Labshake search
Citations for Beckman :
201 - 250 of 2041 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
-
bioRxiv - Cell Biology 2024Quote: ... The samples were then centrifuged for 1 h at 100,000 g and 4°C in a TLS 55 rotor (Beckman-Coulter) with the appropriate adapters (#358614) ...
-
bioRxiv - Microbiology 2024Quote: ... 20 mL of supernatant was gently layered on top of 10mL of 20% sucrose dissolved in PBS (4°C, sterile filtered) in an ultra-clear 1×3.5′ centrifuge tube (Beckman 344058). The tubes were spun down in a SW28 rotor (Beckman ...
-
bioRxiv - Microbiology 2024Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifuged at 100,000 rpm (543,000 × g) for 1 h at 4°C with a TLA110 rotor and an Optima MAX-TL ultracentrifuge (Beckman Coulter). Subsequently ...
-
bioRxiv - Biophysics 2024Quote: ... the clarified lysate underwent a further centrifugation step at 205,000xg for 1 hour at 4 °C (60Ti rotor, Beckman Coulter). The membrane pellet was then resuspended in the resuspension buffer ...
-
bioRxiv - Plant Biology 2020Quote: MP of pollen protoplasts was assessed by staining with 1 µM DiBAC4(3) for 5 min (Breygina et al. 2010) on Gallios flow cytometer (Beckman Coulter), analysis was performed with FlowJo software (TreeStar) ...
-
bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
-
bioRxiv - Developmental Biology 2024Quote: ... followed by ultracentrifugation at 120,000 × g at 4 °C for 2 hours (Beckman, TLA120.4 rotor). The resulting pellets were washed in cold PBS and underwent a second ultracentrifugation at 120,000 × g at 4 °C for 2 hours ...
-
bioRxiv - Cell Biology 2021Quote: ... About 9 ml interface that came from 3×SW28 tubes (25×89 mm, Beckman Coulter) was loaded in a SW41 tube (14×89 mm ...
-
bioRxiv - Genomics 2020Quote: ... followed by two size-selection steps with SPRIselect (1:0.6 and 1:0.2 sample vol. to beads vol.; Beckman Coulter, CA, USA). The libraries’ size distribution was determined by Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Bioengineering 2021Quote: ... containing 10% FBS and 1% PS and 50 nL of FDA-approved compounds (final concentration: 1 μM) using a pin tool (Biomek FX, Beckman Coulter). Cells were added to each well (1,500 cells/25 μL ...
-
bioRxiv - Microbiology 2020Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter, #A63880). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
-
bioRxiv - Genomics 2023Quote: ... and paramagnetic beads (1:1 beads/sample) using the same purification protocol as with AMPure XP beads (Beckman Coulter, no. B37419AB). The product purity was assessed via capillary electrophoresis on a QIAxcel instrument (Qiagen ...
-
bioRxiv - Immunology 2024Quote: ... The product of the reverse transcription reaction was purified with AMPure XP beads at a 1:1 bead to sample ratio (Beckman Coulter). Purified cDNA was split in half to amplify the heavy and light chains in separate reactions ...
-
bioRxiv - Immunology 2022Quote: ... following manufacturer’s instructions with the following p24 antibodies: HIV-1 p24 clone 28B7-APC (Medimabs) and HIV-1 p24 clone KC57-PE (Beckman Coulter). Acquisition was performed on the Fortessa LSR flow cytometer (BD Bioscience ...
-
bioRxiv - Microbiology 2024Quote: ... 2 µL per sample was used to pool the plates followed by concentrating using a 1:1 AMPure XP Beads clean up (Beckman Coulter). Beads were rinsed using two washes of 80% ethanol with 180-degree turns ...
-
bioRxiv - Cancer Biology 2023Quote: ... Filtered supernatants were concentrated by ultracentrifugation at 22.000 rpm for 2h at 4°C with OptimaTM L-90K ultracentrifuge (Beckman Coulter) and stored at −80°C (never refrozen).
-
bioRxiv - Biochemistry 2024Quote: ... A crude ribosome pellet was obtained by centrifugation for 2h at 200,000 × g at 4 °C (70Ti rotor, Beckman Coulter) and solubilized in HMA6/30 for 16h at 4°C ...
-
bioRxiv - Neuroscience 2023Quote: ... The resulting supernatant was filtered with a membrane pore size of 0.2 μm (Whatman) and centrifuged at 120,000 × g for 4 h at 4 °C in polycarbonate bottle assemblies (Beckman coulter, 355603) in a 70.1 Ti fixed angle rotor (Beckman coulter ...
-
bioRxiv - Biophysics 2022Quote: ... and the supernatant was subjected to ultracentrifugation to separate the membrane fraction (100,000g, 1 hour, 4°C using an Optima XE-90, Beckman Coulter centrifuge). Membranes were resuspended in lysis buffer supplemented with cOmplete EDTA-free protease inhibitors (Roche) ...
-
bioRxiv - Cell Biology 2022Quote: ... A new supernatant was collected in a prechilled 1.5 ml ultracentrifuge tube and centrifuged at 100000 g for 1 h at 4°C in a TLA-55 rotor (Beckman Coulter). The resulting pellet was resuspended in the same isotonic buffer containing antiproteases and was frozen at -80°C together with the 100000 g supernatant ...
-
bioRxiv - Systems Biology 2020Quote: ... each resultant fraction underwent separate ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778). In EV-enriched pooled fraction experiments ...
-
bioRxiv - Microbiology 2020Quote: ... Vesicles were obtained by centrifugation at 38,400 x g for 1 h at 4°C in an Avanti J-20XP centrifuge (Beckman Coulter, Inc). Pelleted vesicles were resuspended in 20 ml of 50 mM HEPES (pH 6.8 ...
