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Citations for Beckman :
401 - 450 of 1603 citations for tert Butyl trans 17 bromo 4 7 10 13 tetraoxa 15 heptadecenoate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... Tubes were capped then centrifuged for 15 minutes at 30,000xg in a JLA 16.250 fixed angle rotor (Beckman-Coulter, Brea, CA) at 4°C using a Beckman Avanti JE centrifuge ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Microbiology 2021Quote: ... starting with 50% and topping off with 15% for a total of 11 mL was added to thin wall polypropylene ultracentrifuge tubes (Beckman Coulter). The tubes were immediately frozen and stored at -80 °C ...
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bioRxiv - Cell Biology 2020Quote: ... The first PCR reaction was performed for 15 cycles with 60 °C annealing and 30 s elongation and then purified with AMPure XP PCR beads (Beckman Coulter). The second PCR was performed for 15 cycles for wild type-specific and for 21 cycles for tag-specific amplicons respectively using 60°C annealing and 30 s elongation ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction mixtures were incubated at room temperature for 15 min followed by centrifugation at 36000 x g using Optima TL benchtop ultracentrifuge (Beckman Coulter) for 15 min at 4°C ...
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bioRxiv - Biochemistry 2022Quote: ... sucrose gradient and the RCII complexes were separated by ultracentrifugation (40,000×g,15 h) using an Optima XPN-90 Ultracentrifuge (Beckman Coulter, USA). The separated RCII/PSI fraction was collected and then washed repeatedly using the glycerol- and DM-free elution buffer and concentrated to 0.2 mg Chl/ml which corresponded to approximately 0.6 mg protein/ml.
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bioRxiv - Cell Biology 2022Quote: ... Thromboplastins and Fibrins the plasma was centrifuge at 4700Xg for 15 mins followed by high-speed centrifugation (Beckman Avanti J-26XP) at 12,000 x g for 25mins and filtered using a 0.22μm filter (Ultident Scientific ...
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bioRxiv - Molecular Biology 2020Quote: ... The target library derived from the oocyte RNA polymerase II transcripts was PCR-amplified (15 cycles for P14 WT, 16 cycles for P14 Tbpl2−/− mutant) and purified using AMPure beads (Beckman Coulter) to remove short PCR artifacts (< 200bp ...
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Genomics 2022Quote: ... was added to each reaction and incubated for 15 minutes at 37 °C followed by 15 minutes at 80 °C and the samples were purified using 1x AMPure XP beads (Beckman Coulter). For the Panhandle protocol ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Molecular Biology 2024Quote: ... and 1U of FastAP (TFS, EF0654) for 15 min at 37 °C and purified using 1.2× SPRIselect beads (Beckman Coulter, B23318). The inDrops-v1.1 libraries were digested with 40U of ExoI and 1U of FastAP for 15 min at 37 °C ...
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bioRxiv - Genomics 2024Quote: ... c) increasing PCR cycles (15 in total) and eliminating primer dimers prior to sequencing (Agencourt AMPure XP magnetic beads, Beckman Coulter). Primary PDAC were sequenced on the NovaSeq6000 ...
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Cell Biology 2023Quote: ... A volume of lysate containing 125 ug RNA was loaded on 11 ml 15-50% sucrose gradients in 14 x 89 mm centrifuge tubes (Beckman Coulter). (Gradients were prepared in 20 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum was obtained by centrifuging the tubes for 15 min (1000g, 22°C) (Allegra X-14R Centrifuge, Beckman Coulter, Brea, CA) and stored at -80°C for subsequent analysis ...
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bioRxiv - Physiology 2024Quote: ... The fibrils were precipitated by ultracentrifugation for 1.5 hours at 15°C in an Optima MAX-TL (Beckman Coulter, Fullerton, CA) with a TLA-120.2 rotor at 80,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... aureus grown overnight were harvested via centrifugation at 4000 rpm for 15 min (Microfuge 20R; Beckman Coulter, La Brea, CA, USA), the pellet was washed twice in phosphate-buffered saline (PBS ...
