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Citations for Beckman :
201 - 250 of 335 citations for 2 Pyridinesulfonic acid methyl ester since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Cell Biology 2024Quote: ... Gradients were centrifuged at 36,000 rpm for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Following centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The same amounts of proteins were loaded on a double sucrose cushion (20% and 30% sucrose) and centrifuged at 190,000 g for 2 h at 4 °C in an Optima L-100XP ultracentrifuge (Beckman–Coulter). Extracts were incubated with pre-washed anti-Flag beads (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The samples were sedimented by velocity centrifugation at 36,000 x rpm for 2 h at 4°C using SW40Ti rotor in Optima L-80XP ultracentrifuge (Beckman Coulter). Absorbance at 254 nm was measured from lower to higher sucrose gradients using an ISCO gradient fractionation system and the optical density at 254 nm was continuously recorded with a Foxy JR Fractionator (Teledyne ISCO).
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bioRxiv - Immunology 2022Quote: ... eVLPs were purified by ultracentrifugation at 50,000 rpm (135,000 x g) for 2 hours at 4°C using a TLA100.3 rotor and a OptimaTM TLX ultracentrifuge (Beckman Coulter) on a 20% w/v sucrose cushion ...
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bioRxiv - Neuroscience 2023Quote: ... The top 11 mL and the bottom 2 mL of the gradient were transferred to separate 60 mL 45Ti ultracentrifuge tubes (Beckman), topped up with PBS and centrifuged at 100,000 x g for 70 min at 4°C ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was ultracentrifuged at 100,000 ×g at 4 °C for 2 h using an Optima XE-90 (Beckman Coulter) to collect EMVs ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Cell Biology 2023Quote: ... Each fraction was subjected to a washing step in ice-cold PBS at 100,000 × g at 4°C for 2 h using a SW28Ti rotor (Beckman Coulter). The pelleted EVs were resuspended in ice-cold PBS ...
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bioRxiv - Cell Biology 2023Quote: ... 5 mM Mg(OAc)2) and centrifuged for 20 min at 100,000 x g in a 70Ti rotor (Beckman Coulter). The resulting membrane pellet was resuspended in 1 ml rough microsome (RM ...
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bioRxiv - Physiology 2023Quote: ... Samples were ultracentrifuged at 100,000 rpm at 16°C for 2 hours (rotor: Beckman TLA-100; Centrifuge: Beckman Optima TLX). 100ul from the top of the tube was collected as VLDL fraction ...
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bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22,000 rpm for 2 hours at 4°C with OptimaTM L-90K Ultracentrifuge (Beckman Coulter) and stored at -80°C (never refrozen).
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bioRxiv - Biophysics 2023Quote: ... the supernatant of cell lysates was centrifuged to isolate the total membrane fraction at 24,000 rpm for 2 h in the 45Ti rotor using ultracentrifuge (Beckman-Coulter). Membrane resuspension in 50 mM Tris-HCl buffer (pH 8.0 ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was then overlaid onto a 2 ml 60% iodixanol cushion and spun at 100,000 x g (SW32 rotor, Beckman Coulter) for 18h ...
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bioRxiv - Neuroscience 2023Quote: ... This supernatant was next subjected to ultracentrifugation at 120,000 x g for 2 h at 4LJ in a SW 41 Ti Rotor Swinging-Bucket rotor (Beckman Coulter), and the pellets collected as small factors like exosome ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 200 µl 2% PBS-BSA and analyzed for mean fluorescence intensity (MFI) of ACE2 expression with CytoFLEX flow cytometer (Beckman). To determine the biological effects of the purinergic receptor P2X7 and NLRP3 inflammasome on viral entry ...
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bioRxiv - Biophysics 2023Quote: ... followed by layering the cleared lysate onto 40 mL of 2 M sucrose cushion and ultracentrifugation in Ti-45 rotor (Beckman) at 42,000 rpm for 2 hours ...
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bioRxiv - Neuroscience 2023Quote: ... Fraction LP2 was obtained after centrifugation of fraction LS1 for 2 h at 165,000 g in a SW40 Ti rotor (Beckman, US). Corresponding supernatant was collected as Fraction LS2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Virus particles were collected by centrifugation at 32,000rpm for 2 hours at 4°C using the Optima XE-90 ultracentrifuge (Beckman Coulter). Virus was aliquoted and stored at -80°C until further use ...
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bioRxiv - Plant Biology 2023Quote: ... and water to a final volume of either 2 μL for the acoustic liquid handling robot (Labcyte Echo 550, Beckman) or 5 μL for manual handling ...
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bioRxiv - Cancer Biology 2023Quote: ... The gradients were ultracentrifuged at 35,000 rpm for 2 hrs at 4°C using an SW41 Ti rotor (Beckman Coulter). The gradients were then displaced into a UA-6 continuous UV detector (Teledyne ISCO ...
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bioRxiv - Immunology 2023Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Developmental Biology 2023Quote: ... The resulting supernatant was subjected to ultracentrifugation at 120,000 × g at 4°C for 2 h (Optima Max TL, Beckman Coulter). Pellets were resuspended in 1 ml cold PBS and centrifuged at 120,000 × g at 4°C for 2 h ...
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bioRxiv - Immunology 2023Quote: The SARS-CoV-2 lentiviral pseudotyped neutralization assays were performed on integrated automation platforms consisting of a Biomek liquid handler from Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were ultracentrifuged at 4 °C and 150,000 ˗ g for 2 h (Optima L-80 XP Ultracentrifuge with Type 70.1 Ti Fixed-Angle Titanium Rotor (Beckman Coulter)) and supernatants were removed ...
