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Citations for Beckman :
501 - 550 of 1842 citations for Dengue Virus Serotype 2 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µl 2% PBS-BSA and analyzed for mean fluorescence intensity (MFI) of ACE2 expression with CytoFLEX flow cytometer (Beckman). To determine the biological effects of the purinergic receptor P2X7 and NLRP3 inflammasome on viral entry ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were ultracentrifuged at 35,000 rpm for 2 hrs at 4°C using an SW41 Ti rotor (Beckman Coulter). The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO ...
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bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Genomics 2020Quote: ... cells were harvested and analyzed by Beckman Coulter CytFLEX (Beckman Coulter ...
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bioRxiv - Immunology 2020Quote: ... the cells were permeabilized (Beckman Coulter kit) and stained with p-(S465/S467)-Smad2/p- (S423/S425)/Smad3-Alexa 647 mAb Phosflow antibody (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... A MoFlo Astrios cell sorter (Beckman Coulter), with gating for live IgM+/CD20+/CD3-/CD4-/CD8-/CD14-/CD16- cells ...
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bioRxiv - Cancer Biology 2020Quote: ... using the MoFlo cell sorter (Beckman Coulter). Single cells were cultured in the presence of CTC media containing either 1μM of P1C2 ...
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bioRxiv - Microbiology 2021Quote: ... Cells were centrifuged (Avanti JXN-26 Beckman Coulter Centrifuge ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were analyzed by Cytoflex (Beckman) flow cytometer.
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bioRxiv - Biochemistry 2021Quote: ... Stained cells were analyzed using CytoFLEX (Beckman) instrument.
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bioRxiv - Cancer Biology 2023Quote: ... or MoFlo XDP Cell Sorter (Beckman Coulter).
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bioRxiv - Bioengineering 2023Quote: ... before cell pelleting by centrifugation (Beckman Coulter) at 4,000 x g for 10 minutes at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Cells were centrifuged at 4,000 rpm (Beckman Coulter ...
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bioRxiv - Cancer Biology 2024Quote: The Vi-cell reagent Kit (Beckman Coulter) was used according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2019Quote: ... Membranes and other cellular debris were sedimented away from soluble protein on a Beckman J2-21 large floor centrifuge (Beckman Coulter, Brea, CA). Supernatant containing the recombinant protein was incubated with 10 mL of precleared Ni-NTA beads for 10min at 4°C ...
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bioRxiv - Immunology 2020Quote: ... CD103+ CD11b+ cDCs were Fluorescence-activated cell sorting (FACS)-sorted with the MoFlo Astrios EQ cell analyzer (Beckman Coulter).
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bioRxiv - Developmental Biology 2020Quote: ... filtered through a 70 μm cell strainer and stained with the dead cell marker Draq7™ (Beckman Coulter, #B25595). Viable single cells were collected on a BD FACS Aria II by excluding debris (side scatter vs ...
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bioRxiv - Immunology 2021Quote: ... NK cells were isolated as above and then sorted on MoFlo XDP cell sorter (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Immunology 2020Quote: ... cell concentrations were adjusted to 107cells/mL and sorted with a MoFlo AstriosEQ high speed cell sorter (Beckman Coulter) according to our previously published protocol [18] ...
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bioRxiv - Cell Biology 2023Quote: ... cell volume distributions and cell numbers per mL were determined using a Coulter Counter (Beckman Coulter, Z2 Particle Counter). From each culture ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 20nM of gemcitabine and cells were counted 3 days later using the Z1 Coulter (Beckman).
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bioRxiv - Cell Biology 2023Quote: ... After cell debris and undisrupted cells were removed by centrifugation (45,000 rpm, 30 min, 4°C, 70Ti rotor; Beckman), supernatant was applied to an open column with Ni-IMAC resin (BIO-RAD) ...
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bioRxiv - Immunology 2023Quote: ... White blood cell (WBC) counts were measured from blood with an automatic cell counting machine (DxH 500, Beckman Coulter). Animals were euthanized when they met pre-defined termination criteria defined in the animal license.
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bioRxiv - Synthetic Biology 2024Quote: ... Cells were then filtered through a 40 μm cell strainer and analyzed using a CytoFLEX S instrument (Beckman Coulter). We used the self-build python code to analyze the flow data.
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bioRxiv - Cell Biology 2023Quote: ... Individual Venus-positive cells were isolated on a Moflo XDP 100 cell sorter (Beckman Coulter Inc., Brea, CA, USA), following 5 min of stimulation with 10 ng/ml PDGF-BB (R&D Systems ...
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bioRxiv - Molecular Biology 2024Quote: Cells were sorted at the NINDS Flow and Imaging Cytometry Core Facility on the MoFlo Astrios Cell Sorter (Beckman Coulter Life Sciences ...
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bioRxiv - Microbiology 2024Quote: ... The percentage of GFP-positive cells was determined by analyzing 20,000 cells on a FC500 MPL cytometer (Beckman Coulter) using the CXP Software (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Neuroscience 2019Quote: ... with 2 ml of a 20% sucrose in dPBS cushion and ultracentrifugated at 20,000 RPM (Beckman Coulter SW 32 Ti rotor) at 4°C for 2 hours ...
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bioRxiv - Cell Biology 2019Quote: ... The culture was harvested in to 2 × 250 ml centrifuge bottles (Nalgene, 3120-0250) and centrifuged in an Optimia XPN-100 ultra centrifuge (Beckman Coulter) with a SW 32 Ti rotor at 5,000 × g for 20 min at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cancer Biology 2019Quote: ... Gradients were ultra-centrifuged at 221,600 × g for 2 hours at 4°C (SW-41Ti rotor, 331362, Beckman Coulter, Brea, CA). Gradients were fractionated (Piston Fractionator ...
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bioRxiv - Genomics 2019Quote: ... Cleared cell extracts were ultracentifuged at 34400 rpm for 2 hours 40 minutes at 4C using a C-1000 XP centrifuge with SW40 rotor (Beckman Coulter). Gradient UV absorption at 254 nm was measured and selected fractions were selected for 5’cap library preparation (5µg purigied RNA per sample) ...