Labshake search
Citations for Beckman :
251 - 300 of 725 citations for Mouse 20S Proteasome 20S PSM CLIA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... Uptake was terminated by rapid filtering of the cells into the kill solution during 20 s centrifugation at set maximum speed (microfuge B, Beckman, USA). For dark-inactivation experiments ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... and sonicated for 10 seconds with a QSONICA Q500 sonicator equipped with a micro tip and with the amplitude set to 20% prior to analysis in a Cytomics FC500 flow cytometer (Beckman Coulter).
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Expanding the toolbox for phycobiliprotein assembly: phycoerythrobilin biosynthesis in SynechocystisbioRxiv - Synthetic Biology 2023Quote: ... Ultracentrifugation was performed in a SW40 Ti swinging-bucket rotor at 130000 × g for 16 h at 20 °C (Optima L-80 XP, Beckman Coulter).
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Immunology 2023Quote: ... supernatants were concentrated by ultracentrifugation through a 20% sucrose cushion (44000 rpm, 45 min) using an Optima LE-80k ultracentrifuge (Beckman Coulter), and lysed in 2x SDS sample buffer (10% glycerol ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Molecular Biology 2024Quote: ... the fixed samples were subjected to sucrose cushion centrifugation (7,000x g for 20 min at 16 °C) using a swinging bucket rotor (Beckman, JS-7.5). Coverslips were recovered ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Biochemistry 2020Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 g for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Biochemistry 2020Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Biophysics 2021Quote: ... and grown overnight at 37°C for 4 h and harvested by centrifugation in a Beckman Avanti J25 centrifuge with a JA-20 rotor at 5,000 rpm (Beckman Coulter, Fullerton, CA). The cell pellet was resuspended in 10 mM Tris ...
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bioRxiv - Plant Biology 2021Quote: ... cesium chloride continuous density gradient in resuspension buffer and centrifuged at 34,000 m for 5 hours at 20 °C using an SW41 Ti rotor (Beckman Coulter, Brea, CA). The virus particles were observed in the lower of two pale white bands in the gradient ...
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bioRxiv - Microbiology 2020Quote: ... were pelleted through a 20% (wt/vol) sucrose cushion at 25,000 rpm for 2 h in an SW41 rotor (Beckman Coulter, Tokyo, Japan). Then ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample was centrifuged at a speed of 180000Xg (180000 RCF) in 7 mm x 20 mm polycarbonate centrifugation tubes (Beckman Coulter, USA) for at least 2h ...
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bioRxiv - Microbiology 2021Quote: ... unbound compound was separated from the astodrimer:virus mixture by pelleting the preincubated mixture through a 20% sucrose cushion (Beckman SW40 Ti rotor). The astodrimer sodium-containing supernatant was removed (i.e. ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate at a final concentration of 0.1% and then clarified by spinning at 14,000 rpm for 45 min (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 ml crude Ni-NTA column (GE healthcare ...
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bioRxiv - Microbiology 2020Quote: ... the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter, Indianapolis, IN) to remove unbound stain ...
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bioRxiv - Biochemistry 2019Quote: ... in iso-osmolar RB150/Mg2+ (containing a reduced level (2%) of glycerol) and 80µl were transferred to 7 × 20 mm polycarbonate tubes (Beckman Coulter, Brea CA), overlaid with 80µl of 35% ...
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bioRxiv - Genomics 2020Quote: ... Samples were eluted in 20 μL of elution buffer and 10 μL of each sample was pooled and concentrated to 20 μL using 0.7x AMPureXP beads (Beckman Coulter, Brea, CA). The final pooled sample was quantified using a Qubit Fluorometer and High Sensitivity DNA assay (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were harvested by centrifugation at 4,000 g for 20 minutes in an Avanti J-26 XPI centrifuge with a JLA 8.1000 rotor (Beckman Coulter, Brea, CA, USA).
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bioRxiv - Evolutionary Biology 2020Quote: ... Mitochondria were purified using a series of differential centrifugations: 20 min at 1500 g to pellet residual chloroplasts (8 °C, 3500 RPM, Beckman JA-12), 20 min at 20 000 g to pellet mitochondria (8 °C ...
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bioRxiv - Microbiology 2020Quote: ... Particles from media were concentrated by ultracentrifugation through a 20% (wt/wt) sucrose cushion (90 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Microbiology 2020Quote: ... followed by concentration of fixation-inactivated virions from media by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32 rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Immunology 2020Quote: ... Data were acquired with a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and results analyzed with Kaluza software (Beckman Coulter).
