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Citations for Beckman :
251 - 300 of 457 citations for 3 Acetoxy 8 17 13E Labdadien 15 Oic Acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... was loaded onto the top of 6–8 M GuHCl gradients and centrifuged in a Beckman Optima L-90K Ultracentrifuge (Beckman SW40 rotor) at 40,000 rpm for 2.75 h 15°C ...
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cell Biology 2020Quote: ... The resulting supernatant containing AAV particles was added to the top of an iodixanol gradient (15%, 25%, 40%, 60% top to bottom) in an Optiseal tube (Beckman Coulter #361625). The gradient was then centrifuged using a Beckman Ti-70 rotor in a Beckman XL-90 ultracentrifuge at 67,000rpm for 70min ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... Final 15 mL of solution was mixed by inverting the tube 10x and carefully pipetted into 2 ultracentrifuge tubes (Beckman Coulter #344059) onto the isolation buffer cushion (5 mL ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cancer Biology 2021Quote: ... transferred to 96-well plates and incubated 15 min at room temperature with Agencourt AMPure XP magnetic beads (Beckman Coulter Cat# A63881) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Cell Biology 2022Quote: ... The plate was incubated for 15 min in darkness and measured in the flow cytometer FC 500 (Beckman-Coulter, Brea, CA, USA). This experiment was repeated 3 times on different days.
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bioRxiv - Neuroscience 2022Quote: ... Cell lysate was then loaded on an iodixanol gradient (60, 40, 25 and 15% iodixanol, ELITechGroup #1114542) in Beckman Quick-Seal Polyallomer tubes (Beckman-Coulter #342414) which were sealed and centrifuged in a Beckman-Coulter Optima XE-90 Ultracentrifuge at 16 °C and 69,000 rpm for 1 hour and 10 minutes ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a Beckman SW 40 rotor) for 3 hours at 10°C (18).
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bioRxiv - Immunology 2023Quote: ... After adding 450 μL of RBC lysis solution (QuantoBio Technology, Beijing, China) and 15 min incubation the samples were analysed using a DxFLEX flow cytometer (Beckman Coulter, USA). All data were analysed using FlowJo X (FlowJo ...
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bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
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bioRxiv - Microbiology 2024Quote: ... The supernatant was purified through a 15 to 45% (w/v) sucrose density gradient by ultracentrifugation at 160,000 × g (using a Beckman SW40 Ti rotor) for 2.5 h at 4 °C ...
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bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
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bioRxiv - Genomics 2020Quote: ... and separated by ultracentrifugation at 4°C for 3 hours (35300 rpm, Beckman, SW41 rotor) through a 5-50% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged at 150,000 × g for 3 hr (Beckman SW 55 Ti rotor) and subsequently ten fractions ...
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bioRxiv - Molecular Biology 2022Quote: ... Liberated adipocytes were measured using a 400 micrometer aperture on a Multisizer 3 (Beckman Coulter).
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bioRxiv - Neuroscience 2022Quote: ... Libraries were barcoded and pooled then underwent three 3 rounds of AMPure bead (Beckman-Coulter) cleanup at a 0.8:1 ratio of beads to library ...
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bioRxiv - Bioengineering 2022Quote: ... or by electrozone sensing (EZS) using the Multisizer 3 (Coulter Counter, Beckman Coulter, Brea, CA). Samples of 0.5–150 µL were used for the AccuSizer and Multisizer ...
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Phenotypic and genotypic adaptation of E. coli to thermal stress is contingent on genetic backgroundbioRxiv - Evolutionary Biology 2023Quote: ... and measuring cell density in volumetric mode on a Multisizer 3 Coulter Counter (Beckman Coulter). An isolate survived if its cell density measurements were maintained over the course of the test while allowing for fluctuations of +/− 1×106 cells.
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bioRxiv - Biophysics 2023Quote: ... precipitated by ultracentrifugation (35,000 rpm for 3 h at 4 ° C, Beckman-Coulter L90K, USA), and then redissolved in TNE/5 buffer.
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bioRxiv - Immunology 2024Quote: ... and analyzed by flow cytometry using a 3-laser Gallios flow cytometer (Beckman Coulter, USA). Cell debris ...
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bioRxiv - Immunology 2024Quote: ... at 100,000 × g for 3 hours and at 4 °C (Type 45 Ti, Beckman Coulter) to concentrate the exosomes ...
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bioRxiv - Cancer Biology 2024Quote: ... at 100,000 × g for 3 hours at 4°C (345776, Type 45 Ti, Beckman Coulter). Afterwards ...
