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Citations for Beckman :
151 - 200 of 2106 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2024Quote: ... CD138 and CD70 for CAR-T (see table of antibodies) and tumor identification and analyzed by flow cytometry using a CytoFLEX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo 10.10.0 software ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... gradient (6 ml 17 %, 6 ml 25 %, 5 ml 40 % and 5 ml 60 %) contained within an OptiSeal tube (Beckman,). Gradients were spun at 242 000 g at 16 °C and the viral fraction was harvested from the 40 % fraction with a 21-gauge needle ...
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bioRxiv - Cancer Biology 2023Quote: ... For cell apoptosis assay, Annexin V-PI apoptosis detection kit (Bioscience, A6030L) and Flow cytometry (Beckman, USA) were used according to the manufacturer’s instruction.
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bioRxiv - Systems Biology 2020Quote: ... and 1 mL of 5% (w/v) iodixanol solutions on top of each other in a 5 mL open top polyallomer tube (Beckman Coulter). Crude exosomes were resuspended in 200 µL PBS and overlaid on top of the gradient ...
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bioRxiv - Genomics 2019Quote: Human RNA libraries were individually cleaned using Agencourt AMPure XP beads (Beckman Coulter, #A63881) and a double-sided procedure (left 1×-right 0.6×) ...
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bioRxiv - Microbiology 2022Quote: ... human PBMC were incubated with monoclonal antibodies against CD19 (PE-Cy7; Beckman Coulter; IM3628), CD3 (PerCP ...
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bioRxiv - Cancer Biology 2020Quote: ... then genomic DNA was isolated from cells using the Agencourt DNAdvance DNA isolation kit (Beckman Coulter; Cat#: A48705). All LentiCRISPR target sites (except for those targeting Trp53 ...
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bioRxiv - Immunology 2021Quote: ... Stimulated cells were fixed and permeabilized with the Foxp3 staining kit (eBioscience) or IntraPrep Permeabilizaton Reagent (Beckman Coulter) and then stained with combinations of Foxp3 (FJK-16s ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Cell Biology 2022Quote: ... we used a CytoFLEX 5 flow cytometer (Beckman Coulter) and the CytExpert version 2.3 software ...
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bioRxiv - Neuroscience 2022Quote: ... anti-CD90 (IM1839U / Beckman Coulter / F15-42-1-5), anti-CD146 (A07483 / Beckman Coulter / TEA1-34) ...
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bioRxiv - Immunology 2023Quote: ... KIR2DL1/S1 (Beckman Coulter, clone EB6B: PE-Cy5-5) was performed at room temperature for 30 minutes in PBS (w/o Ca or Mg ...
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bioRxiv - Neuroscience 2023Quote: ... 5 ml of complete Neurobasal medium were added and centrifuged for 5 min at 335 x g (Beckman Coulter Allegra X-12R centrifuge). The complete Neurobasal medium consisted of ...
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bioRxiv - Cell Biology 2021Quote: ... for 5 minutes at 4°C with TLA100 rotor (Beckman). 100 µg cell lysate was brought to a final volume of 250 µl BRB80 supplemented with 1 mM GTP ...
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bioRxiv - Molecular Biology 2023Quote: ... 5 mM MgCl2) and spun in an SW41Ti rotor (Beckman) for 20 hours at 30,900 rpm ...
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bioRxiv - Cell Biology 2021Quote: ... platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin ...
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bioRxiv - Cell Biology 2021Quote: Platelets in PRP were labeled with a mouse anti-human monoclonal antibody CD61-PE (Beckman Coulter). Incubation was performed at room temperature for 10 minutes in the dark ...
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bioRxiv - Microbiology 2021Quote: ... and 10μl of PE-conjugated mouse-anti-human langerin Ab (Clone DCGM4; Beckman Coulter, Marseille, France). In other experiments ...
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bioRxiv - Microbiology 2019Quote: ... A portion of each sample was stained with Mouse-Anti Human CD15-PE (Beckman Coulter, USA) and run under BD Accuri C6 Flow Cytometer ...
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bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
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bioRxiv - Immunology 2021Quote: ... for 5 minutes (TEMP) and labeled with Viakrome 808 (Beckman Coulter) and fluorochrome-labeled antibodies against CD4 ...
