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Citations for Beckman :
1 - 50 of 457 citations for 3 Acetoxy 8 17 13E Labdadien 15 Oic Acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: ... 20 mM Tris:HCl pH 7.5) in a zonal rotor (Ti 15, Beckman, 17 hours at 21,000 rpm). The peak containing pure 70S ribosomes was pelleted by centrifugation (20 hours at 35,000 rpm) ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (16) ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was collected and purified through 15-45% (w/v) sucrose density gradient by ultracentrifugation at 151,000 × g (using a 17 mL Beckman SW32.1 Ti rotor) for 3 hours at 10°C (18) ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biochemistry 2020Quote: ... disrupted with a tip sonicator at 1/3 power (8 MHz) for 3 × 30 seconds on ice and fractionated by ultracentrifugation (100’000 x g, 1H, Beckman TLA 120.2 rotor). The luminal fraction was concentrated to around 50 µL on a 3 kDa MWCO Amicon Ultra 0.5 mL centrifugal filter while membrane pellets were dissolved in 50 µL TBS with 2% SDS ...
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bioRxiv - Cancer Biology 2024Quote: ... Caspase 3 and 8 activity (EGFP+ cells) was analyzed using the CytoFLEX Flow Cytometer (Beckman Coulter) and Flowjo v10 BD (Becton Dickinson ...
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bioRxiv - Cell Biology 2023Quote: ... After centrifugation at 13,000 rpm (Beckman, JA-17) for 45 min ...
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bioRxiv - Cell Biology 2024Quote: ... After centrifugation at 15,000 rpm (Beckman, JA-17) for 30 min ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Populations were transferred twice a week by diluting 15% of the culture into 800 µL of fresh medium using a liquid-handling robot (Biomek NXP Span-8; Beckman Coulter). At each transfer ...
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bioRxiv - Cancer Biology 2020Quote: ... Tie2-PE and CD34-FITC antibodies (eBioscience 17-5821-81, 17-1171, 12-5987, 25-0411, 11-0341-82) by flow cytometry (Beckman Coulter CyAn ADP) or confocal microscopy using Confocal Microscope Zeiss LSM 880 with Airyscan.
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bioRxiv - Microbiology 2021Quote: ... 60% w/v) and centrifuged at 64,000 x g for 3 hours in A27-8×50 mL rotor (Beckman Coulter). The visible virus bands were collected ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Synthetic Biology 2021Quote: ... 8 °C (Beckman Coulter Optima MAX-XP ...
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bioRxiv - Genetics 2023Quote: ... the cell pellet was resuspended in PBS supplemented with 2 % FCS and nucleated cells were counted in methylene blue with 3 % acetic acid on a Vi-Cell XR cell viability counter (Beckman Coulter). 10 × 106 bone marrow cells were resuspended in 200 μl of PBS supplemented with 2 % FCS containing the following antibody solution ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Ribosome Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Ribosome Buffer containing 100 μg/mL cycloheximide in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
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bioRxiv - Synthetic Biology 2024Quote: ... the culture was collected into a 15 ml tube and spun for 15 min at 4000 xg using the Avanti J-15 R tabletop centrifuge (Beckman Coulter). Then ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 39,000 xg (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Biophysics 2022Quote: ... and the crude extract was centrifuged at 17000 g (JA-17 rotor, Beckman-Coulter) for 30 min at 4 °C ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... 5 ml of this mix was transferred to a 17-ml ultracentrifuge tube (Beckman) and overlayed with the following sucrose solutions ...
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bioRxiv - Immunology 2024Quote: ... Insoluble cellular debris was removed by centrifugation in a JA-17 rotor (Beckman Coulter) at 14,000 rpm for 15 minutes at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... 50%) in a 17 mL ultracentrifuge tube (Ultra-Clear, 16×102 mm, 344061, Beckman Coulter), and leaving overnight to allow for merging of the layers into a continuous gradient ...
