Labshake search
Citations for Beckman :
901 - 950 of 1189 citations for RT PCR kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Triplicate PCR reactions for each sample were then pooled and purified using Agencourt® AMPure® XP beads (#A63881, Beckman Coulter, UK).
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bioRxiv - Synthetic Biology 2023Quote: ... The PCR products were analyzed with 2% agarose gel and transferred into 96-well PCR plate wells for clean-up with 20 µL of AMPure XP beads (Beckman Coulter #A63881) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... The 230bp band was verified to be present and the amplified PCR products were cleaned up using double-sided SPRI via AMPure Beads (Beckman Coulter, A63880). Purified samples were normalized to a concentration of 10 nM using Qubit dsDNA HS Assay Kit (Invitrogen ...
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bioRxiv - Genomics 2023Quote: ... The tagmented DNA was amplified with eight cycles of PCR amplification and the libraries were cleaned up with SPRselect beads (Beckman Coulter, #B23317). The mean size of the library molecules and the quality of the libraries were determined on an Agilent Bio-analyser High Sensitivity DNA chip (Agilent technologies ...
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bioRxiv - Genetics 2024Quote: We separate the resulting product into long (“L”) and short (“S”) molecular libraries from the same PCR reactions by using SPRIselect beads (Beckman Coulter, #B23319). The next step involves further preparing libraries for long-read sequencing ...
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bioRxiv - Immunology 2021Quote: ... Env+ fraction and spike density were determined by single vesicle flow cytometry (113, 114), using a commercial kit (vFCTM Assay kit, Cellarcus Biosciences, La Jolla, CA) and flow cytometer (CytoFlexS, Beckman Coulter, Indianapolis, IN). Briefly ...
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bioRxiv - Pathology 2019Quote: ... PCR products were verified using gel electrophoresis (1% agarose gel) and cleaned with AMPure XP beads (Agencourt, Beckman Coulter Inc., Pasadena, CA, USA). Amplicons were then indexed using the Nextera XT Index Kit V2 set A and set D (Illumina ...
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bioRxiv - Neuroscience 2020Quote: ... Sequencing products were cleaned up using magnetic beads (CDTR-0050; CleanNA, Waddinxveen, The Netherlands) and according to the Agencourt AMpure PCR purification (Beckman Coulter, Brea, USA) protocol ...
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bioRxiv - Microbiology 2021Quote: ... Equimolar concentrations of PCR products were purified and pooled using a 0.8 volume of AMPure XP beads (Beckman Coulter Genomics, Danvers, MA, USA) and eluted with molecular water ...
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bioRxiv - Synthetic Biology 2022Quote: ... 20 μL reactions were prepared by dispensing 1 μL of each 10 μM reverse primer into the wells of a 96-well PCR plate using the Echo liquid handler (Beckman Coulter, Brea, CA). A mastermix consisting of polymerase premix ...
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bioRxiv - Molecular Biology 2019Quote: ... Non-target by-products were removed as required from some 12S PCRs by purification with magnetic Agencourt AMPure beads (Beckman Coulter, Krefeld -Germany).
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bioRxiv - Microbiology 2020Quote: ... The DNA from the 50 μL PCR reactions was purified using 60 μL Agencourt AMPure XP beads following manufacturers protocols (Beckman Coulter no. A63880). For the second PCR ...
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bioRxiv - Systems Biology 2019Quote: ... and divided into three 25-μL replicate reactions during both first- and second-step cycling reactions and cleaned after the first and second step using Agencourt AMPure XP-PCR purification (Beckman Coulter, Brea, CA) according to manufacturer instructions ...
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bioRxiv - Immunology 2021Quote: ... as well as the mRNA PCR product were separated and purified by double-sided size selection using AMPure XP magnetic beads (Cat. No. A63880; Beckman Coulter, Krefeld, Germany). A fraction of the Abseq/multiplex sample tag PCR 1 product ...
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bioRxiv - Genomics 2024Quote: ... Surplus PCR primers were further removed by purification using AMPure XP beads (SPRIselect beads for TT-seq and Frac-seq) (Beckman-Coulter, Villepinte, France) and the final cDNA libraries were checked for quality and quantified using capillary electrophoresis ...
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bioRxiv - Genomics 2024Quote: ... Illumina P5/P7 adapters were added during sample index PCR and the final libraries were cleaned using SPRIselect beads (Beckman Coulter cat. #B23318). For snRNA-seq ...
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bioRxiv - Neuroscience 2022Quote: ... pooled and purified with Agencourt AMPure XP kit (Beckman Coulter). Purified PCR products were quantified using a Qubit dsDNA HS assay kit (Life Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: ... and Agencourt® RNAClean® XP Kit (Beckman Coulter, USA). Measurement of RNA yield and quality was performed using Qubit® RNA HS assay kit (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: DNA was extracted using the GenFind v2 kit (Beckman Coulter) and libraries prepared using the NexteraFlex kit (Illumina Inc. ...
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bioRxiv - Microbiology 2022Quote: ... and the Agencourt RNAdvance Tissue Kit (Beckman Coulter, Krefeld, Germany). FFPE sections were loaded into a microTUBE-130 Screw-Cap (Covaris ...
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bioRxiv - Cancer Biology 2022Quote: ... single-stained beads (VersaComp Antibody Capture Bead Kit; Beckman Coulter) and a single-stained cell-mix of all samples analyzed were used ...