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bioRxiv - Immunology 2022Quote: ... anti-NKG2A-APC (clone Z199; 5 μL; cat. # A60797) from Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
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bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Genomics 2019Quote: ... Total RNA extracted from mycelia cultured in Fries 3 was enriched for mRNA with the NEBNext® Oligo d(T) magnetic beads and concentrated with Agencourt® RNAclean® XP magnetic beads (Beckman Coulter, Inc. CA, USA). Direct RNA-seq and cDNA-PCR libraries were generated with ONT SQK-RNA001 and SQK-PCS108 library prep kits ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are then resuspended in PBS and incubated with APC-A700 anti-human CD56 (N901, #B92446 Beckman Coulter), APC-A750 anti-human CD90 (Thy-1/310 ...
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bioRxiv - Immunology 2022Quote: ... GEM-RT products were subsequently amplified using human BCR primers and cleaned up through SPRIselect beads (Beckman Coulter). Next ...
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bioRxiv - Bioengineering 2023Quote: ... or by staining with anti-human TCR mAbs (Vβ13.1-IMMU 222, Beckman Coulter for A2/NY A97L-TCR). Cell staining was performed at 4°C for 30min.
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
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bioRxiv - Neuroscience 2024Quote: ... the supernatant was transferred into a 5 mL polypropylene tube (Beckman Coulter), and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Cell Biology 2019Quote: Cell viability and Viable Cell Density (VCD) were determined using a Vi-CELL Cell Viability Analyzer (Vi-CELL XR cell counter, Beckman Coulter).
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bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
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bioRxiv - Cancer Biology 2024Quote: ... Platelet concentration was obtained by flow cytometry using an antibody against human CD41 labelled with PC7 (6607115, Beckman Coulter) and MP-count beads (6607115 ...
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bioRxiv - Molecular Biology 2019Quote: ... 500 mM sucrose) in a 5 × 44 mm micro centrifuge tube (Beckman Coulter) and sedimented by centrifugation (12000 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... Crosslinking reactions were loaded on 600µl 5∼35% glycerol gradients (Beckman 344090 tubes) in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors) ...
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bioRxiv - Cell Biology 2021Quote: ... Beads were centrifugated at 1000 g for 5 min at 4°C (Beckman Coulter Microfuge 22R ...
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bioRxiv - Cancer Biology 2020Quote: ... 3 and 5 and counted using a particle counter (Z1; Beckman Coulter, Inc.). Experiments were carried out in triplicate ...
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bioRxiv - Cell Biology 2023Quote: ... and then spun for 5 minutes in a chilled airfuge (Beckman Coulter, 340401) at around 80,000 rpm ...
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bioRxiv - Microbiology 2024Quote: ... gradient (25 – 39 % OptiPrep, 309600 x g, 5 h, SW60Ti rotor (Beckman Coulter). Purification of VLPs was realized accordingly ...
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bioRxiv - Molecular Biology 2024Quote: ... Discontinuous sucrose gradient was prepared in 5 ml ultracentrifuge tubes (Beckman Coulter, C14279) with 2 ml of 1.2 M sucrose solution (1.2 M sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were counted with a Vi-Cell Cell Viability Analyzer (Beckman Coulter).
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bioRxiv - Biochemistry 2022Quote: ... Cell numbers were counted using the Vi-Cell automated cell counter (Beckman) and proliferation calculated as fold change compared to the seeding density.
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bioRxiv - Developmental Biology 2023Quote: ... Cells were counted using Vi-CELL XR Cell Viability Analyzer (Beckman Coulter). Cells were routinely confirmed to be Mycoplasma-free by the MSKCC Antibody and Bioresource Core Facility and karyotypically normal by the MSKCC Molecular Cytogenetics Core ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were counted using a Vi-Cell XR Cell Counter (Beckman Coulter) and 2 X106 cells were resuspended in 1 mL lentivirus with 8 μg/mL polybrene in individual wells of a 12 well plate ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Immunology 2021Quote: ... as assessed by flow cytometry with Korman orange-conjugated mouse anti-human CD19 monoclonal antibody (mAb) (Beckman Coulter, CA, USA). The purified CD19+ B cells were cultured in the chemical-defined and serum-free X’VIVO 15 medium (Lonza ...
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bioRxiv - Immunology 2023Quote: Human TCR Vβ repertoire analysis was performed using antibodies against the most frequent TCR Vβ variants (covering about 70% normal human TCR Vβ repertoire, as reported by Beckman Coulter “Beta Mark TCR Vbeta Repertoire Kit” (cat.nr. ...