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Citations for Beckman :
1851 - 1900 of 1974 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were detached with 0.25 % trypsin and subjected to flow cytometry analysis using CytoFLEX flow cytometer (Beckman Coulter, Brea, CA). Per sample ...
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bioRxiv - Cancer Biology 2023Quote: ... all co-cultured cells were harvested and analyzed for GFP and RFP fluorescence using CytoFLEX flow cytometer (Beckman Coulter, Brea, CA). Per sample ...
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bioRxiv - Biochemistry 2023Quote: ... the cell suspension was further diluted with 400 µl PBS and the stained cells were analyzed using the CytoFLEX flow cytometer (Beckman Coulter). Apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: Counting of CB CD34+ cells after 3 days of ex vivo co-culture was accurately obtained with flow-count fluorosphere beads (Beckman Coulter), in combination with the following antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentages of cells in the different stages of apoptosis were detected by using the CytoFLEX S flow cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates on a MoFlo Astrios cell sorter (Beckman Coulter), depositing one cell per well ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Molecular Biology 2023Quote: ... the measure of DNA content and the percentage of GFP positive cells were carried out with Gallios flow cytometer (Beckman Coulter) using the Kaluza Acquisition software (Beckman Coulter) ...
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bioRxiv - Synthetic Biology 2023Quote: EGFP transfected cells and HEK-293-loxP-GFP-RFP cells before and after PLP coincubation were measured with a CytoFLEX S cytometer (Beckman Coulter) using 488 nm laser with a 525/50 filter for measuring EGFP expression and a 561 nm laser with a 610/20 filter for measuring RFP ...
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bioRxiv - Neuroscience 2023Quote: ... Fluorescence Activated Cell Sorting was performed immediately after dissociation at the Zuckerman Institute Flow Cytometry platform using a MoFlo Astrios EQ (Beckman Coulter) sorter ...
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Bioengineering 2024Quote: ... we first measured a cell line population in PBS suspension using a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture) to obtain a cell volume distribution with the standard unit ...
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bioRxiv - Microbiology 2024Quote: ... Proportions of cells expressing either or both mCherry and GFP were analyzed by a CytoFLEX S flow cytometer (Beckman Coulter, USA). The criterion for assessing the extent of positive detection of each mCherry or GFP signal was set using mock-infected cells as a negative control.
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bioRxiv - Immunology 2024Quote: ... cells were washed twice with PBS and resuspended in 450 μL PBS for FACS sorting using the MoFlo Astrios Cell Sorter (Beckman Coulter). Cells were sorted into collection buffer comprising 150 μL of PBS and 20 μL of proteinase K.
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed once in FACS buffer and ligand binding was measured by flow cytometry on a Cytoflex instrument (Beckman Coulter). 104 events were collected per sample ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed once in FACS buffer and mAb binding was measured by flow cytometry on a Cytoflex instrument (Beckman Coulter). 104 events were collected per sample ...
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bioRxiv - Immunology 2024Quote: ... Cells were sorted for matched high expression of receptor plus introduced adaptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Immunology 2021Quote: ... the cells were washed and resuspended in RPMI with 10% of SFB and EDTA 0,05mM and submitted to cell sorting in Moflo Astrios® cytometer (Beckman Coulter®). The gate strategy and neutrophil yield are supplied in the supplementary figure 1.
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Genetics 2019Quote: ... We sorted 30 million cells for each screen into six bins based on fluorescence intensity of target genes using the Astrios EQ Sorter (Beckman Coulter B25982). To control for differences in staining efficiency for each cell ...
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Cell Biology 2020Quote: ... tSETD2-Flag plasmid was transfected into HEK293 Freestyle cells with FectoPRO transfection reagent (116-010) and cells were harvested 36-48 hours later at 5,000 rpm for 15 mins (Beckman JLA 8.1, 363563). The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Molecular Biology 2019Quote: ... the two initial washes were performed with PBS containing 0.5% Triton x-100 and cells were stained for 30 min with an α-p24 (gag) antibody (Coulter Clone KC57-RD1; Beckman Coulter). Data were acquired with a BD FACSVerse flow cytometer and analyzed using the FlowJo package (FlowJo LLC ...
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bioRxiv - Immunology 2019Quote: ... To identify KIR2DS1 negative donors, NK cells were stained with mAbs to KIR2DL1 (R&D, 143211) and KIR2DL1/S1 (Beckman Coulter, EB6) as described (Fauriat et al. ...
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bioRxiv - Microbiology 2021Quote: ... Vesicles were collected from cell-free supernatant culture by ultracentrifugation at 100000 × g (centrifuge Optima XPN-100 Beckman Coulter and rotor SW28) at 4 °C for 1.5 hours ...
