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Citations for Beckman :
1701 - 1750 of 1886 citations for 6 Bromo 2 4 iodo phenyl imidazo 1 2 a pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Cells were washed 1× with FACS buffer after staining and acquired on a CytoFLEX S cytometer (Beckman Coulter).
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bioRxiv - Systems Biology 2024Quote: The stained 1 in 3 cell solution was assessed by flow cytometry (CytoFlex S Flow Cytometer, Beckman Coulter) and the number of singlet (based on FSC-H vs FSC-A) ...
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bioRxiv - Immunology 2021Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
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bioRxiv - Genomics 2020Quote: ... The sequencing libraries were cleaned up using AMPure XP beads (1:1.8 sample vol. to beads vol.; Beckman Coulter). The size distribution of the libraries were determined by Bioanalyzer (Agilent Technologies) ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... diluted 5-fold in buffer A supplemented with 1 mM phenylmethane sulfonyl fluoride (PMSF) and centrifuged 40,000 rpm (45 Ti rotor, Beckman) for 60 min at 4°C to pellet vesicles ...
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bioRxiv - Biochemistry 2021Quote: ... and resuspended in 500 μL of 1% FBS/DPBS and transferred to polystyrene tubes for flow cytometric analysis (Beckman Coulter HyperCyAn ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were resuspended in p24 primary antibody solution (1:100 dilution of p24-FITC antibody clone KC57 (Beckman Coulter) diluted in perm/wash buffer ...
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bioRxiv - Neuroscience 2022Quote: ... The samples were separated through ultracentrifugation for 1 hr at 200,000 g (spin 3 at 49,000 r.p.m. in Beckman TLS-55 rotor). Top layers of supernatants were removed ...
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bioRxiv - Microbiology 2022Quote: ... Cells were then incubated with the fluorescently labeled anti-p24 antibody (Clone: KC57, Beckman Coulter, 6604667; 1/200 dilution).
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bioRxiv - Biochemistry 2022Quote: ... 1 mM DTT using a MLA-50 rotor in an Optima MAX-XP Ultracentrifuge (Beckman Coulter, Pasadena, California, USA) at 180.000 g for 5 h at 4 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... Amplicons from up to 3,072 libraries were pooled and purified with Ampure XP beads (0.8:1 ratio beads to product; Beckman Coulter). These steps were performed using Biomek FxP (Beckman Coulter) ...
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bioRxiv - Microbiology 2021Quote: ... buoyant density of 1.71 g ml−1) in polyallomer tubes before sealing and ultracentrifugation at 152,000 × g (50,000 rpm) in a MLN80 rotor (Beckman-Coulter) for 72 h at 25°C ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were refixed in 1% PFA/PBS and analyzed using a CyAn ADP Flow Cytometer (Beckman Coulter, Miami, FL). Data were collected using a four-decade log amplifier and stored in list mode for subsequent analysis using FlowJo Software (Tree Star ...
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bioRxiv - Genomics 2019Quote: ... All extractions from each respective fragment section were then pooled into two samples (pooled sample 5_27-10 and pooled sample 6_2-1) and re-purified using AMPure XP Beads (Agencourt, Beckman Coulter) adding 0.4 volume (V/V ...
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bioRxiv - Microbiology 2021Quote: ... cells were diluted in 50 mM filtered TrisHCl pH 7.5 to 106 mL-1 and analysed using a cytofluorimeter (CytoflexS, Beckman) with excitation and emission wavelength of 488 and 525 nm respectively ...
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bioRxiv - Molecular Biology 2019Quote: After depletion of the gene of interest samples were purified using a 1 X volume XP beads (Beckman Coulter), quantified and subsequently subjected to 75-bp-single-end sequencing on the Illumina Nextseq 500 platform.
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bioRxiv - Neuroscience 2019Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by aspirating the interface between 40-60% iodixanol with a syringe ...
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bioRxiv - Immunology 2020Quote: ... 10 mM Tris-HCl, pH 8.1), chromatin was eluted (1% SDS, 0.1M NaHCO3)and recovered with Agencourt AMPure XP beads (Beckman Coulter). Bound chromatin and input DNA were treated with RNase H (New England Biolabs ...
