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Citations for Beckman :
501 - 550 of 1264 citations for 7 Chloromethyl 5H 1 3 thiazolo 3 2 a pyrimidin 5 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... with 5% fetal bovine serum (FBS) and measured on flow cytometry with well-established compensated matrix (CytoFLEX, Beckman Coulter, Pasedena, CA). Analyses were performed using CytExpert Software (Beckman Coulter).
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bioRxiv - Molecular Biology 2020Quote: ... Samples were brought to a final volume of 300 μl if necessary with lysis buffer and loaded on a non-linear sucrose gradient prepared by layering different percentage of sucrose solutions (5%, 34% and 55%) into an Open-Top Thinwall Ultra-Clear Tube (Beckman, 344059). A 5x gradient buffer (100 mM HEPES pH 7.6 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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Preferred endocytosis of amyloid precursor protein from cholesterol-enriched lipid raft microdomainsbioRxiv - Neuroscience 2020Quote: ... 1.6 ml of 35% (w/v) sucrose and 5% (w/v) sucrose in MBS were layered in a 5.1 ml ultracentrifuge tube (Beckman Coulter, #326819) to form a discontinuous sucrose gradient ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Molecular Biology 2022Quote: ... The gradients were prepared using freeze-thaw method42 in Thinwall Ultra-Clear ultracentrifuge tubes (5 ml, 13×51 mm; Beckman-Coulter). To create the 50% sucrose cushion ...
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bioRxiv - Microbiology 2022Quote: The purified AdgI/AdgH complex sample was diluted to 0.5 mg/ml using the sample buffer containing 25 mM Tris-HCl pH 7.5 and 150 mM NaCl and subjected to the sedimentation velocity measurements using an Optima XL-I analytical ultracentrifuge (Beckman-Coulter). Data was analyzed with the SEDFIT and SEDPHAT programs33,34.
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Genetics 2023Quote: ... STR DNA fragments were amplified using a pair of specific primers with 5’ terminal parts of P5 and P7 Illumina adapters (Table S1) and purified by 100% (v/v) SPRIselect beads (Beckman Coulter). In the second step ...
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bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
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bioRxiv - Immunology 2023Quote: ... The live singlets were counted using DAPI 1:10 dilution by mixing 45 μl of cell suspension with 5 μl DAPI by gating on FSC-A vs FSC-H measuring average event/ μl on Cytoflex (Beckman Coulter). Subsequently ...
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bioRxiv - Microbiology 2024Quote: ... 0.2% Triton X-100 and 100 µg/mL chloramphenicol) and ultracentrifuged at 42500 rpm for 5 h in type 70 Ti rotor (Beckman Coulter) at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... ice-cold 5 mL 2.5 M sucrose was added to the bottom of an open-top plastic tube (Beckman-Coulter 344058), carefully topped up with ice-cold 5 mL 1.875 M sucrose ...
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bioRxiv - Plant Biology 2023Quote: ... for 15 minutes at 20°C followed by 5 minutes at 65°C for inactivation of the enzymes and purified with 1X AMPure XP beads (Beckman Coulter); 1D2 Adapters (ONT ...
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bioRxiv - Microbiology 2024Quote: ... incubated at 25°C and 5% CO2 (PF) or 37°C and 5% CO2 (BF) for 60 min and analyzed using a CytoFLEX flow cytometer (Beckman Coulter). Events (10,000 ...
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bioRxiv - Biochemistry 2024Quote: ... Supernatant was incubated in a boiling water bath for 5 min and centrifuged for 10 min at 40,000 rpm (Beckman Ti45 rotor). Supernatant was supplemented with ammonium sulfate (1 g per 6.1 ml) ...
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bioRxiv - Neuroscience 2024Quote: ... were loaded onto 10–50% continuous sucrose density gradients in polysome buffer (20 mM HEPES pH 7.4, 150 mM KCl, 5 mM MgCl2) (Gradient Master™, BioComp) and centrifuged in an SW40Ti swing-out rotor (Beckman Coulter) at 250,000 g for 2 h at 4°C ...
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bioRxiv - Genomics 2022Quote: ... HIV-1 p24 was stained with Anti-HIV-1 p24-PE (KC57) (Beckman Coulter) for 30 min on ice and washed again 3 times before measuring on the BD FACSCelesta™.
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bioRxiv - Microbiology 2021Quote: ... using anti-HIV-1 p24-PE antibody (#6604667, 1:1000 dilution) purchased from Beckman Coulter ...
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bioRxiv - Developmental Biology 2021Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Genomics 2023Quote: ... The samples were further purified using Ampure XP (1:1) (A63882, Beckman Coulter, US) and diluted in 10 µl elution buffer ...
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bioRxiv - Developmental Biology 2023Quote: ... cDNA was purified using a 1:1 volumetric ratio of Ampure Beads (Beckman Coulter) and eluted in 20 ml of water ...
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bioRxiv - Neuroscience 2021Quote: ... 2 mL of 20% (w/v) sucrose in DPBS (-Ca, -Mg) is prepared in ultracentrifuge tubes (Beckman Coulter, 344058) to which the divided EnvA- pseudotyped viral media is added before pelleting (20,000 RPM for 2 hours at 4°C) ...