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bioRxiv - Cell Biology 2020Quote: ... The sample was centrifuged at 14,000 g for 10 min at 10°C and then loaded in an Optima analytical centrifuge (Beckman) in an An-50Ti rotor pre-equilibrated at 20°C ...
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bioRxiv - Microbiology 2021Quote: ... liquid-captured bioaerosols (5 – 10 mL) were centrifuged for 10 minutes at 3000 × g (Allegra X-15R, Beckman Coulter) and resuspended in 200 μL of Middlebrook 7H9 medium supplemented with 100 μM DMN-tre ...
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bioRxiv - Neuroscience 2021Quote: ... The gradient was centrifuged at 50,000xg for 21min at 4°C (Beckman Coulter Optima MAX XP ultracentrifuge with a TL-55 rotor) ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C for 30 min using a JLA 9.1000 centrifuge rotor (Beckman). E ...
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bioRxiv - Biochemistry 2020Quote: ... At OD600 1.2 the cells were pelleted at 4 °C (TLA10.500 (Beckman), 15 min at 5,000-8,000 rcf) ...
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bioRxiv - Immunology 2021Quote: ... Samples in Figure 4 were collected with a CytoFlex cytometer (Beckman Coulter). Collected cells were rinsed twice with PBS and stained with Zombie-UV viability dye (BioLegend) ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C for 30 minutes in an OptimaTM MAX Ultracentrifuge (Beckman, Germany). Supernatant was collected and stored at -80°C ...
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bioRxiv - Immunology 2023Quote: ... After centrifugation at 100,000xg for 16 hat 4°C (Beckman Ti50.2 rotor), the density of each fraction was measured ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 hours at 4 °C in an ultracentrifuge (LE-80K, Beckman). The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck) ...
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bioRxiv - Cell Biology 2024Quote: Cell volumes were measured using a Coulter counter (Multisizer 4, Beckman Coulter), which is based on the principle that a cell traversing an orifice filled with electrolyte will produce an impedance change proportional to the volume of the cell due to displacement of electrolyte ...
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 30 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 500 mL hypotonic lysis buffer containing 10 mM Tris-HCl pH 7.8 ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final ultracentrifugation step at 100,000g/1hour/4°C (Beckman AirFuge CLS Ultracentrifuge) resulted in pellets which were resuspended in the same buffer (66) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cell count was acquired using a Multisizer 4 Coulter Counter (Beckman-Coulter) using standard protocols and a threshold of 12 to 30 μm.
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bioRxiv - Cell Biology 2023Quote: ... Gradients were centrifuged at 200,000xg for 16 h at 4°C (Beckman–Coulter Optima™ MAX–E centrifuge ...
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bioRxiv - Cell Biology 2023Quote: ... EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Neuroscience 2024Quote: ... and spun at 4°C in a fixed vertical rotor (VTi50, Beckman) at 242,000 g for 47 min to achieve subcellular fractionation.
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bioRxiv - Cell Biology 2020Quote: ... The resulting supernatant containing AAV particles was added to the top of an iodixanol gradient (15%, 25%, 40%, 60% top to bottom) in an Optiseal tube (Beckman Coulter #361625). The gradient was then centrifuged using a Beckman Ti-70 rotor in a Beckman XL-90 ultracentrifuge at 67,000rpm for 70min ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cancer Biology 2021Quote: ... transferred to 96-well plates and incubated 15 min at room temperature with Agencourt AMPure XP magnetic beads (Beckman Coulter Cat# A63881) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter, Brea, CA, USA). This experiment was repeated 3 times on different days.
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bioRxiv - Neuroscience 2022Quote: ... Cell lysate was then loaded on an iodixanol gradient (60, 40, 25 and 15% iodixanol, ELITechGroup #1114542) in Beckman Quick-Seal Polyallomer tubes (Beckman-Coulter #342414) which were sealed and centrifuged in a Beckman-Coulter Optima XE-90 Ultracentrifuge at 16 °C and 69,000 rpm for 1 hour and 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...
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bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was purified through a 15 to 45% (w/v) sucrose density gradient by ultracentrifugation at 160,000 × g (using a Beckman SW40 Ti rotor) for 2.5 h at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...