Labshake search
Citations for Beckman :
1351 - 1400 of 1935 citations for 6H Pyrazolo 3 4 g benzothiazole 2 methyl 8CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... After centrifugation at 15,000 rpm at 4°C for 0.5 h in an JA-30.50 rotor (Beckman), the obtained soluble extract was mixed with 2 ml slurry Ni-NTA beads (30210 ...
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bioRxiv - Biochemistry 2020Quote: The cell lysates obtained after sonication were centrifuged (16000 rpm, JA-17, 45 min, 4 °C, Beckman) to remove unlysed cells and other high molecular weight debris ...
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bioRxiv - Cancer Biology 2021Quote: ... and cell diameters determined using a particle sizing and counting analyser (Multisizer 4 Coulter counter, Beckman Coulter) with Multisizer 4 software.
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Dissecting β-Cardiac Myosin and Cardiac Myosin-Binding Protein C Interactions using a Nanosurf AssaybioRxiv - Biophysics 2022Quote: ... Lysates were centrifuged (176,000g, 4°C, 25 min) in a TLA 100.4 rotor (Beckman Coulter, Brea, CA) and bound to anti-FLAG M2 affinity resin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... The plate was bolted to a Swinging-4-bucket rotor (from a Beckman Coulter, Allegra 6KR centrifuge). This plate+swinging bucket rotor assembly is referred to as the ‘CFM rotor’ or ‘rotor.’ Onboard components were attached to the CFM rotor using nuts and bolts of appropriate dimensions ...
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bioRxiv - Biophysics 2019Quote: ... The lysate was spun for 35 min at 35000 rpm at 4°C (Ti70 rotor, Beckman Coulter) and the supernatant was filtered through a 5 µm syringe filter (Millex ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were cleared by centrifugation (55,000 rpm, 60 min, 4°C, 70 Ti rotor (Beckman Coulter)) ...
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bioRxiv - Cell Biology 2021Quote: ... Cell lysates were cleared by centrifugation (50,000 rpm, 60 min, 4°C, 70 Ti rotor (Beckman Coulter)) ...
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bioRxiv - Biophysics 2020Quote: ... Cells were collected by centrifuging for 10 min at 4 °C at 4,000g (JLA-9.1000, Beckman Coulter). The bacterial pellet was resuspended in 50 mM Tris-HCl pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4 mM DTT)) were added to the flow chambers after centrifugation in an ultracentrifuge (Beckman Airfuge) at 119,000xg for 5 minutes ...
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bioRxiv - Microbiology 2022Quote: ... 4 °C) in an Optima LE-80K ultracentrifuge with a 50.2 Ti rotor (Beckman-Coulter Life Sciences). Supernatants were removed ...
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bioRxiv - Neuroscience 2022Quote: ... Insoluble material was removed by ultracentrifugation (1h, 4°C, 40,000 rpm, Ti70 rotor, Beckman Coulter Cat#337922). The fragments were immobilized onto glutathione Sepharose (Millipore/Cytiva Cat#17-5132-02 ...
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bioRxiv - Physiology 2023Quote: ... and centrifuged at 12,000g for 1 hour at 4°C (SW-41Ti rotor, Beckman Coulter, Pasadena, California). Six or seven fractions were collected from the top ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were harvested by centrifugation at ∼6250g for 15 min at 4°C (Beckman Coulter centrifuge, JLA8.1000).
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then centrifuged at ∼28500g for 1h at 4°C (Beckman Coulter centrifuge, JA-14) and the supernatant incubated for 1h ...
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bioRxiv - Neuroscience 2023Quote: ... the C-tubes were quickly spun in a chilled (4°C) Allegra-30R centrifuge (#B08708, Beckman Coulter) with an SX4400 swinging bucket rotor to collect the samples in the bottom of the tubes ...
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bioRxiv - Biophysics 2023Quote: ... the mixture was centrifugated for 30 min at 12,000 rpm and 4 °C (Beckman J2-21, USA), and iVCL supernatant was collected ...
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bioRxiv - Biophysics 2023Quote: ... and centrifuged at 5000 xg for 30 minutes at 4°C and subsequently at 10000 xg (Beckman Coulter Optima L-90K with a SW40Ti rotor) ...
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bioRxiv - Genomics 2023Quote: ... the C-tubes were quickly spun in a chilled (4°C) Allegra-30R centrifuge (#B08708, Beckman Coulter) with an SX4400 swinging bucket rotor to collect the samples in the bottom of the tubes ...
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bioRxiv - Bioengineering 2024Quote: Microbubble concentration and number- and volume-weighted size distributions were measured with a Multisizer 4 (Beckman Coulter). Microbubble concentration (ci ...
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bioRxiv - Plant Biology 2024Quote: ... The cell concentration was measured in triplicate with a particle counter (Multisizer 4 Counter, Beckman Coulter, USA) after each disruption cycle ...
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bioRxiv - Molecular Biology 2024Quote: ... placed at the bottom of the tube and centrifuged at 5,000x g for 15 min at 16 °C in a swinging bucket rotor (Beckman Coulter, JS-5.3 or JS-7.5). The coverslips were recovered and fixed with pre-chilled methanol (–20 °C ...
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bioRxiv - Biochemistry 2024Quote: ... NaCl was then added to 1 M and the lysed solution was centrifuged at 20,000 g at 1°C for 45 minutes (Beckman Coulter J2-21, JA 20 rotor). Tween-20 was added to the supernatant to 0.1% (v/v ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by centrifugation at 100,000 ×g for 65 min using the SW40 Ti rotor in an Optima™ XPN-80 ultracentrifuge (Beckman Coulter Inc., Brea, United States). The EV pellets obtained from the 100,000 ×g spin of human neutrophil-derived EVs (HunEVs ...
