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Citations for Beckman :
301 - 335 of 335 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... was subjected to centrifugation at 100,000g for 2 h in an Optima MAX-XP benchtop ultracentrifuge with a TLA-100.3 rotor (Beckman Coulter, Inc., Brea, CA). Pellets were resuspended in 100 µl PBS and stored at −80 °C.
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bioRxiv - Pathology 2020Quote: ... Hemocytes stained with 4’-6-diamidino-2-phenylindole (DAPI) were analyzed on a CytoFLEX flow cytometer with CytExpert software (Beckman-Coulter, USA). Plots of side scatter SSC-A versus forward scatter FSC-A were used for visualization of cell groups ...
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bioRxiv - Neuroscience 2020Quote: ... Homogenates were layered on top of 1 ml of 0.85 M sucrose in 2 mM EGTA and centrifuged at 75,000 g for 30 min in a Beckman FW-27 rotor (Beckman, Fullerton, CA, USA). This step was repeat twice ...
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bioRxiv - Microbiology 2021Quote: ... soil was thoroughly mixed with 20 ml of 0.01 M CaCl2 for 30 min on a Elmi Intelli-Mixer RM-2 (Elmi Tech Ltd, Latvia) and subjected to centrifugation in an Allegra X-22R centrifuge (Beckman Coulter, USA) at 3900 rcf for 20 min and to remove inorganic S (Dedourge et al. ...
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bioRxiv - Microbiology 2023Quote: ... the prepared DNA library was size-selected on a 2% agarose gel (410–470 bp) using the Agencourt SPRI system to reduce mitochondrial contamination (Beckman Coulter, Inc.). After library prep ...
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bioRxiv - Cell Biology 2023Quote: ... cells were blocked in 2% human serum and PBS for 30 min at 4°C and stained with DC-SIGN (Beckman Coulter #A07407), CD80 (BioLegend #305214) ...
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bioRxiv - Genetics 2023Quote: ... in-house VLPs were produced using the same protocol followed by ultracentrifugation at 24,000 RPM for 2 hours (SW 28 Beckman swinging bucket rotor) to concentrate the VLPs and avoid diluting the HSPCs culture media ...
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bioRxiv - Immunology 2023Quote: ... Bacteria cultures were collected and discarded by centrifugation for 60 min at 4,000-8,000 x g and the supernatants were subjected to high-speed centrifugation at 11,9000 x g for 2-3 h at 4°C (Beckman Coulter Optima Ultracentrifuge). The pellets containing the OMVs were washed with PBS ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Plant Biology 2023Quote: ... 700 μl of obtained extract was applied to a chilled sucrose density gradient and centrifuged at 4 °C for 2 hours 45 minutes at 151,000 ×g using the SW 41 Ti rotor (Beckman Culture Life Sciences). After centrifugation ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were loaded onto the sucrose gradients and centrifuged at 40’000 rpm for 2 h at 4°C using the SW 41 Ti rotor (Beckman Coulter, Cat# 331362). Subsequently ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were centrifuged at 100,000 × g for 2 hr at 4 °C (Type 50.2 Ti Fixed angle ultracentrifuge rotor, Beckman Coulter, Brea, CA, USA). The supernatants were aspirated ...
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bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... and extracellular vesicles including exosomes and virions were pelleted by ultracentrifugation at 141,000 g at 4 °C for 2 h in a swinging-bucket rotor (SW28; Beckman Coulter, Brea, CA, USA). The pellet was resuspended in filtered PBS and centrifuged at 18,300 g and 4°C for 10 min to remove insoluble aggregates ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...
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bioRxiv - Genomics 2020Quote: ... were layered onto a 1.8M sucrose cushion and ultra-centrifuged at 24,400 rpm at 4°C for 2 hours using a SW28 swinging bucket rotor (Beckman Coulter Optimal centrifuge #L90K). The nuclear pellet was re-suspended in 2% BSA in 1X PBS ...
