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Citations for Beckman :
1101 - 1150 of 1698 citations for 7 TRIFLUOROMETHYL 2 3 4 5 TETRAHYDRO 1H BENZO B AZEPINE HYDROCHLORIDE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... This crude mitochondrial pellet was further purified on a discontinuous percoll gradient (buffer composition 15/23/40% sucrose) by centrifugation (40,000 x g, 1hr, 4°C) in an SW41 rotor (Beckman Coulter) and enriched mitochondria were collected from the 23-40% interface (adapted from [76]) ...
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bioRxiv - Cell Biology 2020Quote: ... Tubes were centrifuged twice at 100,000 x g for 70 min at 4°C with a Beckman ultracentrifuge (32Ti rotor from Beckman Coulter). Supernatants were removed and the pellets resuspended in phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2020Quote: ... Microbial cells were collected from to 2-3 L of water samples by centrifugation at 12,000 × g and 4°C for 20 min in a high-speed centrifuge (Beckman, USA). Total genomic DNA was extracted using AxyPrep™ Genomic DNA Miniprep Kit (Axygen ...
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bioRxiv - Microbiology 2020Quote: ... Two distinct fractions were collected from the first CsCl density gradient centrifugation (17,000 × g, 60 min, 4°C in a fixed angle MLA-55 rotor, Beckman Coulter), each were sedimented (4000 × g ...
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bioRxiv - Molecular Biology 2021Quote: ... lysates were spun for 24 h at 4°C at 50000 rpm in a Type 70 Ti rotor (Beckman Coulter) through a 40 % (w/v ...
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bioRxiv - Microbiology 2021Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free RPMI 1640 or DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Immunology 2020Quote: ... previously coated over-night at 4 °C with 1.5 μg per well of anti-Igκ or anti-IgM (Beckman Coulter) in PBS and saturated with culture medium ...
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bioRxiv - Biochemistry 2022Quote: ... A soluble fraction was obtained after centrifugation of the lysate at 19,000 rpm for 40 min at 4 °C performed using Avanti J-E with a rotor JA-20 (BECKMAN COULTER) to remove insoluble debris.
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... Ribosomes were pelleted by centrifugation at 63,000g for 16–19 hrs at 4°C using a Type 80 Ti rotor (Beckman Coulter). For all ultracentrifugation steps indicated here ...
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Dynamic states of eIF6 and SDS variants modulate interactions with uL14 of the 60S ribosomal subunitbioRxiv - Biochemistry 2022Quote: ... were pelleted by centrifugation at 63,000g for 20 hrs at 4°C in a Type 80 Ti rotor (Beckman Coulter) as described above ...
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bioRxiv - Cell Biology 2022Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with a 18G needle ...
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bioRxiv - Cell Biology 2022Quote: ... The lysate was dounced for 10 strokes on ice and cleared via centrifugation at 80K rpm for 10 minutes at 4°C using a TLA 110 rotor (Beckman) in a tabletop Beckm an ultracentrifuge ...
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bioRxiv - Plant Biology 2022Quote: ... in a 12.5 mL ultracentrifuge tube and centrifuged for 1 h at 4°C at 200,000 × g in a swinging bucket rotor (Sorval SW-41 Ti; Beckman Coulter), with moderate acceleration and deceleration ...
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bioRxiv - Microbiology 2022Quote: ... purified by ultracentrifugation through a sucrose cushion (25% weight/volume in Tris-NaCl-EDTA buffer) for 75 min at 4°C and 28,000 rpm using a SW 32 TI rotor (Beckman Coulter), resuspended in serum-free DMEM medium and stored in small aliquots at −80°C ...
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bioRxiv - Microbiology 2022Quote: ... Pseudotypes and VLPs were then concentrated by ultracentrifugation (20,000 RPM, 4 ºC, 1.5h, Beckman Coulter Optima XPN-100, SW32Ti rotor) through a 20% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... at 20 KPSI at 4°C and then centrifuged at 19 000 RPM in a JA-25.50 rotor (Beckman Coulter) for 30 minutes at 4°C ...
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bioRxiv - Neuroscience 2023Quote: ... followed by ultracentrifugation at 100,000 x g for 60 minutes at 4°C (SW 41 Ti Rotor, Beckman Coulter Inc). For size-exclusion chromatography (SEC ...