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bioRxiv - Biophysics 2024Quote: ... The EVs were then purified by ultracentrifugation: the pellet was retrieved after a run at 100,000 g for 90 min (Beckman Coulter Max XP, rotor MLA-50) and resuspended in low volume (~150µL ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Debris was clarified by centrifuging at 18,000 ×g followed by fractionation on a discontinuous sucrose gradient (10-50 %) by ultracentrifugation at 39,000 rpm for 3 h in an SW41-Ti rotor (Beckman coulter). Monosome fractions were collected by pumping of Fluorinert FC-40 and using a fraction collector by measuring UV trace ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradient was formed by adding 3 mL of the iodixanol solutions to a 17 mL polypropylene tube (Beckman Coulter). Ultracentrifugation was performed using a SW Type 32.1 Ti rotor in Optima XPN-100 Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Genomics 2019Quote: ... Samples were size selected using a 0.5X Ampure XP cleanup to remove large fragments followed by 3 consecutive 1.5X Ampure XP (Beckman Coulter # A63880) bead cleanup followed by a final 1.2X Ampure XP cleanup to completely remove all residual primers and resuspended in a final volume of 20 µL EB using the manufacturer’s recommended protocol.
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bioRxiv - Cell Biology 2019Quote: ... Monosomes were isolated by sucrose gradients (10-50%) and centrifuged at 40,000 rpm for 3 hours in a SW41 rotor (Beckman Coulter). The extracted RNA was size-selected from 15% denaturing PAGE gels ...
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bioRxiv - Cancer Biology 2020Quote: The barcode split of the cDNA amplification reaction (from 10x Single Cell 3’ v3 Step 2.2) was purified via 1.2x SPRI Select (Beckman Coulter #B23317). cDNA products were eluted in 40 µL of EB ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2021Quote: 3 mL of pseudoparticles were pelleted using a TLA-55 rotor with an Optima-MAX-E ultracentrifuge (Beckman Coulter, Inc.) for 2 hours at 42,000 rpm at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Supernatants (3 ml) were loaded on the top of step gradients preformed in polyallomer tubes (14 by 89 mm, Beckman), containing 2 ml each of 1.82 ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.3 M, 2.5 M) as indicated in the method (Vella et al., 2017) in ultra-clear SW40Ti tubes (Beckman Coulter). The sucrose gradients were centrifuged at 200,000 x g avg at 4°C for 173 min using a SW40Ti rotor (Beckman Coulter) ...
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bioRxiv - Microbiology 2020Quote: ... was overlaid on 25% sucrose in PBS and centrifuged at 26,000 rpm for 3 h at 4C using SW28 rotor (Beckman Coulter). Pellets were dissolved in PBS overnight at 4C and 1/3 of used for RNA was extraction using Macherey Nagel Virus RNA isolation kit without carrier RNA according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2020Quote: ... Samples are visualized on Cytoflex S, 3 lasers (488 nm, 405 nm and 638 nm) and 13 detectors (Beckman Coulter). Live cells were gated based on side scatter and forward scatter ...
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bioRxiv - Molecular Biology 2020Quote: ... and the cleared supernatant was loaded on a 3 to 30% iodixanol gradient (OptiPrep) and ultracentrifuged for 5h at 36,000 rpm in an SW60 rotor (Beckman Coulter). Fractions were collected manually through aspiration and RNA or proteins were isolated for further analysis.
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bioRxiv - Microbiology 2020Quote: ... The filtered supernatant was then spun through a 20% sucrose cushion at 48,000 rpm for 3 hours in a SW55Ti rotor (Beckman Coulter). The SVP pellets were resuspended in a 4X non-reducing buffer (Alfa Aesar ...
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bioRxiv - Molecular Biology 2019Quote: ... Rnase-free sucrose) and gradients were centrifuged at 35,000 rpm for 3 hours 20 minutes using a SW41Ti rotor (Beckman Coulter). Absorbance was followed at 254 nm ...
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bioRxiv - Microbiology 2022Quote: ... HUVECs or LECs were infected for 3 days and the GFP positive population was quantified by CytoFLEX S (Beckman Coulter). Here ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2024Quote: ... The flow cytometry analysis of the bacterial cells present in the suspension was performed using a Cytoflex flow cytometer (CytoFLEX 3; Beckman) as previously described (Supplementary Fig ...
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bioRxiv - Neuroscience 2024Quote: ... at a ratio of 1:2.3 before layering on 3 ml of SCB in a 12 ml Thinwall Ultra-clear centrifuge tubes (Beckman Coulter). Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... The reactions were then layered on 100 μL of 32% sucrose cushion and centrifuged at 42,800 rpm for 3 hours in a Beckman TLA 100 rotor using the Optima-Max TL ultracentrifuge (Beckman Coulter). After centrifugation ...
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bioRxiv - Cancer Biology 2020Quote: ... BSA-coated ultracentrifugation tubes prefilled a with 10 ml sucrose buffer cushion were carefully overlaid with the dissolved nuclei pellet and centrifuged for 60 minutes at 13,000 g (Beckman, Brea, CA, Avanti S-25, JA25.50 rotor). The supernatant was discarded and the isolated nuclei were dissolved in 1 ml nuclei buffer (0.44 M sucrose ...
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bioRxiv - Microbiology 2023Quote: ... briefly mixed and equilibrated for ∼30 min (Albumax equilibration was conducted in a 5% CO2 incubator to maintain pH 7.4) after which they were subjected to ultracentrifugation at 37°C using a sealed rotor (Beckman Rotor type 42.2 Ti; 223,000 x g) for 4.2 h to separate proteins ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...