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Citations for Beckman :
1201 - 1250 of 1483 citations for 5 Bromo 2 Tert Butyldimethylsilyl 4 Methylthiazole since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... was loaded on top of the gradient and spun at 4°C for 18 hr at 30,000 rpm (Beckman, Rotor SW-41Ti). Samples were fractionated with absorbance optics (λ = 260 nm ...
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bioRxiv - Neuroscience 2024Quote: ... The PNS- Nycodenz gradient was centrifuged for 1 h at 141,000 x g at 4°C (46,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... This gradient was centrifuged for 30 minutes at 72,000 x g at 4°C (33,000 rpm, TLS-55 rotor, Beckman Coulter Optima TLX Ultracentrifuge). After centrifugation ...
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bioRxiv - Microbiology 2024Quote: ... The top agar was then scraped off the LB agar and centrifuged for 26,000 x g at 4°C for 1 hour (Beckman Coulter JA-17). The supernatant was then removed ...
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bioRxiv - Microbiology 2024Quote: ... Crude ribosomes were separated from other low molecular weight cellular contents by centrifugation at 35000 rpm (126,000g) for 1 hour at 4 °C (Beckman rotor Type 70Ti). The crude ribosome pellet was resuspended in 10 mL HMA-10 buffer and layered on top of a 10 mL 32% sucrose solution in HMA-10 buffer and centrifuged for 16 hours at 37,000 rpm (140,000g ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was segregated subsequent to centrifuging the brain tissue homogenate for 1 h at 100,000g and 4°C utilising an ultra-high-speed centrifuge (Beckman, Optima MAX-XP). The tissue residue was then reprocessed ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: The number of μPL per PVA template and size distribution were derived via a Multisizer 4 COULTER particle counter (Beckman Coulter, CA). Briefly ...
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bioRxiv - Neuroscience 2024Quote: ... The suspension was then centrifuged at 2500 × g for 30 min at 4 °C and the supernatant was transferred to an ultra-centrifugation tube (Quickseal Tubes, Beckman Coulter #342414). AAV purification was performed by ultra-centrifugation over a discontinuous Iodixanol density gradient (OptiPrep Density Gradient Medium ...
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bioRxiv - Plant Biology 2024Quote: ... The fluid was centrifuged at 1,000 x g for 10 minutes at 4 C using an Allegra X-30R centrifuge (Beckman Coulter Life Sciences). The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences) ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting supernatant was centrifuged at 200,000 x g for 30 minutes at 4 °C using a Beckman Optima Ultracentrifuge with TLA110 rotor (Beckman Coulter Life Sciences). The pellet was resuspended in 30 µL of cold ddH20 and stored in −80 °C until further use.
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bioRxiv - Biochemistry 2024Quote: ... Purified nucleosome arrays were subsequently isolated on 5-40% w/v sucrose gradients in 0.5X TE by centrifuging the gradients at 39,000 rpm and 4°C for 8 h in a SW41 Ti rotor (Beckman Coulter, Brea, CA). After centrifugation ...
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bioRxiv - Cell Biology 2024Quote: ... The crude pellets from MSC-CM and HDF-CM after the first-round 100,000 × g ultracentrifugation were washed with PBS at 100,000 × g and 4 ℃ for 45 min using Optima™ MAX-TL Ultracentrifuge (Beckman Coulter, USA) and TLA-120.2 Rotor (Beckman Coulter ...
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bioRxiv - Biophysics 2020Quote: ... lysate was centrifuged at 5 °C with 13000 rpm for 15 min by Avanti J-E Ultra Centrifuge (Beckman Coulter). Supernatant from centrifugation was filtered with 0.45 μm SFCA syringe filters (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and overlaid with 6 ml of 30% and 3 ml of 5% sucrose (w/v) followed by centrifugation at 32,000 rpm in SW41 rotor (Beckman Coulter) for 20 h ...
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bioRxiv - Molecular Biology 2020Quote: ... Four mL of each density (3.5 mL for the 5% density) was stacked into an open polyallomer tube (337986, Beckman Coulter) using a Tecan freedom EVO ...
