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Citations for Beckman :
1801 - 1850 of 1974 citations for Dengue Virus Serotype 2 Envelope Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2019Quote: Cells were stained for 30 min (minutes) with the anti-human monoclonal antibody CD11b-PE (IM2581U, Beckman Coulter, Brea, CA, USA), CD14-FITC (555397 ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed twice in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). Sorted cells with different scattering intensity were stained to identify the percentages of CD3+ CD4+ T/CD3+ CD8+ T ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through a 40 μm cell strainer and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). We used EasyFlow ...
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bioRxiv - Immunology 2021Quote: ... To sort specific subsets of C-LP mononuclear phagocytes cells were gated as above and sorted by MoFlo Astrios Sorter (Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... Cells were sorted for high expression of receptor ± introduced adaptor or CD8 co-receptor by fluorescence-activated cell sorting (FACS) (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Immunology 2019Quote: ... CHO ligand anchor HLA-A*02 cells were sorted for high expression of ligand anchor and HLA-A*02 using FACS (MoFlo Astrios, Beckman Coulter).
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Biochemistry 2019Quote: ... Haemoglobin and red cell indices (MCV, MCH and MCHC) were determined using an automated haematology analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2021Quote: Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter). Test sera were diluted 1:40 in growth medium and serially diluted ...
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bioRxiv - Cell Biology 2021Quote: ... BM analyses were terminal analyses at the time of tissue harvest and BM cellularity was determined using a ViCell automated cell counter (Beckman-Coulter).
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bioRxiv - Immunology 2020Quote: ... B- and NK cells within the lymphocyte population was performed by a whole blood staining lyse-no-wash protocol (Optilyse B, Beckman-Coulter) using six colour flow cytometry with fluorochrome-conjugated antibodies as listed in Supplementary Table 7 top ...
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bioRxiv - Immunology 2021Quote: ... cells were transferred to a 96 wells V-bottom plate and stained in PBS with ViaKrome 808 viability dye (Beckman Coulter). Next ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Immunology 2020Quote: Intracellular cytokine production by T cells was analyzed after stimulating PBMC or whole blood (fresh or frozen) 4h at 37°C in DurActive1 tubes (Beckman Coulter), which contain PMA ...
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bioRxiv - Immunology 2021Quote: ... Insoluble proteins and cell debris were removed by centrifugation (13,000 rpm, 30 minutes at 5°C, JA-30.50Ti rotor, Beckman, Palo Alto, CA). The soluble CuMVTT CP protein in clarified lysate was pelleted using saturated ammonium sulfate (1:1 ...
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bioRxiv - Cell Biology 2021Quote: ... the cell lysate after removal of cell debris at 850 g was first pelleted with an Optima MAX-XP Ultracentrifuge (with TLA-100 rotor, Beckman Coulter) at 35,000 rpm (47,265 g ...
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bioRxiv - Bioengineering 2021Quote: ... cells were resuspended in 100 µL PBSB and data were acquired on a CytoFLEX S flow cytometer (Beckman Coulter, Brea, CA) and analyzed by FlowJo software (FlowJo LLC ...
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bioRxiv - Immunology 2022Quote: ... and total RNA was isolated using Isospin cell and tissue RNA kit (Nippon Gene) or an RNAdvance v2 kit (Beckman Coulter) according to manufacturer instructions and quantified with an RNA HS Assay Kit (Thermo Fisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Single cells were flow sorted into single wells of 96-well plates using the MoFlo XDP100 cell sorter for ATTO550 positivity (Beckman Coulter) (University of Colorado Anschutz Medical Campus Cancer Center Flow Cytometry Core) ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... cells were seeded into pre-coated wells with anti-TCR pan-γδ clone IMMU510 antibody (5ug/ml) (Beckman Coulter, Krefeld, Germany) and IL-2 (100IU/ml) ...
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bioRxiv - Immunology 2022Quote: ... Cells were pelleted at 400xg for 5 min and washed with PBS twice before flow cytometry (Beckman Coulter CytoFLEX Flow Cytometer). Analysis of phagocytic ability was undertaken using FCS Express.
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bioRxiv - Synthetic Biology 2022Quote: ... in an optimized version of the Light Plate Apparatus (LPA).53 HEK293T cells were analyzed 24h after illumination using CytoFLEX S Flow Cytometer (Beckman Coulter) as described previously.53 For each sample ...
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bioRxiv - Molecular Biology 2023Quote: ... After incubation for 24 hours the cells were harvested and analyzed for fluorescence intensity with a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Equal optical density units from cleared cell lysates were loaded onto the gradient and centrifuged in a SW40 rotor (Beckman Coulter) for 5 hr ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were harvested after 3 days and counted using a Z2 Coulter Particle Count and Size Analyzer (Beckman Coulter, Brea, CA).
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bioRxiv - Microbiology 2024Quote: ... Stained cells were then washed once with PBS and ROS levels were analyzed using a CytoFLEX S flow cytometer (Beckman Coulter) with lasers at wavelengths of 405 nm (violet) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The FITC fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter). Relative expression levels of GPR1 mutants were represented according to the fluorescence signals.
