Labshake search
Citations for Beckman :
1551 - 1600 of 1639 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were then filtered through 40 μm Falcon™ Cell Strainers (Thermo Fischer Scientific, cat no. 08-771-1) and analyzed by flow cytometry (MACSQuant VYB, Miltenyi or CytoFLEX, Beckman Coulter). Matlab-based software for processing flow cytometry data was developed in-house by Yaron Antebi.
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bioRxiv - Cancer Biology 2021Quote: ... the 197 library compounds dissolved in DMSO or MilliQ (Supplementary Table 1) were added in duplicate using the Echo 550 dispenser (Beckman Coulter). Final drug concentrations ranged standard between 0.1 nM and 10 μM ...
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bioRxiv - Biochemistry 2021Quote: ... live cells were stained with Alexa 647-conjugated M1 anti-FLAG antibody (1:1000, 5 minutes) and analyzed on a Cytoflex flow cytometer (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2020Quote: ... the 4 tubes were centrifuged using JA-20 rotor at 43,000 × g for 1 hour and 30 minutes in Beckman coulter® Avanti j-26 xp (Beckman, France). The pellets of the 4 tubes were preserved at −80°C before transport and metagenome sequencing and analysis.
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bioRxiv - Immunology 2021Quote: ... The PBMC were resuspended in 1 mL cold PBS+BSA per 15 mL whole blood processed and counted with a ViCell (Beckman Coulter) using VersaLyse reagent (Beckman Coulter #A09777 ...
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bioRxiv - Immunology 2021Quote: ... 0.1 ng cDNA was used per sample and the libraries were cleaned up with 1.8x AMPure® XP beads (Beckman Coulter). Quality and integrity of nucleic acids were assessed using the Agilent Technologies 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Immunology 2021Quote: ... either 150 μl or a maximum of 1 × 106 viable lymphocytes were analyzed using a CytoFLEX LX Flow Cytometer (Beckman Coulter), and data was analyzed with FlowJo software (FlowJo LCC ...
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bioRxiv - Synthetic Biology 2023Quote: ... Droplets were made by either manually pipetting 0.05-0.1 μL droplets or dispensing individual 2.5 nL - 20 nL droplets using an Echo 550 acoustic liquid handler (Beckman Coulter, USA) into 2 μL of the lipid-oil phase in 384-well microplate ...
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bioRxiv - Microbiology 2023Quote: ... 1 μl or 10 μL of dilution were added to the 384-well plates using automated dispenser BioMek FX (Beckman Coulter). Extracellular bacteria were then eliminated by adding antibiotic to the media 1h after infection ...
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bioRxiv - Cancer Biology 2024Quote: ... 200 μl of the PCR reaction (roughly corresponding to 1 μg of amplicon) was then purified using a double-sided selection with AMpure XP beads (Beckman Coulter). First ...
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bioRxiv - Microbiology 2024Quote: Intracellular HIV-1 p24 staining was performed on fixed U1 and ACH-2 cells with KC57-FITC (Beckman Coulter, Cat#6604665). After allowing at least 30 minutes for fixation ...
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bioRxiv - Neuroscience 2023Quote: ... The absorbance was recorded at 1 min intervals for 10 min at 340 nm at 30°C using a spectrophotometer (DU 800, Beckman Coulter). The enzymatic activity was determined as the change in absorbance/min/mg protein.
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Biochemistry 2023Quote: GFP fluorescence level (FITC channel, signal amplification = 1) and cell size (FSC channel, signal amplification = 200) were measured with flow cytometer CytoFlex S (Beckman Counter) and CytExpert Software in 10000 cells (per each sample ...
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bioRxiv - Genomics 2023Quote: PCR reaction products were cleaned and size selected using a 0.7:1 ratio of AMPure XP SPRI beads (Beckman Coulter Inc.) to sample ...
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bioRxiv - Plant Biology 2024Quote: ... Then the reaction was started by adding 150 µL of a 1 M proline solution and the decrease in the OD600 monitored until at least 1 min of linear reaction was observed (DU-800 Spectrophotometer, Beckman Coulter). Reaction rates were calculated using the 600 nm absorbance coefficient of DCPIP (19.1 mM-1 cm-1 ...
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bioRxiv - Biochemistry 2022Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... and permeabilized in PBS/1% BSA/0.1% saponin before staining with a FITC-conjugated anti-Cap24 (KC57-FITC, Beckman Coulter, France) for 1h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... to remove intact cells and the supernatant was centrifuged again at 25,000 RCF for 1 hour (Beckman Coulter JA-20 rotor). The pellet was resuspended in lysis buffer and sodium cholate was added to a final concentration of 1% and stirred at 4°C for 1 hour ...
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bioRxiv - Microbiology 2024Quote: ... Four working concentrations were achieved through 1:2 serial dilutions using a Biomek i7 liquid handler (Beckman Coulter, ED. Fig. 1a). Precultures were grown overnight and diluted to an OD600 nm of 1 (WT ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed with PBS at the indicated time and then stained for GPA/Band3/CD49d expression with 1:20 human BV421-anti-235a (Beckman Coulter), 1:20 human FITC-BRIC6 (IBGRL Research ...
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bioRxiv - Molecular Biology 2024Quote: ... OD260 absorbance equal to 60 µg RNA in 200 µl polysome lysis buffer was loaded on 800 µl of 1M sucrose cushion in 1 ml thick wall polycarbonate tubes (part numbers 343778; Beckman Coulter). After centrifugation at 90,000 RPM (352,000 x g ...
