Labshake search
Citations for Beckman :
1601 - 1650 of 1681 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... CD4+CD127loCD25hi CD45RA+CD62Lhi cells and naïve CD4+ Tconvs were sorted from the CD25 negative fraction as live CD4+ CD45RA+CD62Lhi cells using a MoFlo® Astrios (Beckman Coulter). Cells were resuspended at 1×106 cells/ml in XH media (X-Vivo 15 (Lonza ...
-
bioRxiv - Cancer Biology 2022Quote: ... The supernatants were further centrifuged at 10,000 × g for 30 min at 4°C to remove remaining cell debris and at 100,000 × g (Type 90 Ti Rotor, Beckman, Brea, CA, USA) overnight at 4°C to remove exosomes ...
-
bioRxiv - Biochemistry 2022Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
-
bioRxiv - Cancer Biology 2022Quote: ... The rate of cell apoptosis was performed by Cyto-FLEX S Flow Cytometer and was analyzed using FlowJo 7.6 software (Beckman Coulter, CA, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... Cells were cultured for the indicated number of days before trypsinization and counting on a ViCell™-XR Cell Viability Analyzer (Beckman Coulter). Fold-change in cell number was calculated relative to a baseline cell count obtained by counting cells from replicate plates that were collected on the day of media exchange ...
-
bioRxiv - Immunology 2022Quote: ... 25 μL of fetal bovine serum and 300 μl R2 Buffer were added to the cell suspension and transferred to DURAClone IF T Activation Tube (Beckman Coulter, USA), incubated in the dark for 45 minutes ...
-
bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
-
bioRxiv - Bioengineering 2024Quote: Exosomes from each cell line were isolated from a conditioned medium based on differential ultracentrifugation (Optima™ XE-100, Beckman Coulter, USA) [57] ...
-
bioRxiv - Immunology 2023Quote: ... The fluorescence signals demonstrating the antibody-receptor complex on the cell surface were quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA).
-
bioRxiv - Cancer Biology 2024Quote: ... cells were stained with 5 μg/ml PI (ST1569-10mg, Beyotime) and immediately analyzed using the CytoFLEX flow cytometer (Beckman Coulter, USA). Tumor cells were distinguished based on GFP positivity or CD45 negativity ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... we used an AV-FITC/PI apoptosis detection kit to detect programmed cell death using flow cytometry (Cytoflex, Beckman Coulter, CA, USA), as previously described (49 ...
-
bioRxiv - Developmental Biology 2022Quote: ... The cell pellets were rinsed twice with PBS and resuspended in PBS containing 0.1% BSA for fluorescence-activated cell sorting (FACS) (MoFlo Astrios EQs, Beckman Coulter, CA, USA). Before LC isolation ...
-
bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
-
bioRxiv - Biochemistry 2023Quote: ... Harvesting of P2 viral stocks was carried out by centrifuging cell suspensions in 50 mL Falcon tubes at 2281 RCF (Beckman GS-15). To the supernatant containing virus ...
-
bioRxiv - Immunology 2023Quote: ... cells were activated with plate-bound anti-CD3 (500 ng/one well of 24-well culture plate; Beckman Coulter, REF# IM-1304) and soluble anti-CD28 (500 ng/ml ...
-
bioRxiv - Cancer Biology 2023Quote: ... Fluorescence of cells was measured using the Gallios flow cytometer system and analyzed using the Kaluza software (Beckman, Lane Cove NSW, Australia).
-
bioRxiv - Cancer Biology 2023Quote: ... Conditioned media were centrifuged at 1000 rpm for 5 min to remove cell debris and then either added to Ultra-Clear Quick-Seal ultracentrifuge tubes (Beckman Coulter 246.344322), 100 kDa molecular weight cut-off centrifugal filters (MWCO ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were incubated further overnight at 20°C and subsequently harvested by centrifugation (Beckman rotor 8.1000, 5000 rpm, 15 min, 4°C). The pellet was resuspended in PBS ...