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SARS-CoV2 associated secretion of nanoLuciferase reports on virus and Virus-Like Particle productionbioRxiv - Molecular Biology 2022Quote: ... the samples were pelleted through a 20% sucrose in 120 mM HEPES cushion at 100,000xg for 2hrs at 4 °C with 45 Ti or 70 Ti fixed-angle rotor (Beckman-Coulter) depending on the volume of the sample ...
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bioRxiv - Molecular Biology 2022Quote: ... and the sucrose cushion was ultra-centrifuged for 1.5 hours at 80000 rpm and 4°C in TLA-100 rotor (Beckman Coulter). The pellet was resuspended in 100 µL of buffer F (50 mM HEPES-KOH pH 7.4 ...
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bioRxiv - Molecular Biology 2024Quote: ... the rest was loaded on top of sucrose gradient and ultracentrifuged (30000 rpm, 4°C, 18h, stop DECEL, slow, SW40/1Ti swinging bucket rotor, Optime L-90K, Beckman). 24 fractions were taken from top to bottom of the tube as 500 µL aliquots and precipitated by incubation with 50 µL of 0.15% sodium deoxycholate for 10 min at RT ...
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bioRxiv - Molecular Biology 2024Quote: ... aggregated proteins were removed by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was mixed with 62.7 g of solid ammonium sulfate and stirred overnight at 4 ºC ...
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bioRxiv - Molecular Biology 2024Quote: ... the mixture was spun at 13,894 rpm (16,000 rcf) at 4 ºC for 10 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The beads that accumulated at the bottom of the tube were resuspended in 200 µL of EM buffer A ...
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bioRxiv - Molecular Biology 2024Quote: ... they were collected by centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 20 min using the SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). Subsequently ...
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bioRxiv - Molecular Biology 2024Quote: ... and spun at 32,000 rpm (max 124,436 rcf) and 4 ºC for 13 h using an SW55Ti rotor in an Optima L80 centrifuge (Beckman Coulter). The samples were fractionated from the top of the sucrose gradient ...
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bioRxiv - Molecular Biology 2024Quote: ... Insoluble fractions were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fractions released by MNase were isolated by taking supernatants after centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 30 min using an SX241.5 rotor in an Allegron X-30R centrifuge (Beckman Coulter). The supernatants were collected and layered onto a 10-22 % linear sucrose gradient solution with buffer SG (15 mM HEPES-KOH [pH 7.4] ...
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bioRxiv - Molecular Biology 2024Quote: ... Insolubilized proteins were removed with centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The supernatant was loaded into the HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). Pellets containing fixed chromosomes were resuspended with 10 mL of buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... rpm at 4 ºC for 40 min using a JS 5.3 rotor in an Avanti J-26S centrifuge (Beckman Coulter). The chromosomes were collected from the bottom of the centrifuge tube and resuspended with buffer SC ...
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bioRxiv - Molecular Biology 2024Quote: ... The insoluble pellets were washed by resuspending them in 50 ml of buffer E and re-collecting them through centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). The washed insoluble pellets were resuspended in 8 mL of 6 M guanidine HCl (pH 1.5 ...
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bioRxiv - Molecular Biology 2024Quote: ... The denatured proteins were collected by centrifugation at 20,000 rpm at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). Protein concentrations in soluble fractions were estimated based on the absorbance at 260 nm ...
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bioRxiv - Genomics 2023Quote: ... 1x protease inhibitor cocktail EDTA-free) by spinning at 38,000 rpm at 4 °C in a SW-41 Ti rotor (Beckman Coulter). Monosome pellets were resuspended in 400 µL lysis buffer and rocked overnight ...
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bioRxiv - Biophysics 2024Quote: ... and subjected to ultracentrifugation at 384,492□g for 45□min at 4□°C (Beckman Coulter Optima MAX, TLA-100 rotor), stored at −80 °C after flash-frozen with liquid nitrogen for crystallization trials ...
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bioRxiv - Biochemistry 2024Quote: ... The protein samples were carefully applied to the gradient and centrifuged for 16 h at 130,000 g at 4 °C (Optima XPN-80 Ultracentrifugation from Beckman Coulter). 200 µl fractions were taken from the top of the gradient and the glutaraldehyde quenched with 20 mM Tris pH 7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The homogenate was clarified by centrifugation for 30 min at 70,560 × g at 4°C in an Optima XPN Ultracentrifuge (Beckman Coulter) using a Ti-45 rotor ...