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bioRxiv - Cancer Biology 2024Quote: Analysis of GALNT3 or fibronectin expression on human or mouse pancreatic cancer cells was performed using a flow cytometer (Gallios, Beckman Coulter) with appropriate antibodies ...
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bioRxiv - Cancer Biology 2024Quote: ... Conventional flow cytometry analysis was performed on 200 µL of whole blood cells using a lyse no wash protocol (Versalyse lysing solution, Beckman Coulter) or on 2 ×106 PBMC labeled and analyzed using a Fortessa cytometer (BD Biosciences ...
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bioRxiv - Genetics 2024Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates containing freezing buffer on a MoFlo Astrios cell sorter (Beckman Coulter). 30 nuclei of each were deposited per well ...
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bioRxiv - Genetics 2024Quote: ... and EGFP fluorescence was measured by FACS on either a SA3800 Spectral Cell Analyzer (Sony Biotechnology) or a CytoFLEX S Flow Cytometer (Beckman Coulter). Typically ...
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bioRxiv - Immunology 2024Quote: Individual CD24hi or CD24low Vγ1.1+ γδ T cells were isolated from Id3+/+ and Id3-/- mice by flow cytometry and deposited in wells of glass slides (Beckman-Coulter). Vγ1.1 and Vδ6.3 subunits were then amplified by RT-PCR using nested primers in single cells ...
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of the cell lysate in each well were transferred to a Proxi plate by a Beckman NX (Beckman Coulter). The plates were then promptly de-ionized (to prevent ESI spray instability ...
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bioRxiv - Synthetic Biology 2023Quote: EGFP transfected cells and HEK-293-loxP-GFP-RFP cells before and after PLP coincubation were measured with a CytoFLEX S cytometer (Beckman Coulter) using 488 nm laser with a 525/50 filter for measuring EGFP expression and a 561 nm laser with a 610/20 filter for measuring RFP ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting single nuclei of G1 phase (as determined by Hoechst and PI staining) were sorted into single wells of 96 wellplates on a MoFlo Astrios cell sorter (Beckman Coulter) and sorted at −80 degrees until further processing ...
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bioRxiv - Molecular Biology 2022Quote: Nuclei were isolated from 200 cotyledons of 5-day-old seedlings and purified using a two-layer Percoll gradient at 3000 g before staining with 0.5 μM DAPI and sorting by FACS according to their ploidy levels using a MoFlo Astrios EQ Cell Sorter (Beckman Culture) in PuraFlow sheath fluid (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... stained the cells by a Annexin V-FITC/PI Apoptosis Detection Kit (Vazyme, A211-01) and detected cells distribution using a Beckman Coulter Gallios (Beckman Coulter). The result was analyzed by Kaluza Analysis 2.1 (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... followed by flow cytometry analysis of CAp24 antigen expression on fixed and permeabilized cells labelled with KC57-fluorescein isothiocyanate (FITC) (Beckman Coulter).
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bioRxiv - Cancer Biology 2022Quote: ... The single muscle cell suspension was sorted by tdTomato+DAPI-(ECs) and tdTomato-DAPI- (non-ECs) using MoFlo Astrios (Beckman Coulter). The sorted cells were loaded into a 10x Genomics microfluidics chip and encapsulated with barcoded oligo-dT– containing gel beads using the 10X Genomics Chromium controller according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and PI staining) and single nuclei were sorted into wells of 96-well plates on a MoFlo Astrios cell sorter (Beckman Coulter). 96 well plates containing nuclei and freezing buffer were stored at –80°C until further processing ...
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bioRxiv - Microbiology 2023Quote: ... the cells were resuspended in 300 μL of FACS buffer and analyzed by using the CytoFlex cytometer (Beckman Coulter, Fremont, CA). Approximately 100,000 events were acquired for each sample ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates on a MoFlo Astrios cell sorter (Beckman Coulter), depositing one cell per well ...
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bioRxiv - Bioengineering 2023Quote: ... Viable Macrophages (Live+CD45+) and ODCs (Live+CD45-) were then sorted using a Beckman Mo-Flo Astrios cell sorter (Beckman Coulter) directly into 600 µL of RLT lysis buffer with 6 µL of β-mercaptoethanol ...
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bioRxiv - Neuroscience 2023Quote: ... CX3CR1-GFP and GFAP-CFP cells from adult male and female mice were submitted to FACS immediately after dissociation using a MoFlo Astrios EQ (Beckman Coulter) based on their fluorophore (GFP and CFP) ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with PBS before resuspension in MACS buffer for subsequent flow cytometric analysis using the CytoFLEX (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: Flow cytometric counts of the fresh cells were obtained by using a benchtop CytoFLEX S flow cytometer (Beckman Coulter, Brea, USA), equipped with the identical lasers and detectors as described previously [37] ...
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bioRxiv - Cancer Biology 2023Quote: ... The percentages of cells in the different stages of apoptosis were detected by using the CytoFLEX S flow cytometer (Beckman Coulter).