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Citations for Beckman :
351 - 400 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Developmental Biology 2021Quote: 50,000 ES cells and AFK cells at 48h were collected by flow cytometry (MoFlo, Beckman Coulter). Cells washed with ice-cold PBS once then lysed in the buffer (10 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... hPSCs’ viability and counts were determined on Vi-CELL XR Cell Viability Analyzer (Beckman Coulter, 731050). To continue the differentiation protocol viability had to be above 80% ...
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bioRxiv - Genetics 2020Quote: ... Each cell was sorted using a high speed cell sorter (MoFlo XDR; BECKMAN COULTER, Brea, CA) into a 96-well plate with qPCR buffer [SYBR Premis Ex TaqTM (Tli RHaseH Plus)] (RR420A ...
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bioRxiv - Cancer Biology 2020Quote: ... and double positive (cKit+ CD45.2+) cells were sorted using the MoFlo Astrios Cell Sorter (Beckman Coulter). 0.5×106cells were re-suspended in 1ml BMT media ...
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bioRxiv - Cell Biology 2021Quote: ... Individual Venus-positive cells were isolated on a Moflo XDP 100 cell sorter (Beckman Coulter, Inc.) following 5 min of stimulation with 10 ng/mL PDGF-AA or PDGF-BB (R&D Systems ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were lifted by trypsin and counted using the automated cell counter (Z series) by Beckman Coulter ...
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bioRxiv - Cancer Biology 2020Quote: The cell viability after radiotherapy treatment was measured using the Vi-CELL viability analyzer (Beckman Coulter), which performs trypan blue dye exclusion method with an automated system ...
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bioRxiv - Immunology 2020Quote: ... B2M negative cells were selected and bulk-sorted using cell sorter Moflo Astrios EQ (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... GFP-positive cells were sorted into single clones using an Astrios EQ cell sorter (Beckman Coulter). Single clones were cultured in 96-well plates for two weeks ...
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bioRxiv - Evolutionary Biology 2021Quote: ... viability and diameter were performed using a cell counter (Vi-Cell™ XR, Beckman coulter, #731050). MDCK(adh ...
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bioRxiv - Physiology 2021Quote: ... and these cells were pre-sorted with MoFlo Astrios cell sorter (Beckman Coulter, Brea, CA, USA) to rule out the immune- ...
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bioRxiv - Cancer Biology 2021Quote: ... neurospheres were mechanically disaggregated and counted using the Vi-CELL XR cell counter (Beckman Coulter, USA). Glutathione was added at a final concentration of 0.5 mM ...
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bioRxiv - Cancer Biology 2022Quote: ... Viable cell counts were measured in duplicate with a ViCell XR cell viability analyzer (Beckman Coulter).
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bioRxiv - Developmental Biology 2023Quote: ... Cell sorting was performed in a high speed cell sorter (MoFlo Astrios EQs, Beckman Coulter, USA) and analyzed with FlowJo software (Flow Jo).
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bioRxiv - Immunology 2022Quote: 40’000 CD45+ cells and 40’000 total live cells were sorted on a MoFlo Astrios (Beckman Coulter) and collected in separated 0.2mL PCR tubes containing 10μl in PBS + 0.04% BSA + 0.1% RNasin ...
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bioRxiv - Immunology 2023Quote: Total cell counts per spleen were determined via the Vi-Cell counter (Beckman Coulter, Fullerton, CA) (16) ...
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bioRxiv - Biochemistry 2023Quote: ... single-cell sorting was performed on a MoFlo Astrios cell sorter (Beckman Coulter, Brea, CA, USA) equipped with 355- and 488-nm lasers ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were then trypsinized and single cells were sorted using the MoFlo Astrios EQ (Beckman Coulter) cell sorter at the Cytometry Shared Resource Laboratory (CTEGD ...
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bioRxiv - Cell Biology 2023Quote: ... The cell cycle distribution of cell populations was analyzed with a flow cytometer (Cytoflex, Beckman Coulter). Generated .fcs files were analyzed and visualized by FlowJo (v10.8.1).
