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Citations for Beckman :
51 - 100 of 2106 citations for Human Nuclear Factor Of Activated T Cells 5 NFAT5 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: T cell sub-population profiling studies on days 14 and 21 were analyzed using CD3-PC7 (Beckman Coulter, 6607100), CD4-APC-A700 (Beckman Coulter ...
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bioRxiv - Immunology 2024Quote: In functional assays proportion of infected CD4+ T cells was address by intracellular staining of p24 (KC57, Beckman Coulter) in combination with anti-CD3 ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II ...
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bioRxiv - Cell Biology 2020Quote: ... EVs were ultracentrifuged (Type 45 Ti rotor, k-Factor 133, Beckman Coulter) with 100000 g for 90 min 4°C ...
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bioRxiv - Immunology 2020Quote: ... Virus replication kinetics was monitored by SIV Gag p27 production in the supernatant using an SIV core antigen ELISA assay kit (Beckman Coulter, Miami, FL). To assess the properties of SHIV-Ag85B ...
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bioRxiv - Bioengineering 2022Quote: ... The frequency of proliferating T cells was determined after 96 hours by flow cytometry using a CytoFLEX II (Beckman Coulter). Zombie Green (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... TCRVβ14+ CD4+ naïve T cells from spleen and lymph nodes of TCR Tg mice using the MoFlo Astrios or MoFlo Astrios EQ (Beckman) at the Flow Cytometry Core in the UIC Research Resources Center (RRC) ...
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bioRxiv - Immunology 2023Quote: Flow cytometry analysis was performed at the end of the 7 day T cell progenitor differentiation stage using a CytoFLEX LX cytometer (Beckman), and data were compensated using single-stained controls in CytExpert v2.3 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5×10^6 cells counted by Multisizer 4e Coulter Counter (Beckman) were plated on a 60 mm plate and serum starved before analysis unless otherwise indicated ...
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bioRxiv - Microbiology 2021Quote: ... the percentages of anti-human IgG postive cells in GFP positive cells were then analyzed using flow cytometer (Cytoflex S, Beckman). LIVE/DEAD fixable aqua (Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2019Quote: ... 5′ cDNAs were purified using the Agencourt AMPure XP Kit (Beckman Coulter Genomics) and analysed by capillary electrophoresis before sequencing the single-end libraries using the Illumina NextSeq 500 system and a MID 150 Kit with 75 bp read length.
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bioRxiv - Cell Biology 2023Quote: Isolated nuclei were resuspended in TKMC buffer and nuclear size was measured with a CytoFlex (Beckman Coulter). To analyze protein levels ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... fixed angle Type 45 Ti rotor (k factor = 133; Beckman Coulter, Miami, FL, USA), at 4°C ...
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bioRxiv - Immunology 2022Quote: ... 25 μL of fetal bovine serum and 300 μl R2 Buffer were added to the cell suspension and transferred to DURAClone IF T Activation Tube (Beckman Coulter, USA), incubated in the dark for 45 minutes ...
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bioRxiv - Immunology 2020Quote: ... the cells were permeabilized (Beckman Coulter kit) and stained with p-(S465/S467)-Smad2/p- (S423/S425)/Smad3-Alexa 647 mAb Phosflow antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2024Quote: The Vi-cell reagent Kit (Beckman Coulter) was used according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Cells were pelleted at 200 x g 5 min in Allegra centrifuge (Beckman) and then resuspended in complete RPMI ...
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bioRxiv - Developmental Biology 2023Quote: The anti–human uncoupled primary antibodies used are listed: anti-human CD34 (Beckman, QBEnd/10), anti-human ACE (BB9 ...
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bioRxiv - Cell Biology 2023Quote: ... the post-nuclear supernatant was centrifuged for 30 min at 100,000 x g at 4°C using a S120AT2 rotor (Beckman Coulter). The resulting pellet containing the membranes was resuspended in SDS sample buffer (see below).
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bioRxiv - Immunology 2022Quote: ... anti-human TCR Vβ1 (Beckman Coulter), anti-human CD3 and CD8 antibodies (BD Bioscience).
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bioRxiv - Immunology 2024Quote: Cells were washed in DPBS plus 0.5% human serum albumin and incubated with anti-CD45-KrO (#B36294, Beckman Coulter), anti-CD3-APC (#IM2467 ...
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bioRxiv - Immunology 2019Quote: ... 5 µl of cDNA per cell was cleaned using Agencourt AMPure XP beads (Beckman Coulter) at a 1.0x ratio on a Hamilton STAR liquid handler (Hamilton Robotics) ...
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bioRxiv - Cancer Biology 2023Quote: ... After 5-7 days cells were counted using a Z1 Coulter Particle Counter (Beckman Coulter).
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bioRxiv - Genomics 2024Quote: ... After 5 days cells were harvested for genomic DNA purification using Agencourt DNAdvance (Beckman Coulter). Transfection of human primary hepatocytes were performed using Lipofectamine MessengerMAX Transfection Reagent (Thermo Fisher) ...