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bioRxiv - Biophysics 2022Quote: ... The cell suspension was centrifuged for 5 min at 2000 g (Centrifuge Beckman Coulter Allegra X-15-R, Rotor Sx4750A)) and 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... 5 × 105 additional cells were immediately processed for far red signal analysis by flow cytometry (Gallios, Beckman Coulter, Brea, USA) (D0) ...
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bioRxiv - Neuroscience 2020Quote: ... reverse 5’-CTTA TTAGTCAGTCAGAAACAAC-3’).ThePCR amplicon was purified using RNAse-free SPRI beads (Agencourt RNAclean XP, Beckman-Coulter A63987) and then used for in vitro transcription with the HiScribe T7 ARCA mRNA Kit (with tailing ...
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bioRxiv - Microbiology 2020Quote: ... for 5 minutes using a FIBERLite F10BCI-6×500y rotor (Piramoon Technologies) in an Avanti JXN-30 centrifuge (Beckman Coulter). The clarified cell lysate was further centrifuged in a JS-24.38 rotor (Beckman Coulter ...
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bioRxiv - Microbiology 2020Quote: ... the 96-well plate was centrifuged at 1,000 RPM for 5 min using an Allegra 25R model centrifuge (Beckman Coulter) with inserts for 96-well plates ...
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bioRxiv - Microbiology 2020Quote: ... At the end of each selection cells were pelleted from the media by centrifugation 200 x g for 5 min in an Allegra centrifuge (Beckman) and supernatant was transferred for storage at −80C ...
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bioRxiv - Microbiology 2022Quote: ... and layered onto a preformed 11 mL 5% to 15% (wt/vol) Ficoll gradient in a 13.2 mL thin-wall polyallomer ultracentrifuge tube (Beckman Coulter). Gradients were centrifuged at 12,000 rpm for 2 h at 4°C in an SW41 Ti swinging-bucket rotor in a Sorvall Discovery SE ultracentrifuge ...
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bioRxiv - Immunology 2022Quote: ... 1.5 μl of PI was also added and samples were run on the Gallios™ Flow Cytometer (Beckman Coulter, Inc). Data was analysed offline using Kaluza® Flow Analysis software version 2.1a (Beckman Coulter Inc. ...
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bioRxiv - Immunology 2021Quote: ... subsequently overlaid onto 5 mL of 20% sucrose and ultracentrifuged at 24,000 rpm for 2.5 hrs (Beckman XPN80, SW32.Ti). The viral pellet was resuspended in PBS.
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bioRxiv - Microbiology 2021Quote: ... bacterial cells were washed in PBS and diluted 1:5 before acquisition on a CytoFLEX benchtop flow cytometer (Beckman Coulter). For each sample ...
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bioRxiv - Cancer Biology 2024Quote: ... The fragments were repaired the 3’and 5’overhangs using End repair mix (component of SureselectXT) and purified using Agencourt AMPure XP Beads (Beckman). The purified fragments were added with ‘A’ tail using A tailing Mix (component of SureSelectXT ...
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bioRxiv - Microbiology 2023Quote: ... Sample analysis was performed by mixing 5 µl of fluorescent vesicles with 20 µl of Flow-Count Fluorospheres (Beckman Coulter) in a final volume of 200 µl of PBS to determine absolute counts ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were then loaded onto a 5-50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... Lysed cells were centrifuged and the viscous supernatant was immediately loaded on a 5-24% (w/v) sucrose density gradient and centrifuged in SW 32 Ti rotor (Beckman) overnight ...
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bioRxiv - Microbiology 2023Quote: Human bladder cell line PD07i was cultured at 37°C with 5% CO2 to confluency as previously described and counted using a Coulter Counter (Beckman) to a minimum of 1×105 cells/mL ...
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bioRxiv - Molecular Biology 2023Quote: ... The lysates were sheared with a 25-gauge 5/8 needle and loaded onto CsCl solution (150% w/v) for ultracentrifugation in NVT 65.2 rotor (Beckman coulter) at 42,000 rpm for 20 hours at 4°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Concentrated chromatin samples were loaded on 11 mL gradients of 5-25% sucrose in TE buffer and centrifuged in a preparative ultracentrifuge with a SW41 Ti rotor (Beckman) for 11 hrs ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were centrifuged at 1500 g for 5 min in an F2402 rotor in a GS15R table-top centrifuge (Beckman) and resuspend with water to wash the cells ...
