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Citations for Beckman :
1301 - 1350 of 1463 citations for 3RS 4 Dimethylamino 3 methyl 2 2 diphenylbutanenitrile Isodidiavalo since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2024Quote: ... The combined supernatants were centrifuged at 100,000 g for 40 min (4°C) on a TL110 rotor (Beckman Coulter, Paris, France) and the pellet was suspended in the same buffer ...
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bioRxiv - Molecular Biology 2024Quote: ... The tube was centrifuged at 38,000 x g for 21-24 h at 4°C using the SW40T1 rotor in the Optima L-60+ Ultracentrifuge (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Microbiology 2024Quote: ... This sample was then ultracentrifuged at 150,000 g for 2.5 h at 4 °C using a Type 70 Ti or Type 45 Ti rotor in an Optima XE Ultracentrifuge (Beckman Coulter). The translucent VLP pellet formed by this process was resuspended in 200 μl PBS buffer ...
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bioRxiv - Immunology 2024Quote: ... Centrifugation was performed (30 min, 800 g, 4 °C) in a swinging bucket on a Beckman Coulter JS-13.1 rotor (Beckman Coulter, USA). Hemocytes concentrated at each density interface were collected separately with a 70 mm long 20Gx2.75” needle mounted on a 1 mL syringe ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 100µL of PBS and stored at 4°C in the dark until samples were analyzed on a CytoFLEX LX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo® software.
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bioRxiv - Biochemistry 2024Quote: ... Non-solubilized materials were removed by centrifuging the sample at 41,000 r.p.m for 1 h at 4 °C using rotor type 45 Ti in the OptimaTM L-100 XP Ultracentrifuge (BECKMAN COULTER). The resulting supernatant containing the solubilized LysP-GFP was incubated with Ni2+-NTA resin (Qiagen ...
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bioRxiv - Microbiology 2024Quote: ... and labeled intracellularly for the structural HIV-1 p24 protein with the anti-p24 clone KC57 antibody (30 min RT followed by 30 min 4°C, Beckman Coulter). HIV-1 RNA probing was performed using the PrimeFlow RNA Assay (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... Samples were balanced and centrifuged at 42,000 rpm for 35m at 4°C in a SW55Ti rotor and buckets with tube adaptors (Beckman REF: 356860). The supernatant was then concentrated to 80μl by spinning through Amicon 3kDa MWCO concentrator units at 14,000xg for 15-30m at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The total number of microglia (CD11b+ CD45+ cells) in the L3/4 spinal cord or SDH was analyzed using CytoFLEX SRT (Beckman Coulter) and FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2024Quote: ... The obtained culture was pelleted at low speed (7000 rpm for 10 minutes at 4 0C in a Beckman Coulter centrifuge). Harvested cells were resuspended (1 g of cell pellet per 10 mL ...
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Sense codon-misassociated eRF1 elicits widespread ribosome stalling and induction of quality controlbioRxiv - Molecular Biology 2024Quote: ... The mixture was layered over 900 μl of 1 M sucrose cushion 46 and ultracentrifuged at 100,000 rpm ( 543,000 × g) at 4°C for 1 h using a TLA-110 rotor and an Optima MAX-TL Ultracentrifuge (both Beckman Coulter).
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bioRxiv - Microbiology 2024Quote: ... the supernatant was filtered through 0.45-μm syringe filters and ultracentrifuged at 100,000 ×g for 1 h at 4 °C (MLA-50 fixed angle rotor, Beckman Coulter, Germany). The supernatant was discarded ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation at 60,000 x g for 20 min at 4°C in a swing-out rotor (MLS-50, Optima Max-XP, Beckman Coulter), the mitochondrial fraction localized in the interphase between the two sucrose layers was carefully removed with a syringe ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were centrifuged at 4°C for 24 h at 274,400 x g in a SW-40Ti rotor (Beckman Coulter Inc.). To remove KBr ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in PBS and stored in the dark at 4°C until analyzing them with a Navios flow cytometer (Beckman Coulter). At least 10’000 living cells were measured for each sample ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Bioengineering 2024Quote: ... using C4F10-saturated PBS until the subnatant was clear after which MBs were counted using a Coulter Counter Multisizer 3 (50 µm aperture tube, Beckman Coulter, Mijdrecht, the Netherlands). Next ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Biophysics 2020Quote: ... The lysates were clarified by centrifugation (16,000 rpm for 1 h at 4°C, rotor JA 25.50, Beckman Coulter, Brea, California, USA). The supernatant was incubated with amylose resin for 1 h at 4°C and loaded onto a 20 ml chromatography column (Bio-Rad ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Systems Biology 2020Quote: ... Next the cultures were diluted 1:40 with 0.5M NaCl and immediately measured in Multisizer™4 COULTER COUNTER® (Beckman Coulter) (Coulter ...
