Labshake search
Citations for Beckman :
1101 - 1150 of 2014 citations for 6 Fluoro 1 2 3 4 tetrahydro 2 methylquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Gradients were spun at 45,000 RPM for 16 hours at 4°C in SW55Ti rotor using an ultracentrifuge (Beckman). After centrifugation ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was then clarified (using a JA 25.50 Beckman rotor at 40000x g for 30 min, 4°C) and the soluble fraction was incubated with 2 ml of Glutathione Sepharose 4B resin for 2h at 4°C under constant agitation ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 μg/mL cycloheximide if reaction contained cycloheximide) for 3.5 hrs at 240,000 g at 4 °C using a SW41 rotor (Beckman Coulter). Gradients were fractionated using Teledyne Isco Tris Peristaltic Pump and fractions were collected and pooled according to the UV trace ...
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bioRxiv - Biochemistry 2023Quote: ... Density gradient centrifugation was done at 50,000 rpm for two hours at 4 °C using a 70.1 Ti rotor (Beckman Coulter). Post centrifugation ...
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bioRxiv - Immunology 2023Quote: ... and then centrifuged at 260,800 x g for 2.5 h at 4°C in an Optima L-100XP ultracentrifuge (Beckman–Coulter) using the SW41Ti rotor with brake ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and Red-C12 fluorescence of the starting material ...
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bioRxiv - Genomics 2023Quote: ... followed by centrifugation at 31,900 ×g (10 min, 4 °C, JA-20 rotor, Avanti J-26 XP, Beckman Coulter). Next ...
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bioRxiv - Biophysics 2023Quote: ... and the resulting lysate was cleared by centrifugation at 279,288 × g for 10 minutes at 4°C using a tabletop centrifuge unit from Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... After cell debris and undisrupted cells were removed by centrifugation (45,000 rpm, 30 min, 4°C, 70Ti rotor; Beckman), supernatant was applied to an open column with Ni-IMAC resin (BIO-RAD) ...
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bioRxiv - Biochemistry 2023Quote: ... at 18000 PSI and cell debris were separated by centrifugation (4 °C, 30 min, 75,600 ×g, JA25.50 rotor Beckman Coulter). The supernatant was loaded on an affinity chromatography column (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Equilibrated tubes were ultra-centrifuged at 100,000xg for 1h at 4°C using a swinging-bucket rotor (SW 32Ti, Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... of proteins were applied on the top of a 16 ml 10-30% continuous sucrose density gradient and centrifuged at 74,400 g for 15 h at 4 °C (SW 32.1 Ti; Beckman Coulter). 24 equal volume fractions were collected ...
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bioRxiv - Molecular Biology 2023Quote: ... Small extracellular vesicles were pelleted at 100,000 X g for 1,5 h at 4°C by using Ti-45 rotor (Beckman Coulter) and 70 ml polycarbonate bottles (Beckman Coulter ...
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bioRxiv - Molecular Biology 2023Quote: ... Pellets were pooled in 1 ml PBS and centrifuged at 100,000 X g for 1,5 h at 4°C in 1,5 ml tubes in a Ti-45 rotor (Beckman Coulter) using adapters (#11004 ...
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bioRxiv - Plant Biology 2023Quote: ... The cel lysates were clarified by high-speed centrifugation at 50,000g for 60 min at 4°C (Avanti J-26 XP centrifuge with JA-25.50 rotor; Beckman Coulter), followed by a 0.45-mm filtration (Millex-HP ...
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An adaptive biomolecular condensation response is conserved across environmentally divergent speciesbioRxiv - Cell Biology 2023Quote: ... 500 µL was transferred to a vacuum safe tube spun at 100,000 g for 20 minutes at 4°C (fixed-angle TLA-55 rotor in a Beckman Coulter Optimax tabletop ultracentrifuge) ...
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bioRxiv - Systems Biology 2023Quote: ... Two libraries were prepared and sequenced: one with size-based selection for mRNA >4 kb using AMPure beads (Beckman) and a second library with no size selection.
