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Citations for Beckman :
1451 - 1500 of 1639 citations for 1 4 Nitrophenyl cyclobutanecarbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... the cross-linked material was loaded onto glycerol-tartrate gradients as described elsewhere 53 and centrifuged for 1 h at 25,000 rpm in an SW-40 rotor (Beckman), with brakes set at the slowest possible deceleration ...
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bioRxiv - Immunology 2023Quote: ... SSM liquid culture was incubated for 24 hours at 36°C with constant shaking at 180 rpm until reaching mid-log phase OD600nm 0.5 with 1 cm path width (Beckman Coulter™ DU 530 UV Vis spectrophotometer) ...
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bioRxiv - Developmental Biology 2024Quote: ... in 1×MMR final were manually prepared in pre-chilled 14 mL ultra-clear centrifuge tubes (Beckman Coulter, #344060) in the following order from the bottom to the top of the tube ...
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bioRxiv - Genomics 2024Quote: ... They were transduced via spinfection at 1000 rpm for 1 hour at 30 °C (Beckman Coulter, Allegra X-14R), followed by overnight incubation ...
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bioRxiv - Synthetic Biology 2024Quote: ... The suspension was incubated at room temperature rotating for 1 h and then ultracentrifuged (38,000 rpm; Beckman 70Ti rotor) for 1 h at 4 °C ...
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bioRxiv - Microbiology 2024Quote: ... the solution was neutralised by the dropwise addition of 1 M hydrochloric acid centrifuged at 34,000 g (JA-20 rotor, Beckman) at 20 °C for 30 minutes to yield P5 and S5 ...
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bioRxiv - Microbiology 2024Quote: ... Intracellular p24Gag was stained using FITC-conjugated mouse anti-p24Gag monoclonal antibody (KC57; Beckman Coulter, catalog # 6604665, 1:25) as previously described [88] ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed and resuspended in PBS 1% FBS 2mM EDTA and run on a Cytoflex LX (Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... For p24 staining (RD1-conjugated mouse anti-p24 clone KC57, cat# 6604667, 1:100 dilution, Beckman Coulter, Brea, CA), cells were fixed and permeabilized using the Cytofix/Cytoperm kit (BD Biosciences ...
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bioRxiv - Microbiology 2024Quote: ... Amplicons were visualized on a 1% agarose gel and purified with Agencourt AMPure XP beads (Beckman Coulter, Brea, CA). The concentration of purified amplicons was quantified using the Qubit High Sensitivity dsDNA kit and a Qubit Fluorometer (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... the CP was centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TLA-100.3 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... the samples were centrifuged at 72,000 rpm (280,000 × g) for 1 h at 5 °C in a TL-100.1 rotor (Beckman Coulter, USA) on a TL-100 ultracentrifuge to pellet the reassembled VLPs ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were washed once more with 1% BSA in PBS and analyzed using a Gallios flow cytometer (Beckman Coulter) to determine EdU incorporation and DNA content ...
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bioRxiv - Cell Biology 2024Quote: ... The DNA product was then purified with a 0.8:1 volumetric ratio of AMPure XP Beads (Beckman Coulter, A63881) and the whole volume was resuspended in 20ul of PCR mastermix (KAPA HiFi Readymix ...
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bioRxiv - Biophysics 2020Quote: ... Very small amounts of fibrillar species formed during this time were removed by ultracentrifugation for 1 h at 90000 rpm (Optima TLX Ultracentrifuge, Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The amount of p24 capsid protein was determined by the HIV-1 p24 ELISA antigen assay (Beckman Coulter, Fullerton, CA). Virus from different productions averaged 175 ng/µl of p24 antigen.
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bioRxiv - Bioengineering 2021Quote: ... Cultures were grown in a MTP-48-BOH 1 FlowerPlate in a BioLector Pro (both Beckman Coulter Life Sciences, USA) at 1400 rpm ...
