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Citations for Beckman :
401 - 446 of 446 citations for H D Glu amc oh since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
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bioRxiv - Cell Biology 2022Quote: ... Cells used for exosome production were incubated in exosome-free medium produced by ultracentrifugation at 40,000rpm for 24 h using a Ti-45 rotor (Beckman Coulter, Brea, CA) in a LE-80 ultracentrifuge (Beckman Coulter) ...
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Isolation and characterization of a novel phage of Vibrio parahaemolyticus with biocontrol potentialbioRxiv - Microbiology 2022Quote: ... for purification and centrifuged at 200,000 × g at 4°C for 24 h using an Optima L-100 XP ultracentrifuge (Beckman Coulter, CA, USA). The purified phage particles were dialyzed through 30-kDa superfilters (Millipore ...
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bioRxiv - Neuroscience 2022Quote: ... The loaded gradient was ultracentrifuged at 69,150 x g for 1 h at 4 °C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. #331362) and the synaptosome fraction was harvested from the interface between the two sucrose layers ...
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bioRxiv - Cell Biology 2022Quote: ... One milliliter of concentrated conditioned medium was pipetted on top of the gradient and samples were centrifuged for 18 h at 100,000 ×g and 4 °C using a SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Fractions of 1 mL were collected from the top and EV-rich fractions 9 and 10 (corresponding to a density of 1.10-1.12 g/mL ...
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bioRxiv - Biophysics 2024Quote: ... The gradients were then centrifuged at 187,000 × g for 4 h at 4°C in a SW 41 Ti rotor (Beckman Coulter, Brea, CA). Bands corresponding to purified virus particles (∼1.36 g/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... ADEV isolation was performed by ultracentrifugation at 100,000 × g for 14 h at 4 °C (swing bucket rotor on minimum acceleration/break setting, SW 32Ti Beckman Coulter, Brea, CA), and ADEVs were resuspended in 200μL PBS and stored at −20°C until used16,41 ...
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bioRxiv - Cell Biology 2023Quote: ... was next subjected to ultracentrifugation at 120,000 × g for 4 h in a SW 28 Ti Swinging-Bucket Rotor (Beckman Coulter, Fullerton, CA) to sediment small EVs ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was segregated subsequent to centrifuging the brain tissue homogenate for 1 h at 100,000g and 4°C utilising an ultra-high-speed centrifuge (Beckman, Optima MAX-XP). The tissue residue was then reprocessed ...
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bioRxiv - Neuroscience 2024Quote: ... The PNS- Nycodenz gradient was centrifuged for 1 h at 141,000 x g at 4°C (46,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Biochemistry 2024Quote: ... The mCherry and GFP double-positive cells were single-cell sorted 24 h post-transfection using an MoFloAstrios EQ cell sorter (Beckman Coulter, US) and grown in separate cultures that were subsequently screened for the presence of frameshift mutations leading to nonsense-mediated decay on both alleles ...
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Physiology 2023Quote: ... and HDL (1.063 <d <1.210 g/mL) using NaBr buffers in the Optima MAX-TL Ultracentrifuge with the TLA-100 rotor (Beckman Coulter). Complete blood count analysis was carried out using Heska Element HT5.
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 150 μl 0.1 M HEPES/NAOH pH 7.0 and purified for 48 h (250,000 × g, 4 °C; Beckman Optima LE-80K, rotor SW60-Ti) using a CsCl gradient (0.45 g/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... previously centrifuged for 16 h at 120,000 g to remove bovine exosomes using 40.1Ti rotor (L-80XP; Beckman Coulter®, Brea, CA, USA).) ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Biophysics 2022Quote: ... EVs were fractionated by OptiPrep density gradient ultracentrifugation (100,000 × g, 18 h, 4 °C) using a SW41 rotor (Beckman Coulter, Fullerton, CA, USA) through a continuous 5–40% OptiPrep (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... the SBV samples were purified via sucrose density gradient centrifugation (10–50% range) at 32,500 rpm for 4 h with an SW 41 Ti Swinging-Bucket Rotor (Beckman Coulter Inc., Brea, CA). The SBV band that corresponded to the 40% sucrose gradient was harvested and then centrifuged at 25,000 rpm for 12 h ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Biophysics 2023Quote: ... Virions were banded by density gradient centrifugation at 152,000 x g for 16 h at 4°C in a SW41 rotor (Beckman Coulter SRL, Milan, Italy). The gradient was then fractionated ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then allowed to grow in flasks for another 48 h before sorting them on a MoFlo Astrios sorter (Beckman Coulter, Pasadena, CA) for the brightest cells ...
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bioRxiv - Biochemistry 2024Quote: ... Lysates were centrifuged at 26,000 xg for 25 min (Sorvall RC 5B Plus) followed by membrane isolation through ultracentrifugation at 55,000 pm for 1 h (Beckman, Type 70 Ti Rotor). Membranes were resuspended in HBS by mortar and pestle at 20 mL/g and snap-frozen in LN2 before storage at -80 °C until further use ...
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bioRxiv - Biochemistry 2024Quote: ... Nucleosome sucrose gradients were then centrifuged at 41,000 rpm and 4°C for 22 h in a SW41 Ti rotor in an Optima L-100 XP ultracentrifuge (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Biophysics 2023Quote: ... Samples were indexed using the IDT for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes) and SPRISelect beads (Beckman Coulter) at a 0.9× ratio were used for cleanup and final size selection ...
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bioRxiv - Immunology 2023Quote: Koff rates were determined as dissociation of reversible pMHC-Streptamers upon addition of D-Biotin in a flow cytometry-based assay (CyAn ADP Lx 9 color flow cytometer, Beckman Coulter) as described previously (44) ...
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bioRxiv - Biophysics 2021Quote: ... Following incubation (37 °C, 20-24 h), the samples were ultracentrifuged (1h, 288’000 x g) (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor) to remove large aggregates ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris was removed by centrifugation at 20,000 rpm at 4°C for 1 h (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 25.50). The supernatant was collected and loaded onto a pre-equilibrated 5 ml His-Trap FF crude column ...
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bioRxiv - Bioengineering 2023Quote: ... and a humidity of 85 % in 48-well Flowerplates containing 800 µL defined CGXII medium with 0.5 % (w/v) glucose inoculated to an OD600 of 1 by using the BioLector microbioreactor for 24 h (Beckman Coulter Life Sciences, Aachen, Germany). Increasing concentrations of ANT (final concentration of 0 ...
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bioRxiv - Biochemistry 2024Quote: ... The samples were then subjected to high-speed ultracentrifugation (100000 g for 1 h) at 20 using an Optima TL-100 benchtop ultracentrifuge (Beckman Coulter, Indianapolis, IN, USA). AgADFs and α-actin alone were processed identically as a controls ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Molecular Biology 2024Quote: ... followed by ultracentrifugation for 15 h at 79 000 x g at 4°C in an Optima L-80 XP Ultracentrifuge (Beckman Coulter SW41-Ti rotor). Starting from the top of the gradient ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Microbiology 2024Quote: ... a single colony was inoculated into MicroScan Neg MIC NF-1J panel using Prompt Inoculation System D (Beckman Coulter Inc., Brea, CA, USA). Inoculated panels were incubated at 35°C for 18 h in MicroScan Walkway40 Plus (Beckman Coulter Inc. ...
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.