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bioRxiv - Biochemistry 2020Quote: ... 15 min (Beckman J2-HC Centrifuge). Pellets were transferred to a 50 mL polypropylene Falcon tube and weighed ...
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bioRxiv - Genetics 2024Quote: ... The filtered medium was centrifuged at 19400 rpm and 17 °C for 2 h (Beckman Coulter). The pellet was suspended in 250 µL HBSS (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: The cell lysates obtained after sonication were centrifuged (16000 rpm, JA-17, 45 min, 4 °C, Beckman) to remove unlysed cells and other high molecular weight debris ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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bioRxiv - Biochemistry 2020Quote: ... for 15 min at 4°C (Beckman centrifuge J-20-XP ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Gradients were centrifuged at 49,100g for 16 to 17 hrs at 4°C in an SW32Ti rotor (Beckman). Gradients were fractionated using a Brandel (UV ...
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bioRxiv - Neuroscience 2021Quote: ... 17 cycles were used for cDNA pre-amplification followed by cDNA clean-up with SPRIselect reagent (B23318, Beckman Coulter) and cDNA quantification ...
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bioRxiv - Microbiology 2020Quote: ... The gradient was centrifuged for 17 h at 4°C and 100,000 rcf (23,700 rpm) using an SW 40 Ti rotor (Beckman Coulter), followed by manual fractionation into 20 590 μl fractions and measurement of the A260 nm of each fraction ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μL of extract was loaded on the 11 mL 5–20% sucrose gradient in the XB buffer and subjected to ultracentrifugation for 17 h at 197,000 ×g in the swinging bucket rotor SW41 Ti (Beckman). 0.5 mL fractions were collected and concentrated by using trichloroacetic acid precipitation with insulin as a carrier ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: CsCl linear gradients were prepared by overlaying 8.5 mL of 1.46 g/cm3 CsCl solution with 8.5 mL of 1.2 g/cm3 CsCl solution in 17 mL Ultra-Clear tubes (Beckman Coulter), which were then spun at a 45-degree angle and a speed of 20 rpm for 13.5 minutes using Gradient Master (BioComp).
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bioRxiv - Cancer Biology 2024Quote: ... and spinning at 12,535 g for 8 s (Beckman E centrifuge ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter; OptimaTM L-90K, Beckman Coulter). After the ultracentrifugation ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting solution was then centrifuged again at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was decanted ...
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bioRxiv - Plant Biology 2023Quote: ... The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant was then decanted and discarded ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Biochemistry 2023Quote: ... The reaction mixture was applied onto the top of the gradient solution and was centrifuged at 41,000 rpm at 4 °C for 17 hrs using an SW41 Ti rotor (Beckman Coulter). The gradient was fractionated using a Piston Gradient Fractionator (BioComp) ...
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bioRxiv - Microbiology 2024Quote: ... and fractionated by centrifugation at 32 000 rpm (130 000 × g) for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE and detected by western blotting.
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bioRxiv - Microbiology 2024Quote: ... 1 mM MgCl2) and fractionated by centrifugation at 130 000 × g for 17 h using an SW-41 rotor (Beckman). The gradient was divided into 20 fractions and proteins from each fraction were resolved on SDS-PAGE gels and detected by western blotting.
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bioRxiv - Microbiology 2023Quote: ... using a Biomek NXP Span-8 pipetting robot (Beckman Coulter), and pooled and concentrated on PCR purification columns (Analytik Jena) ...
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bioRxiv - Molecular Biology 2021Quote: ... mtEFG1 was subsequently removed from the mitoribosomes via a 10 – 40 % (w/v) sucrose gradient in 1x MB (17 h, 22500 rpm, SW 32 Ti rotor (Beckman Coulter), 4 °C) ...
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bioRxiv - Biochemistry 2021Quote: ... The UV-Visible spectrum of each sample was recorded for both tannic acid and gallic acid for both pH’s using a DU730 UV-Visible Spectrometer (Beckman Coulter, Pasadena, CA). The spectra were recorded from 250-400 nm with 0.2 nm data intervals ...