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Citations for Beckman :
251 - 300 of 2122 citations for Human G Protein Coupled Receptor Family C Group 6 Member A GPRC6A ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... The membrane fraction was pelleted down by high-speed centrifugation (100,000 x g; 4°C; 30 min; 45Ti rotor; Optima XPN-80, Beckman Coulter). The membrane pellet was resuspended in solubilization buffer ...
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bioRxiv - Molecular Biology 2021Quote: ... Ultra-centrifugation was performed (160,000 x g; 16 hours; 4°C; SW 32 Ti rotor; Optima XPN-80, Beckman Coulter) and 1mL fractions were collected and analyzed by BN-PAGE ...
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bioRxiv - Microbiology 2021Quote: ... Concentrated supernatants were then subjected to ultracentrifugation at 160,000 x g for 2 hours at 4°C using a Beckman Ti 45 rotor (Beckman, Germany). Supernatants were completely removed and the pellet containing the crude MV fraction was resuspended in 400 μL of chilled PBS ...
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bioRxiv - Neuroscience 2022Quote: ... The gradient was subjected to high-speed centrifugation at 100,000 x g for 2 h at 4° C using a SW41Ti rotor (Beckman Coulter). 12 fractions of 1ml each were collected from the top of the gradient ...
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bioRxiv - Microbiology 2022Quote: ... was added before ultracentrifugation at 20,000 × g for 2 hours at 10°C in an Optima L-90K Ultracentrifuge with the SW32Ti rotor (Beckman Coulter). Supernatants were carefully discarded by aspiration and the 6 phage pellets were resuspended with 0.1 ml of TM buffer (50 mM Tris-HCl ...
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bioRxiv - Molecular Biology 2019Quote: ... followed by ultracentrifugation at 100,000 g for 2h in polyallomer tubes at 4 °C in a SW40 rotor (Beckman Coulter). Pelleted material was washed two times in filtered PBS at 100,000 g for 2h and re-suspended in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... and spun down at 4° C for 10 minutes at 12096×g using an Avanti J-25 centrifuge (Beckman Coulter). The sonicated lysate was incubated for 2 hours at 4 ° C with beads (Glutathione High Capacity Magnetic Agarose Beads ...
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bioRxiv - Biochemistry 2019Quote: ... The supernatant from 2,000 × g was transferred to 30mL conical tube and then centrifuged at 10,000 × g for 10 min at 4°C (# Avanti J-E JA25-50 Beckman Coulter). The supernatant from this spin was filtered through a 0.22-μm mesh filter (# SLGP033RS EMD Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... The V and VI fractions were then diluted separately to a total volume of 12mL with dfPBS and centrifuged at 100,000 × g for 70 min at 4°C (# Optima-XE SW41 Beckman Coulter). The final pellet from each fraction was resuspended in 30 μL of dfPBS ...
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bioRxiv - Synthetic Biology 2019Quote: ... then centrifuged at 5000 x g and 10°C for 10 minutes (Avanti□ J-E Centrifuge, Beckman Coulter, Indianapolis, IN). After disposing the supernatant ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 4°C for 3 h at 100,000 g by using Avanti J-30XP (Beckman, USA), and the fractions were collected using a Brandel Fractionation System (USA ...
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bioRxiv - Microbiology 2021Quote: ... The treated cells were pelleted by centrifugation at 12,000×g at 4 °C for 20 min (Beckman Allegra X-15R) and the supernatant was discarded ...
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bioRxiv - Molecular Biology 2021Quote: ... cell lysates were cleared by centrifugation at 4 °C for 5 minutes at 16000 x g and loaded onto ice-cold 15-50 % sucrose-CHX gradients and ultracentrifuged at 4 °C for 2 hours at 274 000 x g in a SW-41Ti rotor (Beckman). Gradients were fractionated and monitored at an absorbance of 254 nm with a fraction collector (BioComp Instruments ...
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bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
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bioRxiv - Neuroscience 2019Quote: ... The supernatant was layered onto a 20% sucrose cushion prepared in the same lysis buffer and then centrifuged at 186,000 x g for 2 h at 4 °C in a TLA-55 rotor ultracentrifuge (Beckman Coulter). RNA was isolated from the ribosome-enriched pellet using TRIzol LS reagent according to manufacturer’s instructions (Invitrogen).
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bioRxiv - Biochemistry 2019Quote: ... Ribosomes were pelleted via centrifugation at 63,000 x g for 16-18 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosome pellets were dissolved in resuspension buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Biochemistry 2019Quote: ... Fractions containing 40S and 60S ribosomal subunits as identified via absorption at 254 nM were pelleted via centrifugation at 63,000 x g for 20 hours at 4°C using a Type 80 Ti rotor (Beckman Coulter). Ribosomal subunits were resuspended in storage buffer (30 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: ... The homogenate was centrifuged for 10 min at 10,000×g at 4°C in a Ti-70 rotor and Optima XPN-90 ultracentrifuge (Beckman Coulter) and the supernatant was removed ...