-
bioRxiv - Microbiology 2020Quote: ... cells were fixed with 4% FA in PBS for 90 min at room temperature and immunostained for intracellular HIV-1 CA for 30 min at 4°C using KC57-FITC antibody (Beckman Coulter) diluted in 0.1% Triton X-100 ...
-
bioRxiv - Cell Biology 2023Quote: ... Homogenates were then transferred to a 50 ml tube and centrifuged for 10 mins at 1 000 × g at 4°C (Beckman Coulter). The resulting supernatant was then transferred to microfuge tubes for storage at -80°C as whole liver lysates or to fresh ultracentrifuge tubes for further processing.
-
bioRxiv - Cell Biology 2024Quote: ... plasma samples were thawed on ice and subjected to ultracentrifugation at 100,000 g for 1 hr or 18 hr at 4 °C (TLA-55 rotor; Optima MAX-MP Tabletop Ultracentrifuge, Beckman Coulter). Pellets were washed once in 1.0 ml PBS ...
-
bioRxiv - Microbiology 2024Quote: ... The final Hcp1-VLPs particles were pelletized by ultracentrifugation at 274,000 ×g at 4°C for 1 h in a SW41 Ti rotor (Beckman Coulter, Inc). The final pellet was resuspended in PBS pH 7.4.
-
bioRxiv - Bioengineering 2023Quote: ... The suspension was then ultracentrifuged (58,000 × g, 1 hr., 4 °C) in an Optima MAX-XP (Beckman Coulter, Indianapolis, IN, USA) to separate residual catalyst ...
-
bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
-
bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
-
Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
-
bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
-
bioRxiv - Developmental Biology 2024Quote: Cleared S10 fractions were adjusted to 500 μL with Subunit Lysis Buffer and overlaid onto 2 mL of a 1 M sucrose cushion prepared in Subunit Buffer in a 15×51 mm 3 mL thickwall polycarbonate ultracentrifuge tube (Beckman Coulter, 349622) on ice ...
-
bioRxiv - Biochemistry 2021Quote: ... cells were washed twice with PBS/1% BSA and resuspended in 500 µl PBS/1% BSA for analysis on a CytoFLEX S flow cytometer (Beckman Coulter, Australia). FITC was excited using a 488 nm laser and emission read at 525 nm using a 525/40 band-pass filter with a gain setting of 300 ...
-
bioRxiv - Neuroscience 2020Quote: ... Amplified cDNA samples were then cleaned up of short fragments with AMPure XP beads (1:1 ratio; Beckman Coulter, Brea, CA, USA) and checked for cDNA quality and concentration on a 2100 Bioanalyzer (Agilent Technologies ...
-
bioRxiv - Immunology 2020Quote: ... Plate was then incubated 1 h à 37 °C with 1 μg per well of alkaline phosphatase-coupled anti-Igκ or anti-IgM (Beckman Coulter) in PBS ...
-
bioRxiv - Genomics 2021Quote: ... We further purified the materials eluted from the columns by adding 150 ul (1:1) of AMPure beads RNAclean XP (Beckman coulter, A63987) and incubating for 5 min ...
-
bioRxiv - Microbiology 2024Quote: ... The PCR product was examined on gel electrophoresis and cleaned up by the AMpure XP beads (PCR product: bead 1:1 v:v, Beckman Coulter, Krefeld, Germany). The generated libraries were pooled with equal molar DNA ...
-
bioRxiv - Microbiology 2022Quote: ... The barcoded samples were pooled and purified using AMPure XP beads at a 1:1 ratio of sample:beads (Beckman Coulter; Brea, CA). The final libraries (350 – 800 ng DNA ...
-
bioRxiv - Microbiology 2024Quote: ... Illumina TruSeq LT adapters were ligated to the samples and cleaned up with a 1:1 bead ratio of AMPure XP followed by size selection using SPRIselect (Beckman Coulter, UK). The library was assessed for quality by Qubit and Agilent TapeStation ...
-
Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... CD45-PC7 Ab (clone J33, 1/15, Beckman Coulter), CD3-BV510 Ab (clone HIT3a ...
-
bioRxiv - Biochemistry 2021Quote: ... and 1 mM DTT) in a TLA100.3 rotor (Beckman) at 116,000 xg for 5 h ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... erythrocytes were lysed (1 ml of Versalyse, Beckman Coulter) and the lymphocytes fixed (25 μl of Fixative Solution ...
-
bioRxiv - Immunology 2020Quote: ... APC-A750 anti-CD3 (Beckman Coulter, clone UCHT-1), APC anti-CD4 (Beckman Coulter ...
-
bioRxiv - Neuroscience 2022Quote: ... 1 mM EDTA in centrifuge tubes (#344060, Beckman Coulter). The samples were spun in an ultracentrifuge (rotor ...
-
bioRxiv - Immunology 2022Quote: ... HIV-1 p24 core antigen KC57: 6604667 from Beckman Coulter and APC HIV-1 (p24 ...
-
bioRxiv - Immunology 2022Quote: ... ViaKrome 808 viability dye (1/50 dilution, Beckman Coulter) and CellBlox blocking buffer (1:30 dilution ...
-
bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
-
bioRxiv - Genomics 2023Quote: ... an additional 1× Agencourt AMPure XP bead (Beckman Coulter) clean-up was performed and eluted in 0.1× filtered TE ...
-
bioRxiv - Immunology 2024Quote: ... and APC NKG2A (1:100, clone Z199, Beckman Coulter) in PBS/0.1% BSA for 30 min at 4°C in the dark ...