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Citations for Beckman :
201 - 250 of 1709 citations for Mouse Chemokine C X3 C Motif Receptor 1 CX3CR1 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... 4°C for 15 min using a JLA-9.1000 centrifuge rotor (Beckman). The pellet was resuspended in 70 mL buffer containing 50 mM Tris-HCl ...
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bioRxiv - Immunology 2023Quote: ... After adding 200 μL of optiLyse C Lysing solution (Beckman Coulter, France), the samples were analyzed with a Beckman Coulter flow cytometer using FlowJo X software ...
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bioRxiv - Immunology 2023Quote: ... After adding 600 μL of optiLyse C Lysing solution (Beckman Coulter, France), the samples were then fixed and permeabilised ...
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bioRxiv - Neuroscience 2023Quote: ... 4°C for 30 minutes in an OptimaTM MAX Ultracentrifuge (Beckman, Germany). Supernatant was collected and stored at -80°C ...
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bioRxiv - Immunology 2023Quote: ... After centrifugation at 100,000xg for 16 hat 4°C (Beckman Ti50.2 rotor), the density of each fraction was measured ...
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bioRxiv - Cell Biology 2023Quote: ... for 20 hours at 4 °C in an ultracentrifuge (LE-80K, Beckman). The centrifuged medium was diluted with serum-free Opti-MEM supplemented with penicillin/streptomycin to a final concentration of 10% BGS and filtered using the Stericup-VP Sterile Vacuum Filtration System with a 0.1 µM PES filter (Merck) ...
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bioRxiv - Evolutionary Biology 2023Quote: ... Final ultracentrifugation step at 100,000g/1hour/4°C (Beckman AirFuge CLS Ultracentrifuge) resulted in pellets which were resuspended in the same buffer (66) ...
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bioRxiv - Immunology 2023Quote: ... After adding 200 μL of optiLyse C Lysing solution (Beckman Coulter, France), the samples were fixed and permeabilised (BD Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... Gradients were centrifuged at 200,000xg for 16 h at 4°C (Beckman–Coulter Optima™ MAX–E centrifuge ...
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bioRxiv - Cell Biology 2023Quote: ... EVs were pelleted at 200,000xg for 60 min at 4°C (Beckman–Coulter Optima™ L–90K or Optima™ XPN–80 centrifuge ...
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bioRxiv - Neuroscience 2023Quote: ... and spun at 4°C in a fixed vertical rotor (VTi50, Beckman) at 242,000 g for 47 min to achieve subcellular fractionation.
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bioRxiv - Immunology 2019Quote: ... Cells were sorted for high expression of receptor ± introduced adaptor or CD8 co-receptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Biochemistry 2019Quote: ... SS-34 rotor) at 10,000 rpm at 4°C for 1 hours followed by 1 hour of ultracentrifugation (Beckman Optima™ LE, 80K, SW28 rotor) at 28,000 rpm (104000 x g ...
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bioRxiv - Biochemistry 2023Quote: ... Pellets were washed and gently resuspended in 1 ml of PBS and centrifuged at 50,000 × g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). The resulting pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2024Quote: ... Pellets were washed and gently resuspended in 1 mL of PBS and centrifuged at 50,000 x g for 1 h at 4°C (Beckman Optima MAX-E, TLA-55 rotor). Pellets were resuspended in 500 μl of PBS to obtain concentrated lentiviral stocks that were stored at −80°C in aliquots ...
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bioRxiv - Biochemistry 2024Quote: ... NaCl was then added to 1 M and the lysed solution was centrifuged at 20,000 g at 1°C for 45 minutes (Beckman Coulter J2-21, JA 20 rotor). Tween-20 was added to the supernatant to 0.1% (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
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bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Microbiology 2019Quote: ... The concentrated vesicles were washed twice with PBS by ultracentrifugation at 110,000xg at 4°C for 1 hour (Beckman Optma MAX-XP Ultracentrifuge, rotor TLA 120.2; Beckman Coulter Inc., USA). The final pellet (exosomes ...
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bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
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bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
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bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
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bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
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bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
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bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
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bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Genomics 2019Quote: ... the Promoter CHi-C libraries were purified with AMPure XP beads (Beckman Coulter). The concentration of the Promoter CHi-C libraries was determined by Bioanalyzer profiles (Agilent Technologies) ...