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bioRxiv - Microbiology 2020Quote: ... EMVs were obtained by ultracentrifugation from 4 mL of filtrate at 100,000 × g (average centrifugal force) and 4°C for 2 h with an OptimaX centrifuge (Beckman Coulter, Brea, CA, USA). The pellets were resuspended in 400 μL of Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2022Quote: ... The tubes were sealed and centrifuged at 50,000 rpm at 4°C for 2 h in an OptimaTM L-90K ultracentrifuge using the 70.1Ti rotor (Beckman Coulter, Brea, CA, USA). After the centrifugation ...
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bioRxiv - Cell Biology 2020Quote: ... 3 μM or 10 μM from a 2 mM or 10 mM stock solution using a Biomek FX with 384-well head (Beckman Coulter, Indianapolis, IN). The DMSO concentration was kept below or at 0.5% v/v ...
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bioRxiv - Physiology 2022Quote: ... the library preparation step followed the CEL-Seq 2 protocol85: the RT product was digested by ExoI and HinFI and purified using AMpure XP beads (Beckman Coulter™, USA). Then ...
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bioRxiv - Microbiology 2024Quote: ... then the colonies were diluted and inoculated into the Neg MIC EN-2J panel containing β-lactams listed in Table 2 and incubated in MicroScan Walkway40 Plus (Beckman Coulter Inc.). Breakpoint criteria for susceptible (S) ...
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bioRxiv - Molecular Biology 2024Quote: ... Each compound was dissolved in DMSO at a concentration of 2 mM and distributed into an Echo Qualified 384-Well Polypropylene Microplates (Beckman Coulter, 001-14615). Using an Echo 655 Liquid Handler ...
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bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
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bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
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bioRxiv - Microbiology 2022Quote: ... The success of the amplifications was checked in a 2% agarose gel and PCR products were subsequently purified using Agencourt AMPure XP magnetic beads kit (Beckman Coulter, Brea, CA, USA), quantified with the QuantiFluor™ dsDNA System (Promega ...
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bioRxiv - Microbiology 2020Quote: ... and layered on top of a two-step sucrose gradient (20%/50% w/v in HNE) and centrifuged for 2 h at 154 000 x g at 4 °C in a SW41 rotor (Beckman Coulter, Woerden, the Netherlands) to clear from cell debris ...
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bioRxiv - Microbiology 2023Quote: The amplicons obtained with each of the strategies (using pools 1 and 2) were pooled in each group and purified with one volume of AMPure XP beads (Beckman Coulter, Brea, CA, USA). The beads were washed with 80% ethanol ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Microbiology 2024Quote: ... EMVs were obtained by ultracentrifugation from 3 mL of filtrate at 100,000 × g (average centrifugal force) and 4 °C for 2 h with 50Ti rotor and Optimax centrifuge (Beckman Coulter, Brea, CA, United States). The supernatant after removing EMVs was collected as PVF ...
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bioRxiv - Cancer Biology 2020Quote: ... Filtered media containing lentiviral particles were centrifuged at 24000rpm at 20°C for 2 hrs in 90Ti rotor in ultra-centrifuge (Beckman Coulter Optima TM L-100K). Viral particle pellet was obtained after discarding supernatant post centrifugation and suspended in 100 μl OptiMEM media for overnight at 4°C.The collected lentivirus suspension was used for transfection or stored for later use at −80°C ...
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bioRxiv - Physiology 2020Quote: ... one bird per pen in Experiment 2) and centrifuged for 5 min at 17013 × g using a Beckman microfuge (Model E 348720, Beckman Instruments, INC, Palo Alto, CA). Then the viscosity of ileal supernatant was measured using a Brookfield cone-plate digital viscometer (Model LVDV-Ⅲ ...
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bioRxiv - Microbiology 2024Quote: ... Virus was collected 48 hours post-transfection and pelleted over a 10% sucrose cushion at 25,000 RPM for 2 hours at 4 °C using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Pellets were resuspended in DMEM (Gibco ThermoFisher Scientific ...
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bioRxiv - Microbiology 2024Quote: ... Viruses were collected 48 hours post-transfection and pelleted in DPBS over a 10% sucrose cushion at 25,000 RPM for 2 hours using a SW32Ti rotor (Beckman Coulter Life Sciences, Brea, CA, USA). Virus pellet was then resuspended in labeling buffer (50 mM HEPES pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).