Labshake search
Citations for Beckman :
251 - 300 of 1264 citations for 7 Chloromethyl 5H 1 3 thiazolo 3 2 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Systems Biology 2023Quote: ... Two libraries were prepared and sequenced: one with size-based selection for mRNA >4 kb using AMPure beads (Beckman) and a second library with no size selection.
-
bioRxiv - Molecular Biology 2022Quote: Procyclic form cell lines for depletion of either TbPH1 or TbKifX2A were induced at a density of 2×106 cells/ml with 1 µg/ml doxycycline and growth monitored daily with a Z2 Coulter Counter Analyzer (Beckman Coulter). Cells were diluted back to the initial cell density every day ...
-
bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
-
bioRxiv - Neuroscience 2024Quote: ... 20.000 rpm for 2 hours (Beckman). Collected viral particles were then suspended in DMEM and stored to –80°C ...
-
bioRxiv - Microbiology 2019Quote: ... the extracts were added on top of layers in LCM buffer with 45% and 5% glycerol and ultracentrifuged at 77 000 x g for 1 h at +4°C (Beckman Coulter SW41Ti rotor). Viral nucleocapsids were recovered from the bottom of the ultracentrifuge tube ...
-
bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
-
bioRxiv - Biophysics 2022Quote: ... 50 μl of each sample was centrifuged at a speed of 180000Xg (180000 RCF) in 7 mm x 20 mm polycarbonate centrifugation tubes (Beckman Coulter, USA) for at least 2h ...
-
bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
-
bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
-
bioRxiv - Immunology 2021Quote: ... the pellets were resuspended in cold PBS and transferred to one Ultra-Clear 13.2 mL tube (Beckman Coulter, ref 344059) and centrifuged again at 100,000g 4°C for 1 hour and 30 minutes ...
-
bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
-
Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).
-
bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
-
bioRxiv - Neuroscience 2023Quote: ... PI (1 μl/100 μl) was added 5 minutes before starting the reading on the flow cytometer CytoFlex S (Beckman Coulter, Brea, CA, USA). Results were shown and analyzed through CytExpert flow cytometry analysis software.
-
bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
-
bioRxiv - Genomics 2021Quote: ... After one wash with MACS buffer the percentages of IAV infected cells were determined with a MoFlo Astrios Cell Sorter (Beckman Coulter) and the uninfected gating control was defined using uninfected cells stained with both the primary and the secondary antibodies.
-
bioRxiv - Genomics 2021Quote: ... One milliliter of the precleared plasma or serum was transferred to a thick-wall polypropylene tube (Beckman Coulter, Brea, CA, USA) and centrifuged in an OptimaMAX (Beckman Coulter ...
-
bioRxiv - Cell Biology 2021Quote: ... GFP-positive cells were sorted to one cell per well into 96-well plates containing fresh culture medium using a MoFlo Cell Sorter (Beckman Coulter) and clones were gradually expanded to 6-well format over the course of several weeks ...
-
bioRxiv - Plant Biology 2022Quote: ... The supernatant was then recovered and centrifuged at 30,000 gav for one hour in the Ti45 rotor and in a Beckman L-80 ultracentrifuge (Beckman-Coulter, Mexico). The resulting pellet was re-suspended in 5 mL of modified CIB medium and centrifuged at 16,000 gav for 10 min in 5415R refrigerated centrifuge (Eppendorf ...
-
bioRxiv - Physiology 2023Quote: ... The compounds were dissolved at a concentration of 10 mM in DMSO and one batch of this library was distributed in a 384 well LDV microplate (LP-0200, Beckman coulter). For automated screening assay ...
-
bioRxiv - Bioengineering 2024Quote: ... Sonicated suspensions were ultracentrifuged at 58,000 x g for one hour using an Optima Max-XP Ultracentrifuge (Beckman Coulter; Brea, CA). The top 75% of the suspension was collected ...
-
bioRxiv - Immunology 2020Quote: ... Access SARS-CoV-2 IgG (Beckman Coulter), and LIAISON SARS-CoV-2 S1/S2 IgG (DiaSorin) ...
-
bioRxiv - Cancer Biology 2020Quote: ... and NEBNext® Multiplex Oligos for Illumina® (Index Primers Set 1 E7335S, Index Primers Set 2, E7500S) with Agencourt AMPure XP magnetic beads (Beckman coulter A63881) exactly as per manufacturer’s instructions ...
-
bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No ...
-
bioRxiv - Cell Biology 2022Quote: ... we used a CytoFLEX 5 flow cytometer (Beckman Coulter) and the CytExpert version 2.3 software ...
-
bioRxiv - Immunology 2023Quote: ... KIR2DL1/S1 (Beckman Coulter, clone EB6B: PE-Cy5-5) was performed at room temperature for 30 minutes in PBS (w/o Ca or Mg ...