-
bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
-
bioRxiv - Immunology 2024Quote: Stained cells were analyzed by a CytoFLEX Flow Cytometer with data being captured and processed using the CytExpert Software (Beckman Coulter, Inc). Subsequent analysis of the collected data was performed using FACS Express 6 software (De Novo Software).
-
bioRxiv - Molecular Biology 2024Quote: ... The inhibitors were transferred to the designated wells in 384-well plates with 80% confluent cells using a ECHO525 Acoustic Liquid Handler (Beckman # 001-10080) to a final concentration of 1μM ...
-
bioRxiv - Plant Biology 2024Quote: ... The cell lysate was centrifuged at about 16000 g for 10 min at 5 °C (Avanti J-265 XP, Beckman Coulter, USA). The starch pellet was recovered ...
-
bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
-
bioRxiv - Physiology 2019Quote: ... SVF pools were resuspended in PBS containing 0.5% BSA and 2mM EDTA and 500 000 cells were sampled and stained with antibodies against CD45-ECD (Beckman Coulter, Brea, CA, USA), F4/80-FITC ...
-
bioRxiv - Cancer Biology 2020Quote: Cells were cultured in EV depleted medium (obtained by overnight ultracentrifugation at 100,000g, using a Beckman, XL-70 centrifuge with a 70Ti rotor) for 24h before supernatant collection ...
-
bioRxiv - Genetics 2019Quote: ... as well the cell viability were estimated at 70% of the total CO2 expected using a flow cytometer (Quanta SC MPL, Beckman Coulter, Fullerton, California), equipped with a 488nm laser (22mW ...
-
bioRxiv - Molecular Biology 2019Quote: ... Clarified extracts were prepared by incubating cells with lysis buffer for 15 min on ice followed by centrifugation for 15 min at 16,162 rcf (Beckman Coulter, Microfuge® 16). Extract concentration was quantified by bicinchoninic acid assay (Pierce #23225 ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... The percentage of cells within a defined range of fluorescence intensity was determined with Beckman Coulter CyAn ADP flow cytometer (Beckman Coulter, United States).
-
bioRxiv - Genomics 2021Quote: ... The nuclear sorting was performed at the SciLifeLab Microbial Single Cell Genomics Facility with a MoFlo™ Astrios EQ sorter (Beckman Coulter, USA), as in Montoliu-Nerin et al. ...
-
bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 TI rotor (Beckman ...
-
bioRxiv - Biochemistry 2020Quote: ... was then added to the product which passed microfluidizer and unbroken cells and large debris were eliminated by centrifugation at 4000 × g (Beckman Coulter™, USA). The supernatant containing the IMVs was collected and washed several times with resuspension buffer by centrifugation at approximately 310,000 × g at 4°C for 30 minutes (SORVALL® Centrifuges ...
-
bioRxiv - Cell Biology 2021Quote: ... Tert+ acinar cells were sorted into Tert+ and Tertcells among live cells based on the gate and collected by FACS (MoFlo®Astrios™, Beckman Coulter). C57BL/6J mice were used as negative controls for gating ...
-
bioRxiv - Neuroscience 2019Quote: ... the cell lysates are loaded to the gradients of iodixanol (15%, 25%, 40% and 60%) in the OptiSeal tubes (Beckman Coulter, Indianapolis, IN). Then the lysates were spin down at 67,000 rpm for 1 hour at 18 °C with the ultracentrifuge (Beckman) ...
-
bioRxiv - Bioengineering 2020Quote: ... were generated using high-speed fluorescence-based cell sorting using various staining and gating strategies as described in the text (MoFlo Astrios, Beckman Coulter, Mississauga, ON).
-
bioRxiv - Cell Biology 2021Quote: ... Supernatant was discarded and cells were resuspended in 100 μL FACs buffer and sorted with a CyAn™ ADP Analyser (Beckman Coulter, Inc.) at the UCL GOSICH Flow Cytometry Core Facility ...