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bioRxiv - Molecular Biology 2020Quote: ... by centrifuging at 63,000 x g for 18 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). Pellets were resuspended and these samples were analysed by mass spectrometry.
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bioRxiv - Physiology 2021Quote: ... the supernatant was ultracentrifuged at 100,000 g for 70 min at 4°C using an MLA-55 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... Samples were ultracentrifuged at 120,000 × g for 90 minutes at 4 °C in an Optima XPN-80 ultracentrifuge (Beckman Coulter) using a SW55 Ti swinging-bucket rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2021Quote: ... and cellular debris was removed by centrifugation at 90 000 x g for 20 min at 4°C (45 Ti rotor, Beckman). Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor ...
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Cell Biology 2020Quote: ... Tubes were centrifuged twice at 100,000 x g for 70 min at 4°C with a Beckman ultracentrifuge (32Ti rotor from Beckman Coulter). Supernatants were removed and the pellets resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 × g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep™ Genomic DNA Miniprep Kit (Axygen ...
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bioRxiv - Microbiology 2020Quote: ... Two distinct fractions were collected from the first CsCl density gradient centrifugation (17,000 × g, 60 min, 4°C in a fixed angle MLA-55 rotor, Beckman Coulter), each were sedimented (4000 × g ...
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bioRxiv - Microbiology 2021Quote: ... The resting supernatants were filtered through 0.45µm syringe filters and ultracentrifuged at 100,000 x g for 1h at 4°C (SW41 Ti swinging-bucket rotor, Beckman Coulter). The supernatant was discarded and the pellet suspended in 0.22 µm-pore filtered or 0.02 µm-pore filtered (for Flow Cytometry analysis ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Microbiology 2022Quote: ... clarified using 0.2 µm filter and pelleted at 54,000 rpm (180,000 ×g) for 2 hours at 4 °C in a TLA-55 rotor in an Optima Max-XP tabletop ultracentrifuge (Beckman). The supernatant was removed ...
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bioRxiv - Cell Biology 2022Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with a 18G needle ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Microbiology 2022Quote: ... The sample-containing tube was spun at 347,000 × g and 20°C for 3 hours using Type 70 Ti rotor (Beckman Coulter). 1-mL fractions were collected from the top of the tube ...
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bioRxiv - Microbiology 2022Quote: ... 20 mL of the filtrate was then ultracentrifuged at 150,000 × g at 4°C for 3 h (Beckman Coulter, USA) through a 20% (w/v ...
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bioRxiv - Neuroscience 2023Quote: ... followed by ultracentrifugation at 100,000 x g for 60 minutes at 4°C (SW 41 Ti Rotor, Beckman Coulter Inc). For size-exclusion chromatography (SEC ...
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bioRxiv - Immunology 2022Quote: ... eVLPs were purified by ultracentrifugation at 50,000 rpm (135,000 x g) for 2 hours at 4°C using a TLA100.3 rotor and a OptimaTM TLX ultracentrifuge (Beckman Coulter) on a 20% w/v sucrose cushion ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenised mixture then underwent ultracentrifugation at 200,000 g for 110 min at 4°C with a SW-41 Ti rotor (Beckman Coulter) and transferred the supernatant into a Slide-A-Lyzer™ G2 Dialysis Cassettes 2K MWCO (Thermo ...
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bioRxiv - Molecular Biology 2023Quote: 8 mL of viral supernatant on a 3 mL of 20% sucrose cushion were ultracentrifugated for 3 h at 100,000 x g at 4°C using an Optima L80-XP instrument (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...
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bioRxiv - Cell Biology 2022Quote: ... 4–40% sucrose gradient prepared in lysis buffer and centrifuged at 150,000 × g for 4 or 16 h at 4°C in a MLS-50 rotor (Beckman Coulter). Thereafter ...
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bioRxiv - Cell Biology 2023Quote: ... The iodixanol density gradients were centrifuged at 100,000 x g at 4◦C for 18 hours in SW41Ti rotor (Beckman Coulter). Ten fractions (F1 ...
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bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... then pelleted at 4°C and 4,300 x g for 30 min in an Allegra X-14R centrifuge (Beckman Coulter) equipped with a swinging bucket SX4750 rotor ...
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bioRxiv - Microbiology 2023Quote: ... Each tube was centrifuged for 3 hours at 150’000 x g (4°C) using an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW 32 Ti swinging bucket (369650 ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Cell Biology 2023Quote: ... The obtained supernatant was layered onto 30 w/v% sucrose solution in ultracentrifugation tubes and subjected to ultracentrifugation at 100,000 × g for 3 h at 4°C using SW32Ti rotor (Beckman courter). The collected sucrose solutions were mixed with PBS and washed by ultracentrifugation at 100,000 × g for 1 h ...
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bioRxiv - Systems Biology 2023Quote: ... The urine was centrifuged at 20,000 x g for 30 minutes at 4°C (k-factor 1,790) in an Optima XPN-80 ultracentrifuge (Beckman Coulter) equipped with a SW32 Ti swinging bucket rotor (Rmin 67 ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...