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bioRxiv - Molecular Biology 2024Quote: ... Lysates were cleared by centrifugation for 1 h at 45,000 rpm at 4°C (Optima XPN-80 Ultracentrifuge with 70 Ti, Beckman Coulter). HTP-tagged proteins were immunoprecipitated with 600 µl pre-washed IgG-sepharose 6 fast flow affinity resin (GE Healthcare ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were centrifuged at 30,000 xg for 45 min at 4 °C using a SW41 Ti swinging bucket rotor (Beckman Coulter) before the supernatant was decanted ...
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bioRxiv - Neuroscience 2024Quote: ... The homogenate was centrifuged (45 min, 16 000 rpm, 4°C, JLA 16.250 rotor, Beckman-Coulter Centrifuge Avanti J25-01) and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was ultracentrifuged at 80,000 × g for 1 h at 4°C using a type 50 Ti rotor ultracentrifuge (Beckman Coulter). The supernatant ...
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bioRxiv - Cell Biology 2023Quote: ... the fifth virus was freeze-thawed three times and ultracentrifuged at 23 k rpm at 4 °C for 1.5 hours in the Super-centrifuge Optima XE-90 Ultracentrifuge (Beckman Coulter) using Ultra-Clear Centrifuge Tubes (Beckman Coulter) ...
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bioRxiv - Biochemistry 2023Quote: ... The gradient was centrifuged for 2.30 hours at 4 °C and 111,000 ×g in a swing-out rotor (Beckman- Coulter, SW41). The brownish lysosome-enriched fraction (∼ 1ml ...
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bioRxiv - Cell Biology 2023Quote: ... Small EVs were isolated by ultracentrifugation at 120,000 x g and 4°C for 120 min using Optima XE-90 equipment with a SW32Ti rotor (Beckman Coulter). Supernatants were discarded using a pipette ...
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Centriole and transition zone structures in photoreceptor cilia revealed by cryo-electron tomographybioRxiv - Cell Biology 2023Quote: ... OptiPrep step-gradient and centrifuged for 60 min at 19,210 × g at 4 °C in a TLS-55 rotor (Beckman Coulter). The ROS band was collected with an 18G needle ...
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bioRxiv - Systems Biology 2023Quote: ... Gradients were centrifuged at 4°C for 17 h at 100,000 g using an L7 ultracentrifuge with an SW28 swing out rotor (Beckman Coulter). Fractions (F ...
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bioRxiv - Microbiology 2022Quote: ... The eluted sample was collected by centrifugation at 2000 rpm for 1 minute 4 °C in a F241.5P rotor using a 149 Microfuge 22R centrifuge (Beckman Coulter). For SDS-PAGE analyses ...
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bioRxiv - Cell Biology 2023Quote: ... The iodixanol density gradients were centrifuged at 100,000 x g at 4◦C for 18 hours in SW41Ti rotor (Beckman Coulter). Ten fractions (F1 ...
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bioRxiv - Molecular Biology 2023Quote: ... and ultracentrifuged in a sucrose cushion at 100,000 rpm at 4°C for 1 h with a TLA110 rotor (Beckman Coulter). The ribosome pellet was dissolved in TRIzol reagent (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 25 000 rpm for 45 min at 4°C in a Ti45 rotor (Beckman). Supernatant was incubated with 5 ml of Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenised mixture then underwent ultracentrifugation at 200,000 g for 110 min at 4°C with a SW-41 Ti rotor (Beckman Coulter) and transferred the supernatant into a Slide-A-Lyzer™ G2 Dialysis Cassettes 2K MWCO (Thermo ...
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bioRxiv - Cancer Biology 2022Quote: ... Fractions 2–9 were diluted 1:10 in PBS and centrifuged at 20,000 ×g (bEVs) or 100,000 ×g (sEVs) at 4°C for 1 h with an MLS-55 rotor (Beckman Coulter). The bEV and sEV pellets were suspended in double 0.22-µm-filtered PBS and were either used directly for experiments or stored in aliquots at −80°C until subsequent BLI ...