Labshake search
Citations for Beckman :
1101 - 1150 of 1189 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Genomics 2019Quote: ... selecting for a mean fragment length of 450 bp with the SPRIselect reagent kit (Beckman Coulter, Krefeld, Germany). A mate pair library with an insert size of 5 kb was created with the Nextera Mate Pair Sample Prep Kit (Illumina ...
-
bioRxiv - Cancer Biology 2020Quote: ... then genomic DNA was isolated from cells using the Agencourt DNAdvance DNA isolation kit (Beckman Coulter; Cat#: A48705). All LentiCRISPR target sites (except for those targeting Trp53 ...
-
bioRxiv - Microbiology 2020Quote: RNA was extracted from 200µl sample using RNAdvance Viral kit and the Biomek i7 Automated Workstation (Beckman Coulter) and eluted with 50µl H2O.
-
bioRxiv - Genetics 2019Quote: RNA was extracted from dissected brains or heads using Agencourt RNAdvanced Tissue Kit (Beckman Coulter, Brea, CA: A32645) following the standard protocol but using one fourth of all volumes ...
-
bioRxiv - Microbiology 2021Quote: ... 50μl of heparin-blood was used for trucount analysis with the non-centrifugation PerFix-NC kit (Beckman Coulter). Cells were first stained extracellular with α-CD4-APC (02 ...
-
bioRxiv - Developmental Biology 2020Quote: ... GEMs were harvested and the cDNAs were amplified and cleaned up with SPRIselect Reagent Kit (Beckman Coulter, B23318). Indexed sequencing libraries were constructed using Chromium Single Cell 3’ Library v2 (10X Genomics ...
-
bioRxiv - Immunology 2021Quote: ... Stimulated cells were fixed and permeabilized with the Foxp3 staining kit (eBioscience) or IntraPrep Permeabilizaton Reagent (Beckman Coulter) and then stained with combinations of Foxp3 (FJK-16s ...
-
bioRxiv - Cell Biology 2022Quote: Total RNA of Caco-2 cells and human ileum organoids was extracted using RNAdvance Tissue kit (Beckman Coulter) and total RNA of mouse organoids was isolated using Quick-RNA Miniprep Plus Kit (Zymo Research) ...
-
bioRxiv - Immunology 2022Quote: ... The occult blood in mouse fecal samples was detected using Hemoccult Test Kit (Beckman Coulter Inc, Fullerton, CA).
-
bioRxiv - Genomics 2023Quote: ... The fragmented aRNA was size-selected with 0.8x AMPure RNA beads (RNAClean XP Kit, Beckman Coulter, Cat. # A63987) and eluted in 18 μL nuclease-free water ...
-
bioRxiv - Neuroscience 2023Quote: RNA was extracted from wild-type mouse livers using the Agencourt RNAdvance Tissue kit (Beckman Coulter, Brea, CA), and cDNA was synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
-
bioRxiv - Genomics 2024Quote: ... Samples too diluted to be compatible with the kit were first concentrated using AMPure Beads (Beckman Coulter, #A63880) and re-quantified ...
-
bioRxiv - Molecular Biology 2024Quote: ... DNA was isolated from formalin-fixed paraffin embedded (FFPE) tissue sections using a FormaPure kit (Beckman Coulter, C16675). ddPCR with probes to detect the GNAQ R183Q mutation (Table S1 ...
-
Definition and Signatures of Lung Fibroblast Populations in Development and Fibrosis in Mice and MenbioRxiv - Cell Biology 2020Quote: ... GEMs were collected and the cDNA was amplified and purified with SPRIselect Reagent Kit (Beckman Coulter, Brea, CA, USA). Indexed sequencing libraries were constructed using Chromium Single-Cell 3′ Library Kit for enzymatic fragmentation ...
-
bioRxiv - Neuroscience 2020Quote: ... 17β-estradiol levels were determined in plasma using a commercially available radioimmunoassay kit (ultrasensitive estradiol RIA, Beckman Coulter, DSL4800). All samples were run in duplicate ...
-
bioRxiv - Genomics 2021Quote: ... Library cleanup was done using Zymo Research DNA Clean & Concentrator kit followed by AMPure XP bead (Beckman Coulter #A63880) size selection ...
-
bioRxiv - Immunology 2021Quote: ... Colon length was measured and blood in the stool was assessed using a hemoccult blood test kit (Beckman Coulter). Colon sections were fixed in 10% phosphate buffered formalin ...
