Labshake search
Citations for Beckman :
1801 - 1850 of 1886 citations for 6 Bromo 2 4 iodo phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Thylakoid membranes were extracted via ultracentrifugation at 24,000 rpm for 1 h and 20 min (Beckman Coulter SW 32 Ti rotor). Thylakoids were collected from the step gradient interphases with a Pasteur pipet ...
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bioRxiv - Microbiology 2022Quote: ... The PCR templates were purified using Agencourt AMPure XP (PCR product: AMPure XP beads = 1:0.8; Beckman Coulter, Brea, California, USA) before the second PCR ...
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bioRxiv - Molecular Biology 2022Quote: ... 2D10 cells were washed twice with 1 x phosphate buffered saline (PBS) and analyzed by flow cytometer (Epics Altra, BECKMAN COULTER) for the percentages of cells expressing GFP.
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bioRxiv - Molecular Biology 2022Quote: ... Samples were tagged using barcodes NB01 to NB05 of the Native Barcoding Expansion 1-12 PCR-free kit (EXP-NBD104, ONT) and purified using Agencourt AMPure XP beads (Beckman Coulter). The barcoded samples were quantified using a Qubit fluorometer and pooled equimolarly to reach 700 ng in 65 μL ...
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bioRxiv - Immunology 2022Quote: ... Samples are washed with 1X FACS buffer again to remove the PFA and finally re-suspended in 1 x FACS buffer for flow cytometry analysis using either Fortessa instrument (Becton Dickinson) or CytoFlex LX instrument (Beckman Coulter) fitted with an automated sampler accommodating 96-well plates ...
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bioRxiv - Molecular Biology 2022Quote: ... Samples were then enriched in DNA sizes between 200 and 600 bp (corresponding to 1 to 3 nucleosomes) using AMPure XP magnetic beads (Beckman-Coulter), following the manufacturer’s guidelines ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (23 ...
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bioRxiv - Genetics 2022Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase incubated for 4 hours at 37°C followed by inactivation with 1 μL of 10% SDS and purification using RNA Ampure XP beads (Beckman Coulter).
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bioRxiv - Cell Biology 2022Quote: Live Jurkat E6.1 cells were stained with Laurdan dye as detailed above and analyzed by flow cytometry using a Cytoflex S (Beckman Coulter). Cells were resuspended in HBSS without Ca2+ and Mg2+ (Gibco ...
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bioRxiv - Cell Biology 2019Quote: ... apoptotic and necrotic cells with YO-PRO-1 / PI in parallel with a Annexin-V / PI staining realized with the Annnexin-V-FITC when needed (Immunotech, Beckman-Coulter). All samples were measured and analyzed on a FACS Calibur 4C as previously described (73 ...
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bioRxiv - Immunology 2019Quote: ... cells were washed twice with permeabilization buffer and dissolved in FACS buffer (PBS, 1% BSA, 2mM EDTA) for analysis using a CytoFLEX S flow cytometer (Beckman Coulter).
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bioRxiv - Developmental Biology 2019Quote: ... with a final double (0.65 x −1 x bead volume) size selection step using a SPRIselect reagent kit (Beckman Coulter, B23318). The libraries were indexed (New England BioLabs ...
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bioRxiv - Developmental Biology 2020Quote: ... Exosome pellets were resuspended in phosphate-buffered saline (PBS) and centrifuged at 27300 rpm for 1 hour (SW60Ti rotor; Beckman Coulter). The exosome pellets were resuspended in PBS ...
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bioRxiv - Synthetic Biology 2021Quote: ... The complete NanoTrap was purified using rate-zonal centrifugation33 through a 1545% glycerol gradient in 1×TE + 10 mM MgCl2 in an SW 55 rotor (Beckman Coulter). Fractions were collected after a 1 hr centrifugation at 50 k rpm ...
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bioRxiv - Immunology 2019Quote: ... CECs were pre-incubated with human recombinant CCL5 (1-1000 ng/ml, R&D) and/or CD35 blocking antibody (5-20 μg/ml, clone J3D3, Beckman Coulter) for an hour prior to infection.
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bioRxiv - Neuroscience 2019Quote: ... Catalog L3224A) and lack of EtD-1 homodimer staining (L3224B) were selected and sorted by a MoFlo Astrios cell sorter (Beckman Coulter) into 500 µL filled into dissecting medium.
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through 40 μm Falcon™ Cell Strainers (Thermo Fischer Scientific, cat no. 08-771-1) and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). Matlab-based software for processing flow cytometry data was developed in-house by Yaron Antebi.
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bioRxiv - Cancer Biology 2021Quote: ... the 197 library compounds dissolved in DMSO or MilliQ (Supplementary Table 1) were added in duplicate using the Echo 550 dispenser (Beckman Coulter). Final drug concentrations ranged standard between 0.1 nM and 10 μM ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Genetics 2019Quote: ... The PCR products were examined on either 3% agarose gel by electrophoresis at 130 V for 1 hour or using capillary electrophoresis on an automated CEQ2000 system (Beckman-Coulter) as described previously (Raman et al. ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and permeabilized in PBS/1% BSA/0.1% saponin before staining with a FITC-conjugated anti-Cap24 (KC57-FITC, Beckman Coulter, France) for 1h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Cancer Biology 2024Quote: ... 200 μl of the PCR reaction (roughly corresponding to 1 μg of amplicon) was then purified using a double-sided selection with AMpure XP beads (Beckman Coulter). First ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Biophysics 2024Quote: ... cycled at 2 secs on / 2 secs off) and clarified by centrifugation at 8500 RPM for 1 hour using a fixed angle JA-20 rotor (Beckman Coulter) at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The pre-concentrated culture was centrifuged (10000 g, 10 min) in 1-L buckets (Avanti J-265 XP, Beckman Coulter, USA). The biomass reached a concentration of approximately 40 % (dry weight - DW ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Microbiology 2019Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987). Data were collected on Accuri C6 (BD Biosciences – U Mass ...
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Immunology 2019Quote: ... SSM cultures were grown for ∼22 h at 36°C with shaking at 180 rpm until the OD600 reached 0.5 on a 1 cm path width spectrophotometer (Beckman Coulter DU 530). The cultures were then diluted to 1 × 109/ml with SSM.
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bioRxiv - Genomics 2019Quote: ... Cells were suspended with 300 μl 1% FBS /HBSS (with 10 mM sodium butyrate) and subjected to FACS (Beckman Coulter MoFlo XDP) sorting for tdTomato positive cells ...
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Microbiology 2019Quote: ... Permeabilized cells were then incubated with 100 μl of 1:100 dilution of phycoerythrin-conjugated mouse anti-p24 mAb (KC57-RD1; Beckman Coulter, Inc.) for 30 min at 4 °C with intermittent mixing ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.