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bioRxiv - Microbiology 2021Quote: ... cells were diluted 100-fold in PBS and green fluorescence (488 nm) and were measured using a cytoFLEX S (Beckman Coulter, USA). Data were analyzed using CytExpert (Beckman Coulter ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysate was then applied on a continuous 7-47% sucrose gradient (mol weight/volume) in ultra clear tubes (Beckman & Coulter, #331372) and centrifuged at 97,658 x g for 3 h at 4°C using a SW41Ti rotor (Beckman & Coulter ...
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bioRxiv - Cancer Biology 2020Quote: ... Twenty-four hours’ post nucleofection and subsequently every other day cells were analyzed using a CytoFLEX flow cytometer (Beckman Coulter, Krefeld, Germany).
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bioRxiv - Cell Biology 2021Quote: ... cells washed twice with PBS at each time point and whole cell fluorescence recorded with the Beckman Coulter Cytoflex flow cytometer (Beckman, Brea, CO). A total of 10,000 events counted per condition per sample using the fluorescein isothiocyanate (FITC-A ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Microbiology 2021Quote: ... Cells were counted at every passage (at 75-80% confluence) using a Beckman coulter Z1 particle counter (Beckman Coulter Canada, Mississauga, ON), blood cell counter vials (VWR ...
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bioRxiv - Microbiology 2020Quote: ... unstimulated PBMC cells (BenTech, Seattle, WA) were pelleted by centrifugation 228 x g for 8 min in an Allegra centrifuge (Beckman, Brea, CA). Pellets were resuspended in activation medium containing RPMI supplemented with 21 ng/mL human IL-2 (Peprotech ...
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bioRxiv - Molecular Biology 2022Quote: ... The supernatant was then passed through a cell sieve and underwent a 30 min ultracentrifugation (70 Ti rotor, Beckman Coulter, CA, USA) at 10,000 x g ...
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bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...
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bioRxiv - Biochemistry 2020Quote: tSETD2-FLAG was transfected into HEK 293 Freestyle cells with FectoPRO transfection reagent (Polyplus, 10118-444) and cells were harvested 48 hours later at 5K rpm for 15 mins (Beckman JLA 8.1).The pellet was suspended in lysis buffer (50 mM HEPES pH 7.5 ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Molecular Biology 2019Quote: ... Stained cells were washed twice in 500ul cold differentiation media and assayed on a 4 laser CytoFLEX S Flow Cytometer (Beckman Coulter, B75408). Data was processed in FlowJo V10 ...
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bioRxiv - Molecular Biology 2019Quote: The median values of the cell volumes of the population were obtained by a Coulter-Counter Z series device (Beckman Coulter, USA), as previously described [21] ...
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The Arabidopsis F-box protein FBW2 degrades AGO1 to avoid spurious loading of illegitimate small RNAbioRxiv - Cell Biology 2021Quote: ... The microsomal (Micro) and cytoplasmic (Cyto) fractions were collected by centrifugation of soluble cell extract in a TLA- 110 rotor (Beckman Coulter ultracentrifuge) at 100,000 x g for 30 minutes at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... virions were purified from the infected S2 cells by centrifugation at 8,000 RPM for 10 minutes at 4 °C using a Beckman GSA rotor (Beckman Coulter, Brea, California). The supernatant was then collected and centrifuged at 8,000 RPM for 30 minutes at 4 °C using a Beckman GSA rotor ...
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bioRxiv - Immunology 2021Quote: ... Cell debris was removed by centrifugation (45 min at 18,000 rpm, at 4°C; Avanti® J-26xp centrifuge (BECKMAN COULTER®)) ...
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bioRxiv - Cell Biology 2021Quote: ... cells were trypsinized and suspended in Krebs HEPES buffer and analysed using flow cytometry on a Cyan™ ADP analyser (Beckman Coulter). Analysis was performed using FlowJo software ...
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bioRxiv - Biochemistry 2021Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15), collecting the supernatant in a fresh sterile tube ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD127loCD25hi CD45RA+CD62Lhi cells and naïve CD4+ Tconvs were sorted from the CD25 negative fraction as live CD4+ CD45RA+CD62Lhi cells using a MoFlo® Astrios (Beckman Coulter). Cells were resuspended at 1×106 cells/ml in XH media (X-Vivo 15 (Lonza ...
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bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
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bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
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bioRxiv - Cancer Biology 2022Quote: ... The rate of cell apoptosis was performed by Cyto-FLEX S Flow Cytometer and was analyzed using FlowJo 7.6 software (Beckman Coulter, CA, USA).
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bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for the indicated number of days before trypsinization and counting on a ViCell™-XR Cell Viability Analyzer (Beckman Coulter). Fold-change in cell number was calculated relative to a baseline cell count obtained by counting cells from replicate plates that were collected on the day of media exchange ...
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bioRxiv - Immunology 2022Quote: ... 25 μL of fetal bovine serum and 300 μl R2 Buffer were added to the cell suspension and transferred to DURAClone IF T Activation Tube (Beckman Coulter, USA), incubated in the dark for 45 minutes ...