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Citations for Beckman :
401 - 450 of 1150 citations for 4 4' Bi 7H benz de anthracene 7 7' dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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Faa1 membrane binding drives positive feedback in autophagosome biogenesis via fatty acid activationbioRxiv - Biochemistry 2023Quote: ... Lysates were cleared by centrifugation (20,000 rpm for 45 min at 4°C in a Beckman SW45Ti rotor). The supernatant was incubated with IgG Sepharose beads (GE Healthcare ...
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bioRxiv - Microbiology 2024Quote: ... The eluent was ultracentrifuged (100,000 ×g, 4°C, 16 h) using Optima L-100 XP Ultracentrifuge (Beckman Coulter) to sediment ribosomes.
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor ...
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bioRxiv - Molecular Biology 2022Quote: ... The elutions were combined and centrifuged at 186,000 x g at 4 °C for 1.5 h in a (TLA-120.1) rotor (Beckman Coulter). Supernatant was discarded and the pellet was resuspended in the RNC buffer at a concentration of ~200 nM ...
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bioRxiv - Molecular Biology 2022Quote: ... Gradients were ultracentrifuged at 28.000g for 3 h at 4°C in a SW 28 Ti rotor (Beckman). 1.13 mL fractions were then collected using the Gradient Station model 153 ...
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bioRxiv - Biochemistry 2022Quote: ... Membrane contamination was removed by centrifugation 100,000 x g 1.25 hours 4°C in an NVT65.2 rotor (Beckman Coulter). The clarified supernatant was concentrated to 50 µl by ultrafiltration (Sartorius Vivaspin Turbo ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 49,100g for 16 to 17 hrs at 4°C in an SW32Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Molecular Biology 2022Quote: ... and spun at 100,000g for 70 minutes at 4°C (Optima XPN-80 Ultracentrifuge, Beckman Coulter, California, USA) in a 45ti fixed angle rotor ...
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bioRxiv - Neuroscience 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% bovine serum albumin (Tocris Bioscience ...
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bioRxiv - Cell Biology 2023Quote: ... 10% and 6% and ultracentrifuged at 187,000xg for 1:30h at 4°C in Sw32.1Ti rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Genomics 2023Quote: ... Extracts were then centrifuged at 148,000 g for 2.5 hours at 4°C using the SW 28 Ti rotor (Beckman). Resulting pellet was resuspended overnight at 4°C in 1.5 ml of HBSS ...
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bioRxiv - Bioengineering 2023Quote: ... at 4°C using an Optima Max Ultracentrifuge and a TLA-55 rotor (Beckman Coulter, Brea, CA, USA). The pellet from this supernatant (i.e. ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondrial membranes were pelleted at 72000 xg for 30 min at 4°C using an optimaTM ultracentrifuge (Beckman) with Beckman rotor (TLA 120) ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were then clarified by centrifugation (100,000 xg, 60 min, 4 °C, JA 30.50 rotor, Avanti JXN-26 centrifuge (Beckman)) before filtering the supernatant through 0.45 μm syringe filters ...
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bioRxiv - Microbiology 2024Quote: ... The cells were collected by centrifugation (8,000 rpm, 10 minutes at 4°C, JLA-10.500 rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2024Quote: ... Following an 18-hr centrifugation at 4°C (44,000 rpm, SW55 rotor, Beckman Optima LE-80 K Ultracentrifuge), 420-μl fractions (1-11 ...
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bioRxiv - Developmental Biology 2024Quote: ... Insoluble material was removed by high speed centrifugation (30,000g, 1 hour, at 4°C, in rotor JA12, Beckman) and by 0.45 µm filtration ...
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bioRxiv - Neuroscience 2024Quote: ... Ultracentrifugation was done at 134,000xg for 4h at 4°C using an SW 41 Ti rotor (Beckman Coulter) in an Optima XE-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... microsomes from the IVT reactions were first recovered by centrifugation at 4°C in the TLA55 rotor (Beckman) for 20 min at 55,000 rpm ...
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bioRxiv - Immunology 2024Quote: ... and 20% OptiPrep density gradient at 100,000 × g overnight and at 4 °C (SW 40 Ti, Beckman Coulter). The following day ...
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bioRxiv - Microbiology 2024Quote: ... cultures were harvested by centrifugation for 30 minutes at 5,000 rpm and 4 °C in an Avanti JXN-26 Floor Centrifuge using the JXN 12.500 rotor (Beckman). The resulting pellets were resuspended in 40 mL Lysis buffer (20 mM HEPES pH 7.5 ...
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bioRxiv - Cell Biology 2024Quote: ... The post-nuclear supernatants were centrifuged at 60,000 rpm for 60 min at 4°C with a TLA 120.2 rotor (Beckman) to collect ER-mitochondria-enriched fractions ...
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bioRxiv - Genomics 2024Quote: ... by centrifuging the chip facing downwards at 3,220 g for 5 min at 4 °C and purified with 1.5 × Ampure XP beads (Beckman), followed by DNA QC and trace checking.
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bioRxiv - Biochemistry 2024Quote: ... and subjected to centrifugation at 35,000 rpm for 2.5 hr at 4 °C using a SW41Ti rotor (Beckman). The polysome profile in sucrose gradients was resolved using a Brandel gradient fractionator ...