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bioRxiv - Cell Biology 2019Quote: ... Sucrose gradients were subjected to ultracentrifugation (SW41 Ti 11E1698 rotor; Beckman at 260,000xg for 2 h at 4□°C) and fractionated by displacement using 60% sucrose/0.01% bromophenol blue on an ISCO Foxy fraction collector (35 s for each fraction ...
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bioRxiv - Cell Biology 2020Quote: ... Lysates were loaded on the top of the gradient and centrifuged at 260,000 × g and 4 °C for 2 h using SW 41 rotor (Beckman). Collection of polysomal fractions were done using Piston Gradient Fractionator (BioComp) ...
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Human Sex Matters: Y-linked lysine demethylase 5D drives accelerated male osteogenic differentiationbioRxiv - Cell Biology 2021Quote: ... Virus was transferred into conical tubes and spin at 20000rpm for 2 hours at 4°C in (Beckman TST2838) swinging bucket rotor ...
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bioRxiv - Biochemistry 2021Quote: ... 2 mM DTT) by being centrifuged at 73000 rpm for 45 minutes at 4°C (Beckman Coulter TL120.2 rotor). The mitoribosomal pellet was subsequently resuspended in ribosome resuspension buffer (25 mM HEPES-KOH pH = 7.5 ...
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bioRxiv - Biochemistry 2020Quote: ... The functional complex was recovered as a jelly-like pellet by centrifugation at 60,000 rpm at 25°C for 2 h in an ultracentrifuge (128,000 g in a TLA 120.2 Beckman rotor) and packed into a 1.9 mm rotor ...
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bioRxiv - Microbiology 2020Quote: ... and separated in potassium tartrate-glycerol gradient 10–35% for 2 hrs at 175,000 ×g (Beckman SW41 Ti rotor). Purified viral particles were suspended in 10 µg/ml BSA solution or 10 µg/ml CbAE-1 solution and incubated for 1 hr at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... and centrifuged at 24,000 rpm for 2 h at 4°C using a SW-Ti32 swinging bucket (Beckman Coulter). The upper layer was discarded ...
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bioRxiv - Cell Biology 2019Quote: ... MT seeds were diluted in BRB80 containing 2 μM taxol and centrifuged at 100,000 g (Optima TL100; Beckman Coulter) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: ... The supernatant was cleared again by 2 rounds of ultracentrifugation at 100,000 rpm (358,400 x g; Beckman TLA-120.2) for 10 minutes at 0°C ...
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bioRxiv - Cell Biology 2021Quote: ... Pellets were resuspended in 0.9% NaCl and spun at 110,000 × g for 2 hours at 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... The VLPs were concentrated by ultracentrifugation for 2 h at 31,000 rpm at 4 °C (Beckman SW-31Ti rotor), and then resuspended in 500 μL of Ex-Cell 405 medium ...
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bioRxiv - Molecular Biology 2021Quote: ... and the tube was centrifuged for 2 hours at 190,000 x g in a swing-out rotor (SW40 Ti; Beckman Coulter ...
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bioRxiv - Microbiology 2021Quote: ... and either aliquoted or concentrated by ultracentrifugation using 20% (w/v) sucrose cushions for 2 hours at 25,000 rpm in an SW41 rotor (Beckman).
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bioRxiv - Cell Biology 2022Quote: ... milk was prevented from cooling down and was processed within 20 minutes after collection by 2 rounds of centrifugation at 3,000xg for 10 minutes at 23°C (Beckman Coulter Allegra X-12R ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 2 ml (per fraction) of the supernatant and ultracentrifuged (Optima MAX-TL, Beckman Coulter) at 12,000 rpm (TLA55 rotor ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Molecular Biology 2023Quote: ... The gradients were centrifuged at 38,300 rpm for 2 hours and 45 min at 4 °C in a SW-40Ti (Beckman) rotor using an Optima XE-100 Ultracentrifuge (Beckman) ...
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bioRxiv - Plant Biology 2023Quote: ... Extract was then centrifuged at 40 000 rpm for 2 hours at 4°C using the 50Ti rotor (Beckman). A protocole previsouly used in the laboratory and described in Maclot et al ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000 xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Cancer Biology 2023Quote: ... RNA clean-up was performed by incubating RNA solution in 2 volumes of AMPure XP Beads (Beckman Coulter, A63881) for 2 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-1.5 μg of total protein was loaded onto a 10% to 50% sucrose gradient and centrifuged at 36,000 rpm for 2 hours at 4°C using an SW41 rotor (Beckman). Polysome profiles were visualized ...
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bioRxiv - Microbiology 2023Quote: ... 500 μ g of the lysate was loaded on the top of 15–50% sucrose gradient containing 100μ g/ml cycloheximide and the gradients were centrifuged at 36,000 rpm for 2 h at 4°C in SW41 rotor (Beckman). Density Gradient Fractionation System (ISCO ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Microbiology 2023Quote: ... The samples were centrifugated at 70000 rpm for 2 h at 4°C in an Optima TLX Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genetics 2023Quote: ... Fifteen A260 units of WCEs were layered on a pre-chilled 10-50% sucrose gradient and centrifuged at 40,000 rpm for 2 h at 4°C in a SW41Ti rotor (Beckman). Gradient fractions were continuously scanned at A260 using the BioComp Gradient Station and polysome to monosome (P/M ...