Labshake search
Citations for Beckman :
251 - 300 of 1939 citations for 6 Methyl 2 3 4 9 tetrahydro 1H carbazole 1 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2023Quote: ... Peptide supernatant was cleared by ultracentrifugation at 100,000□g for 1 hour at 4□°C (Beckman Coulter, 343775), then vacuum-dried overnight.
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 16.2500) and then bound to 0.1 cell paste volumes of anti-FLAG resin (Sigma M2 ...
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bioRxiv - Biochemistry 2019Quote: ... 4 °C (Beckman JLA 8.1000), washed in pre-chilled PBS and resuspended in 20 cell paste volumes of preparation buffer (50 mM Tris pH 8 ...
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bioRxiv - Biochemistry 2020Quote: ... A Multisizer 4 (Beckman Coulter) machine was used ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (SW55Ti rotor, Beckman). The pellets were resuspended in 25% (w/v ...
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bioRxiv - Cell Biology 2020Quote: ... 4°C (VTi65.1 rotor, Beckman) with the maximum acceleration and minimum deceleration allowing for isopycnic separation of subcellular particles and membranes ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C (SW41 rotor, Beckman). The supernatant was concentrated with a 100-kDa molecular weight cutoff concentrator (Merck Millipore ...
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bioRxiv - Biochemistry 2019Quote: ... SS-34 rotor) at 10,000 rpm at 4°C for 1 hours followed by 1 hour of ultracentrifugation (Beckman Optima™ LE, 80K, SW28 rotor) at 28,000 rpm (104000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
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bioRxiv - Microbiology 2023Quote: ... Three 1:2 serial dilutions were done suing a Biomek i7 liquid handler (Beckman Coulter, USA), in order to obtain the four working concentrations ...
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bioRxiv - Molecular Biology 2020Quote: ... diluted in 9 mL of 10 mM Tris-HCl buffer and centrifuged for 45 min at 45.000 rpm (Beckman 70.1 Ti Rotor). Pellets were suspended in the same buffer and analyzed by SDS-PAGE using 12.5% polyacrylamide gel ...
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bioRxiv - Cell Biology 2023Quote: ... EV–containing fractions were pooled (1.10–1.18 g/mL) and diluted by adding 9 ml PBS to SW40 tubes (Beckman–Coulter, Fullerton, CA, USA). EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Molecular Biology 2019Quote: ... The 80S fraction was pelleted for 1 h at 436000 x g at 4 °C (TLA-100, Beckman Coulter), resuspended in 20 mM HEPES pH 7.4 ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4 °C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Biochemistry 2022Quote: ... The crude extract was centrifuged at 200,000 g for 1 h at 4 °C (Beckman Coulter Optima LE-80K). The membrane pellet was homogenized and solubilized in 20 mM HEPES (pH 7.8 ...
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bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... Reaction mixtures were incubated for 30 minutes and centrifuged at 100,000g at 4°C for 1 hr using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Biochemistry 2019Quote: ... and centrifuged at 287,582 x g (90,000 rpm) for 1 hr at 4°C in a TLA100.2 rotor (Beckman, ref. 362046) in 11×34 mm thick-wall polycarbonate ultracentrifuge tubes (Beckman ...
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bioRxiv - Microbiology 2022Quote: ... The post-nuclear supernatant was adjusted to 0.8 M sucrose and loaded in the middle of a step gradient ranging from 0.1 to 1.8 M sucrose in STKM buffer and centrifuged at 50’000 g for 1 h at 4°C in a TLA-120.1 fixed-angle rotor (Beckman Coulter). LDs formed a white cushion on top of the tube and were collected by aspiration of the upper fraction of the gradient (125 µl).
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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Comparative membrane proteomics reveals diverse cell regulators concentrated at the nuclear envelopebioRxiv - Cell Biology 2023Quote: ... The gradients then were centrifuged at 35,000 rpm (210,000g) for 1 h at 4°C with no brake in an SW41Ti rotor (Beckman Coulter). Two samples were collected from the nucleus gradient ...
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bioRxiv - Neuroscience 2023Quote: ... the supernatant was collected and applied for further centrifugation at 40,000 rpm for 1 h at 4°C (45 Ti fixed-angle rotor, Beckman) to pellet the membranes ...
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bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 30 min at 4 °C and centrifuged at 100,000 × g at 4 °C for 1 h using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... After purification of nucleic acids by Agencourt RNA Clean XP (Beckman Coulter) and subsequent treatment with DNase I ...
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bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... performed ultracentrifugation (40,000 rpm, either 4 h or 18 h, +4 °C, SW41 rotor, Beckman Coulter), and collected approximately 600 μl fractions by puncturing the tubes with a thin (23G or 25G ...
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bioRxiv - Immunology 2022Quote: ... Flow cytometry was performed using a FACSort or FACScalibur flow cytometer (Becton Dickinson, Mountain View, CA) and a CyAN ADP LX 9-colour flow cytometer (Beckman Coulter, Pasadena, CA). Data were analyzed using FlowJo software (Tree Star Inc ...
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bioRxiv - Cancer Biology 2021Quote: ... 4°C (Beckman Coulter Allegra X15r). After the final wash to remove Matrigel ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C (SW41Ti rotor, Beckman Coulter) and 16 fractions were collected ...
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bioRxiv - Molecular Biology 2021Quote: ... 4°C (SS34 Rotor, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... 4°C (with Beckman SW32 rotor). The pellet will contain rough microsomes and the interface between 0.25-1.3M will contain smooth microsomes ...
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bioRxiv - Neuroscience 2021Quote: ... 4°C (Beckman Coulter F301.5 rotor) to remove large aggregates ...
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bioRxiv - Biophysics 2023Quote: ... 4 °C (TLA-45 Beckman rotor). The solubilized proteins were separated on 4–15 % BN-gels and analyzed by immune detection using polyclonal α-YidC antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...