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Citations for Beckman :
701 - 750 of 767 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Microbiology 2022Quote: ... and 25% (wt/wt in 12.5ml) sucrose gradient in 25 mM citrate buffer in 38 ml Ultraclear tubes (Beckman-Coulter, 344058). The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Plant Biology 2023Quote: ... the vesicle containing fraction was dispersed in 350 µl fractions in 1.5 mL (9.5 x 38 mm) in 6 polypropylene microfuge tubes (Beckman Coulter, Cat. # 357448) and the volume in each tube was brought up to 1,500 µl using 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Systems Biology 2023Quote: ... 5 mL of fluid was used for EV isolation using a SW55 Ti swinging bucket rotor (Rmin 61, Rmax 109, Beckman Coulter). To keep the k-factor consistent at each centrifugation step and taking into account the time needed for the rotor to achieve its desired speed (approximately 5 min) ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Biochemistry 2024Quote: ... Growth was monitored by sub-culturing cells repeatedly to 106 cells/ml and measuring densities 24 hours later using a Coulter Counter Z2 particle counter (Beckman Coulter). Transgenic trypanosomes were generated by standard procedures [38] ...
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bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell suspensions were diluted 250 μL into 10 mL of ISOTON diluent and cell size was measured on a Coulter Z2 Cell Analyzer (Cat# 844 80 11, Beckman, Indianapolis, IN). Median cell volume was used for calculations ...
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bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
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bioRxiv - Cell Biology 2021Quote: ... 100 µg/mL CHX and cOmplete™ EDTA-free Protease Inhibitor Cocktail) prepared in a polypropylene centrifuge tube (14 × 95 mm; Beckman Coulter). A Gradient Master (Biocomp ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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bioRxiv - Biochemistry 2020Quote: ... was layered on NaBr δ=1.28 g/mL and centrifuged at 207,000g for 22 h at 10 °C on a Beckman L8M centrifuge (Beckman, Palo Alto, CA). A tube layered with 20 mM Tris-HCl in lieu of the sample was used as a blank for density calculations ...
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bioRxiv - Biophysics 2021Quote: ... The density gradient was prepared by layering 4.5 mL of 30.4 % urografin solution on top of 4.5 mL of 53.2 % urografin solution in an ultra-clear centrifuge tube (Beckman Coulter; catalogue number 344059). The tubes were left at room temperature (25 °C ...
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bioRxiv - Microbiology 2019Quote: The 5-10 mL bioaerosol samples were concentrated by spinning at 3000 × g for 10 min (Allegra® X-15R, Beckman Coulter). The pellet was resuspended in 200 µl fresh 7H9 and stained overnight (12-16 h) ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The gradient was formed by sequentially adding each 3 ml layer to a 14 × 89 mm ultra-clear centrifuge tube (Beckman Coulter, Inc.), starting with the 40% solution and ending with the 5% solution ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cell Biology 2019Quote: ... Following centrifugation at 35,000 rpm for 24 hr at 4°C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Neuroscience 2019Quote: ... iodixanol solution and overlaid with 7.0 mL of 25% (v/v) iodixanol and 350 µL of 5% (v/v) iodixanol in a polyalomar tube (Beckman, Miami, FL). All of the above iodixanol solutions were prepared in lipid flotation buffer (10 mM HEPES ...
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bioRxiv - Neuroscience 2021Quote: ... 100 mL of each well in the 8-well strip was purified with 60 mL Ampure XP beads (0.6x ratio; Beckman Coulter, Brea, CA), washed twice with 75% ethanol ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Genetics 2022Quote: ... Four μL from each successful reaction were combined into one 1.5 ml microfuge tube and the primers were removed using Agencourt AMPure™ XP beads (Beckman Coulter #A63881).
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bioRxiv - Molecular Biology 2023Quote: The 50-mL cultures were centrifuged in a Beckman-Coulter Allegra 25R temperature-controlled benchtop centrifuge (Beckman Coulter Inc, Brea, CA, USA) at 4°C and 6,000 RCF for 15 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... and a 200-μl aliquot of the final supernatant was added to 3 mL of scintillation fluid and the radioactivity was measured by a liquid scintillation counter (Beckman LS 6000SC). Blank values were obtained by performing the reaction in the absence of tissue homogenate and in the presence of boiled homogenate ...
