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Citations for Beckman :
301 - 350 of 547 citations for 3 7 Diethyl 2 hydrazinoquinoline hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... in 2%BSA and 2mM EDTA and sorted using FACS sorter (Beckman Coulter, MoFlo Astrios Zellsortierer). For gating ...
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bioRxiv - Immunology 2023Quote: Cells were washed with 2% FACs and data was acquired using CytoFLEX S (Beckman Coulter, USA) and analyzed via FlowJo v10 software (Oregon ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 2% BSB-PFA and analysed by Flow Cytometry on BD Celesta instrument (Beckman Coulter) using FlowJoTM software ...
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bioRxiv - Developmental Biology 2024Quote: ... EVs were pelleted by ultracentrifugation at 55,000 × rpm for 2 hours using the TLA120.4 rotor (Beckman). The EV pellet was resuspended in 1XPBS and frozen at −80 °C until further analysis.
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bioRxiv - Genetics 2024Quote: ... The filtered medium was centrifuged at 19400 rpm and 17 °C for 2 h (Beckman Coulter). The pellet was suspended in 250 µL HBSS (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... then trypsinized and resuspended in PBS with 2% FBS for quantification using a flow cytometer (Beckman). JC-1 monomers and aggregates were detected at PE and FITC channels ...
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bioRxiv - Biophysics 2022Quote: ... The fractions containing monomeric peptide were pooled and centrifuged at 386000 g for 3 h in an Optima TLX ultracentrifuge equipped with a TLA 120.1 rotor (Beckman Coulter, Atlanta, GA, USA). Orthophosphoric acid or sodium phosphate were added to a final concentration of 20 mM to adjust the pH to 2.8 or 7.4 ...
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bioRxiv - Biochemistry 2021Quote: The absorbance spectra of recombinant DosS and DosT (3 µM) were determined at room temperature using quartz cuvettes with rubber stoppers in a DU800 spectrophotometer (Beckman Coulter, Fullerton, CA). The reduction of recombinant DosS was achieved via addition of sodium dithionite (DTH ...
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bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
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bioRxiv - Bioengineering 2023Quote: ... the supernatant was then taken and loaded into a 10% to 45% sucrose gradient solution and centrifuged at 360000 rpm for 3 h (Beckman Optima XE-100). 18 fractions from each sample were collected using a piston gradient fractionator (Biocomp ...
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bioRxiv - Microbiology 2024Quote: The virus solutions were centrifuged at 106800 × g for 3 hours at 4 °C using a SW32Ti rotor (Beckman Coulter, Fullerton, CA, USA). The virus pellets were suspended in phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2024Quote: ... aliquots of the cultures were fixed in 70% ethanol to analyze cell mass using the Multisizer 3 Coulter counter (Beckman Coulter, Brea, CA). The remaining cultures were further incubated for 4 h with 0.3 mg/mL rifampicin and 0.01 mg/mL cephalexin to allow run-out replication of chromosomal DNA ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Bioengineering 2024Quote: ... using C4F10-saturated PBS until the subnatant was clear after which MBs were counted using a Coulter Counter Multisizer 3 (50 µm aperture tube, Beckman Coulter, Mijdrecht, the Netherlands). Next ...
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bioRxiv - Neuroscience 2020Quote: ... and were centrifuged at 82,500 x g for 2 h at 4 °C (Beckman SW28 swing rotor). The band between 1.0 and 1.2 M sucrose was collected as the synaptosome fraction and diluted with 80 mM Tris-HCl (pH 8.0) ...
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bioRxiv - Pathology 2021Quote: ... BALF (10 μl) was applied to glass microscopy slides using a cytocentrifuge (StatSpin CytoFuge 2, Beckman Coulter). Slides were fixed with 10% neutral buffered formalin ...
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bioRxiv - Evolutionary Biology 2020Quote: ... in 67 mM HEPES-KOH pH 7.2) after 2 h of ultracentrifugation (4 °C, 5000 RPM, Beckman SW41 ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Microbiology 2022Quote: ... 2 ng of each product were pooled for subsequent cleanup using the AmpureXP PCR cleanup protocol (Beckman). The pooled library was quantified by a picogreen assay and loaded onto an Illumina MiSeq Reagent Kit v3 (600-cycle ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was ultracentrifuged for 2 h at 150,000 x g in an Optima XP centrifuge (Beckman) with a MLA-50 rotor ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in 2% FBS-PBS and analyzed by a CytoFLEX flow cytometer (Beckman Coulter, USA). The final results from three independent experiments were averaged and shown in the histogram.
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bioRxiv - Cancer Biology 2024Quote: ... centrifuged twice at 100,000 × g for 2 h at 4°C (Beckman Coulter Optima XE-90 Ultracentrifuge). The supernatant was replaced with DPBS between ultracentrifugation spins ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then resuspended in PBS + 2% FBS and acquired on a Gallios flow cytometer (Beckman Coulter).
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bioRxiv - Molecular Biology 2023Quote: ... followed by ultracentrifugation at 70k RPM at 4⁰C for 2 h (Optima TLX ultracentrifuge, Beckman Coulter). Ribosome pellets were then resuspended in Trizol and extracted for RNA according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Biochemistry 2023Quote: ... The cell culture microplate was centrifuged at 300g for 2 min (Beckman Coulter Allegra X-22R Centrifuge) to bring the cells to the well bottom ...
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bioRxiv - Biophysics 2024Quote: ... Cells were pelleted from 2 L cultures by centrifugation at 4,785g (5,000 rpm in Beckman JLA-8.100) for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... and gradients were centrifuged at 35,000 RPM for 2 hours 45 min using a SW41Ti rotor (Beckman). Absorbance was followed at 254 nm ...
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bioRxiv - Microbiology 2024Quote: ... at 28 K rpm for 2 h at 4°C in an SW32 swing-bucket rotor (Beckman). SG3ΔEnv virions were thoroughly resuspended using 500 μl Hepes/Salt buffer per tube ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was ultracentrifuged at 200,000 xg for 2 hr (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge ...
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bioRxiv - Genomics 2024Quote: ... the virus was ultra-centrifuged at 24,000 rpm at 4 C° for 2 h using SW32Ti (Beckman) and the virus pellets were resuspended in RPMI media with 10% FBS to result in a 20-fold concentrated virus and stored at −80 C°.
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2023Quote: ... CD27 and BA.1 or BA.2 were sorted using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were collected by Summit 6.0 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Digested extracts were then loaded on 1 M sucrose cushions 116 and centrifuged at 70,000 rpm for 2 hours at 4°C in a TLA100.4 rotor (Beckman). Ribosome pellets were resuspended in 300 µl of TRIzol reagent (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Physiology 2024Quote: ... was analysed via a sequential 2-step immune-enzymatic assay (inter-assay CV 5.2-7.8%) according to manufacturer’s instructions (Beckman Coulter) and follicle stimulating hormone (FSH ...
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bioRxiv - Immunology 2024Quote: ... The whole cell lysate was sonicated for 2 min and the cell lysate was centrifuged for 60 min at 20,000 rpm (JA 25.50 rotor, Beckman). The recombinant P72 protein in the supernatant were collected and applied to the anti-HA Magnetic Beads (MCE ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl of the sample pre- and post-bead clean-up (SPRIselect beads, Beckman Coulter, cat. no. B23317) were run in the TapeStation D1000 as a quality control to check peak size ...
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bioRxiv - Cell Biology 2024Quote: ... MKs were subsequently fixed with 2% paraformaldehyde and activation measured using the CytoFLEX flow cytometer apparatus (Beckman Coulter). Normalized mean florescence intensity (MFI ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...