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Citations for Beckman :
51 - 100 of 186 citations for Western Blot Strip It Buffer since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 50 μL of 1X SN buffer on top) in a thin-walled ultracentrifuge tube (Beckman Coulter; 355090), and centrifuging them at 48,000 rpm for 4 hrs at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... Then the ribosomes were pelleted through a sucrose cushion (1.1 M sucrose in HEPES:Polymix buffer with 15 mM Mg2+) at 50,000 rpm for two hours (TLS-55, Beckman), the pellet was dissolved in HEPES:Polymix buffer (5 mM Mg(OAc)2) ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 200μL of FACS buffer and analyzed by flow cytometry using the CytoFLEX flow cytometer (Beckman Coulter).
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bioRxiv - Evolutionary Biology 2020Quote: ... and washed in 5 volumes of chloroplast wash buffer (8 °C, manual stop when reaching 3000 RPM, Beckman JA-20 ...
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bioRxiv - Immunology 2023Quote: Cells were washed 1× with FACS buffer after staining and acquired on a CytoFLEX S cytometer (Beckman Coulter).
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bioRxiv - Cancer Biology 2023Quote: ... resuspended in fresh DPBS buffer containing 1% FBS and analyzed on a CytoFLEX S flow cytometer (Beckman Coulter). Dead cells were excluded by 4′,6-diamidino-2-phenylindole (DAPI).
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bioRxiv - Biochemistry 2023Quote: ... A discontinuous sucrose gradient was formed by overlaying with 6 ml of 35% sucrose and 3.5 ml of 5% sucrose in the same buffer containing 0.25 M Na2CO3 and centrifuged at 38,000 rpm for 18 h in an SW41 rotor (Beckman). Twelve fractions were collected from top to bottom ...
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bioRxiv - Biophysics 2022Quote: ... We purified the folded chassis by glycerol gradient sedimentation by centrifugation[47] through a 10-45% glycerol gradient in TBE buffer supplemented with 11 mM MgCl2 for 130 min at 242,704g in a SW50.1 rotor (Beckman) at 4°C and collected in fractions ...
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bioRxiv - Genomics 2023Quote: ... a buffer exchange is recommended by using 1.8X sample-to-bead ratio of AMPure XP beads (Beckman Coulter) and a prolonged binding time (15 min ...
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bioRxiv - Cell Biology 2020Quote: ... and 25% Histodenz™ in SHE buffer was layered in 14 Å∼ 89 mm Ultra-Clear centrifuge tubes (Beckman) and the tubes were sat at 4°C for 3–4 h to allow the Histodenz™ to diffuse ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... diluted 5-fold in buffer A supplemented with 1 mM phenylmethane sulfonyl fluoride (PMSF) and centrifuged 40,000 rpm (45 Ti rotor, Beckman) for 60 min at 4°C to pellet vesicles ...
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bioRxiv - Biochemistry 2022Quote: ... by dissolving the yeast pellet in 100 mL wash buffer per 100 g cell pellet and centrifugation (JLA 8.1000 rotor, Beckman) at 5,000 rpm ...
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bioRxiv - Cell Biology 2022Quote: ... 200 μL of extract was loaded on the 11 mL 5–20% sucrose gradient in the XB buffer and subjected to ultracentrifugation for 17 h at 197,000 ×g in the swinging bucket rotor SW41 Ti (Beckman). 0.5 mL fractions were collected and concentrated by using trichloroacetic acid precipitation with insulin as a carrier ...
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bioRxiv - Cell Biology 2019Quote: ... Reactions were placed on top of a cushion buffer and centrifuged at 15,600 x g (Optima TL100; Beckman Coulter) for 10 min at 25°C ...
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bioRxiv - Immunology 2020Quote: ... the cells were washed twice with FACS buffer and then labelled with anti-CD3 ECD (Beckman Coulter, Brea, CA), anti-CD4 APC Ax750 (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... resuspended in 250 µl FACS buffer with 1 µl DAPI and analyzed with a Gallios flow cytometer (Beckman Coulter)
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bioRxiv - Microbiology 2022Quote: ... 400 μL of 1X binding buffer was then added before analysing using a CytoFLEX S flow cytometer (Beckman Coutler). Annexin V-FITC positive cells were designated as early apoptotic ...
