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Citations for Beckman :
101 - 150 of 186 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Plant Biology 2022Quote: ... Sucrose step gradient (sucrose in resuspension buffer) were prepared in Ultra-Clear™ centrifuge tube 11×89 mm (Beckman Coulter) starting from the bottom (%w/w) ...
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bioRxiv - Molecular Biology 2023Quote: ... using 10-40 % sucrose gradients in buffer A after 3 hours of centrifugation at 35,000 rpm and 4 °C in SW41 rotor (Beckman Coulter). To isolate ribosomes for structural analysis ...
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bioRxiv - Molecular Biology 2022Quote: ... or a 384-well plate containing 1x Takara lysis buffer (STORM-seq) using a MoFlo Astrios cell sorter running Summit v6.3 (Beckman Coulter). The Astrios was equipped with a 100 um nozzle at 25 psi ...
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bioRxiv - Microbiology 2023Quote: ... Frozen lysates were thawed on ice and loaded on 10-50% sucrose gradients in Polysome buffer and subjected to ultracentrifugation at 36,000 rpm in an SW41.Ti swinging bucket rotor (Beckman Coulter) for 150 min at 4°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The ribosomes with equal A260 in each group were loaded onto a sucrose gradient (10–50%) buffer prepared using a gradient station and centrifuged in an SW41Ti rotor (Beckman) at 4°C for 4 hours at 28,000 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Staining was followed by reconstitution in FACS buffer and flow cytometry readings on a Cytoflex V5-B5-R3 (Beckman Coulter) and data was analyzed with FlowJo (version 10.8.2 ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on either a Cytoflex (Beckman Coulter Life Sciences ...
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bioRxiv - Biochemistry 2023Quote: ... made in TEMB buffer) and ultracentrifuged at 105,000 x g for 35 min (SW 32 Ti Swinging-bucket, Beckman Coulter). Gradients were fractionated ...
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bioRxiv - Physiology 2023Quote: ... and HDL (1.063
buffers in the Optima MAX-TL Ultracentrifuge with the TLA-100 rotor (Beckman Coulter). Complete blood count analysis was carried out using Heska Element HT5. -
bioRxiv - Molecular Biology 2023Quote: ... Digested lysates were layered onto 50 ul 1M sucrose cushions prepared in IP buffer and ribosomes were pelleted by centrifugation at 70,000rpm in a TLA 100 rotor (Beckman Coulter) for 30 min at 4°C.
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bioRxiv - Microbiology 2023Quote: ... sucrose gradients prepared with buffer C and centrifuged for 16 h at 71,935 g at 4 °C in a SW28 rotor (Beckman Coulter). Gradients were fractionated on a Biocomp Piston Gradient Fractionator (BioComp Instruments ...
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bioRxiv - Neuroscience 2023Quote: ... resuspended in FACS buffer containing DAPI and analyzed using a Beckman Coulter CytoFLEX flow cytometer (Beckman Coulter, Brea, CA, USA). The specificity of each antibody was validated on activated rat splenocytes and peritoneal macrophages (intraperitoneal thioglycollate 2% ...
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bioRxiv - Immunology 2024Quote: ... and nanocontainers were then further purified by sucrose density ultracentrifugation (10-40% w/v gradients prepared in 50 mM potassium phosphate buffer at pH 7.5) in a SW-32 rotor (Beckman Coulter) spinning at 28,000 rpm for 4 hours at 4 °C ...
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bioRxiv - Neuroscience 2024Quote: ... the pellet was suspended in 250 μl of cold FACS buffer and the resulting solution was finally sorted on a MoFloAstrios device (Beckman) to reach a concentration of 410 cells/μl.
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bioRxiv - Pathology 2024Quote: RNA from rat liver tissue was snap frozen in RNAlater and lysed using lysis buffer (RNAdvanced Kit, A32646, Beckman Coulter), 20µL Proteinase K on a TissueLyser II (Qiagen ...