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bioRxiv - Cancer Biology 2024Quote: ... followed by ultracentrifugation at 100,000 g for 90 min at 4°C (45Ti or TLA110 rotors, Beckman Coulter). The pellet containing EVs was washed in cold 1x PBS (Gibco ...
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bioRxiv - Biophysics 2024Quote: ... and the lysate was cleared by ultracentrifugation for 30 minutes at 4°C (Ti45 rotor, 37,000rpm, Beckman Coulter). The proteins were purified by affinity chromatography using an ÄKTA Start (GE Healthcare ÄKTA Start) ...
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bioRxiv - Biophysics 2021Quote: ... Cell debris was removed by centrifugation (12,000x□g for 15□min at 4°C) and membranes were then pelleted at 45,000 rpm in a Type 45 Ti rotor (Beckman) for 3□h at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25 000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was incubated with GSH beads (GE Healthcare ...
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bioRxiv - Biochemistry 2021Quote: ... after which the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA 25.50 rotor in an Avanti J-E centrifuge (Beckman). Nucleic acids in the clarified lysate were precipitated by slowly adding polyethyleneimine with constant stirring in an ice bath to a final concentration of 0.4% ...
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bioRxiv - Microbiology 2020Quote: ... of 1.5 mL and then centrifuged 45 min at 100,000g at 4°C in a TLA-100 ultracentrifuge (Beckman Coulter). The supernatant contains the soluble fraction and the pellet corresponds to the total membranes ...
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bioRxiv - Cell Biology 2020Quote: ... 0.5 mM DTT, 100 μg/ml cycloheximide) and centrifuged (160,000 g, 120 min, 4°C) in an SW41Ti rotor (Beckman). After centrifugation the sediment in the tube went through a UV detector at the wavelength of 254nm and simultaneously the required fractions (>=5 ribosomes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The filtered supernatant from one 400 ml batch was then pelleted (1.5 hour at 24 000 rpm at 4 °C with Beckman Coulter L-90K centrifuge and 45 Ti rotor ...
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bioRxiv - Biophysics 2022Quote: ... The sample was loaded on top of the glycerol gradient and centrifuged at 4°C for 18 h at a speed of 35,000 rpm in SW-41Ti rotor (Beckman). Fractions of 500 μL were collected ...
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bioRxiv - Microbiology 2020Quote: ... Phage were pelleted by centrifugation at 82705 xg (25,000 rpm) for 4 hours using an SW28Ti rotor (Beckman Coulter). Phage pellets were gently resuspended overnight in 1 mL of phage buffer at 4°C with shaking ...
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bioRxiv - Genomics 2021Quote: ... we filtered the virus-laden media through a 0.22μm PES filter and then concentrated viral particles by ultracentrifugation at 24,000 rpm for 1.5 hours (4°C) with an SW-32Ti swinging bucket rotor (Beckman Coulter). We resuspended the viral pellet in iPS-Brew at 1/200th the original supernatant volume and then stored 50-100 μl aliquots at −80°C.
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Immunology 2021Quote: Cells were analyzed using 4-to 6-color direct immunofluorescence staining on a Cytoflex LX or S (Beckman Coulter) flow cytometer ...
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bioRxiv - Microbiology 2021Quote: ... 200mM NaCl was used to top up the 13mL polyethylene tube and the solutions were spun at 27,000×g for 18 hours at 4°C using SW45 Ti rotor (Beckman). 1mL of top fraction ...
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bioRxiv - Biochemistry 2020Quote: ... and were centrifuged for 4 min at 46 000 rpm at 4°C in a Optima MAX-XP Ultracentrifuge with a TLA 100.3 rotor (Beckman). The pellet was suspended in (20 µl ...
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bioRxiv - Cancer Biology 2021Quote: ... Libraries were further amplified 4–6 PCR cycles as described [43] and purified using AMPure XP beads (Beckman Coulter). ATAC-seq libraries were subsequently sequenced 2 x 75 bp on an Illumina NextSeq500.
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bioRxiv - Biophysics 2021Quote: ... sucrose density gradients and centrifuged at 24,000 × rpm (102,170 × g) for 18 h at 4°C (Sw40Ti rotor, Beckman Coulter). The fractions of the sucrose gradient were applied to SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... The VP2 capsid protein fractions were pooled and subjected to centrifugation at 28,000 rpm (139,065 × g) for 3 h at 4°C (Sw40Ti rotor, Beckman Coulter). The pellet was resuspended in 50 mM Tris (pH 7.4) ...
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bioRxiv - Biochemistry 2020Quote: ... mixed for 10 minutes at 4°C and centrifuged at 25,000 rpm for 45 minutes (Beckman Ti-45 rotor). The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter, # 331362). The polysome fractions were collected using the Foxy R1 Fraction Collector and immediately snap frozen with liquid nitrogen and stored at −80 °C ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 40,000 rpm (∼128,000 × g) for 3 h at 4°C using an MLS-5 rotor (Beckman Coulter). Aldolase (158 kDa ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Biochemistry 2021Quote: ... 1□mM EDTA) and centrifuged at 25000 rpm for 1 hour at 4°C (Beckman Coulter SW41-Ti rotor). Mitochondria forming a band at the interphase between the 1 M and 1.5 M sucrose were collected and resuspended in 10 mM Tris-HCl pH = 7.5 in 1:1 ratio ...
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bioRxiv - Biochemistry 2020Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 μL of ANTI-FLAG M2 Affinity Gel (Sigma ...