-
bioRxiv - Genetics 2022Quote: ... Four μL from each successful reaction were combined into one 1.5 ml microfuge tube and the primers were removed using Agencourt AMPure™ XP beads (Beckman Coulter #A63881).
-
bioRxiv - Immunology 2023Quote: ... cells were activated with plate-bound anti-CD3 (500 ng/one well of 24-well culture plate; Beckman Coulter, REF# IM-1304) and soluble anti-CD28 (500 ng/ml ...
-
bioRxiv - Molecular Biology 2020Quote: ... bound antibodies were visualized using 100 µL of a 1:100 dilution of goat F(ab’)2 anti-human IgG coupled to phycoerythrin (Beckman Coulter, Brea, CA, USA) at 4°C for 30 min ...
-
bioRxiv - Biochemistry 2020Quote: Sedimentation velocity experiments were performed using a ProteomeLab XL-1 analytical ultracentrifuge with the use of an AN60-Ti rotor and Epon-2 channel centerpieces (Beckman Coulter, Brea, CA, USA). Protein samples were diluted with SANS buffer to concentrations yielding an absorbance of 0.5 at 280 nm as measured with an 8454 UV–Vis spectrophotometer (Agilent ...
-
bioRxiv - Neuroscience 2019Quote: ... and 1□mM DTT) before a centrifugation process of 2 h 20 min at 30,000□× g (Beckman J-25 centrifuge with a J13.1 rotor). The supernatant was carefully aspirated and the extracted nuclei in a form of a pellet were resuspended in a nuclear storage buffer (15% sucrose ...
-
bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
-
bioRxiv - Cell Biology 2022Quote: ... and the post-nuclear supernatant was loaded onto an 8-step discontinuous sucrose density gradient (HEPES-buffered 0.2–2 M sucrose) and centrifuged at 55,000 rpm for 2h at 4 °C using an MLS50 rotor (Beckman Coulter). Extracellular acidification rate (ECAR ...
-
bioRxiv - Biochemistry 2023Quote: ... lysosomes and mitochondria were enriched by centrifugation of the pooled PNS at 56,500 ×g for 7 min at 4 °C (Beckman-Coulter, 70 Ti fixed-angle rotor). The supernatant was removed ...
-
bioRxiv - Immunology 2019Quote: ... Receptor (R; Beckman Coulter, Z199, GL183, CD158e1/2) and KIR2DS4 (S4 ...
-
bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
-
bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
-
bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) and centrifuged for 2 hours and 15 minutes at 39000 rpm at 4°C (Beckman Coulter TLS55 rotor). Fractions corresponding to the large mitochondrial subunit were collected and subjected to buffer exchange (25 mM HEPES-KOH pH = 7.5 ...
-
bioRxiv - Immunology 2022Quote: CD27+ IgM- B cells cross-binding to SARS-CoV-1 and SARS-CoV-2 RBD were sorted from SARS-CoV-2 vaccinated SARS convalescents with MoFlo Astrios EQ Cell Sorter (Beckman Coulter). Briefly ...
-
bioRxiv - Evolutionary Biology 2021Quote: ... one half was purified using silica columns (MinElute PCR Purification Kit) and the other using 3x SPRI beads (Beckman Coulter™ Agencourt AMPure XP). Indexing PCR ...
-
bioRxiv - Microbiology 2021Quote: ... calibrating with 2-micron latex bead standard (Beckman Coulter) diluted in 18% BSW as the electrolyte ...
-
bioRxiv - Immunology 2021Quote: ... for 5 minutes (TEMP) and labeled with Viakrome 808 (Beckman Coulter) and fluorochrome-labeled antibodies against CD4 ...
-
bioRxiv - Immunology 2022Quote: ... anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter ...
-
bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
-
bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
-
bioRxiv - Immunology 2022Quote: ... Tagmented DNA was isolated using 2× SPRI Beads (Beckman Coulter) and amplified via PCR ...
-
bioRxiv - Neuroscience 2024Quote: ... the supernatant was transferred into a 5 mL polypropylene tube (Beckman Coulter), and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
-
bioRxiv - Biochemistry 2020Quote: ... Fragments were purified using 2 volumes AMPure XP beads (Beckman Coulter) and amplified for 8-10 cycles using NEBNext Multiplex Oligos ...
-
bioRxiv - Biochemistry 2021Quote: ... 6 mM 2-mercaptoethanol and centrifuged with a 70Ti (Beckman coulter) rotor at 40,000 rpm for 18 hours at 4°C ...
-
bioRxiv - Genomics 2022Quote: ... and purified with 2 rounds of AMPure XP beads (Beckman Coulter). Libraries were sequenced on an Illumina NextSeq 550 ...