-
bioRxiv - Cell Biology 2020Quote: ... GEMs were broken and the single strand cDNA cleaned up with DynaBeads (Fisher) and SPRIselect Reagent Kit (Beckman Coulter). cDNA was amplified ...
-
bioRxiv - Immunology 2022Quote: The TCR V beta repertoire kit contained antibodies to over 76% of V βeta families (Beckman Coulter, Fullerton, CA). The cells were stained with these antibodies in combination with tetramers or dextramers for 20 minutes at RT to determine the TRBV repertoire of antigen specific cells ...
-
bioRxiv - Cancer Biology 2023Quote: ... ligated with index adaptors and size selected with cleanups between each step using the SPRIselect Reagent kit (Beckman Coulter). Quality control and quantification of the resulting dual-indexed barcoded libraries was performed with Agilent TapeStation and by qPCR (NEBNext Library Quant Kit for Illumina ...
-
bioRxiv - Microbiology 2023Quote: ... The libraries construction was carried out with Agilent’s SureSelect Strand-Specific RNA Library Preparation Kit followed by size selection with AMPure XP beads (Beckman Coulter ...
-
bioRxiv - Immunology 2023Quote: ... Preamplification was then performed on the products which were subsequently purified using the SPRIselect Reagent Kit (Beckman Coulter, B23318). The paired V(D)J BCR sequences were enriched with 10X BCR primers ...
-
bioRxiv - Microbiology 2024Quote: Total RNA was extracted from persistently BoDV-2-infected Vero cells using Agencourt® RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex system (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2020Quote: ... Serum 17β-estradiol levels in female rats and serum testosterone levels in male rats were assayed using commercially available radioimmunoassay (RIA) kits from Beckman Coulter (Brea ...
-
bioRxiv - Genomics 2020Quote: Pieces of caudal fin sampled from those 1,152 fish were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdavance kit from Beckman Coulter following manufacturer instructions ...
-
bioRxiv - Cancer Biology 2020Quote: ... isotype controls for intracellular stains and subjected to post-acquisition compensation using VersaComp Antibody Capture Bead Kit (Beckman Coulter #B22804). Data was analyzed using Kaluza Software (Beckman Coulter) ...
-
bioRxiv - Plant Biology 2021Quote: ... The pool was then purified using the solid phase reversible immobilization (SPRI) Agencourt AMPure XP kit (Beckman Coulter, Milan, Italy).
-
bioRxiv - Microbiology 2021Quote: Total RNA was isolated from Vero E6 and Caco-2 cells 48 hours after infection using RNAdvance Viral Kit (Beckman) using a KingFisher and RNeasy Mini Kit (Qiagen) ...
-
bioRxiv - Developmental Biology 2022Quote: ... TH concentrations are measured by a medical laboratory in Perpignan (Médipole) using an ELISA kit (Access Free T3, T4, Beckman Coulter).
-
bioRxiv - Plant Biology 2019Quote: ... were combined in a barcode-wise manner and purified using the Agencourt AMPure XP kit (Beckman Coulter, Brea, United States) with a ratio of 0.7 mL AMPure XP beads per 1 mL of sample ...
-
bioRxiv - Immunology 2021Quote: ... The first-strand synthesis reaction was purified using the Ampure Size Select Magnetic Bead Kit at a ratio of 0.6X (Beckman Coulter). Following ...
-
bioRxiv - Genomics 2022Quote: Pieces of caudal fin sampled from 463 fish (288 from LB line and 175 from LC line) were sent to Gentyane genotyping platform (INRAE, Clermont-Ferrand, France) for DNA extraction using the DNAdvance kit from Beckman Coulter following manufacturer instructions and genotyping using the newly constructed HD SNP array.
-
bioRxiv - Molecular Biology 2024Quote: ... Libraries were prepared using 2S Plus DNA Library Kit adapters (Swift Biosciences) and size-selected using SPRIselect beads (Beckman Coulter) before being amplified and sent for paired-end sequencing on the NovaSeq 6000 (150 bp reads).
-
bioRxiv - Pathology 2024Quote: RNA from rat liver tissue was snap frozen in RNAlater and lysed using lysis buffer (RNAdvanced Kit, A32646, Beckman Coulter), 20µL Proteinase K on a TissueLyser II (Qiagen ...
