Labshake search
Citations for Beckman :
501 - 550 of 767 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Developmental Biology 2023Quote: ... Sorting was performed using a MoFlo MLS high-speed sorting flow cytometer (Beckman Coulter), BD FACS Aria II or Fusion running FACS Diva 4.1 (BD Biosciences) ...
-
bioRxiv - Biochemistry 2023Quote: ... before being transferred into 32.4 mL Optiseal Polypropylene Tubes (Beckman Coulter, Brea, CA, USA). Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor ...
-
bioRxiv - Neuroscience 2023Quote: ... 1.4 ml eluate were collected and liposomes sedimented in a TLA-55 rotor (Beckman) at 35.000 rpm for 2 h at 4°C ...
-
bioRxiv - Biochemistry 2024Quote: ... 0.1 mM ATP) in 1.5 mL polypropylene centrifuge tubes (Beckman Coulter, Brea, CA, USA) suited for high–speed centrifugation to final concentrations ranging from 0.75 µM – 10 µM ...
-
bioRxiv - Bioengineering 2024Quote: ... The supernatant was then added slowly to a 14-mL polystyrene ultracentrifuge tube (Beckman) containing 1.5 mL of 30% sucrose (Thermo ...
-
bioRxiv - Cell Biology 2023Quote: ... in PBS in a 38.5 ml thin-wall ultra-clear ultracentrifuge tube (Beckman Coulter) and ultracentrifuged using SW32 Ti rotor in Optima XE-90 ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Cell Biology 2023Quote: ... in PBS inside a 32 mL open-top thickwall polycarbonate ultracentrifuge tube (Beckman Coulter) and centrifuged using SW32 Ti rotor in Optima XE-90 ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Molecular Biology 2024Quote: ... The supernatant was transferred to ultracentrifuge tube (38.5 ml-Beckman Coulter Ultra-Clear™) for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter) ...
-
bioRxiv - Microbiology 2024Quote: ... The mix was loaded into 5.2 ml QuickSeal Polyallomer tubes (Beckman Coulter Pasadena, USA) and isopycnic density ultracentrifugation was carried out at 44,100 rpm for 36 hours at 20°C in an Optima XPN-80 ultracentrifuge (Beckam Coulter ...
-
bioRxiv - Molecular Biology 2019Quote: ... the two initial washes were performed with PBS containing 0.5% Triton x-100 and cells were stained for 30 min with an α-p24 (gag) antibody (Coulter Clone KC57-RD1; Beckman Coulter). Data were acquired with a BD FACSVerse flow cytometer and analyzed using the FlowJo package (FlowJo LLC ...
-
bioRxiv - Cancer Biology 2020Quote: ... CM was then sequentially ultra-centrifuged at 100,000 x g at 4 ° C for 2 hrs in a Beckman Coulter Optima L-100 XP Ultracentrifuge using an SW41-Ti rotor and polycarbonate centrifugation tubes (Beckman Coulter; #331372). The EV pellet ...
-
bioRxiv - Microbiology 2021Quote: ... Vesicles were collected from cell-free supernatant culture by ultracentrifugation at 100000 × g (centrifuge Optima XPN-100 Beckman Coulter and rotor SW28) at 4 °C for 1.5 hours ...
-
bioRxiv - Microbiology 2021Quote: ... cells were diluted 100-fold in PBS and green fluorescence (488 nm) and were measured using a cytoFLEX S (Beckman Coulter, USA). Data were analyzed using CytExpert (Beckman Coulter ...
-
Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
-
bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
-
bioRxiv - Biochemistry 2022Quote: ... 000 g at 4°C for 30 min in a Beckman Coulter Optima L- 100 XP ultracentrifuge using 14 mm × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Ultra-pure mitochondria were harvested at the interphase between 19% and 40% and washed three times with 1xM buffer ...
-
bioRxiv - Microbiology 2020Quote: ... We pooled 1.5 µl of each indexed library (including blanks and swabs) and performed size selection for fragments approximately 100-500 bp in length with AMPure XP beads (Beckman Coulter, IN). The final pooled library was quantified with a Qubit High Sensitivity fluorometer and the fragment length distribution evaluated with the 2200 TapeStation system ...