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bioRxiv - Microbiology 2023Quote: ... The denatured protein fraction was then removed via ultracentrifugation at 100,000 g for 20 min at 4°C (Beckman Coulter Optima XE-90 – IVD ultracentrifuge with a 42.2 Ti rotor) ...
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bioRxiv - Cell Biology 2024Quote: ... Solutions of 15% and 23% Optiprep were layered on top or below the sample and centrifuged at 4°C for 16 h at 120,000g in a SW 28 Ti swinging bucket rotor (Beckman).
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bioRxiv - Cell Biology 2024Quote: ... 0.1 mM PMSF buffer and subjected to ultracentrifugation at 36,000 rpm at 4°C for 2h using a SW41 rotor (Beckman) and 14 x 89 mm polypropylene tubes (Beckman ...
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bioRxiv - Microbiology 2024Quote: ... Samples within each biological replicate were centrifuged simultaneously for 2.5 h at 209,490 x g and 4°C using the SW41Ti rotor in an LE-80 ultracentrifuge (Beckman). Subsequently ...
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bioRxiv - Biophysics 2024Quote: ... Lysates were clarified by ultracentrifugation at 34,000 rpm for 45 minutes at 4°C in a Type 45.1 Ti rotor (Beckman-Coulter). The supernatants were then passed through a 0.22 μm syringe sterile filter (Thermo-Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... and the membranes were pelleted at 100,000 g for one hour at 4°C in a TLA 100.3 rotor (Beckman-Coulter). Afterwards the supernatant was taken off and stored at −20°C (“S100” ...
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bioRxiv - Biochemistry 2024Quote: ... The unbroken cells and debris were removed by centrifugation at 20,000 x g for 10 min at 4 °C (Beckman Coulter Avanti J20 XPI centrifuge ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 minutes, 4 °C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of α-FLAG M2 affinity gel (Sigma–Aldrich ...
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bioRxiv - Cell Biology 2024Quote: ... The lysate was clarified by centrifugation (66,000 x g, 30 minutes, 4 °C) in a Type 70Ti rotor (Beckman). For His-ZZ-TEV-Halo-CCSer2650–850 ...
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bioRxiv - Biochemistry 2024Quote: ... dimethylammonio)-1-propanesulfonate)] and lysates were cleared by centrifugation at 75,600 g at 4 °C for 30 min using a JA 25.50 rotor (Beckman-Coulter). Next ...
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bioRxiv - Neuroscience 2024Quote: ... EV-depleted FBS medium was obtained by the ultracentrifugation of BV-2 complete medium supplemented with 20% FBS for 18h at 100.000 × g and 4°C in 38.5 mL polyallomer Ultra Clear ultracentrifuge tubes (Beckman Coulter), using an Optima XPN-80 ultracentrifuge and an SW 32 Ti Rotor (Beckman Coulter ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were ultracentrifuged at 100,000 × g for 135 min at 4°C (MLA-55 rotor, Beckman Coulter, USA). After removal of the supernatant ...
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bioRxiv - Cell Biology 2024Quote: ... The gradients were centrifuged at 39,000 rpm for 2.5 h at 4°C using a SW41Ti rotor (Beckman Instruments), and 21 fractions of 0.5 ml were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... MgCl2 5mM and Tris-Cl 50mM pH 7.4) was added into 3-mL polycarbonate tube for an ultracentrifuge TL100.3 rotor (Beckman Coulter, Brea, CA, USA). The KCl-adjusted ribosome-containing solution was carefully added above the sucrose cushion and tubes were balanced by weight using buffer B (sucrose 250mM ...
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bioRxiv - Molecular Biology 2021Quote: ... Produced amplicons of 3-144 µm eukaryotes and Imitervirales were purified with the magnetic beads (Agencourt AMPure XP beads, Beckman Coulter, Inc.). Finial DNA production was dissolved in 27.5 µL ultrapure water ...
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bioRxiv - Cell Biology 2021Quote: ... supplemented with 100 μg/ml cycloheximide) and centrifuged for 3 hours at 35,000 rpm in an SW41 rotor in a Beckman L7 ultracentrifuge (Beckman Coulter, Krefeld, Germany). The gradients were simultaneously fractionated on a Density Gradient Fractionation System (#621140007 ...