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bioRxiv - Immunology 2022Quote: ... Data were acquired with a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and results analyzed with Kaluza software (Beckman Coulter).
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was warmed to 37°C for few minutes until it turned cloudy following which it was centrifuged at 11,000 rpm at room temperature for 10 minutes (Beckman JA-20 rotor) to separate the soluble and detergent-enriched phases ...
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bioRxiv - Biochemistry 2022Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... The lysate was clarified by centrifugation at 5500 rpm for 20 min at 4°C (AllegraTM 21R Centrifuge, Beckman Coulter, CA, USA). The sample was then loaded into a HisTrap HP 5 mL column (Cytiva ...
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were analyzed with 2% agarose gel and transferred into 96-well PCR plate wells for clean-up with 20 µL of AMPure XP beads (Beckman Coulter #A63881) according to the manufacturer’s protocol ...
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HIV-2 Immature Particle Morphology Provides Insights into Gag Lattice Stability and Virus MaturationbioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in STE buffer and banded out over an OptiPrepTM step gradient (10, 20, 30% OptiPrepTM (v/v)) with a SW55 Ti rotor (Beckman Coulter, CA) at 45,000 rpm for 3 h ...
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bioRxiv - Biochemistry 2022Quote: ... VLPs were concentrated over 500 μL of 20% sucrose in PBS at 50,000 x g for 45 min (Beckman Coulter Optima TLX). Infections were assessed by western blot using the following antibodies ...
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bioRxiv - Immunology 2023Quote: ... Cells were examined by flow cytometry using a BD LSR Fortessa™ X-20 and data were analysed with the Kaluza software (Beckman Coulter). Dead cells were excluded from the analysis and the strategy described by Roos et al ...
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bioRxiv - Biochemistry 2023Quote: ... Triton-X was added to the lysate to a final concentration of 0.1% and clarified by spinning at 15,000 RCF at 4°C for 45 minutes (Beckman Coulter JA-20 rotor). The supernatant was loaded onto a 5 mL HisTrap™ FF crude column (GE Healthcare ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Biophysics 2023Quote: ... The virions were concentrated by ultracentrifugation through a 20% (w/v) sucrose cushion (2 h at 28,000 rpm in a SW32 rotor [Beckman Coulter Life Sciences]). The pellet was resuspended in PBS ...
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bioRxiv - Immunology 2023Quote: ... Data were acquired on a LSR Fortessa™ X-20 Flow cytometer (Becton Dickinson) and the results analyzed using Kaluza software (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... concentration of fixation-inactivated virions from media were spun down by ultracentrifugation through a 20% (wt/wt) sucrose cushion (120 min at 27,000 rpm in a Beckman SW32Ti rotor; Beckman Coulter Life Sciences). Pelleted particles were resuspended in PBS and stored in aliquots at −80°C.
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bioRxiv - Molecular Biology 2020Quote: ... and plasma was separated by centrifugation (2,000 × g, 20 min, 4 °C) in a Microfuge 20R (cat. no. B30149, Beckman Coulter, Brea, CA, USA). Plasma was transferred to new 1.5 mL microcentrifuge tubes and further clarified by centrifugation (10,000 × g ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with IPTG and grown overnight at 37 °C and harvested by centrifugation in a Beckman Avanti J25 centrifuge with a JA-20 rotor at 5000 rpm (Beckman Coulter, Fullerton, CA). The cell pellet was resuspended in 10 mM Tris ...
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bioRxiv - Microbiology 2020Quote: EV-depleted medium was obtained by overnight ultracentrifugation of DMEM supplemented with 20% FBS at 100,000xg in a Type 45 Ti rotor (Beckman Coulter, K-factor 1042.2). After ultracentrifugation ...
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bioRxiv - Biophysics 2022Quote: ... Fractions of EV-specific density were then pooled together and subsequently concentrated via ultracentrifugation (100,000 × g, 4 h, 4 °C) through a 20% w/v sucrose cushion in a SW41 rotor (Beckman Coulter, Fullerton, CA, USA). The resulting supernatant was discarded ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
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bioRxiv - Microbiology 2020Quote: ... The clarified supernatant was overlaid onto a 20% (w/v) sucrose cushion in Tris-NaCl-EDTA buffer and centrifuged at 134,000 g for 2 hours (Beckman Coulter 30 Ti rotor). The resulting viral pellet was suspended in VCM by repeat pipetting and aliquots stored in cryovials at minus 70°C until needed ...