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bioRxiv - Genetics 2019Quote: ... The Hi-C libraries were purified with AMPure XP beads (Beckman Coulter, A63881) and sequenced using an Illumina sequencing platform.
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bioRxiv - Biochemistry 2019Quote: ... Induced cultures were grown at 18 °C overnight before harvesting by centrifugation (Beckman JLS 8.100 rotor ...
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bioRxiv - Biochemistry 2019Quote: ... Induced cultures were grown at 18 °C overnight before harvesting by centrifugation (Beckman JLS 8.100 rotor ...
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bioRxiv - Microbiology 2019Quote: ... 4°C in an ultracentrifuge (SW 40 Ti swing bucket rotor, Beckman Coulter). Gradient fractions were removed from the top in 1 mL aliquots ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were centrifugated at 1000 g for 5 min at 4°C (Beckman Coulter Microfuge 22R ...
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bioRxiv - Biochemistry 2020Quote: ... cells were harvested by centrifugation (6000 rpm, JA10, 20 min, 4 °C, Beckman). The pellet was re-suspended in 15-20 ml of 50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2020Quote: ... 4 °C for 38 min with MLA-50 rotor (Beckman Coulter, California, USA). The top layer and the subsequent 10 fractions (fraction 1-10 ...
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bioRxiv - Microbiology 2019Quote: ... 4°C in an ultracentrifuge (SW 40 Ti swing bucket rotor, Beckman Coulter). Gradient fractions were removed from the top in 1 mL aliquots ...
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bioRxiv - Biochemistry 2020Quote: ... centrifuged at 90,000 rpm for 10 min at 4°C (TLA120.1 Beckman Coulter), and then polymerized by incubation at 37°C for 30 mins ...
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bioRxiv - Microbiology 2021Quote: ... centrifuged at 42,000 rpm for 45 minutes at 4 °C (45Ti rotor; Beckman), and the resulting total membrane fraction was homogenized in TBS buffer containing 1.5% Lauryl-dimethylamine oxide (LDAO ...
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bioRxiv - Cell Biology 2022Quote: ... Gradients were ultracentrifuged at 192,000xg for 16 - 18 hours at 4°C (Beckman Coulter Optima XPN-80 with a SW40Ti rotor) ...
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bioRxiv - Genetics 2023Quote: ... the PCHi-C libraries were purified with AMPure XP beads (Beckman Coulter #A63881) and paired-end sequenced (HiSeq 1000 ...
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bioRxiv - Biochemistry 2023Quote: ... 4 °C in a Allegra X-15R centrifuge (Swinging bucket rotor, Beckman Coulter). The cell pellet was resuspended in 10 ml 20 mM Tris pH 7.5 and passed once through a French press at 120 MPa ...
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bioRxiv - Biophysics 2023Quote: ... alternated by centrifugation at 10°C and 6500 rpm (Beckman Coulter JLA 16.250). Enumeration of bacteria in the suspension was quantified with a Bürker-Türk counting chamber.
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bioRxiv - Biochemistry 2024Quote: ... at 4°C for 128 min using a SW 40Ti rotor (Beckman Coulter). After centrifugation ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Immunology 2019Quote: ... SSM cultures were grown for ∼22 h at 36°C with shaking at 180 rpm until the OD600 reached 0.5 on a 1 cm path width spectrophotometer (Beckman Coulter DU 530). The cultures were then diluted to 1 × 109/ml with SSM.
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bioRxiv - Biochemistry 2020Quote: ... to a final volume of 1 ml and then further centrifuged (120000 g, 4°C, 2 h) in a TLA-120.2 rotor (Beckman Coulter, Brea, CA). Pellets were washed twice with 4°C cold PBS and centrifuged (120000 g ...
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Micro RNAs are minor constituents of extracellular vesicles and are hardly delivered to target cellsbioRxiv - Cell Biology 2020Quote: ... Cat no UZ-PA-38.5-1) at 100,000 g at 4 °C for 2 h in a swinging-bucket rotor (Beckman Coulter; SW28 or SW32).