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bioRxiv - Biophysics 2024Quote: ... cycled at 2 secs on / 2 secs off) and clarified by centrifugation at 8500 RPM for 1 hour using a fixed angle JA-20 rotor (Beckman Coulter) at 4 °C ...
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bioRxiv - Plant Biology 2024Quote: ... The pre-concentrated culture was centrifuged (10000 g, 10 min) in 1-L buckets (Avanti J-265 XP, Beckman Coulter, USA). The biomass reached a concentration of approximately 40 % (dry weight - DW ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatants were collected and subjected to high-speed centrifugation at 100,000× g for 1 h (TLS-55 rotor, OptimaTMMAX Ultracentrifuge; Beckman Coulter, USA). The supernatants (S100 ...
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bioRxiv - Biochemistry 2024Quote: ... Digested pellets were loaded onto a 10-50% sucrose gradient (sucrose dissolved in 1× lysis buffer) and ultracentrifuged using a SW41 Ti rotor (Beckman Coulter) at 40,000 rpm (273,620 × g ...
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bioRxiv - Cancer Biology 2024Quote: ... Each 384 well plate was then pooled into a 1 μL reaction product and cDNA was purified using 0.8X volume of AMPure XP beads (Beckman Coulter, A63882). Each pool was analyzed for concentration and size distribution using Bioanalyzer (Agilent).
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bioRxiv - Bioengineering 2024Quote: ... Cells were resuspended in 250 uL PBS/1% BSA after the final wash and subjected to analysis by a CytoFLEX flow cytometer (Beckman Coulter).
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bioRxiv - Bioengineering 2024Quote: ... Cells were resuspended in 250 uL PBS/1% BSA after the final wash and subjected to analysis by a CytoFLEX flow cytometer (Beckman Coulter).
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bioRxiv - Bioengineering 2024Quote: ... we transferred the inserts to new wells containing 1 mL of TrypLE to single-cell disperse the clusters and perform cell counts (Vi-Cell XR, Beckman Coulter). We again saved these supernatants ...
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bioRxiv - Cell Biology 2024Quote: ... the inserts were transferred to new wells containing 1 mL of TrypLE to single-cell disperse the clusters and perform cell counts (Vi-Cell XR, Beckman Coulter). The supernatants were again saved ...
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Inhibition of Ribosome Biogenesis in vivo Causes p53-Dependent Death and p53-Independent DysfunctionbioRxiv - Cell Biology 2024Quote: ... 1 mL of the lysates was loaded onto a 10%-50% sucrose gradient in a 14 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a Beckman Optima LE-80K Ultracentrifuge Floor Centrifuge at 261,000 × g for 135 minutes at 4 °C ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter, TLA-120.2 Beckman rotor, 288000 g). The excess monomeric protein and some small oligomers were then removed by multiple filtration steps using 100-kDa cut-off membranes ...
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bioRxiv - Genetics 2021Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean SPRI beads (Beckman Coulter, A63987).
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bioRxiv - Molecular Biology 2021Quote: ... Resulting libraries were pooled and treated with AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume to purify DNA (Beckman Coulter, Brea, CA, USA). Library yield and quality was determined by TapeStation electrophoresis with HSD1000 ScreenTapes (Agilent Technologies).
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bioRxiv - Biophysics 2020Quote: ... The lysate was then centrifuged at 4° C in the SW32Ti rotor with a speed of 30,000 rpm for 1 h to pellet the membrane fraction using an Optima L-90K Ultracentrifuge (Beckman Instruments, Brea, CA). The pellet was resuspended in HND buffer and bulk membrane proteins were extracted by adding 2% ANAPOE-X-100 (Anatrace ...
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Hydrogen sulfide blocks HIV rebound by maintaining mitochondrial bioenergetics and redox homeostasisbioRxiv - Microbiology 2021Quote: ... Permeabilized cells were then incubated with 50 μl of 1:100 dilution of phycoerythrin (PE)-conjugated mouse anti-p24 monoclonal antibody (KC57-RD1; Beckman Coulter, Inc.) for 30 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... anti-CD105 and anti-HLA-ABC antibodies (suppl. Table 1) and analysed by flow cytometry (Cytoflex; Software Cytexpert 2.3, Beckman-Coulter, Krefeld, Germany). Upon passage 3 ...
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bioRxiv - Plant Biology 2020Quote: ... Soil pH and EC were 8.3 and 12 dS m−1 that were measured by a pH meter (Beckman 45 Modal, US) and EC meter (VWR Conductivity Meter DIG2052 ...
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bioRxiv - Microbiology 2020Quote: ... Membrane-containing fractions were collected in 1 mL after ultracentrifugation at 35,000 rpm on an SW-40 rotor (Beckman, Brea, CA USA) for 6 hours at 4°C and stored at -80°C prior to analysis.
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bioRxiv - Evolutionary Biology 2021Quote: ... with NEBNext Multiplex Oligos for Illumina® (Dual Index Primers Set 1) and AMPure XP magnetic beads (Beckman Coulter, Brea, CA, USA). DNA content and quality of indexed libraries were examined with a Qubit 4.0 using the high-sensitivity kit (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and libraries were purified and size selected using Ampure XP Beads at a 1.8:1 bead volume:library volume (Beckman Coulter, Brea, CA, USA). Library quality and transposition efficiency was accessed using a Fragment Analyzer and PROSize 2.0 (Agilent ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 2000 rpm (931 x g) [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A] for 2 hours at 30°C ...
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bioRxiv - Microbiology 2022Quote: ... Cells were permeabilized in 0.5% Triton-X for 10 min and stained with 1:300 KC57-FITC (Beckman Coulter 6604665; RRID: AB_1575987) or 1:300 KC57-RD1 (numbers) ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...