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bioRxiv - Biophysics 2023Quote: ... Each 300μl dialysate was mixed with 300μl of 80% (w/v) Nycodenz dissolved in reconstitution buffer and then divided equally into two 5 x 41 mm ultracentrifuge tubes (Beckman). Then ...
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bioRxiv - Cancer Biology 2024Quote: ... The lysates were then loaded onto a 5–50% sucrose density gradient and centrifuged in an SW41 Ti rotor (Beckman) at 36,000 rpm for 2 h at 4 °C ...
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bioRxiv - Developmental Biology 2024Quote: ... The samples then underwent a second Zymo DNA Clean & Concentrator-5 purification and were eluted in 30µl of elution buffer prior to a 1.2X AMPure XP bead (Beckman Coulter; A63881) cleanup ...
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bioRxiv - Genetics 2021Quote: ... and samples were evaluated 2 minutes later with a Cytomics FC500 flow cytometer (Beckman Coulter, Brea, CA, USA) AO was excited with a 488 nm argon laser ...
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bioRxiv - Genomics 2020Quote: ... The PCR products were purified by using 1.3X Agencourt AMPure XP beads (2 or 3 cycles; Beckman Coulter) and evaluated by using an Agilent High Sensitivity DNA Kit on an Agilent 2100 Bioanalyzer (Agilent) ...
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bioRxiv - Cancer Biology 2020Quote: ... concentrated for 2 h by ultracentrifugation in a swing-out rotor SW-32Ti (Beckman Coulter, Brea, US-CA) at 26000 g ...
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bioRxiv - Microbiology 2023Quote: ... The filtrate was subjected to ultracentrifugation at 200,000xg for 2 h (Optima L-100 XP ultracentrifuge; Beckman Coulter). Supernatants were discarded ...
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bioRxiv - Microbiology 2024Quote: ... (The filtrate was subjected to ultracentrifugation at 100,000g for 2 h (Optima L-90 K ultracentrifuge; Beckman Coulter). The supernatant was discarded and the pellet was washed with sterile PBS and filtered through a sterile 0.2 μm-pore polyvinylidene difluoride (PVDF ...
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bioRxiv - Immunology 2023Quote: ... CD27 and BA.1 or BA.2 were sorted using a MoFlo Astrios EQ Cell Sorter (Beckman Coulter). FACS data were collected by Summit 6.0 (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: ... Lentiviral particles were then concentrated by centrifugation for 2 hours at 20,000 rcf (Beckman Coulter, Optima L-100K) in round bottom polypropylene tubes (Beckman Coulter ...
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bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
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bioRxiv - Physiology 2024Quote: ... was analysed via a sequential 2-step immune-enzymatic assay (inter-assay CV 5.2-7.8%) according to manufacturer’s instructions (Beckman Coulter) and follicle stimulating hormone (FSH ...
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bioRxiv - Immunology 2024Quote: ... The whole cell lysate was sonicated for 2 min and the cell lysate was centrifuged for 60 min at 20,000 rpm (JA 25.50 rotor, Beckman). The recombinant P72 protein in the supernatant were collected and applied to the anti-HA Magnetic Beads (MCE ...
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bioRxiv - Cancer Biology 2024Quote: ... 2 μl of the sample pre- and post-bead clean-up (SPRIselect beads, Beckman Coulter, cat. no. B23317) were run in the TapeStation D1000 as a quality control to check peak size ...
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bioRxiv - Cell Biology 2024Quote: ... MKs were subsequently fixed with 2% paraformaldehyde and activation measured using the CytoFLEX flow cytometer apparatus (Beckman Coulter). Normalized mean florescence intensity (MFI ...
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bioRxiv - Microbiology 2020Quote: ... by ultra-centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for two hours at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated off and the pellets were re-suspended in PBS (pH 7.4) ...
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bioRxiv - Microbiology 2020Quote: ... by centrifugation in a Beckman L7-65 ultracentrifuge at 25,000 rpm for 2h at 4°C using a Beckman SW28 rotor (Beckman Coulter, Brea, CA, USA). Supernatants were aspirated and the pellets were re-suspended in PBS (pH 7.4) ...