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bioRxiv - Biochemistry 2024Quote: ... were added to the desired concentration before clarification by ultracentrifugation at 247,000 x (g) for 10 minutes at 2°C in a TLA100 (Beckman Coulter). Glucose oxidase (GmbH ...
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bioRxiv - Microbiology 2024Quote: ... passed through 0.45 µm filters and concentrated on a 20% sucrose cushion (24,000 rpm at 4 °C for 2 h with a SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2024Quote: ... and purified and concentrated by ultracentrifugation on a 20% sucrose cushion (24,000 rpm and 4°C for 2 hours with a SW32Ti or SW28 rotor (Beckman Coulter)) ...
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bioRxiv - Microbiology 2024Quote: ... The pellets from different centrifuge tubes were pooled and resuspended in 1 mL of 1X PBS and pelleted again by ultracentrifugation at 165,700 x g for 2 h at 4°C in a TLA-55 rotor (Beckman Coulter). The pellet was resuspended in 500 µL of 1X PBS and stored at -20°C until use ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant was ultracentrifuged at 100,000 g for 2 hours at 4°C with a 100 Ti rotor (Beckman Coulter) in a Beckman XL-90 ultracentrifuge ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge, Beckman 45 Ti rotor). Membrane pellets were resuspended to a total protein concentration of 3.5 mg/mL in ice cold 1 x PBS using the Bicinchoninic Acid (BCA ...
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bioRxiv - Immunology 2024Quote: ... and EVs were isolated by ultracentrifugation (UC) at 100,000 x g for 2 h using a SW40 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Bioengineering 2024Quote: ... The standard GE-caveospheres and the disrupted GE-caveospheres were centrifuged at 120,000g for 2 hours in an TLA-55 Fixed-Angle Rotor of Optima MAX-XP Ultracentrifuge (Beckman coulter) followed by imaging of fluorescence using a ChemiDoc MP Imaging System as above ...
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bioRxiv - Cancer Biology 2024Quote: ... The gradients were centrifuged at 40,000 rpm for 2 h at 4°C using a SW40 Ti rotor (Beckman Coulter). Eleven fractions of 500 µl each were collected and 200 µl and 50 µl were used for RNA and protein analysis ...
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bioRxiv - Cell Biology 2024Quote: ... The supernatant from the 10,000xg spin was layered on top of a 2 mL 60% iodixanol cushion and spun for 4 hours at 100,000xg (SW32 Ti rotor, Beckman Coulter). The bottom 3 mL of the cushion was obtained and layered at the bottom of a discontinuous gradient ...
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bioRxiv - Microbiology 2020Quote: ... were added into cells (1×106 cells/sample in 100 μL) and incubated for 60 min at 2-6°C and analyzed using flow cytometry (CytoFLEX, Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... thin-layer cell preparations for confocal microscopy of enriched small luteal cells and large luteal cells was performed using Cytofuge 2 (Beckman Counter). Cells were then fixed with 10% neutral buffered formalin at 4 °C for 30 min ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... We combined the 8 wells from column 2 of each plate and used the pooled culture to measure cell density on a Coulter Counter Z2 (Beckman Coulter). We spotted the entire volume of each of the other 88 wells on CSM-Arg (Sunrise Scientific ...
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bioRxiv - Evolutionary Biology 2021Quote: ... using a Beckman Coulter TLA100 rotor at 70,000 rpm at 4 C for 2 hours in a 13 mm x 51 mm polycarbonate ultracentrifuge tube (Beckman Coulter 349622). The sucrose solution was removed from the tube ...
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bioRxiv - Genomics 2020Quote: The ∼400 bp amplicon product produced using both Pool-1 and Pool-2 from the same clinical sample was combined and purified using a 1X concentration of Agencourt AMPure XP beads (Beckman Coulter); the final product was eluted in 30 µl of elution buffer (Qiagen ...
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bioRxiv - Cancer Biology 2020Quote: ... The donor-derived LT-HSC cell populations were sorted according to the gating strategy in Extended data Figure 2 using MoFlo Astrios Cell Sorter (Beckman Coulter). LT-HSC cells were directly sorted into 50μl RNA extraction buffer (ARCTURUS PicoPure RNA Isolation Kit (Applied Biosystems ...
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bioRxiv - Cancer Biology 2022Quote: ... 10 nM leptomycin b, vehicle control or library compounds (Supplementary Tables 2, 3) were added using an Echo liquid handler (Labcyte/Beckman Coulter). Cells were incubated for a further 24 h then fixed in 4% paraformaldehyde and stained with DAPI and Alexa Fluor 488 Phalloidin (ThermoFisher) ...
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bioRxiv - Microbiology 2022Quote: ... Washed pellets were resuspended in 2 mL 1x PBS and fluorescence was calculated using a LSR II cell analyzer (Beckman Coulter). Two biological replicates of 50,000 cell counts were analyzed with FlowJo ...
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bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Neuroscience 2020Quote: ... the homogenized solution was added onto a sucrose cushion (2 ml) in a 10 ml Ultra-Clear centrifuge tube (Beckman Coulter). Separation of nuclei from the tissue was done by centrifugation (26.500xg at 4°C for 1.5 h ...
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bioRxiv - Microbiology 2022Quote: ... filtered supernatant was underlaid with 20% sucrose PBS using a pipetting needle and centrifuged at 39 000 rpm for 2 h using an SW-41 rotor (Beckman Coulter). The supernatant and sucrose solution were carefully removed ...
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bioRxiv - Microbiology 2022Quote: ... The virus suspension was pelleted through a 40% sucrose cushion by centrifugation for 2 h at 40,000 rpm in a SW55 Ti rotor (Beckman, Fullerton, CA) at 4°C ...