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Citations for Beckman :
251 - 300 of 555 citations for Mouse Anti Human Papilloma virus types 16 18 716 F10 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The samples were centrifuged at 34,000 rpm at 4°C for 16 h using an SW60 Ti rotor (Beckman Coulter). Subsequently ...
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bioRxiv - Genetics 2021Quote: ... The sheared DNA fragments were then purified for each of the 16 mice strains using AMPure XP Beads (Beckman, USA), following the instructions of the reagent supplier.
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bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
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bioRxiv - Developmental Biology 2020Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in SW41 for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were dialyzed into NaLow and then were loaded onto a 5-20% sucrose gradient made in NaLow buffer and centrifuged at 35,000 rpm for 16 h in a SW41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 ml fractions ...
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bioRxiv - Developmental Biology 2022Quote: ... Samples were loaded onto 10-45% CHX supplemented sucrose gradients in 9/16 x 3.5 PA tubes (Beckman Coulter, #331372) and spun at 35,000 x g in an SW41 rotor for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100; Centrifuge: Beckman Optima TLX). 100ul from the top of the tube was collected as VLDL fraction ...
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bioRxiv - Cell Biology 2023Quote: ... down to 6 ml and ultracentrifuged at 10,000xg for 16 min at 4°C in MLA-80 rotor (Beckman Coulter). The pellet was resuspended and washed with PBS at the same conditions (volume ...
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bioRxiv - Biophysics 2023Quote: ... The isolated crude membrane extract was loaded on the continuous 20-70 % sucrose density gradient in 20 mM NaH2PO4/Na2HPO4 pH 7.4 and 100 mM NaCl prepared by the Gradient Station (Biocomp) and centrifuged for 16 h at 30.000 rpm (rotor SW 40 Ti, Beckman Coulter). The gradients were collected with the Gradient Station ...
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bioRxiv - Microbiology 2023Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Microbiology 2024Quote: ... The resulting gradient was then subjected to centrifugation for 16 hours at 150,000 x g and 4 °C (SW55 Ti rotor, Beckman-Coulter). Sample banding was visually observed at the interface of the 30% and 20% fractions (Supporting Information ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Cancer Biology 2024Quote: ... Each ATAC library was amplified with Nextera primers for 16 PCR cycles and purified with Agencourt AMPure XP (Beckman Coulter) to remove excess primers ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... The lysate was cleared by centrifugation (60 minutes, 40,000 RPM) in a Type 45 Ti rotor (Beckman Coulter), filtered and loaded onto a HisTrap HP column (GE Healthcare) ...
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bioRxiv - Molecular Biology 2022Quote: ... The solution was centrifuged for 1 h at 4°C and 45,000 rpm (Type 45 Ti rotor, Beckman) and the supernatant was bound to 2 mL (CV ...
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bioRxiv - Biophysics 2019Quote: ... and the lysate was clarified by centrifugation for 1 h at 192,838 g in a Type 50.2 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were spun for 70 minutes at 200,000 × g and 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Plant Biology 2022Quote: ... 330,000 x g / 60,000 RPM for 4.5 hours using a TY 70.1Ti rotor (Type 70.1 Ti Rotor, Beckman Coulter, USA) loaded into an Optima XE-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Neuroscience 2023Quote: ... Nuclei for the cell type of origin were sorted either on a MoFlo Astrio EQ sorter (Beckman Coulter), BD FACSAria II (Becton-Dickinson) ...
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bioRxiv - Biochemistry 2023Quote: ... Membrane fractions were isolated by ultracentrifugation at 40,000 x g in a type 45 Ti rotor (Beckman Coulter). Membrane fractions were further homogenised and washed twice with high-salt buffer (10 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Biochemistry 2019Quote: ... Gradients were centrifuged at 49,123 x g for 16 hours at 4°C using an SW32Ti rotor (Beckman Coulter, ref. 369694). Gradients were fractionated with a Brandel gradient fractionation system using a 1 mm UV cell detector ...
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bioRxiv - Plant Biology 2019Quote: ... for 16 h before the radiolabel was quantified by scintillation for 2 min using a LS6500 Multi-Purpose Scintillation Counter (Beckman Coulter). CPM values were scaled to the average for the 8 week long day plant sample at 6-9 mm or 8 week long day total samples.
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bioRxiv - Plant Biology 2022Quote: ... extracts were loaded carefully into thick-walled polycarbonate tubes with three-piece caps (10.4 mL, Polycarbonate Bottle with Cap Assembly, 16 × 76 mm - 6Pk, 355603, Beckman Coulter, USA) pre-filled with 2.5 mL sucrose cushion (SC ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...
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bioRxiv - Cancer Biology 2023Quote: ... Crosslinks were reversed with 25 µg Proteinase K for 16 hours at 65°C and DNA was purified using AmpureXP beads (Beckman Coulter). Enrichment was confirmed using qRT-PCR with known positive and negative binding sites for each protein target ...
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bioRxiv - Biophysics 2023Quote: ... The bottom-loaded 45-10% gradient was then centrifuged at 120 000 x gav for 16 h in a SW40 Ti Swinging Bucket rotor (Beckman Coulter). After ultracentrifugation ...
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Molecular Biology 2024Quote: ... the fixed samples were subjected to sucrose cushion centrifugation (7,000x g for 20 min at 16 °C) using a swinging bucket rotor (Beckman, JS-7.5). Coverslips were recovered ...
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bioRxiv - Cell Biology 2024Quote: ... Samples were then spun at 100,000 xg for 16 h at 4°C using a TLS-55 swinging-bucket rotor and benchtop ultracentrifuge (Optima Max-XP, Beckman Coulter). Fractions were collected and loaded in SDS-PAGE for WB.
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Molecular Biology 2020Quote: ... followed by ultracentrifugation at 200000 g for 120 minutes (Type 70 Ti rotor, Optima L-80 XP, Beckman Coulter). The pellet was washed in PBS and collected by ultracentrifugation at 200000 g for 120 minutes [17] ...
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bioRxiv - Cell Biology 2022Quote: ... Sonicate was supplied with 0.5 mM PMSF and clarified by centrifugation at 40,000 rcf for 60 min in a Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 15 ml Ni-NTA agarose (Qiagen ...
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bioRxiv - Microbiology 2019Quote: ... Conditioned media was then centrifuged at 36,000 × g for 3 hours at 4ºC (Type 45 Ti rotor, Beckman Coulter). OMV-containing pellets were resuspended in 2 mL ASW.
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 2 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 66,000 x g for 30 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 1.5 mL of anti-Flag M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2019Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 100,000 × g for 190 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman) to pellet the EVs (Fig ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifuging at 183,960 x g for 88 min in Type 70 Ti rotor (Beckman). The clarified supernatant was incubated with 4 mL of IgG Sepharose 6 Fast Flow beads (GE Healthcare Life Sciences ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were lysed via sonication (Branson Digital Sonifier) and clarified via centrifugation at 66,000 x g for 30 min in a Type 70 Ti rotor (Beckman) at 4°C ...
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bioRxiv - Plant Biology 2020Quote: ... 50 μg/ml cycloheximide) and centrifuged for 3 h at 55,000 g and 4 °C in a Type 70 Ti rotor (Beckman). The pellet was dissolved in 1% SDS ...
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bioRxiv - Cell Biology 2021Quote: ... by three passes through an EmulsiFlex-C5 (Avestin, Inc.) and then clarified by centrifugation at 40,000 rpm in a Type 45ti rotor (Beckman) for 1.5 hours at 4°C ...