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...
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bioRxiv - Plant Biology 2021Quote: ... The resulting slurry was centrifuged for 15 min at 9000 rpm at 4 °C using a JA14 rotor (Beckman Coulter, Brea, CA). The supernatant was filtered with a Miracloth and kept on ice ...
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bioRxiv - Biochemistry 2021Quote: ... Lentivirus from the cell supernatant was collected 24h later by ultracentrifugation with a Sw28 rotor (Beckman Coulter; 22,000 rpm, 2h, 4°C). Finally ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting supernatant was then ultracentrifuged at 50,000 RPM and 4 °C for 90 minutes using a 70.1 Ti rotor (Beckman Coulter, Brea, CA, USA) with 1 mL 15% sucrose cushion per tube ...
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bioRxiv - Cell Biology 2021Quote: ... The samples were centrifuged at 60,000 rpm at 4°C for 2hr in an SW65 Ti ultracentrifuge rotor (Beckman Coulter, California, USA). The translucent polysome pellets were washed by gently dispensing and removing 500 µl of ice-cold RNase-free water ...
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bioRxiv - Microbiology 2020Quote: ... RNA was pelleted by centrifugation for 24 h at 4 °C and 104.000 g in a SW28.1 swing out rotor (Beckman Coulter, Brea, CA, USA). RNA was taken up in 100 µl H2O and 500 µl TS buffer was added ...
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bioRxiv - Molecular Biology 2020Quote: ... the serum was centrifuged (200,000xg, at 4°C for 18 hours) using a Beckman Coulter Optima XE-100 Ultracentrifuge (Beckman Coulter, CA, USA), with a SW41Ti rotor (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... The crude viral lysate was purified with two rounds of CsCl gradient ultracentrifugation at 100,000×g and 4°C for 23 h using 13.5-mL Quick-Seal tubes and a NVT65 rotor (Beckman Coulter, Brea, CA). Each tube was placed gently and 500– 750 µL of white turbid bands were collected ...
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bioRxiv - Molecular Biology 2022Quote: ... were pre-clarified to remove aggregate proteins by spinning at 65,000 rpm for 30 min at 4°C in a TLA100 rotor (Beckman Coulter, Brea, CA) before incubating with F-actin ...
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bioRxiv - Microbiology 2022Quote: ... virions collected over the three passages were concentrated on 20% sucrose cushions (prepared in 1X phosphate buffered saline (PBS)) by ultracentrifugation (28000 rpm, 4°C, 90min, Beckman SW41 rotor). Genomic RNA was isolated from pelleted virions using Trizol per manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... A 5 to 30% linear sucrose gradient was formed above the homogenate and centrifuged at 40,100 rpm and 4° C for 16 hours in a SW55 rotor using an OptimaTM LE-80K Ultracentrifuge (Beckman Coulter, Pasadena, CA). Fourteen 360 μl fractions were collected from the top and analyzed by SDS–PAGE/Western blot with an equal loading volume ...
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bioRxiv - Neuroscience 2020Quote: ... and 1.5 ml of this was added to the top of the sucrose gradient and was centrifuged at 69,150 x g for 70 min at 4°C (SW 41 Ti Swinging-Bucket Rotor, Beckman Coulter, Cat. # 331362). 1–1.5 ml of the synaptosome layer was collected from the interface of the two sucrose layers ...
