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Citations for Beckman :
1401 - 1450 of 2181 citations for 6 4 METHOXY PHENYL 3 METHYL IMIDAZO 2 1 B THIAZOLE 5 CARBALDEHYDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... Cell lysates were subjected to two rounds of centrifugation at 18000 rpm for 5 and 30 mins in a JA-25.50 rotor (Beckman). Protein levels were normalized for coimmunoprecipitation and immunoblot analysis using a Bradford Assay ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Microbiology 2019Quote: ... Gradients were then spun at 38,000 RPM for 2 h in a SW41Ti rotor (Beckman Coulter; 331362) at 4°C ...
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bioRxiv - Pathology 2021Quote: ... BALF (10 μl) was applied to glass microscopy slides using a cytocentrifuge (StatSpin CytoFuge 2, Beckman Coulter). Slides were fixed with 10% neutral buffered formalin ...
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bioRxiv - Microbiology 2022Quote: ... 2 ng of each product were pooled for subsequent cleanup using the AmpureXP PCR cleanup protocol (Beckman). The pooled library was quantified by a picogreen assay and loaded onto an Illumina MiSeq Reagent Kit v3 (600-cycle ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was ultracentrifuged for 2 h at 150,000 x g in an Optima XP centrifuge (Beckman) with a MLA-50 rotor ...
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bioRxiv - Microbiology 2022Quote: ... cells were resuspended in 2% FBS-PBS and analyzed by a CytoFLEX flow cytometer (Beckman Coulter, USA). The final results from three independent experiments were averaged and shown in the histogram.
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bioRxiv - Cancer Biology 2024Quote: ... Cells were then resuspended in PBS + 2% FBS and acquired on a Gallios flow cytometer (Beckman Coulter).
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bioRxiv - Biochemistry 2023Quote: ... The cell culture microplate was centrifuged at 300g for 2 min (Beckman Coulter Allegra X-22R Centrifuge) to bring the cells to the well bottom ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Molecular Biology 2023Quote: ... Reactions were incubated for 2 hr at 60°C and purified by Ampure XP beads (Beckman #A63881). Libraries were PCR amplified in a 30 µL reaction containing 14 µL circularized library ...
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bioRxiv - Biochemistry 2024Quote: ... The supernatant containing the membrane fraction was transferred to Beckman ultracentrifuge tubes and subjected to ultracentrifugation for 2 h at 41,000 r.p.m (Beckman Optima L-100 XP ultracentrifuge ...
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bioRxiv - Molecular Biology 2024Quote: ... and gradients were centrifuged at 35,000 RPM for 2 hours 45 min using a SW41Ti rotor (Beckman). Absorbance was followed at 254 nm ...
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bioRxiv - Biophysics 2024Quote: ... Cells were pelleted from 2 L cultures by centrifugation at 4,785g (5,000 rpm in Beckman JLA-8.100) for 20 min ...
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bioRxiv - Microbiology 2024Quote: ... The filtrate was ultracentrifuged at 200,000 xg for 2 hr (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
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bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Molecular Biology 2019Quote: ... was clarified by centrifugation at 10,000 rpm for 30 min at 4 °C using an SW-28 rotor in an Optima L-90K Ultracentrifuge (Beckman Coulter, USA) and was then ultracentrifuged at 25,000 rpm for 6 h at 4 °C using the same rotor ...
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bioRxiv - Molecular Biology 2019Quote: ... The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Density fractions of 1 ml were collected and fractions 9-10 pooled ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was then ultracentrifuged at 50,000 RPM and 4 °C for 90 minutes using a 70.1 Ti rotor (Beckman Coulter, Brea, CA, USA) with 1 mL 15% sucrose cushion per tube ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 60,000 rpm at 4°C for 2hr in an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The translucent polysome pellets were washed by gently dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
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bioRxiv - Neuroscience 2020Quote: ... and 1.5 ml of this was added to the top of the sucrose gradient and was centrifuged at 69,150 x g for 70 min at 4°C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. # 331362). 1–1.5 ml of the synaptosome layer was collected from the interface of the two sucrose layers ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...
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bioRxiv - Cell Biology 2019Quote: ... The obtained supernatant (containing membrane and cytosolic proteins) was then centrifuged at 180,000 × g for 90 min at 4°C using a TLA-55 rotor (Beckman Coulter, Brea, CA). The obtained pellet (containing intact membranes ...
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bioRxiv - Cell Biology 2019Quote: ... Following centrifugation at 35,000 rpm for 24 hr at 4°C using a MLS-50 Swinging-Bucket rotor (Beckman Coulter, Brea, CA) to sediment protein complexes ...
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bioRxiv - Molecular Biology 2019Quote: ... The DG was centrifuged 18 h at 100,000 g and 4 °C using SW 32.1 Ti rotor (Beckman Coulter, Fullerton, California, USA). Density fractions of 1 ml were collected and fractions 9-10 pooled ...
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bioRxiv - Molecular Biology 2019Quote: ... Stained cells were washed twice in 500ul cold differentiation media and assayed on a 4 laser CytoFLEX S Flow Cytometer (Beckman Coulter, B75408). Data was processed in FlowJo V10 ...
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bioRxiv - Molecular Biology 2019Quote: ... in sucrose cushion tubes and centrifuged for 90 min at 75,000 rpm and 4°C in a TLA120-rotor (Beckman; Indianapolis, IN, USA). Pellets were resuspended in lysis buffer by continuous agitation at 4°C and transferred to non-stick tubes.
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bioRxiv - Microbiology 2020Quote: ... the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter, Indianapolis, IN) to remove unbound stain ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Microbiology 2020Quote: ... were further purified by centrifugation through a 1.2 g/cm3 to 1.6 g/cm3 cesium chloride density gradient at 50,000 RPM for 4 hours at 11 °C in a Beckman SW 65 Ti rotor (Beckman Coulter, Brea, California). Visible “virus bands” in the gradients were collected using a Hamilton syringe and diluted 1:10 in 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... virions were purified from the infected S2 cells by centrifugation at 8,000 RPM for 10 minutes at 4 °C using a Beckman GSA rotor (Beckman Coulter, Brea, California). The supernatant was then collected and centrifuged at 8,000 RPM for 30 minutes at 4 °C using a Beckman GSA rotor ...
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bioRxiv - Immunology 2021Quote: ... Cell debris was removed by centrifugation (45 min at 18,000 rpm, at 4°C; Avanti® J-26xp centrifuge (BECKMAN COULTER®)) ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 x g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter, Brea, CA). The cell pellet was resuspended in 5 ml ice-cold PBS and stored at −80 °C for further use ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45.000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter, Brea, CA). The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...