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bioRxiv - Bioengineering 2021Quote: ... were performed using the PerFix-nc Kit (Beckman Coulter #B31167), for 30 min at 4 °C in supplemented PBS containing 2 mM EDTA and 2% FBS ...
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bioRxiv - Microbiology 2021Quote: RNA was extracted using the RNAdvance Tissue kit (Beckman Coulter) and the KingFisher Flex System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... preamplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318). Paired V(D)J sequence were enriched with 10X primers ...
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bioRxiv - Microbiology 2023Quote: ... purified with the Agencourt AMPure XP kit (Beckman Coulter Genomics), and analyzed by capillary electrophoresis ...
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bioRxiv - Molecular Biology 2023Quote: ... and cleaned using the Agencourt RNAClean XP Kit (Beckman-Coulter) according to manufacturer’s instructions ...
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bioRxiv - Genetics 2023Quote: ... Samples were purified with the CleanSEQ kit (Beckman Coulter, A29151) following the Agencourt CleanSEQ Dye-Terminator Removal protocol ...
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bioRxiv - Molecular Biology 2021Quote: The VHH-coding regions within parent libraries and 1-round target-enriched sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Then ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Barcoded PCR products (234 bp) were pooled from triplicate tubes and purified by double-sided size selection using Ampure beads (Beckman Coulter, Pasadena, CA, USA). In brief ...
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bioRxiv - Genomics 2019Quote: ... We then conducted a PCR purification step aimed at removing primer dimer and unincorporated dNTPs using Agencourt AMPure XP beads (Beckman Coulter, Brea, CA, USA) at a 1:1 ratio (50 μL PCR product ...
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bioRxiv - Microbiology 2022Quote: ... and (III) the PCR products as the final construction of the libraries were purified with AMPure XP system (Beckman Coulter Inc., Indianapolis, IN, USA). Sequencing library size distribution quality control was performed with an Agilent 2100 Bioanalyzer (Agilent Technologies ...
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bioRxiv - Cancer Biology 2023Quote: The VHH-coding regions within the parent (mother) libraries and the target-panned sublibraries were PCR amplified and purified using AMPure XP beads (Beckman Coulter, High Wycombe, UK). Dual-indexed libraries were prepared and sequenced on an Illumina MiSeq37 (Illumina ...
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bioRxiv - Developmental Biology 2021Quote: ... and cleaned up with SPRIselect Reagent Kit (Beckman Coulter, Pasadena, CA).
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bioRxiv - Genomics 2019Quote: ... with cleanup in between steps using SPRIselect Reagent Kit (Beckman Coulter). The Post-ligation product was then amplified with a C1000 Touch Thermal Cycler during the Sample Index PCR ...
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bioRxiv - Microbiology 2020Quote: ... and purified with the AMPure XP Kit (Beckman Coulter GmbH, USA), quality checked using Agilent High Sensitivity DNA Kit (Agilent ...
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bioRxiv - Cell Biology 2021Quote: ... We used PerFix-nc Kit (Cat.No. B31167; Beckman Coulter GmbH, Germany) and Phalloidin-Atto-647 (Atto-Tec GmbH ...
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bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
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bioRxiv - Immunology 2022Quote: ... and Beta Mark TCR Vbeta Repertoire Kit (Beckman Coulter, cat. IM3497). The Vbeta Repertoire Kit contains 24 different anti-TRBV antibodies that come in 8 vials (A – H) ...
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bioRxiv - Immunology 2022Quote: ... After amplification and purification with SPRIselect Reagent Kit (Beckman Coulter, B23318) of RT products ...
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bioRxiv - Cell Biology 2021Quote: ... followed by 1,2X AMpure beads purification kit (Beckman Coulter, Brea, California) to keep only retro-transcribed single strand cDNA ...
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bioRxiv - Plant Biology 2021Quote: ... and then purified using an Agencourt AMPure Purification Kit (Beckman Coulter). Purified cDNA was quantified by a Qubit 3.0 Fluorometer (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... The enzymatic fragmentation and size selection (SPRIselect Reagent Kit, Beckman Coulter) were used to optimize the cDNA amplicon size followed by adding P5 and P7 sample indexes (used in Illumina sequencers) ...
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bioRxiv - Immunology 2019Quote: ... fixed and permeabilized using the PerFix EXPOSE kit (Beckman Coulter, #B26976) according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... with cleanup in-between steps using SPRIselect Reagent Kit (Beckman Coulter). Post-ligation product was amplified with a T1000 Thermal Cycler programed at 98 °C for 45 s ...
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bioRxiv - Immunology 2020Quote: ... pS6 staining was performed using a PerFix EXPOSE kit (Beckman Coulter). For evaluation of intracellular targets ...
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bioRxiv - Pathology 2019Quote: Intracellular staining was performed using the Intraprep™ kit (Beckman Coulter). Flow cytometry analysis were performed on a BD Pharmingen LSRFortessa® cytometer ...
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bioRxiv - Microbiology 2020Quote: ... DNA was then purified using AMPure XP beads kit (Beckman Coulter) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... gDNA was further purified using Agencourt AMPure XP Kit (Beckman Coulter). Then the purity ...
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bioRxiv - Plant Biology 2023Quote: ... purified using the Agencourt AMPure XP kit (Beckman Coulter Genomics, USA) and analyzed by capillary electrophoresis ...