-
bioRxiv - Microbiology 2021Quote: ... supernatant containing pseudovirions was harvested by centrifugation at 2,000 rpm for 10 min and concentrated by Optima XPN-100 Ultracentrifuge (Beckman Coulter, California, USA). For pseudovirus infection ...
-
Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
-
bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
-
bioRxiv - Bioengineering 2024Quote: Exosomes from each cell line were isolated from a conditioned medium based on differential ultracentrifugation (Optima™ XE-100, Beckman Coulter, USA) [57] ...
-
bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
-
bioRxiv - Cell Biology 2020Quote: ... 40 μL of fixed cells were diluted in 10 mL diluent (Isoton II; Beckman Coulter) and sonicated for 5 pulses of approximately 0.5 second each at low power ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclease treated media was next transferred to 38-mL Ultra-Clear centrifuge tubes (Beckman Coulter) and centrifuged for 60 min at 133,000 x g at 4°C in a Sure Spin 630 36 mL rotor (Thermo Scientific) ...
-
bioRxiv - Molecular Biology 2021Quote: ... and layered onto the bottom of a 38-mL Ultra-Clear centrifuge tube (Beckman Coulter). Lower-density solutions were prepared by diluting homogenization buffer (250 mM sucrose ...
-
bioRxiv - Bioengineering 2022Quote: ... samples (10 mL) were first centrifuged at 7000Xg at 4°C for 30 minutes (Beckman Coulter Avanti J-E Series ...
-
Enterohepatic Transcription Factor CREB3L3 Protects Atherosclerosis via SREBP Competitive InhibitionbioRxiv - Physiology 2020Quote: VLDL (d < 1.006 g/ml) was isolated via ultracentrifugation with a TLA120.2 rotor (Beckman Coulter). VLDL fractions were separated by sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE ...
-
bioRxiv - Bioengineering 2019Quote: ... Bacteria was concentrated to 1 mL by centrifugation (J2-MC, Beckman Instruments, Fullerton, United States) and stored in cryovials at −80°C (refrigerator type ...
-
bioRxiv - Molecular Biology 2022Quote: ... sonicated and then diluted in 10-20 ml of Isoton II diluent (Beckman Coulter #8546719) before measurement.
-
bioRxiv - Neuroscience 2022Quote: ... and 50% sucrose solutions (1.5 ml each) were layered into a centrifuge tube (Beckman, 331372). Brain total lysate (6 mg of protein ...
-
bioRxiv - Immunology 2022Quote: ... followed by centrifugation for 16 h at 40,000 rpm (MLS-50 swinging-bucket rotor, Beckman). Fractions with target protein of 1 ml were manually collected and concentrated to 200 µl by using Amicon Ultra-0.5 centrifugal filter unit with 100 kDa cutoff (EMD Millipore ...
-
bioRxiv - Biochemistry 2023Quote: ... 1.4 mL lysate was transferred to a tube and centrifuged (Beckman Coulter Microfuge 22R Centrifuge) at 18,000g for 10 min at 4°C ...
-
bioRxiv - Microbiology 2023Quote: ... 50%) in a 17 mL ultracentrifuge tube (Ultra-Clear, 16×102 mm, 344061, Beckman Coulter), and leaving overnight to allow for merging of the layers into a continuous gradient ...
-
bioRxiv - Cell Biology 2023Quote: ... The step gradient was formed by bottom loading 13 mL UltraClear tubes (Beckman Coulter, #344059) with a long metal cannula with 800 μL 2.5% ...
-
bioRxiv - Microbiology 2023Quote: ... pH 7.4) and loaded into a centrifuge tube (Beckman Cat. No. 344057, 5 ml capacity). One milliliter of 20% sucrose in 20 mM HEPES pH 7.4 ...