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bioRxiv - Biophysics 2020Quote: ... cell lysate was centrifuged by using The Beckman Optima™ L-80 XP Ultracentrifuge at 40000 rpm for 30 minutes at 4°C by using Ti45 rotor (Beckman, USA). After ultracentrifugation the pellet which contains membranes and insoluble debris was discarded and clear supernatant applied to nickel affinity chromatography by using a Ni-NTA agarose resin (QIAGEN ...
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bioRxiv - Microbiology 2020Quote: ... the Exos were washed three times with PBS before ultracentrifugation at 100,000□x□g for 20□min at 4□°C using Beckman L7-80 and rotor SW41 (Beckman Coulter, Indianapolis, IN) to remove unbound stain ...
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bioRxiv - Biochemistry 2020Quote: ... which was then centrifuged at 160,000×g at 4°C for 16 h in a Beckman MLS–50 rotor (Beckman–Coulter, CA, USA), and 24 fractions were collected ...
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bioRxiv - Microbiology 2020Quote: ... were further purified by centrifugation through a 1.2 g/cm3 to 1.6 g/cm3 cesium chloride density gradient at 50,000 RPM for 4 hours at 11 °C in a Beckman SW 65 Ti rotor (Beckman Coulter, Brea, California). Visible “virus bands” in the gradients were collected using a Hamilton syringe and diluted 1:10 in 10 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... virions were purified from the infected S2 cells by centrifugation at 8,000 RPM for 10 minutes at 4 °C using a Beckman GSA rotor (Beckman Coulter, Brea, California). The supernatant was then collected and centrifuged at 8,000 RPM for 30 minutes at 4 °C using a Beckman GSA rotor ...
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bioRxiv - Plant Biology 2020Quote: ... Double size selection was carried out by adding 50 μL of a 1:4 (0.5X) mixture of AMPure XP Beads (Beckman Coulter, Brea, USA) to PEG buffer (20% PEG w/v ...
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bioRxiv - Immunology 2021Quote: ... Cell debris was removed by centrifugation (45 min at 18,000 rpm, at 4°C; Avanti® J-26xp centrifuge (BECKMAN COULTER®)) ...
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bioRxiv - Cell Biology 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 x g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter, Brea, CA). The cell pellet was resuspended in 5 ml ice-cold PBS and stored at −80 °C for further use ...
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bioRxiv - Cell Biology 2020Quote: ... and centrifuged at 45.000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter, Brea, CA). The cleared cell lysate was incubated for 2 h at 4 °C in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 100 000 × g for 60 min at 4°C using rotor Ti-70 in an Optima LE-80K Ultra Centrifuge (Beckman Coulter, Australia). The harvested EV pellet was resuspended in 1 mL RNase-free Phosphate buffered saline (RNase-free PBS ...
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bioRxiv - Neuroscience 2021Quote: ... Lentiviruses were purified by overlaying the virus containing media over a 20% sucrose solution before ultracentrifugation at 26,000 rpm for 90 minutes at 4 °C in a SW28Ti swinging-bucket ultracentrifuge rotor assembly in an Optima XPN-80 Ultracentrifuge (Beckman Optima Ultracentrifuge) and the viral pellet was resuspended in AimV™ media.
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bioRxiv - Cell Biology 2020Quote: ... supernatants were centrifuged at 10,000 x g for 30 min at 4°C and the supernatants were transferred to ultracentrifuge tubes (Beckman Coulter, Brea, CA) and diluted with phosphate-buffered saline (PBS) ...
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bioRxiv - Microbiology 2020Quote: ... All bacteria were harvested by centrifugation at 4000 rpm and 4°C in a JS 4.0 swinging bucket rotor (Beckman Coulter, Brea California) and immediately processed as described below.
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bioRxiv - Microbiology 2021Quote: ... density gradient (35 to 0% in PBS) and ultracentrifuged at 200,000 × g for at least 12 hours at 4 °C (Beckman Optima XL-100K). The corresponding clear yellow band was collected ...