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bioRxiv - Microbiology 2022Quote: ... Samples were washed and re-suspended in 200 µl wash buffer and analyzed by flow cytometry (Beckman Coulter, USA).
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
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bioRxiv - Microbiology 2023Quote: ... DNA was size-selected by cleaning up with 0.45x volume of Ampure XP buffer (Beckman Coulter, Brea, CA, USA) and eluted in 60 μl EB buffer (Qiagen ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The cells were washed and resuspended in FACS buffer before being analyzed through flow cytometry (Beckman Coulter, Pasadena, USA). For immunofluorescence staining ...
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bioRxiv - Cancer Biology 2021Quote: ... The resulting cell extracts were layered over a 20% sucrose cushion (polysome buffer + 20% w/v sucrose) and ultracentrifuged (Beckman Coulter TLA100.3 Fixed-Angle Rotor ...
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bioRxiv - Cancer Biology 2022Quote: ... made of Sucrose Gradient Buffer (25mM Tris-HCl, pH 7.5, 150mM NaCl, 12mM MgCl2, 1mM DTT) in Ultra-Clear ultracentrifugation tubes (Beckman Coulter). The cytosolic fraction samples were fractionated by ultracentrifugation for 2h 45min at 39,000rpm and at 4°C in a Beckman Optima ultracentrifuge with a SW41 Ti swinging bucket rotor at the following settings ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 22x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 50 μL ...
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bioRxiv - Biochemistry 2022Quote: ... To that was dispensed TMPRSS2 (reconstituted in 50% glycerol at 8.75 μM, 50x) in assay buffer (50 mM Tris pH 8, 150 mM NaCl, 0.01% Tween20) using a BioRAPTR (Beckman Coulter) to give a total reaction volume of 5 μL ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were resuspended in BD perm/wash buffer for subjecting to flowcytometric analysis using Cell Lab Quanta SC (Beckman Coulter). Cell cycle was determined by DNA contents ...
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bioRxiv - Microbiology 2019Quote: ... Then 0.8 mL of Cold 60% sucrose buffer A was added to RNAse-free 5 mL Ultraclean tubes for ultracentrifugation in a SW55Ti (Beckman). The ribosome-containing supernatant was used to fill these tubes and an ultracentrifugation step was performed for 16 hs at 150.000g ...
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bioRxiv - Biochemistry 2019Quote: ... the cells were dissolved in the same buffer as for TtBac-WT 1-123 and spun for 30 min at 9k rpm in a Ti45 rotor (Beckman). The pellet was dissolved in the same buffer and from here onwards the same protocol as for TtBac-WT 1-123 was used.
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bioRxiv - Biochemistry 2019Quote: ... Reactions were spun over 15-30% sucrose gradients made in buffer C with appropriate MgCl2 concentrations at 178,000 g (SW-41 rotor, Beckman Coulter) for 3 hours ...
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bioRxiv - Biophysics 2021Quote: ... solubilized supernatant was collected and subjected to sucrose gradient ultracentrifugation (10% to 50% (w/v) sucrose in buffer A with 0.03% (w/v) digitonin) at 35000 rpm (sw41 Ti rotor, Beckman Coulter) for 16 hours at 4℃ ...
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bioRxiv - Microbiology 2021Quote: ... The supernatant was transferred to an ultracentrifuge tube and overlayed with layers of Lysis Buffer A with 12% Ficoll and Buffer B with 8% Ficoll (AAB2209518, Fisher) and centrifuged at 28,500 rpm for 45 min (Beckman 42.1). Afterwards ...
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bioRxiv - Microbiology 2021Quote: ... and 1.3 mL of 5% (v/v) glycerol in TNE buffer) in 5 mL thin-walled Ultraclear centrifuge tubes (Beckman Coulter). Viral nucleocapsids were pelleted by centrifugation at 23,000 rpm in an SW 55 Ti rotor for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: 5-20% sucrose gradients were prepared on a Biocomp Gradient Station IP from 5% and 20% solutions of sucrose in gradient buffer (50mM Tris HCl pH7.5, 150mM NaCl, 1mM EDTA) to Seton tubes (Beckman Coulter). Single cell suspensions of approximately 3×108 cells from thymus and bone marrow were obtained as above ...
