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Citations for Beckman :
451 - 500 of 547 citations for 3 7 Diethyl 2 hydrazinoquinoline hydrochloride since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2024Quote: ... The standard GE-caveospheres and the disrupted GE-caveospheres were centrifuged at 120,000g for 2 hours in an TLA-55 Fixed-Angle Rotor of Optima MAX-XP Ultracentrifuge (Beckman coulter) followed by imaging of fluorescence using a ChemiDoc MP Imaging System as above ...
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bioRxiv - Cancer Biology 2024Quote: ... The gradients were centrifuged at 40,000 rpm for 2 h at 4°C using a SW40 Ti rotor (Beckman Coulter). Eleven fractions of 500 µl each were collected and 200 µl and 50 µl were used for RNA and protein analysis ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant from the 10,000xg spin was layered on top of a 2 mL 60% iodixanol cushion and spun for 4 hours at 100,000xg (SW32 Ti rotor, Beckman Coulter). The bottom 3 mL of the cushion was obtained and layered at the bottom of a discontinuous gradient ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We combined the 8 wells from column 2 of each plate and used the pooled culture to measure cell density on a Coulter Counter Z2 (Beckman Coulter). We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The donor-derived LT-HSC cell populations were sorted according to the gating strategy in Extended data Figure 2 using MoFlo Astrios Cell Sorter (Beckman Coulter). LT-HSC cells were directly sorted into 50μl RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... Samples were incubated for 2 h at 16°C and double stranded cDNA was purified using AMPure XP beads (Beckman Coulter). For testing transcription activity of predicted genes ...
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bioRxiv - Cell Biology 2021Quote: ... to remove cellular debris and purified by ultracentrifugation over a 25% sucrose cushion (28.000 rpm for 2 hours; Beckman Coulter ultracentrifuge).
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bioRxiv - Cancer Biology 2021Quote: ... Cells were treated with 3 different doses of the conventional chemotherapy for 2 days and cell viability was assessed using a Vi-cell XR Viability Analyzer (Beckman Coulter); 6 replicates were performed for each cell line and each dose.
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bioRxiv - Genetics 2020Quote: ... Reverse crosslinking reactions were incubated 2 h at 65°C and cleaned using Agencourt Ampure XP SPRI beads (Cat #A63880, Beckman Coulter) with a 2x bead:sample ratio ...
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bioRxiv - Immunology 2021Quote: ... Beckman Coulter Access SARS-CoV-2 IgG assays (Brea, CA, USA) targeting the RBD were performed on UniCel DxI 800 Access Immunoassay System (Beckman Coulter). Index value threshold for positivity was 1 as recommended ...
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bioRxiv - Immunology 2020Quote: ... erythrocytes in 2 μl blood (collected from the tail tip) were counted using a Z2 Coulter Particle count and size analyser (Beckman Coulter). We determined the healthy range in uninfected C57Bl/6J mice housed in our facility to be 8.8 - 10.5*109 erythrocytes*ml−1 ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 or SW28 rotor (Beckman, Indianapolis, IN). Alternatively ...
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bioRxiv - Immunology 2020Quote: ... 2) first-round PCRs were set up using a Biomek FX liquid handling system with a 96- well head (Beckman Coulter); 3 ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2023Quote: ... viruses were collected and concentrated by ultracentrifugation (37,000 rpm, 2 h 30, 15 °C; Beckman Type 45 Ti fixed-angle rotor). The virus titer was determined using a plaque assay.
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bioRxiv - Microbiology 2023Quote: ... the DNA monitored on 2% agarose gel was purified by means of AMPure XT beads (Beckman Coulter Genomics, Danvers, MA, USA) and quantified by Qubit (Invitrogen ...
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A laboratory framework for ongoing optimisation of amplification based genomic surveillance programsbioRxiv - Genomics 2023Quote: ... PCR amplicons for pools 1 and 2 were combined for each sample and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30μL of sterile water ...