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bioRxiv - Biophysics 2024Quote: NK-92 cell viability was determined using trypan blue exclusion assay and Vi-cell counter (Beckman). For NK-92 cytotoxicity assay with DAOY medulloblastoma cells ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were sorted for CD184+/CD44-/CD271-/CD24+37 in MoFlo XDP Cell Sorter (Beckman-Coulter) at the SickKids Flow Cytometry facility into Matrigel (Corning ...
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bioRxiv - Immunology 2024Quote: ... Cell suspensions were prepared for subsequent cell sorting in a MoFlo Astrios EQ FACS-sorter (Beckman) to isolate nN1 ...
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bioRxiv - Cell Biology 2020Quote: ... Cell apoptosis were analyzed by Beckman Cytoflex (Beckman Coutler Co.Ltd &Cytoflex).
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bioRxiv - Bioengineering 2019Quote: ... The Vi-Cell XR (Beckman Coulter) was used to quantify viable cell counts and 6 × 105 cells/mL in mTeSR1+ 10 μM Y27632 (Abcam ...
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bioRxiv - Microbiology 2023Quote: ... MoFlo Astrios cell sorter (Beckman Coulter) was used to enrich GFP positive parasites with the top 5% tdTomato-ATG8 signal ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation (Beckman Coulter ...
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bioRxiv - Microbiology 2024Quote: ... Cell fluorescence was detected by Beckman Coulter Cytoflex S and analyzed using FlowJo software.
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bioRxiv - Biochemistry 2022Quote: ... 750 µL of the 80% Histodenz solution were added and thoroughly mixed with 750µL of the protein-LUV sample in Thinwall centrifuge tubes (Beckman 50.2ti rotor). 1.5 mL of the 30% Histodenz solution was added as the intermediate layer ...
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bioRxiv - Biochemistry 2020Quote: ... Protein samples were clarified by centrifugation using a TLA-55 rotor in an Optima MAX-XP table top ultracentrifuge (Beckman Coulter) (90,720 x g ...
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bioRxiv - Immunology 2022Quote: ... and the expression of the Gag- p24 viral protein was detected with a PE-anti-p24 antibody (clone KC57 RD1; Beckman Coulter). Cell viability was determined using an aqua viability dye for flow cytometry (LIVE/DEAD® Fixable Aqua Dead Cell Stain kit ...
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bioRxiv - Biochemistry 2023Quote: The tendency of the protein to form aggregates was determined by dynamic light scattering (DLS) in the Delsa Max Core (Beckman Coulter). Prior to measurement ...
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bioRxiv - Pathology 2023Quote: ... the proportion of HIV-1-infected cells was determined by intracellular staining of the viral protein Gag-p24 with a Mouse anti-p24-FITC antibody (clone KC57, Beckman Coulter).
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Cell Biology 2019Quote: ... Sucrose gradients were subjected to ultracentrifugation (SW41 Ti 11E1698 rotor; Beckman at 260,000xg for 2 h at 4□°C) and fractionated by displacement using 60% sucrose/0.01% bromophenol blue on an ISCO Foxy fraction collector (35 s for each fraction ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The functional complex was recovered as a jelly-like pellet by centrifugation at 60,000 rpm at 25°C for 2 h in an ultracentrifuge (128,000 g in a TLA 120.2 Beckman rotor) and packed into a 1.9 mm rotor ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
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bioRxiv - Cell Biology 2019Quote: ... MT seeds were diluted in BRB80 containing 2 μM taxol and centrifuged at 100,000 g (Optima TL100; Beckman Coulter) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was cleared again by 2 rounds of ultracentrifugation at 100,000 rpm (358,400 x g; Beckman TLA-120.2) for 10 minutes at 0°C ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Molecular Biology 2021Quote: ... and the tube was centrifuged for 2 hours at 190,000 x g in a swing-out rotor (SW40 Ti; Beckman Coulter ...