-
bioRxiv - Biophysics 2021Quote: ... 1ml acceptor LUV was diluted with 10 mM tris 100 mM NaCl and centrifuged to remove CsCl or sucrose (188,965 g, 37,500 rpm, 45 min, Beckman L8-80M, SW-60 rotor). The acceptor LUV pellet was resuspended in 1ml 20 mM tris 100 mM NaCl pH 7.4 buffer ...
-
bioRxiv - Biophysics 2022Quote: ... shortly sonified and centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1).) ...
-
bioRxiv - Biophysics 2022Quote: ... The samples were centrifuged for 30 min at 100 000 g at 4°C (Centrifuge Beckman Coulter Optima XPN-80, Rotor type 70.1). The supernatant ...
-
bioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 10,000xg for 30 minutes at 4°C in a Beckman Coulter Optima XE-100 (fitted with a SW41Ti Rotor (Beckman Coulter, CA USA)) ...
-
bioRxiv - Cell Biology 2021Quote: ... Supernatant was discarded and cells were resuspended in 100 μL FACs buffer and sorted with a CyAn™ ADP Analyser (Beckman Coulter, Inc.) at the UCL GOSICH Flow Cytometry Core Facility ...
-
bioRxiv - Immunology 2022Quote: ... followed by ultracentrifugation at 110,000 × g for 90 min at 4°C on an Optima XPN -100 ultracentrifuge with SW32Ti rotor (Beckman Coulter, Brea, CA, USA). The isolated EVs was washed with PBS and followed with a secondary ultracentrifugation (110,000 × g for 90 min at 4°C) ...
-
bioRxiv - Genomics 2019Quote: ... 300 ng of high molecular weight genomic DNA was fragmented to 100 – 700bp (300bp average size) with a Covaris S2 instrument (LGC Genomics) and cleaned up with Agencourt AMPure XP (Beckman Coulter, Brea, USA). DNA library preparation was performed using the NEBNext Ultra II DNA Library Prep Kit (New England Biolabs ...
-
bioRxiv - Cell Biology 2019Quote: ... The library fragments with a size range of 100-200 bp were selected with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA). Templates were prepared on the Ion Chef system using an Ion PI Hi-Q Chef kit (Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: Lipoprotein fractions were separated via sequential ultracentrifugation after progressively increasing the solvent density (29) using the ultracentrifuge model Optima XPN-100 (Beckman Coulter, California, USA) and a Kontron 45.6 fixed-angle rotor at 37000 rpm and 4 °C for different time intervals ...
-
bioRxiv - Immunology 2021Quote: ... following by staining for 15 minutes at room temperature with a 1:100 dilution of a fluorescent anti-HIV-1 GAG antibody (KC57-FITC, Beckman Coulter reference 6604665) in permeabilization buffer ...
-
bioRxiv - Cell Biology 2023Quote: ... supernatants were centrifuged at 100 000 x g for 70 min in ultracentrifugation using type 70 Ti rotor (Oprima XL-80K, Beckman, Brea, CA, USA) to pellet the EVs ...
-
bioRxiv - Microbiology 2023Quote: ... 2G12 was washed out with PBS twice followed by permeabilization with 0.2% Triton X-100 and staining for intracellular Gag with KC57-FITC (Beckman Coulter, Pasadena, CA; 6604665) diluted 1:100 in Dako antibody diluent ...
-
bioRxiv - Cell Biology 2023Quote: ... the resulting G-actin mix was ultracentrifuged at 90K rpm for 30 minutes at 4°C in an ultracentrifuge (Beckman, Optima TL 100) to remove actin oligomers and/or aggregates.
-
bioRxiv - Molecular Biology 2024Quote: ... Differential ultracentrifugation was performed by a SW 32 Ti swinging bucket rotor in an Optima XPN-100 ultracentrifuge (Beckman Coulter, Brea, CA, USA). The cell-conditioned serum-free media ...
-
bioRxiv - Molecular Biology 2024Quote: ... Cells with the highest GFP expression (top 20%) were isolated on a Moflo XDP 100 cell sorter (Beckman Coulter Inc., Brea, CA, USA) and expanded ...