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bioRxiv - Neuroscience 2022Quote: ... was loaded onto a sucrose gradient made up of 5 ml 1.2 M sucrose on the bottom and 5 ml of 0.8 M sucrose at the top in thin wall ultracentrifugation tubes (Beckman Coulter, Cat # 344059). The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Bird weight and red blood cell count (RBC: number of red blood cells per ml of blood, Beckman Coulter Counter, Series Z1) were measured immediately before the infection and every two days thereafter ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was again centrifuged at 100000 g for 45 min at 4°C (Beckman Optima™ MAX-XP Rotor MLS-50). After filtering the supernatant through a 0.22 μm syringe filter ...
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bioRxiv - Plant Biology 2023Quote: ... The combined cAW and vAW solutions were then centrifuged using 29 x 104 mm (50 mL) polypropylene centrifuge tubes (Beckman Coulter, # 357007) at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Cell Biology 2023Quote: ... 150 μL of the recovered sample was mixed with 150 μL of 60% Optiprep and layered with 200 μL of 10% Optiprep and 150 μL of the reconstitution buffer in a 0.8 mL tube (Beckman coulter Cat. #344090). The tube was centrifuged at 40,000rpm for 90 minutes in a SW-55 rotor ...
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bioRxiv - Neuroscience 2023Quote: ... 150 µl of 80% Nycodenz (in the same buffer) was mixed with the sample and then loaded into a 0.8 ml tube (Beckman Coultier, catalog #344090), overlaid with 250 µl of 30% Nycodenz ...
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bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 mM DTT and 0.1 mM ATP) to a final volume of 50 μL in 1.5 mL polypropylene centrifuge tubes (Beckman Coulter, Brea, CA, USA) suited for high–speed centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... Following centrifugation at 131,300 × g for 24 hr at 4 °C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Bioengineering 2023Quote: ... PFs obtained from 6 ml of plasma (24 ml total volume as previously described) were concentrated to 6 ml and added to a Beckman Coulter ultra-clear centrifuge tube (344059, Beckman Coulter, USA). A density gradient was carefully constructed by underlying 2 ml each of 10% ...
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bioRxiv - Plant Biology 2024Quote: ... Two mL of the remaining supernatant was loaded on 12% and 33.5% sucrose layers cushion in 13.2 mL tubes (Ultra-Clear, Beckman Coulter, United States, 344059) and centrifuge in a Beckman L-100K class S ultracentrifuge (W40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2024Quote: ... cultures were maintained at 1-2*106 cells/mL in semi-chemostat by daily dilution with fresh medium based on cell counts (Multisizer 4e coulter counter, BECKMAN COULTER, USA).
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bioRxiv - Bioengineering 2024Quote: ... PCR reactions were pooled into eight 1.5 mL Eppendorf tubes (600 uL) with an equal volume of SPRI (Beckman-Coulter, Brea, CA) beads that incubated for 5 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
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bioRxiv - Bioengineering 2021Quote: ... 10 µl of which was added to 10 ml of ISOTON® diluent and counted on the COULTER COUNTER® Z1 Series Particle Counter (Beckman Coulter). From this count ...
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bioRxiv - Microbiology 2019Quote: ... and the remaining 32 mL were centrifuged at 500 × g for 3 min at 4°C (Allegra X-15R, Beckman Coulter, Villepinte, France) to spin down the remaining meat fibers and debris ...
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bioRxiv - Biophysics 2020Quote: ... Cells were grown in LB medium containing 50 μg/mL Kanamycin at 37°C to an OD600~1.0 (as measured in a Beckman Coulter DU 640 spectrophotometer) and then quickly transferred on ice to cool for ~20 mins ...
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bioRxiv - Immunology 2021Quote: ... 39 mL of cGAMP-VLP-containing supernatant was gently overlaid on 6 mL of cold PBS containing 20% sterile filtered endotoxin free sucrose in 6 Ultra-Clear tubes (Beckman Coulter, ref 344058), and centrifuged for 1 hour and 30 minutes at 100,000 g 4°C ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...