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bioRxiv - Molecular Biology 2021Quote: ... the resin was spun down by centrifugation and nucleic acids were purified from supernatant with AMPure XP magnetic beads (Beckman Coulter). As a control ...
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bioRxiv - Cancer Biology 2022Quote: ... Nucleic acid size selection to enrich for fragment sizes between 200-500bp was conducted using SPRIselect (Beckman Coulter Life Sciences, B23318). Samples were pooled and sequenced on NextSeq2000 using TruSeq ChIP and Swift Bioscience Accel-NGS 2S Plus DNA Library Prep Kits and paired-end sequencing ...
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bioRxiv - Microbiology 2020Quote: ... CLP assembly reactions were incubated at 25°C for 30 min before loading onto 15-30% (wt/wt) sucrose gradients and spinning at 159,599 X g for 43 min at 4°C (Beckman Coulter TLS 55 rotor). Gradients were immediately fractionated and aliquots of each fraction were denatured by boiling in 1% SDS to ensure antibody access ...
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bioRxiv - Bioengineering 2020Quote: ... and the supernatant was centrifuged again at 26000 × g at 4 °C for 15 min using an Optima L-90K (Beckman Coulter, Brea, CA) ultracentrifuge with a 70-Ti fixed angle rotor (Beckman Coulter) ...
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bioRxiv - Biophysics 2021Quote: ... Different ratios of LUVs and cyt c (with the protein concentration kept at 5 μM) were incubated in the sample buffer at room temperature for 15 min in ultracentrifugal tubes (Beckman Coulter, Indianapolis, IN). The effective PG/cyt c molar ratio (R ...
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bioRxiv - Bioengineering 2021Quote: ... The tubes were then centrifuged at 1000 g for 15 minutes at 4°C using a Microfuge™ 22R (Beckman Coulter, Brea, CA), and the serum was then stored at -80°C ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 mM ATP and 1 mM PMSF) and centrifuged for 15 h at 4°C and 74 400 x g (Beckman SW 32.1 Ti). From the gradient ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the cell lysate was layered on top of a discontinuous OptiPrep® gradient (50 %, 35 %, 30 %, 20 %, and 15 %) and centrifuged at 135,000 g for 1 h at 4°C (Beckman L8-80M Ultracentrifuge, USA). The melanin pellet was recovered ...
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bioRxiv - Physiology 2023Quote: ... blood samples were centrifuged at 3000 RPM for 15 minutes at four °C in a refrigerated centrifuge (Beckman Coulter Allegra X-22 Series). Plasma samples were aliquoted and stored at -80°C ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 7h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Microbiology 2023Quote: ... cells were pelleted by centrifugation at 6,000 ×g at 4 °C for 15 min with ALLEGRA X-30R (Beckman Coulter, Brea, CA, USA). The supernatant was centrifuged at 15,000 ×g at 4 °C for 15 min with Sorvall LYNX4000 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Expression was continued for 16h and the cells harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000). The RING Zn-finger domains from both RING1B and PCGF4 subunits were cloned into a modified polycistronic expression vector (pET29a ...
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bioRxiv - Molecular Biology 2024Quote: ... To purify AAVs the entire lysate was loaded onto a 15-25-40-60% iodixanol (Serumwerk) gradient and centrifuged at 65’000 rpm (Beckman type 70 Ti rotor) for 2 h at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... Undissolved debris was removed by centrifugation at 12 k x g for 15 min using an Allegra X-30R centrifuge (Beckman Coulter Life Sciences). Protein assay was performed using BCA assay kit ...
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bioRxiv - Neuroscience 2024Quote: ... The final library was amplified using 15 cycles and the fragments ranging 200–1000 bp were size-selected using AMPure beads (Beckman Coulter, Cat.#A63881) (first round 0.5x beads ...
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bioRxiv - Microbiology 2020Quote: ... 3′ ligated RNA was cleaned by incubating with 2.5X volume of RNAClean XP beads (Beckman Coulter) and 1.5X volume of isopropanol for 15 min ...
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bioRxiv - Biophysics 2020Quote: ... After centrifugation at 30,000 rpm (4 ° C for 3 h) in the SW45Ti rotor (Beckman Instruments), eight 1.5-ml fractions were collected from the top of a gradient ...
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bioRxiv - Biochemistry 2020Quote: ... Gradients were resolved at 36,000 rpm for 3 hours at 4 °C in SW41 rotor (Beckman). Both separation and fractionation of gradients used a Biocomp Gradient Station (BioComp Instruments ...