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bioRxiv - Genomics 2024Quote: ... The crude nuclei were stained with 4,6-diamidino-2-phenylindole (DAPI) and loaded into a flow cytometer (Beckman Coulter, MoFlo XDP). Nuclei were purified by flow sorting and washed as described previously [61] ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Neuroscience 2023Quote: ... After PCR the samples were combined into small pool of 2-6 samples and size selection was carried out by adding 0.55x volume of AMPure XP beads (Beckman Coulter, #A68831). The concentrations of purified library pools were quantified with Collibri™ Library Quantification Kit (ThermoFisher ...
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bioRxiv - Biochemistry 2024Quote: ... 50 mM Tris-HCl pH 7.4) and ultracentrifuged at 250 000 x g for 2 h (Beckman Optima TL 100 Ultracentrifuge). After ultracentrifugation ...
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bioRxiv - Cell Biology 2022Quote: ... A 12 ml 2 % step Optiprep gradient in LB ranging from 32 % to 10 % was prepared beforehand in Ultra-Clear tubes (Beckman Coulter). Samples were spun for 16 hours at 100000 g at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
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bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
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bioRxiv - Biophysics 2023Quote: ... and 12-fold diluted in ice-cold SCL and subjected to ultracentrifugation at 100 000 x gav for 2 h in a Ti 70 fixed-angle rotor (Beckman Coulter). The resulting pellets were resuspended in SCL and freshly used for downstream analysis and never subjected to freezing and thawing.
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bioRxiv - Cell Biology 2024Quote: ... to a volume of ∼12 mL with PBS and separated by ultracentrifugation at 160.000 × g for 2 h (4 °C) in a SW Ti41 rotor (Beckman Coulter #331362). Top-layer LDs were collected by aspiration using a pre-wetted 200 µL pipette tip so that maximal LDs were collected in minimal buffer volume ...
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bioRxiv - Molecular Biology 2024Quote: ... lysed by sonication and ultracentrifuged at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). The clarified lysate was loaded onto pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 26K RPM at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell-free extract so prepared was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was subjected to ultracentrifugation at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell lysate was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Microbiology 2024Quote: ... Four working concentrations were achieved through 1:2 serial dilutions using a Biomek i7 liquid handler (Beckman Coulter, ED. Fig. 1a). Precultures were grown overnight and diluted to an OD600 nm of 1 (WT ...
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bioRxiv - Microbiology 2024Quote: ... Particles from the filtrate were concentrated by ultracentrifugation at 100,000 x g for 2 h at 4°C using an SW32Ti rotor in an Optima LE-80K centrifuge (Beckman Coulter). The pellets from different centrifuge tubes were pooled and resuspended in 1 mL of 1X PBS and pelleted again by ultracentrifugation at 165,700 x g for 2 h at 4°C in a TLA-55 rotor (Beckman Coulter) ...
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bioRxiv - Biochemistry 2024Quote: ... 1 mL reaction buffer containing substrates (25 μM-0.16 mM NADH and 2 mM pyruvate) was added to the cuvette of a spectrophotometer (DU® 700, Beckman Coulter). The reaction was started by adding cell lysate ...
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bioRxiv - Genomics 2024Quote: ... We then pelleted this concentrate by centrifugation in a swinging bucket centrifuge (25,000g, 2 hours, 4°, Beckman Coulter SW 32 Ti) and resuspended it in cold PBS + 4% glucose ...
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bioRxiv - Cancer Biology 2024Quote: ... The exosome pellet was resuspended in 35 ml of 0.2 μm-filtered 1x PBS and collected by ultracentrifugation at 100,000 × g for 2 h (45ti rotor, Optima L-100 XP Centrifuge, Beckman Coulter, USA). Exosome pellets were resuspended in 200-300 µL of 0.2 μm-filtered 1x PBS and stored at -80°C ...
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bioRxiv - Biophysics 2024Quote: ... SV experiments were performed at 20°C by UV intensity detection using a 4-hole An-60 Ti rotor and standard 2-channel 12-mm Epon charcoal-filled centerpieces (Beckman Coulter). Samples were run at 26,000 rpm ...