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Citations for Beckman :
1551 - 1570 of 1570 citations for Peripheral Blood Mononuclear Cell since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... For patients enrolled in the study, the haemoglobin and red cell indices (MCV, MCH were determined and MCHC) using an automated analyser (Beckman Coulter, Durban, South Africa). CD4-cell counts were assessed using BD FACS Count (BD Biosciences ...
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bioRxiv - Evolutionary Biology 2019Quote: ... We performed a two-step nuclear DNA Content Analysis using Partec Buffer (de Laat et al., 1987) and DAPI fluorochrome through a Cell Lab Quanta SC flow cytometer (Beckman Coulter, Fullerton, CA, USA) equipped with a mercury lamp following the protocol of Doležel et al ...
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was isolated from all TP53-KO minor cells treated in F10 matrigel assays using the Agencourt DNAdvance Genomic DNA Isolation Kit (Beckman Coulter, Cat. No. A48705), and the TP53 locus was amplified using the following primers ...
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bioRxiv - Biochemistry 2020Quote: ... Cell lysates were collected and RNA was isolated using the Biomek FxP and the RNAdvance Tissue Isolation kit (Beckman Coulter, USA, p/n A32646). The resulting RNA was quantified using the Nandrop 8000 (Nanodrop ...
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bioRxiv - Microbiology 2021Quote: ... 50 μL of each cell sample was mixed with 1 μL of KC57-FITC antibody that identifies the HIV-1 Gag and p24 (Beckman Coulter Life Sciences, USA) and incubated at 4°C for 20 min ...
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bioRxiv - Immunology 2021Quote: ... isolated CD4+ cells were activated with a combination of plate-bound anti-CD3 (750 ng/24-well culture plate well; Immunotech/Beckman Coulter REF # IM-1304) and soluble anti-CD28 ((1ug/mL ...
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bioRxiv - Systems Biology 2022Quote: ... supernatant containing metabolites extracted from 1.1×109 cells and 11 spike-ins were aliquoted into 1,000 vials using an automated liquid handler (Biomek 4000, Beckman Coulter, Inc., Brea, California, USA), with 5 μL solution per tube ...
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bioRxiv - Biophysics 2023Quote: ... of 0.6 at 260 nm were loaded into cells fitted with sapphire windows and 12 mm double channel epon charcoal centerpieces (Beckman Coulter, Indianapolis, IN, USA). Data were collected in intensity mode at 260 nm ...
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bioRxiv - Biochemistry 2023Quote: ... cells were treated with the drugs (20 µM to a suspension at 500,000 cells/mL in PBS containing 2 % FBS at 37°C) and directly analyzed by flow cytometry (Gallios, Beckman-Coulter, 405/488 nm laser) over 2 h ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1) ...
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bioRxiv - Immunology 2023Quote: ... Suspended cells were incubated in the dark for 30 min at room temperature with CD66b-APC-Alexa Fluor 750 (Beckman Coulter Life Sciences, B08756) for the detection of neutrophils ...
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bioRxiv - Cell Biology 2023Quote: ... where OD550 = 1 corresponds to ∼ 1 × 106 cells ml -1 (David L. Spector, 1998) or a Z1 Coulter Counter (Beckman Coulter Inc., Indianapolis, Indiana). Cultures were generally used during the log phase of cell growth (cell density 2-4 × 105 cells ml-1).
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bioRxiv - Genomics 2023Quote: ... Cell pellets were processed for genomic DNA extraction following the magnetic bead-based Genfind V3 DNA isolation protocol (Beckman Coulter Life Sciences Cat# C34881).Next-generation sequencing was performed on the Illumina MiSeq platform ...
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bioRxiv - Biophysics 2023Quote: ... Cells were harvested by centrifugation at 8000 g at 4°C for 20 min (Avanti® J-E centrifuge from Beckman Coulter, rotor: JLA 16.250).
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bioRxiv - Molecular Biology 2023Quote: ... supernatants from the SF samples and cellfree human and equine neutrophil cell culture supernatants were transferred to SW40 tubes (Beckman Coulter Inc., Brea, United States). They were subjected to centrifugation at 10,000 ×g for 35 min at 4 °C ...
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bioRxiv - Neuroscience 2020Quote: ... for further depletion of cell debris and spun at 10,000 × g for 30 min at 4°C (AH-650 rotor, Beckman Ultra-Clear Tube with 5 ml capacity). The pellet was resuspended in 100 μl PBS and considered to be the “10K pellet” (10K or 10K fraction) ...
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bioRxiv - Biochemistry 2019Quote: ... Resuspended cells were lysed by French Press (8,000 psi, 4°C, 2 passages) and lysates were centrifuged (Beckman 60Ti rotor, 30 min, 50,000 rpm, 4°C). Pellets were resuspended in 100 ml of buffer B (PBS [140 mM NaCl ...
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bioRxiv - Microbiology 2023Quote: ... another 2 mL of staining bacterial samples was analyzed with a flow cytometry to check cell viability (Beckman CytoFLEX FCM, USA, Channel FL2-A: PE-A).
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bioRxiv - Biochemistry 2020Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 37 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 10 °C for 10 min.
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bioRxiv - Biochemistry 2019Quote: ... Protein expression was induced with 0.4 mM Isopropyl β-D-1-thiogalactopyranoside and cells were grown at 25 °C overnight before harvesting by centrifugation at 4000 rpm (Beckman Coulter Avanti JXN-26; JLA8.1 rotor) at 4 °C for 15 min.