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bioRxiv - Cell Biology 2019Quote: ... F-actin was pelleted by ultracentrifugation for 1 h at room temperature at 45,000 rpm (Beckman TLA-55 rotor) and re-suspended in ice cold G-buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 250 µl FACS buffer with 1 µl DAPI and analyzed with a Gallios flow cytometer (Beckman Coulter)
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (5 mg/ml) and floated for 2h at 55,000 rpm in a TLS-55 rotor (Beckman) at 25 °C through a sucrose gradient ...
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bioRxiv - Microbiology 2022Quote: ... 1 mM dithiothreitol and 100 μg/ml cycloheximide) and spun for 1 h at 100,000 rpm using TLA-110 rotor (Beckman) at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... the cross-linked material was loaded onto glycerol-tartrate gradients as described elsewhere 53 and centrifuged for 1 h at 25,000 rpm in an SW-40 rotor (Beckman), with brakes set at the slowest possible deceleration ...
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bioRxiv - Neuroscience 2022Quote: ... Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman). The AAV particles were collected by collecting the interface between 40 and 60% iodixanol with a syringe ...
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bioRxiv - Evolutionary Biology 2024Quote: ... DNA was enriched in samples with low DNA concentration (1-60 ng/μl) using AMPureXP Beads (SPRIselect, Beckman Coulter) in a 1:1 ratio ...
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bioRxiv - Developmental Biology 2023Quote: ... Double size selection to remove primer dimers and fragments exceeding 1 kb was performed using SPRIselect beads (Beckman Coulter). Another quality control was performed with High Sensitivity DNA assay using the Fragment Analyzer (Agilent) ...
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bioRxiv - Cell Biology 2024Quote: ... mTurquoisehigh cells were sorted into 96-well plates at 1 cell/well using a MoFlo Cell Sorter (Beckman Coulter). For cells transfected with sgRNA/Puro plasmids ...
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Small Molecule Screen Identifies Pyrimethamine as an Inhibitor of NRF2-driven Esophageal HyperplasiabioRxiv - Cancer Biology 2022Quote: ... Individual compounds (1 µM in 0.1% DMSO) were added to each well using a Biomek NX workstation (Beckman-Coulter)(1 well per compound) ...
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bioRxiv - Immunology 2023Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching mid-log phase OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
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bioRxiv - Developmental Biology 2023Quote: ... Size selection and purification was achieved by double-sided (0.65 × −1× volume) using SPRIselect magnetic beads (Beckman Coulter, B23318). Concentrations were measured using the Qubit HS dsDNA assay ...
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bioRxiv - Genomics 2024Quote: ... They were transduced via spinfection at 1000 rpm for 1 hour at 30 °C (Beckman Coulter, Allegra X-14R), followed by overnight incubation ...
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bioRxiv - Microbiology 2024Quote: ... For p24 staining (RD1-conjugated mouse anti-p24 clone KC57, cat# 6604667, 1:100 dilution, Beckman Coulter, Brea, CA), cells were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and resuspended in PBS 1% FBS 2mM EDTA and run on a Cytoflex LX (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... Amplicons were visualized on a 1% agarose gel and purified with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA). The concentration of purified amplicons was quantified using the Qubit High Sensitivity dsDNA kit and a Qubit Fluorometer (Invitrogen) ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1×MMR final were manually prepared in pre-chilled 14 mL ultra-clear centrifuge tubes (Beckman Coulter, #344060) in the following order from the bottom to the top of the tube ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Microbiology 2019Quote: ... and centrifuged at 40,008 × g for 1 hour at 4ºC in an Beckman L-100 XP ultracentrifuge with the SW 41 Ti swing rotor (Beckman Coulter). Following centrifugation ...
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bioRxiv - Biochemistry 2019Quote: ... the cells were dissolved in the same buffer as for TtBac-WT 1-123 and spun for 30 min at 9k rpm in a Ti45 rotor (Beckman). The pellet was dissolved in the same buffer and from here onwards the same protocol as for TtBac-WT 1-123 was used.
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bioRxiv - Bioengineering 2021Quote: ... Cultures were grown in a MTP-48-BOH 1 FlowerPlate in a BioLector Pro (both Beckman Coulter Life Sciences, USA) at 1400 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Systems Biology 2020Quote: ... GFP productivity was determined by measuring the GFP signal (FL-1 channel) of cells by flow cytometry (Gallios, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA (corresponding to a refractive index of 1.3700) and loaded into OptiSeal polypropylene centrifuge tubes (Beckman Coulter, 362185). Centrifugation was performed in a near-vertical NVT-90 rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g 1 h).
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bioRxiv - Neuroscience 2022Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g, 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...