-
bioRxiv - Biophysics 2022Quote: ... ILC2s were sorted as PI-Lin-CD45+CD127+CRTH2+CD161+ cells via flow cytometry using MoFlo™ XDP (Beckman Coulter, Brea, CA, USA).
-
bioRxiv - Cancer Biology 2022Quote: ... cells were incubated with an additional 400 µl 1× Binding Buffer and analyzed within 20 min by CytoFLEX S (Beckman Coulter Life Science). At least 1 × 104 cells were analyzed to determine the percentage of apoptotic cells ...
-
bioRxiv - Immunology 2021Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were spun down at 4°C for 15 minutes at 4000 RPM (3040 RCF) in a J6 centrifuge (Beckman Coulter Life Sciences). Supernatant was discarded and pellets were stored in 15ml conical tubes in -80°C ...
-
bioRxiv - Biochemistry 2020Quote: ... Unbroken cells were pelleted by centrifugation at 18,000 RCF for 30 min at 4°C (Beckman centrifuge J-20-XP, Rotor JA16.250, DE). The supernatant was transferred to 45 Tl rotor (Beckman ...
-
bioRxiv - Cancer Biology 2020Quote: ... DNA was extracted from fresh frozen tissue or cell lines using the Agencourt DNAdvance Beckman Coulter kit (#A48705, Beckman Coulter, Brea, CA, USA), fragmented and submitted to a series of enzymatic steps including End-repair and Adenylation ...
-
bioRxiv - Neuroscience 2020Quote: ... 6ml of the cell suspension was carefully pipetted onto the top of the density gradient and centrifuged at 800g (Beckman, Cs-16R Centrifuge) for 30min at 22°C (with acceleration and deceleration rates set at 0) ...
-
bioRxiv - Cell Biology 2021Quote: ... The fluorescence generated by the reduction of resazurin to resorufin by live cells was measured using a Beckman coulter DTX 880 multimode plate reader (Beckman Coulter, Brea, California) (570/600 nm).
-
bioRxiv - Microbiology 2022Quote: ... Infectivity and neutralization titers were determined using ACE2-expressing HEK293 target cells (Integral Molecular, Philadelphia, PA) in a semi-automated assay format using robotic liquid handling (Biomek NXp Beckman Coulter, Brea, CA). Virions pseudotyped with the vesicular stomatitis virus (VSV ...
-
bioRxiv - Microbiology 2022Quote: ... induced by doxycycline (DOX) at 3 μg/ml and sorted for high GFP-expressing cells by MoFLO Astrios EQs (Beckman Coulter Life Sciences) using 488 nm lasers ...
-
bioRxiv - Microbiology 2024Quote: ... Purified PCR products were measured and pooled for sequencing on an Illumina NextSeq 550 High Output Flow Cell using the single-end 75 base technique using AMPure XP SPRI beads (Beckman Coulter Life Sciences).
-
bioRxiv - Microbiology 2024Quote: Cell growth was determined by measuring the absorbance of the culture at A600 (OD600) using a spectrophotometer (DU730, Beckman Coulter, Brea, CA, USA). The concentrations of the products (acetate and ethanol ...
-
bioRxiv - Neuroscience 2024Quote: Neuron cells obtained from brain organoids were screened for cell surface molecule expression using an ultra-high-speed flow cytometry sorter (Beckman, MoFlo Astrios EQS) as previously reported(Menon et al. ...
-
bioRxiv - Immunology 2023Quote: ... The fluorescence signals for the antibody-receptor complex on the cell surface were then quantified by flow cytometry (CytoFLEX, Beckman Coulter, Brea, CA). Data were analyzed using FlowJo™ v10.8 Software (BD Life Sciences) ...
-
bioRxiv - Microbiology 2023Quote: ... The cells were then incubated for 30 minutes with the following antibodies: anti-HIV-1 core antigen (p24)-RD1 (Beckman Coulter catalog # 6604667), anti-TNFα PE/Dazzle 594 (BioLegend catalog # 502946) ...