-
bioRxiv - Genetics 2023Quote: ... clean-up and flowcell loading following the protocol for SQK-LSK112 sequencing kit (Version GDE_9141_v112_revC_01Dec2021) with the following modifications: Incubation with and elution from AMPure XP beads (Beckman Coulter, Cat. No. A63882) was extended to 30 minutes each ...
-
bioRxiv - Microbiology 2023Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2022Quote: ... The rest of the DNA purification process was performed using a GenFind v2 DNA extraction kit (Beckman Coulter, Indianapolis, U.S.) following the manufacturer’s protocol ...
-
bioRxiv - Evolutionary Biology 2023Quote: ... The upper aqueous phase was further processed for total RNA extraction using the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Germany) on a KingFisher Flex Purification System (Thermo Fisher Scientific ...
-
bioRxiv - Evolutionary Biology 2023Quote: We extracted DNA from fin clips collected from wild-caught fish using the Agencourt DNAdvance kit (Beckman Coulter, Brea, California). We used half-reactions but otherwise followed the manufacturer’s instructions for DNA extraction ...
-
bioRxiv - Microbiology 2023Quote: Subsequent second-strand synthesis was performed using the Sequenase 2.0 kit (Thermo #70775Z1000UN) and amplicons cleaned with 1.8 × AMPure XP magnetic beads (Beckman Coulter #A63882). Purified cDNA samples were stored at −20°C until library preparation.
-
bioRxiv - Developmental Biology 2019Quote: ... a purification step was performed using MiniELute Qiagen kit and size separation on Agencourt AMPure XP beads (A63882, Beckman Coulter, Inc) to remove PCR Nextera primers.
-
bioRxiv - Cancer Biology 2020Quote: ... Amplified libraries were purified with the Qiagen PCR clean-up MinElute kit and size selected for fragments less than 600 bp using the Agencourt AMPure XP beads (Beckman Coulter). Libraries were quality controlled by Qubit and Agilent DNA Bioanalyzer analysis ...
-
bioRxiv - Developmental Biology 2021Quote: ... cDNA was prepared using Takara Clontech SMART-Seq v4 Ultra Low Input RNA Kit for Sequencing (Cat.No.:634890) using 8 cycles of amplification and purified using Agencourt AMPure XP beads (Beckman Coulter, A63880). 1 ng of each sample cDNA was used for library preparation ...
-
bioRxiv - Microbiology 2020Quote: ... The resulting cDNA was PCR-amplified to about 10-20 ng/μl using a high-fidelity DNA polymerase followed by purification using the Agencourt AMPure XP kit (Beckman Coulter). The cDNA samples were pooled with ratios according to the RNA concentrations of the input samples and a size range of 200-550 bp was eluted from a preparative agarose gel ...
-
bioRxiv - Microbiology 2020Quote: ... The presence or absence of fecal occult blood in fresh pellets was determined using a Hemoccult blood testing kit (Beckman Coulter).
-
bioRxiv - Cell Biology 2021Quote: ... or Lin−Sca-1+c-Kit+CD150+CD48−CD34− (CD34−HSC) and Lin−Sca-1+c-Kit+ (LSK) cells were sorted on MoFlo Astrios EQ (Beckman Coulter). Quantification of CD71 ...
-
bioRxiv - Developmental Biology 2019Quote: ... with a final double (0.65 x −1 x bead volume) size selection step using a SPRIselect reagent kit (Beckman Coulter, B23318). The libraries were indexed (New England BioLabs ...
-
bioRxiv - Developmental Biology 2019Quote: Detection of apoptotic TCam-2 cells was performed 48 h after transfection of TCam-2 cells with control and KIF18A siRNA constructs using an Annexin V-FITC Apoptosis Detection Kit (Beckman Coulter), according to the manufacturer’s protocol ...
-
bioRxiv - Microbiology 2020Quote: ... The amplification products were checked using gel electrophoresis and were purified using the Agencourt AMPure XP Kit (Beckman Coulter, CA, USA). The purified products were the indexed in the 16S V4–V5 library ...
-
bioRxiv - Genetics 2021Quote: ... The fragment analysis was carried out using capillary electrophoresis in the Beckman Coulter CEQ8000 using the DNA Size Standard Kit-400 (Beckman Coulter) with 1.5 μl PCR product diluted in 20 μl formamide.