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bioRxiv - Biophysics 2022Quote: ... the homogenized solution was brought to 55 mL with more Percoll buffer and centrifuged at 48400 g for 30 minutes in a 45 Ti rotor (Beckman). Chromosomes appeared as a faint band about 1/5th from the bottom of the centrifuge tube ...
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bioRxiv - Neuroscience 2022Quote: ... Lysates were loaded on 10-50% sucrose gradients in Polysome buffer and subjected to ultracentrifugation at 36,000 rpm in an SW41.Ti swinging bucket rotor (Beckman Coulter) for 150 min at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Stained cells were resuspended in sort buffer at 1 × 106/ml for fluorescence analysis on a Cytoflex S cell analyser (Beckman). SARS-CoV-2 Spike variants were generated by site-directed mutagenesis with Q5 polymerase (New England Biolabs ...
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bioRxiv - Neuroscience 2022Quote: ... all the remaining TL was loaded onto the top of a 1 mL 40% sucrose pad and additional ∼ 750 µL (adjusted to balance all ultra-centrifuge tubes) of buffer A was layered on the top in ultra-centrifugation tube (Beckman Coulter Ultra-Clear centrifuge tubes ...
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bioRxiv - Biophysics 2022Quote: ... The virion was sedimented twice through a 30% sucrose band to 50% band in PBS buffer by ultracentrifugation (Beckman, IN) at 100,000 g ...
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bioRxiv - Biochemistry 2020Quote: ... the crude mitochondria were undergone a sucrose gradient in SEM buffer for 1h at 139,400 x g with an SW40 Ti rotor (Beckman Coulter). Mitochondrial band was pooled and stored at −80 °C.
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bioRxiv - Cancer Biology 2020Quote: ... samples were suspended in FACS buffer (PBS, 20 mM EDTA pH 8.0, 2% FCS) and acquired using a CyAn ADP9 flow cytometer (Beckman Coulter), FACS LSRII Fortessa or FACSymphony (both BD Biosciences) ...
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bioRxiv - Cancer Biology 2020Quote: ... cellular nucleic acids from each lysed single cell were extracted from TCL lysis buffer using a 2.2x (v/v) RNA SPRI (RNA-clean AMPure beads, Beckman-Coulter). After ...
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bioRxiv - Cell Biology 2019Quote: ... the extracted proteins were dialyzed into NaLow and then were loaded onto a 5-20% sucrose gradient made in NaLow buffer and centrifuged at 35,000 rpm for 16 h in a SW41Ti rotor (Beckman Coulter). Gradients were fractionated (0.5 ml fractions ...
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bioRxiv - Molecular Biology 2021Quote: ... Sucrose solutions were made in Buffer G and 25 x 89 mm 38.5 mL open-up polyallomer tubes were used (Beckman Coutler). Ultra-centrifugation was performed (160,000 x g ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were washed twice and resuspended in FC buffer containing 7AAD (LifeTechnologies, Musgrave, Australia) for acquisition on a Cytoflex flow cytometer (Beckman Coulter Life Sciences ...
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Microbiology 2021Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free RPMI 1640 or DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Cell Biology 2021Quote: LCM samples were washed from lysis buffer by mixing with an equal portion of SPRI bead solution (Beckman Coulter, A63881), incubated 5 min R/T and washed twice with 100% EtOH under magnetic field ...
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bioRxiv - Microbiology 2020Quote: ... The phages were then purified by discontinuous centrifugation in a CsCl gradient (1.33, 1.45, 1.50, 1.70 g/cm3) in TM buffer in Ultra-Clear tubes (Beckman Coulter Inc., Fullerton, CA). Finally ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
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bioRxiv - Microbiology 2022Quote: A CsCl step gradient was prepared by underlaying 30 mL benzonase treated and clarified lysate with 3 mL 1.2 g/L CsCl solution and 3 mL 1.4 g/L CsCl solution made in 30 mM Tris and 100 mM NaCl (TN) buffer in 38.6 mL Ultra-Clear ultracentrifuge tubes (Beckman Coulter). Next ...