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bioRxiv - Biochemistry 2020Quote: ... After increasing the NH4Cl concentration of the supernatant to 1 M to dissociate bS1 from 70S ribosomes the sample was centrifuged in a Type 70 Ti rotor (Beckman) at 60,000 g for 2 hours ...
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bioRxiv - Systems Biology 2020Quote: ... GFP productivity was determined by measuring the GFP signal (FL-1 channel) of cells by flow cytometry (Gallios, Beckman Coulter).
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bioRxiv - Microbiology 2021Quote: ... and stained intracellularly using PE-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) in combination with Nef or Vpu rabbit antisera (1:1000 dilution) ...
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bioRxiv - Genomics 2021Quote: ... 1 mM EDTA (corresponding to a refractive index of 1.3700) and loaded into OptiSeal polypropylene centrifuge tubes (Beckman Coulter, 362185). Centrifugation was performed in a near-vertical NVT-90 rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2022Quote: SpeMreBs with a volume of 200 μL were polymerised for 1 to 6 h and centrifuged (100,000 rpm, 23°C, 120 min) in a TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 200 μL of water ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Biochemistry 2022Quote: ... The lysed cells were centrifuged at 41399 g and 6 °C and for 1 hour (Beckman Avanti J-25 centrifuge) to obtain the cytoplasmic protein extract.
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bioRxiv - Cell Biology 2022Quote: ... were mixed 1:1 with liposomes (6 mg/ml) and floated for 2h at 55 000 rpm in a TLS-55 rotor (Beckman) at 25°C through a sucrose gradient ...
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bioRxiv - Microbiology 2021Quote: ... culture samples were diluted (1:100 or 1:1000) with 0.2-µm-filtered 18% BSW and were analysed with a Multisizer M4 Coulter cytometer (Beckman-Coulter) equipped with a 20 or 30 µm aperture tube ...
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bioRxiv - Cell Biology 2020Quote: ... After purification of the PCR product with a 1: 0.6 ratio of PCR product to AMPure XP beads (Beckman Coulter), the success of cDNA preparation was confirmed using a 2100 Bioanalyzer with High-sensitivity DNA chip (Agilent) ...
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bioRxiv - Neuroscience 2020Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g 1 h).
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bioRxiv - Neuroscience 2022Quote: ... The remaining supernatant (conditioned medium) was subjected to ultracentrifugation (UC) (100,000 x g, 1 h) using rotors Ti45 or SW32Ti (Beckman Coulter). The pellet was rinsed with PBS and spun again using rotor SW55Ti (100,000 x g ...
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bioRxiv - Biophysics 2021Quote: ... Cells were lysed and the lysate was clarified by centrifugation for 1 h at 100,000g (45 Ti rotor, Beckman Coulter). The clarified lysate was incubated with Ni2+-NTA agarose resin for 1 h at 4 °C ...
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bioRxiv - Synthetic Biology 2021Quote: ... Libraries corresponding to samples to be directly compared to each other (e.g. DMSO vs UNC7040) were pooled together and purified using 1 volume of Agencourt AMPure XP (Beckman Coulter). The pooled libraries were eluted with 23 µL of elution buffer (NEXTflex ChIP-Seq kit ...
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bioRxiv - Microbiology 2020Quote: ... The cell lysate was collected and centrifuged at 100,000 x g for 1 h in an ultracentrifuge (Model XL-90, Beckman Coulter). The supernatant was collected ...
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Heatrich-BS enables efficient CpG enrichment and highly scalable cell-free DNA methylation profilingbioRxiv - Genomics 2021Quote: ... the sample was incubated at 25°C for 1 hour and then cleaned up by performing two rounds of 1.2x SPRI Select (Beckman Coulter). The sample was then subject to bisulfite conversion following the recommended protocol of Zymo EZ DNA Methylation kit (Zymo Research) ...