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bioRxiv - Biochemistry 2020Quote: ... glycerol gradient prepared in lysis buffer (without triton) with respective amounts of glycerol using SG15 Hoefer Gradient Maker and centrifuged using SW50 (Beckman Coulter) rotor at 45,000 rpm for either 13 hours or for 14 hours 48 minutes at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... then 2ml of 40% sucrose (in buffer C) and 1ml of 20% sucrose (in buffer C) were sequentially overlaid and the SW55 tubes (13×51 mm, Beckman Coulter) were centrifuged at ∼150,000×g (38,500 RPM ...
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bioRxiv - Molecular Biology 2019Quote: ... The cleared supernatant was layered over 15-40% sucrose gradients in disassociation buffer and spun 28000 rpm for 16 hours at 4°C in a SW-32 rotor (Beckman-Coulter). Gradients were fractionated with an ISCO fractionation system and 30S + 50S peaks were combined ...
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bioRxiv - Biochemistry 2019Quote: ... The resulting supernatant was loaded onto a 32% sucrose cushion prepared in Buffer A and centrifuged at 28,000 rpm (Beckman SW41Ti rotor) for 16 h ...
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bioRxiv - Genomics 2021Quote: ... After one wash with MACS buffer the percentages of IAV infected cells were determined with a MoFlo Astrios Cell Sorter (Beckman Coulter) and the uninfected gating control was defined using uninfected cells stained with both the primary and the secondary antibodies.
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bioRxiv - Neuroscience 2020Quote: ... Each pool of samples was then purified using 79 μL beads buffer (20% PEG-8000 in 2.5 M NaCL, final concentrations) and 50 μL Ampure XP Beads (Beckman Coulter, A63881), and eluted in 7 μL NF H2O.
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bioRxiv - Biochemistry 2020Quote: ... in BC300N buffer and centrifuged for 4hr at 55,000rpm at 4°C in a TLS-55 rotor (with Beckman 356860 adaptors). 40µl fractions were collected by pipetting from the top ...
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bioRxiv - Biochemistry 2020Quote: ... To that was dispensed TMPRSS2 (150 nL) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give total reaction volume of 5 µL ...
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bioRxiv - Microbiology 2020Quote: ... The 150 μl of sample/lysis buffer was added to a well of a 96-well plate containing 50 μl of magnetic beads (AMPure XP, Beckman Coulter). After thorough mixing by pipetting up and down at least 10 times ...
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bioRxiv - Immunology 2022Quote: ... Samples are washed with 1X FACS buffer again to remove the PFA and finally re-suspended in 1 x FACS buffer for flow cytometry analysis using either Fortessa instrument (Becton Dickinson) or CytoFlex LX instrument (Beckman Coulter) fitted with an automated sampler accommodating 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... The 150 μl of sample/lysis buffer was added to a well of a 96-well plate containing 50 μl of magnetic beads (AMPure XP, Beckman Coulter). After thorough mixing by pipetting up and down at least 10 times ...
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bioRxiv - Biophysics 2021Quote: ... The supernatant was then purified by loading it on a cushion of 25% sucrose (in TNE buffer) and ultracentrifuged at 100000g for 100 minutes (SW41Ti, Beckman Coulter) at 4° C ...
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bioRxiv - Microbiology 2022Quote: ... Pellets were resuspended in 5 ml SM buffer and purified via CsCl density gradient ultracentrifugation (Optima XPN-80 ultracentrifuge; Beckman Coulter) at 19,200xg for 18 h at 10°C ...
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bioRxiv - Immunology 2020Quote: ... cryopreserved PBMC were recovered overnight in RPMI1640 supplemented with 10% FBS and washed in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). The excitation wavelength for the generation of side-scatter (488/6-nm filter ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed twice in cold phosphate buffer (PBS) before suspended in cold PBS and sorted on Moflo Astrios (Beckman Coulter). Sorted cells with different scattering intensity were stained to identify the percentages of CD3+ CD4+ T/CD3+ CD8+ T ...
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bioRxiv - Pathology 2020Quote: ... 200 μl were added to LBF lysis buffer (supplied with the kit) and viral RNA was extracted using RNAdvance Viral Kit (Beckman Coulter) and further processed on the Biomek i7 Automated Workstation (Beckman Coulter) ...
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bioRxiv - Cancer Biology 2020Quote: ... The cells were then washed with and re-suspended in 500 µl flow cytometry buffer and analyzed using a BC CytoFLEX flow cytometer (Beckman Coulter).
