - Cancer Biology 2021Quote: ... previously centrifuged for 16 h at 120,000 g to remove bovine exosomes using 40.1Ti rotor (L-80XP; Beckman Coulter®, Brea, CA, USA).) ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Microbiology 2023Quote: ... The PCR products were then pooled for each of the 16S rRNA and fungal ITS1 regions after a purification/equalization process with the AMPureXP Kit (Beckman Coulter, Inc., Brea). Primer dimers ...
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bioRxiv - Immunology 2020Quote: ... while human B cells were CD38+/- (Beckman Coulter #6699531; 1µl per test) and CD19 (BD #557791 ...
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bioRxiv - Plant Biology 2022Quote: Protoplasts from wild type pCOBL9:GFP and cas-c1 pCOBL9:GFP transgenic plants were selected in a sorter MoFlo Astrios EQ (Beckman-Coulter), with a 70 μm nozzle at a rate of 5,000 to 10,000 events per second and a fluid pressure of 60 psi ...
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bioRxiv - Molecular Biology 2020Quote: ... samples were filled into 38 mL open-top polypropylene tubes and subjected to DUC using a SW Type 32 Ti swinging bucket rotor (Beckman Coulter) in an Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2021Quote: ... The cleared supernatant was subsequently centrifuged at 40,000 rpm for 70 min to pellet the EVs using the type 70 Ti rotor in an Optima XPN-80 ultracentrifuge (Beckman Coulter). The supernatant resulting from this high-speed centrifugation is the EV-free ACM ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... Archaella were precipitated from the supernatant at 142400 g using Beckman Coulter Opima XE-90 ultacentrifuge and the fixed-angle titanium rotor Type 45 Ti (Beckman Coulter). The tafi remained in the supernatant and could be precipitated either during many hours of high-speed centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... The UC step was an EV depletion step where the FBS was ultracentrifuged at 118,000 × g (Type 45 Ti rotor, 38,800 rpm, Beckman Coulter, Brea, CA) for 18 h at 4°C followed by a filtration step (0.22 μm) ...
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bioRxiv - Immunology 2024Quote: ... The gradient section containing assembled encapsulins was removed via aspiration and nanocontainers were isolated in a final ultracentrifugation step in a Type 70Ti rotor (Beckman Coulter) spinning at 68,000 rpm for 2 hours at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 2.5 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge and a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Plant Biology 2024Quote: ... The supernatant was separated and centrifuged for 3 h at 200,000 g at 6°C using an Optima XPN-90 ultracentrifuge with a Type 45 Ti rotor (Beckman Coulter, USA). The supernatant was discarded ...
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bioRxiv - Molecular Biology 2024Quote: Density gradient ultracentrifugation using a SW Type 55 Ti swinging bucket rotor in LM-70 ultracentrifuge (Beckman Coulter, Brea, CA, USA) was achieved using OptiPrepTM iodixanol solution (60% iodixanol ...
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bioRxiv - Immunology 2021Quote: ... The collected media were filtered with a 0.45-μm filter and ultracentrifuged at 21,000 rpm using a Type 45 Ti rotor (Beckman Coulter, Brea, CA, USA), and the pellets were resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Neuroscience 2023Quote: ... Collection of cells and media for AAV harvesting began 72 hours following transfection before iodixanol gradient ultracentrifugation purification using a Type 70 Ti Fixed-Angle Titanium Rotor (Beckman-Coulter, 337922). Titer was calculated using digital droplet PCR (ddPCR ...
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bioRxiv - Cell Biology 2024Quote: ... while the supernatant was further centrifuged at 118.000 × g (38,800 rpm) (Type Ti 45 fixed-angle rotor, Beckman Coulter, k-factor 178) for 2.5 hours at 4°C to isolate small EVs that were resuspended in PBS and stored at –80°C.
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bioRxiv - Molecular Biology 2024Quote: ... placed at the bottom of the tube and centrifuged at 5,000x g for 15 min at 16 °C in a swinging bucket rotor (Beckman Coulter, JS-5.3 or JS-7.5). The coverslips were recovered and fixed with pre-chilled methanol (–20 °C ...
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bioRxiv - Genomics 2019Quote: Human RNA libraries were individually cleaned using Agencourt AMPure XP beads (Beckman Coulter, #A63881) and a double-sided procedure (left 1×-right 0.6×) ...
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bioRxiv - Microbiology 2022Quote: ... human PBMC were incubated with monoclonal antibodies against CD19 (PE-Cy7; Beckman Coulter; IM3628), CD3 (PerCP ...
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bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
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bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
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bioRxiv - Genomics 2021Quote: ... Nuclei used to isolate each cell type were analyzed and sorted using a MoFlo Astrios EQ cell sorter (Beckman Coulter, Brea, CA, USA). Nuclei pellets were centrifuged for 10 min at 1500g and processed immediately for gDNA extraction using a QIAamp DNA micro kit (Qiagen ...
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bioRxiv - Immunology 2021Quote: Immune cell types in Cohort 1 were measured from whole blood by 10-color flow cytometry with Navios flow cytometer (Beckman Coulter, CA, USA). Staining and gating strategies were previously described in detail by Aguirre-Gamboa et al ...
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bioRxiv - Biochemistry 2020Quote: ... The sample was centrifuged at 4°C for 1 hour and 40 minutes at 182,753 x g (38,000 rpm in a Beckman Coulter Type 42.2 Ti rotor).
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... supernatants were centrifuged at 100 000 x g for 70 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman, Brea, CA, USA) to pellet the EVs ...
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bioRxiv - Bioengineering 2023Quote: ... The diluted sample was then centrifuged using a Beckman ultracentrifuge (Optima L90K) with two cycles of 1h10 at 110,000 x g at 4°C (rotor type 70Ti, Beckman Floor Ultracentrifuge Optima L90K). Between them ...
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bioRxiv - Cell Biology 2024Quote: ... and centrifuged at 16,500 × g (14,500 rpm) at 4°C for 20 min using a Type Ti 45 fixed-angle rotor (Beckman Coulter, k-factor 1.275) to isolate large EVs ...
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bioRxiv - Immunology 2023Quote: ... for mouse samples or a CytoFLEX LX (Beckman Coulter) for human samples ...
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bioRxiv - Microbiology 2023Quote: ... briefly mixed and equilibrated for ∼30 min (Albumax equilibration was conducted in a 5% CO2 incubator to maintain pH 7.4) after which they were subjected to ultracentrifugation at 37°C using a sealed rotor (Beckman Rotor type 42.2 Ti; 223,000 x g) for 4.2 h to separate proteins ...
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bioRxiv - Microbiology 2020Quote: ... fluorescein isothiocyanate (FITC)-conjugated mouse monoclonal antibody KC57 (Beckman Coulter) was used at a dilution of 1:100.
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bioRxiv - Cancer Biology 2021Quote: ... The concentration of the expanding human T cells was calculated on a Coulter Multisizer (Beckman Coulter, Brea, CA) and maintained at 1.0-2.0 × 106 cells per mL in R10 media (RPMI-1640 plus GlutaMAX-1 ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT products were subsequently amplified using human BCR primers and cleaned up through SPRIselect beads (Beckman Coulter). Next ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Immunology 2020Quote: ... and with Cyto-stat tetrachrome (FITC anti-CD45, PECy5 anti-CD3, PE anti-CD4, and ECD anti-CD8, Beckman Coulter, catalog number 660713). Unstimulated cells and pokeweed mitogen-stimulated cells were additionally stained with AF750 anti-CD19 (Beckman Coulter ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD45RA (Beckman), anti-CD3 (BD Bioscience) ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...