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Citations for Beckman :
151 - 200 of 2041 citations for 2 Chloro 1 1 trifluoromethyl 1 3 4 9 tetrahydro 2H beta carbolin 2 yl ethan 1 one since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: ... Amplified PCR product was purified using a 1:1 concentration of AMPure XP beads (Beckman Coulter, Brea, CA, USA) and eluted in 30 μL of water ...
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bioRxiv - Genetics 2024Quote: ... Half of amplified DNA was purified with SPRI AMPure XP beads (sample to beads ratio 1:1, Beckman; A63881) and eluted in 15μl Buffer EB (QIAGEN) ...
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bioRxiv - Genomics 2024Quote: ... A 15-minute incubation at room temperature with a 1:1 volume ratio of AMPure XP beads (Beckman Coulter) was performed to concentrate samples ...
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bioRxiv - Plant Biology 2023Quote: ... followed by ultracentrifugation at 4°C for 2 h (38000 rpm, Beckman, SW41 rotor) through a 20-60% sucrose gradient (40 mM Tris-HCl pH 8.4 ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... at 4°C for 1.5-2h followed by 180,000 x g (Ti 70i rotor; Beckman Instruments, Inc., Fullerton, CA) at 4°C for 1.5-2h and resuspended in 1mL of phosphate buffered saline (PBS ...
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bioRxiv - Microbiology 2022Quote: ... which was spun in a ultracentrifuge at 30,000 x g for 2h at 4°C (SW 60 Ti rotor, Beckman) The pellet was resuspended in 20 μl of PBS for 1 h at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM PMSF buffer and subjected to ultracentrifugation at 36,000 rpm at 4°C for 2h using a SW41 rotor (Beckman) and 14 x 89 mm polypropylene tubes (Beckman ...
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bioRxiv - Microbiology 2022Quote: ... Libraries were purified and adapter dimers removed with Agencourt AMPure XP beads purification using a 1:1 library:beads ratio (Beckman Coulter). ChIP-seq libraries were sequenced on the Illumina NextSeq 500 system with a 20% phiX spike-in (Illumina ...
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bioRxiv - Immunology 2023Quote: ... the PCR plate was briefly centrifuged and 25 µl of room-temperature AMPure XP beads (1:1 ratio; Beckman Coulter) was added to each well and incubated for 8 minutes at room temperature ...
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bioRxiv - Genetics 2024Quote: ... and doubly size-selected by 0.4:1 ratio exclusion and 0.8:1 ratio inclusion for 300-500bp library size fragments (AMPure XP magnetic beads, Beckman Coulter). These pooled libraries underwent 2 × 250 bp sequencing (Illumina HiSeq 2500 ...
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bioRxiv - Systems Biology 2024Quote: ... Amplified 16S rDNA was purified using a 1:1 volume of Agencourt AmPure XP beads (Beckman-Colter, Brea, CA, USA). The purified products were pooled in equal amounts and analyzed using Illumina MiSeq sequencing ...
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bioRxiv - Immunology 2022Quote: ... and anti-HIV-1 p24 PE (Beckman coulter) were used ...
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bioRxiv - Neuroscience 2021Quote: ... in 1 ml polycarbonate ultracentrifuge tubes (Beckman Coulter) and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Immunology 2021Quote: ... CD3-PB (1:20; clone UCHT1; Beckman Coulter), CD4-PE-Cy7 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (J33, Beckman Coulter; 1:10) or CD3-APC ...
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bioRxiv - Cancer Biology 2024Quote: ... PE or FITC (UCHT1; Beckman Coulter; 1:10) were used to stain T-cells in co-culture assays ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were 1:1.3 AMPure XP (Beckman Coulter) purified and sent for sequencing ...
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bioRxiv - Molecular Biology 2024Quote: ... for 1 h in a Ti45 rotor (Beckman). The supernatant was loaded onto anti-FLAG G1 affinity resin (GenScript ...
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bioRxiv - Cell Biology 2020Quote: ... Crude mitochondria were loaded on top of the chilled gradient and separated at 100,000 Å∼ g for 1 h at 4°C (SW41 rotor; Beckman). Intact purified mitochondria were recovered from a brown band at around 16% Histodenz™ concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... The total bacterial lysate was centrifuged at 48,384 x g at 4 °C for 1 hr (Beckman JA-25.50 rotor), and the supernatant was applied to a 5 mL HisPur Ni-NTA resin (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... actins were cleared by highspeed centrifugation at 316,613 x g for 1 hour at 4°C in a TLA100 rotor (Beckman Coulter). Only the top 80% of the supernatant was collected to perform TIRF microscopy ...
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bioRxiv - Biochemistry 2020Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minutes 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). One aliquot of the eluted sample was resolved on SDS-PAGE ...
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kinesin recruitment by adapter SKIP on melanosomes is dynamically controlled by LC3B phosphorylationbioRxiv - Cell Biology 2021Quote: ... The post nuclear supernatant was collected and separated on a stepwise sucrose density gradient and centrifuged at 100,000 g for 1 hour at 4°C in a swing-out rotor (Beckman coulter). The sucrose density gradients comprised 0.25 ...
