Labshake search
Citations for Beckman :
501 - 550 of 1150 citations for 4 4' Bi 7H benz de anthracene 7 7' dione since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 µL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by extrusion using a Teflon Dounce homogenizer and clarified by centrifugation at 30000g for 40 minutes at 4°C using a Ja 25.50 rotor (Beckman). The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting lysate was cleared by centrifugation at 279,288 × g for 10 minutes at 4°C using a tabletop centrifuge unit from Beckman Coulter ...
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bioRxiv - Genomics 2023Quote: ... followed by centrifugation at 31,900 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). Next ...
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bioRxiv - Neuroscience 2023Quote: ... Samples were then centrifuged at 82,600×g at 4°C for 2 h (Beckman ultracentrifuge optima L-100 XP). After the first ultracentrifugation ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Biochemistry 2023Quote: ... at 40% on a Branson Digital Sonifier and clarified by centrifugation for 30 minutes at 27,216 × g at 4°C in an SS-34 rotor (Avanti J-25 centrifuge, Beckman).
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... placed in an An50Ti rotor and centrifugated 50,000 rpm at 4 °C using an Optima XL-I analytical ultracentrifuge (Beckman). The data were analysed in SEDFIT-16.1c 53 using a c(s ...
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bioRxiv - Cell Biology 2023Quote: ... and 1 mM PMSF) and centrifuged for 15 hours at 4°C with 74,400 xg (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected from the top and subjected to TCA precipitation ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged (33.500 rpm/4°C/3h, brake off) in a Optima XPN-80 ultracentrifuge (Beckman Coulter, Krefeld, Germany). Sucrose gradients were eluted into a BR-188 density gradient fractionation system (Brandel Inc ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Biophysics 2023Quote: ... The supernatant was subjected to a second round of ultracentrifugation at 100,000 x g for 1 hour at 4°C in a Type 45Ti rotor (Beckman) to pellet cell membranes ...
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bioRxiv - Cell Biology 2023Quote: ... 20µM ATP and 1 mM DTT and subsequently sedimented (100,000 x g, 15 min, 4°C, TLA-100 rotor) in an Ultima MAX-XP ultracentrifuge (Beckman). For competition assays ...
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bioRxiv - Microbiology 2023Quote: ... The tube was centrifuged for 3 hours at 150’000 x g (4°C) using a SW 32.1 Ti swinging bucket (369651, Beckman Coulter). Finally ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter ...
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bioRxiv - Systems Biology 2023Quote: ... Two libraries were prepared and sequenced: one with size-based selection for mRNA >4 kb using AMPure beads (Beckman) and a second library with no size selection.
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bioRxiv - Microbiology 2022Quote: ... The gradient was spun at 45,000 rpm (~200,000 x g) for 6 hours at 4 °C in a SW60Ti rotor (Beckman Coulter). Successive 0.4 mL fractions were collected by pipetting and their densities measured ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
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bioRxiv - Cancer Biology 2024Quote: ... 100 μg/mL cycloheximide) for 2 h at 40,000 g at 4 °C using an SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 × g for 1 hour at 4°C (JXN-30 centrifuge, JA-30.50 Ti Rotor, Beckman Coulter). The pellet was washed with PBS and centrifuged again at 100,000 × g for 1 hour at 4°C to remove soluble contaminant ...
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bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2024Quote: ... samples were ultracentrifuged at 160,000 xg for 2 h at 4 °C in ultracentrifuge tubes (Beckman Coulter Cat #328874). The resulting pellet contained the insoluble AMB-1 cell fraction and the supernatant contained the soluble fraction ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 100,000 x g for 2 h at 4 °C to sediment small EVs (Optima L-80K ultracentrifuge, Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1 M sucrose) at 100,000 rpm (543,000 × g) and 4°C for 1 h with a TLA110 rotor (Beckman Coulter) and an Optima MAX-TL Ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...
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bioRxiv - Cell Biology 2023Quote: ... After cell debris and undisrupted cells were removed by centrifugation (45,000 rpm, 30 min, 4°C, 70Ti rotor; Beckman), supernatant was applied to an open column with Ni-IMAC resin (BIO-RAD) ...
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bioRxiv - Neuroscience 2023Quote: ... the liposomes were isolated by centrifugation for 3 h 40 mins at 55,000 rpm at 4°C in a SW 60 rotor (SW 60 Ti, Beckman). 20 µl were harvested from the top of the gradient and mixed with 8 µl of 4x Laemmli buffer (final 62.5 mM Tris-HCl ...
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bioRxiv - Plant Biology 2023Quote: ... The cel lysates were clarified by high-speed centrifugation at 50,000g for 60 min at 4°C (Avanti J-26 XP centrifuge with JA-25.50 rotor; Beckman Coulter), followed by a 0.45-mm filtration (Millex-HP ...
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bioRxiv - Molecular Biology 2024Quote: ... Virus was then purified by centrifugation through a 20% sucrose cushion at 25,000 rpm for 2.5 hours at 4 °C in a Sw32Ti rotor (Beckman Coulter). Virus pellets were resuspended in PBS and virus genomic RNA copy number was quantified by RTqPCR as described above ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and were centrifuged at 39,000 rpm for 2.45 hours at 4°C using Optima XPN-80 Ultracentrifuge (Beckman Coulter). Following centrifugation ...
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Molecular Biology 2023Quote: ... Small extracellular vesicles were pelleted at 100,000 X g for 1,5 h at 4°C by using Ti-45 rotor (Beckman Coulter) and 70 ml polycarbonate bottles (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
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bioRxiv - Cancer Biology 2023Quote: ... the obtained supernatant was filtered through a 0.22 μm filter before ultracentrifugation at 150,000 g for 2 h at 4 °C (Beckman Coulter). The nsEV-containing pellet was resuspended in phosphate buffer saline (PBS).
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bioRxiv - Neuroscience 2023Quote: ... NP-40 prepared in DPBS] and then ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman). The supernatant was removed ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... of proteins were applied on the top of a 16 ml 10-30% continuous sucrose density gradient and centrifuged at 74,400 g for 15 h at 4 °C (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected ...
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bioRxiv - Biochemistry 2023Quote: ... at 18000 PSI and cell debris were separated by centrifugation (4 °C, 30 min, 75,600 ×g, JA25.50 rotor Beckman Coulter). The supernatant was loaded on an affinity chromatography column (GE Healthcare ...
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bioRxiv - Biochemistry 2023Quote: ... The mixture was incubated for 30 min at 4 °C and centrifuged at 100,000 × g at 4 °C for 1 h using a TLA100 fixed angle rotor (Beckman). The supernatant fraction was carefully removed ...
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bioRxiv - Immunology 2022Quote: ... stained bacteria were then sonicated for 4 min (0.5 cycle, 75 A, VialTweeter) followed by determination of concentration using flow cytometry (CytoFlex; Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...