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bioRxiv - Genomics 2021Quote: ... The cDNA was purified using RNA XP beads purification with 1:1.8x ratio of sample to beads (Beckman Coulter, A63987) and eluted in 24.5 µl water ...
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bioRxiv - Neuroscience 2021Quote: ... and centrifuged at 250,000 g for 1 hour at room temperature with a TLA 120.2 rotor using an Optima XP benchtop ultracentrifuge (Beckman Coulter). Supernatant were collected by pipetting ...
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Genomics 2020Quote: ... This sequencing-ready NGS library was purified using 1 round of a 1X proportion of AMPure XP beads (Beckman Coulter). The final elution volume was 17 µl in Elution Buffer.
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bioRxiv - Microbiology 2021Quote: ... followed by purification with a 2.2:1 volumetric ratio of RNAClean XP solid phase reverse immobilization (SPRI) beads (A63987, Beckman Coulter). Copy numbers of resulting cDNAs were determined by limiting-dilution PCR using fluorescence-assisted clonal amplification (FCA ...
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bioRxiv - Microbiology 2020Quote: ... through a 1 ml 30% sucrose cushion (in phosphate-buffered saline, PBS, pH 7.4) in a SW41 rotor (Beckman coulter). The pelleted virus material was re-solubilized in PBS and analyzed by sodium docecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with following antibody mix for 30 minutes on ice: pan-γδTCR-PE (1:10; IMMU510; Beckman Coultier) and CD69-APC (1:25 ...
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bioRxiv - Cell Biology 2023Quote: A sucrose gradient was prepared by mixing 3 ml of liver homogenate with 1 ml of HLM containing 60% sucrose in a 13.2 ml ultracentrifuge tube (Beckman Coulter), followed by 5 ml and then 4 ml of HLM buffer containing 5% and 0% sucrose ...
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bioRxiv - Microbiology 2023Quote: ... Not solubilised protein was removed by a second pelleting at 100×kg for 1 h in Ti70 rotor (Beckman Coulter). The solubilized and denatured protein was loaded on a 5 ml HisTrap HP column (Cytiva) ...
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bioRxiv - Neuroscience 2022Quote: ... DNase-treated RNA was subsequently purified using RNase-free Agencourt AMPure XP beads (Beckman Coulter; 2:1 volume of beads:sample).
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 1 hr at RT before centrifugation at 100,000 x g (23°C) in a TLA100 rotor (Beckman-Coulter). The top 50% of the supernatant was removed ...
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bioRxiv - Developmental Biology 2023Quote: ... Libraries were purified using a ratio of 1:0.85 sample to bead ratio using Agencourt AMPure XP SPRI beads (Beckman Coulter). The final products were quantified on a D5000 Tapestation (Agilent ...
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bioRxiv - Neuroscience 2023Quote: ... Homogenized tissue was sonicated 20 times with 1 sec pulses and then centrifuged using a SW55 Ti rotor (Beckman Coulter) at 42,700 rpm for 15 min at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was separated into soluble and insoluble fractions by ultracentrifugation at 100,000 x g for 1 h using Optima L-XP (Beckman Coulter). The soluble fraction was filtered using a bottle-top 0.22-µm filter (Corning) ...
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bioRxiv - Molecular Biology 2022Quote: ... and purified according to the previously mentioned bead clean-up procedure (using 0.6 to 1-fold AMPure XP bead suspension, A63880, Beckman Coulter), except that after removal of ethanol ...
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bioRxiv - Molecular Biology 2022Quote: ... Transposition was blocked by the addition of 1% SDS and DNA purified with Agencourt AMPure XP beads (Beckman Coulter, A63881) according to the manufacturer’s instructions using a 2:1 ratio of beads to sample ...
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bioRxiv - Plant Biology 2024Quote: ... using a standard protocol but with all reaction volumes reduced by 1/10 to accommodate the automation of the prep on the Echo LabCyte liquid handler (Beckman). Libraries were purified using Agencourt Ampure XP beads (Beckman Coulter) ...