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bioRxiv - Biochemistry 2022Quote: ... and the debris and membranes were removed by ultracentrifugation at 205,000 g for 1 hr at 4 °C (rotor 45 Ti, Beckman Coulter). The supernatants were applied to IMAC with Ni2+-NTA-agarose resin (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... The sucrose gradient was centrifuged at 24,400 rcf for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter), and nuclei at the bottom of the gradient were resuspended in PBS ...
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bioRxiv - Plant Biology 2021Quote: ... The thylakoid membranes with different densities were analytically separated at 125,000 × g and 4°C for 1 h (SW 41 Ti, Beckman Coulter).
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bioRxiv - Microbiology 2020Quote: ... OBs were loaded on to a discontinuous 35–60% (w/w) sucrose gradient and centrifuged at 20000 rpm and 4°C for 1 hour in a SW41 rotor (Beckman). The band containing the OBs was removed ...
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bioRxiv - Neuroscience 2022Quote: ... 10 mM Tris-HCl) and centrifugated at 24,000 rpm for 1 h at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS ...
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bioRxiv - Immunology 2020Quote: ... Then the concentrated supernatant was ultracentrifuged at 110,000 g for 1 hour at 4°C (Beckman Coulter L100k, company name) to sediment the vesicular fraction into a pellet ...
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bioRxiv - Immunology 2021Quote: ... 1×106 thawed PBMCs were centrifuged (750×g for 5 minutes at 4°C) using a swinging bucket rotor (Beckman Coulter Avanti J-15RIVD with JS4.750 swinging bucket ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Digested samples were ultracentrifuged at 100,000x g for 1 h at 4 °C using a TLA-55 rotor (Beckman Coulter) to concentrate the insoluble ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...
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bioRxiv - Microbiology 2022Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minute 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). For SDS-PAGE analyses ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... loaded onto a discontinuous 0.75/1.0/2.33 M sucrose gradient and spin at 242,000 g for 1 h at 4 °C in a SW32Ti rotor (Beckman & Coutler). Growth cone fractions were collected at the interface load/0.75 M sucrose and non-growth cone fractions collected between 0.75/1.0 M sucrose and then analyzed by Western Blot.
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bioRxiv - Molecular Biology 2023Quote: ... Ribosomes were isolated by sucrose cushion ultracentrifugation at 100,000 rpm at 4°C for 1 h using TLA110 rotor (Beckman Coulter). RNAs in the ribosome pellet were isolated with TRIzol-LS (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2023Quote: ... It was then centrifuged again at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The final pellet containing the purified EV fraction was resuspended in 50 µl of 20 mM Tris-HCl pH 6.0 ...
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bioRxiv - Plant Biology 2023Quote: ... at 10,000 x g for 1 h at 4 ⁰C (JA-17 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). The supernatant from this step was decanted into the new tubes and centrifuged at 39,800 x g for 2 h at 4 ⁰C (JA-17 rotor ...
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Dopaminoceptive D1 and D2 neurons in ventral hippocampus arbitrate approach and avoidance in anxietybioRxiv - Neuroscience 2023Quote: ... 10 mM Tris-HCl) and centrifugated at 107,000 g for 1 hour at 4°C in a SW41Ti Swinging-Bucket Rotor (Beckman Coulter). Supernatant was discarded and nuclei pellets were re-suspended in 800 µL of PBS supplemented with 0.5% bovine serum albumin (BSA) ...
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bioRxiv - Plant Biology 2023Quote: ... The tubes were then centrifuged at 100,000 x g for 1 h at 4 ⁰C (TLA 55 rotor, Beckman Coulter; OptimaTM Max ultracentrifuge, Beckman Coulter). The pellets from the tubes were pooled together in 500 µl of 20 mM Tris-HCl pH 6.0 buffer and the volume was brought up to 1,500 µl using 20 mM Tris-HCl ...
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bioRxiv - Biophysics 2023Quote: ... Cell debris and unbroken cells were removed by centrifugation at 2,000 xg for 10 minutes and membranes were collected by ultracentrifugation at 186,000 xg for 1 hour at 4°C with a 45 Ti rotor (Beckman Coulter). The collected membrane was homogenized using a Dounce homogenizer in cold TBS buffer containing 1 mM PMSF ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was then transferred to polycarbonate tubes and centrifuged at 100,000 × g and 4°C for 1 h in a XL-90 ultracentrifuge (Beckman Coulter) ...
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bioRxiv - Cell Biology 2022Quote: ... This spin was repeated and the resulting supernatant was then centrifuged for 1 h at 75,000 g at 4°C (TLA-100.3 rotor or Type60 Ti rotor; Beckman Coulter, USA). The supernatant was aspirated and the membrane pellet gently resuspended in microsome buffer (10 mM HEPES/KOH pH 7.5. ...
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bioRxiv - Molecular Biology 2023Quote: ... for 45[min at 25°C and then subjected to a sucrose cushion ultracentrifugation at 100,000[rpm for 1[h at 4°C with Optima MAX-TL ultracentrifuge and TLA-110 rotor (Beckman Coulter). The RNA fragments in the resulting pellet were recovered using TRIzol reagent (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... and centrifuged at 100,000 × g for 1 h at 4 °C using a TLA-55 rotor in an Optima TLX Ultracentrifuge (Beckman Coulter). The first supernatant ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice with 200µl BD 1X Perm/Wash solution and stained 1h at 4°C with anti-HIV-1 p24 (RD1/PE, Beckman Coulter #6604667 ...