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bioRxiv - Synthetic Biology 2021Quote: ... The clarified lysate was layered onto a 1 ml cushion of 30% iodixanol (OptiPrep) in Buffer A in clear ultracentrifuge tubes (3.5 ml thickwall polycarbonate tubes, Beckman Coulter #349622). Ultracentrifugation was run for 3 hours at 100,000 g ...
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bioRxiv - Cancer Biology 2022Quote: ... 50uL Qiagen buffer PB was added to each sample to stop the reaction and DNA was isolated with AMPure XP beads (Beckman Coulter). 15 cycles of PCR were performed with transposed DNA using the dual index primers and NEBNext PCR Master Mix ...
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bioRxiv - Biophysics 2022Quote: ... the 30S and 50S subunits were separated on a 15-35% (w/v) sucrose gradient in dissociation buffer on a SW-32 rotor (Beckman-Coulter) spun at 28,000 rpm (97,000 xg ...
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bioRxiv - Bioengineering 2022Quote: ... followed by a wash step with FACS buffer and analysis at a “Gallios” flow cytometer (Beckman Coulter Life Sciences, United States). The following antibodies were used at a dilution of 1:250 in FACS buffer:
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bioRxiv - Cell Biology 2022Quote: ... and the pellet was resuspended with 5 ml buffer B and transferred into a 14 ml ultracentrifuge tube (331372, Beckman Coulter). 9 ml Buffer C (1.8 M sucrose ...
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bioRxiv - Genomics 2023Quote: ... The parasites were then washed twice with an ice-cold WI buffer (UP03-10, Dojindo EU) and immediately analysed by CytoFLEX flow cytometer (Beckman Coulter). Non-GFP P ...
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bioRxiv - Microbiology 2022Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Microbiology 2022Quote: ... and 25% (wt/wt in 12.5ml) sucrose gradient in 25 mM citrate buffer in 38 ml Ultraclear tubes (Beckman-Coulter, 344058). The gradient was centrifuged at 100,000 × g for 18 h at 4°C (Beckman-Coulter XPN-80 Ultracentrifuge ...
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bioRxiv - Microbiology 2023Quote: Samples were subjected to 750 μl of lysis buffer supplied with the GenFind V2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.). The suspension was vortexed for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... and 400 μL was loaded onto a 5-30% (w/v) buffered sucrose gradient (in Buffer B) in a 14 mm × 89 mm thin-wall Ultra-Clear tube (Beckman # 344059) that was formed using a Biocomp Gradient Master ...
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bioRxiv - Molecular Biology 2023Quote: ... Cells were washed twice with PBS before resuspension in MACS buffer for subsequent flow cytometric analysis using the CytoFLEX (Beckman Coulter).
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bioRxiv - Microbiology 2023Quote: ... the cells were resuspended in 300 μL of FACS buffer and analyzed by using the CytoFlex cytometer (Beckman Coulter, Fremont, CA). Approximately 100,000 events were acquired for each sample ...
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bioRxiv - Microbiology 2023Quote: ... the DNA was diluted to 40 μl with TE buffer and purified with 1.5× vol of AMPure beads (Beckman Coulter, A63882). The eluted DNA was used for sequencing library preparation with NEBNext Ultra II DNA Library Prep Kit (New England BioLabs ...
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bioRxiv - Molecular Biology 2023Quote: ... A 10-30% gradient of glycerol in HEMG buffer was poured into 14 x 89 mm polyallomer centrifuge tubes (Beckman #331372). The resuspended nuclear extract was carefully layered on top of the gradient and centrifuged in an SW41 rotor at 40,000 rpm for 16 hours at 4°C ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 1% FBS in PBS antibody staining buffer and stained with the following antibodies: CD64 PE-Cy7 (Cat. # B06025, Beckman Coulter); CD45 PerCP (Cat ...
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bioRxiv - Genetics 2024Quote: ... Resulting cell nuclei were gated for G1 phase (as determined by Hoechst and propidium iodide staining) and sorted into wells of 96 wells plates containing freezing buffer on a MoFlo Astrios cell sorter (Beckman Coulter). 30 nuclei of each were deposited per well ...