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bioRxiv - Cell Biology 2023Quote: ... The supernatant was centrifuged at 10,500 rpm (∼20,000 × g, k factor = 1,753) for 70 min (SW28 rotor, Beckman). Pelleted lEVs were resuspended in 13.5 ml of PBS (Lonza ...
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bioRxiv - Cell Biology 2023Quote: ... and sedimented again at 10,500 rpm (∼20,000 × g, k factor = 1,983) for 70 min (SW40 rotor, Beckman). The supernatant containing sEVs was centrifuged at 25,000 rpm (∼ 110,000 × g ...
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bioRxiv - Developmental Biology 2022Quote: ... and the optical density (OD) was measured at 450 nm with an ELISA reader (Beckman). The concentration of inflammatory factors was calculated according to the manufacturer’s instruction.
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bioRxiv - Genomics 2022Quote: ... PCR products with no hashtag libraries (spleen, mouse lung, and human lung cells) were combined and purified twice with 0.65× AmPure XP beads (Beckman Coulter) and eluted with 21 μL of nuclease-free water ...
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bioRxiv - Cancer Biology 2020Quote: ... The virally-transduced human CD34+ were sorted for GFP reporter expresssion on a MoFlo high-speed cell sorter (Beckman-Coulter) located at the New York Blood Center ...
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bioRxiv - Physiology 2020Quote: ... Total RNA was extracted from cell pellets using the Argencourt RNAdvance Cell V2 kit (Beckman) following the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... Total RNA was extracted from cell pellets using the Argencourt RNAdvance Cell V2 kit (Beckman) following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... reinhardtii cells were collected at 1,870 × g and 4°C for 5 min (JLA9.1000, Beckman Coulter). The cells were resuspended at 0.125 mg Chl/mL with disruption buffer containing 25 mM MES-NaOH (pH 6.5) ...
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bioRxiv - Immunology 2022Quote: ... A total of 5 × 105 living or fixed cells were analysed using a Cytoflex cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Molecular Biology 2019Quote: ... Gradients were centrifuged 18-20h at 192,000g in a SW40 rotor (k-factor 144.5) (Beckman Coulter, Brea, CA). For RNA extraction and small RNA library preparation ...
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bioRxiv - Cell Biology 2021Quote: ... 100K EVs were isolated by ultracentrifugation of 10,000xg supernatant at 100,000xg for 65 min in a SW40 rotor (k-factor 280.3) (Beckman-Coulter). EV-containing 100K ultracentrifugation pellets were resuspended in 20 μL PBS containing 0.2% BSA (cleared from aggregates by ultracentrifugation for 16-20 hrs at 100,000xg) ...
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Immunology 2024Quote: ... mApple-positive cells were sorted 5 days after infection on a Moflo XDP flow cytometer (Beckman Coulter) to obtain mApple-Mem-expressing Nalm6 cell lines.
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Developmental Biology 2021Quote: ... inhibin B and AMH/MIS by 2-site ELISA (Beckman Coulter and Beckman Coulter Gen II, respectively); and βHCG and AFP CMIA-ARCHTECT I 4000.The assays were performed as recommended by the manufacturers ...
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bioRxiv - Bioengineering 2021Quote: ... APC-labelled anti-human CD8 (Beckman Coulter, #IM2469), APC- or PacificBlue-labelled anti-human CD3 (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... anti-human CD4-FITC (clone 13B8.2, Beckman Coulter), anti-human CD8a-BV421 (clone RPA-T8 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... mRNA was purified from total RNA using poly- T oligo- attached magnetic beads (Beckman Coulter). Fragmentation was carried out using divalent cations under elevated temperatures in NEBNext First Strand Synthesis Reaction Buffer (5X) ...
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bioRxiv - Physiology 2019Quote: ... Agencourt AMPure XP PCR purification kit (5 ml Beckman Coulter Part No. A63880; 60 ml Beckman Coulter Part No. A63881) was used ...
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bioRxiv - Biochemistry 2020Quote: ... After 20 minutes the cells were collected by centrifugation (8,000 rpm, 5 minutes, JLA-16.25 Beckman Coulter rotor), dissolved in 0.5 mL of HEPES:Polymix buffer (5 mM Mg(OAc)2 ...
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bioRxiv - Molecular Biology 2020Quote: ... columns were spun at 100.000 g for 24 hours using a SW-28.1 rotor (k-factor: 276) in an optima XPN-80 ultracentrifuge (Beckman coulter), and subsequently fractions of 1 mL were collected ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 300,000×g at 4°C for 3 h in a TLA-110 fixed angle rotor (k-factor 13) (Beckman Coulter). The pellet was resuspended in 10 µL PBS of which 3 µL was adsorbed onto glow-discharged 2 nm carbon layer coated Quantifoil R1.2/1.3 400 mesh gold grids ...