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bioRxiv - Biochemistry 2022Quote: ... Average fluorescence intensity of DCF from 3×105 cells was recorded and data acquisition and analysis were carried out using Summit 5.2 software (Beckman-Coulter, Inc. USA). Morphology of algal cells was examined and photographed with a differential interference contrast microscopy (DIC ...
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bioRxiv - Cell Biology 2022Quote: ... The gradient was formed by sequentially adding each 3 ml layer to a 14 × 89 mm ultra-clear centrifuge tube (Beckman Coulter, Inc.), starting with the 40% solution and ending with the 5% solution ...
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bioRxiv - Zoology 2021Quote: ... Food concentration was maintained at 1500 μm3 mL−1 of phytoplankton cells at the outlet of the tank – ad libitum (Rico-Villa et al., 2009) – and controlled twice daily using an electronic particle counter (Multisizer 3, Beckman Coulter, USA) equipped with a 100 μm aperture tube ...
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bioRxiv - Neuroscience 2022Quote: ... and a 200-μl aliquot of the final supernatant was added to 3 mL of scintillation fluid and the radioactivity was measured by a liquid scintillation counter (Beckman LS 6000SC). Blank values were obtained by performing the reaction in the absence of tissue homogenate and in the presence of boiled homogenate ...
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bioRxiv - Biochemistry 2024Quote: 3 L of over-expressed cells were harvested immediately by centrifugation (Beckman Coulter Avanti J-20 XPI centrifuge, Beckman JLA-8.100 rotor) at 6,000 x g for 10 min at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... conditioned media were subjected to 10 min at 200g for 3 cycles (G146D centrifuge, Gelec®) and 20 min at 2000g (Allegra 64R Centrifuge, Beckman Coulter) to remove cellular debris and apoptotic bodies respectively ...
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bioRxiv - Biochemistry 2021Quote: ... 10 U/mL SuperASE-In and was ultracentrifuged at 100,000 × g for for 16 h at 4 °C in a SW Ti41 rotor (Beckman Coulter). After removing the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... The lysate was clarified by ultracentrifugation at 30,000 × g 30 min at 4°C using a Ti45 rotor (Beckman Coulter) twice ...
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bioRxiv - Biophysics 2020Quote: ... Escherichia coli BL21 cells overexpressing α-synuclein were collected by centrifugation (20 min, 4000 rpm, 4 °C) in a JLA-8.1000 rotor in a Beckman Avanti J25 centrifuge (Beckman Coulter), resuspended in lysis buffer (10 mM Tris ...
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bioRxiv - Biophysics 2020Quote: ... The insect cells were harvested after 4 days by centrifugation at 300 g for 10 min at 4 °C in an Avanti J-26S ultracentrifuge (JLA-9.1000 rotor, Beckman Coulter). The cell pellet was resuspended in 5 ml ice-cold phosphate buffered saline (PBS ...
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bioRxiv - Biophysics 2020Quote: ... and centrifuged at 45000 x g for 60 min at 4 °C in the Avanti J-26S ultracentrifuge (JA-30.50Ti rotor, Beckman Coulter). The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... and the gradient was centrifuged for 60 min at 24,700 × g at 4 °C using a TLS-55 swinging bucket rotor (Beckman Coulter). Purified mouse POS were resuspended in DMEM/Ham’s F-12 containing 2.5% sucrose ...
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bioRxiv - Cell Biology 2020Quote: ... lysed by sonication (UD-201 ultrasonic disrupter, Tomy Seiko), and centrifuged at 50,000 rpm (70 min, 4°C) with a 70 Ti rotor (Beckman Coulter). GST-His6-tagged LC3B-His12 and GATE-16-His12 proteins in the supernatants obtained were isolated by mixing with COSMOGEL GST-Accept beads (50% slurry ...
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bioRxiv - Immunology 2021Quote: ... the culture medium containing the lentiviral particles was collected and ultracentrifuged at 25,000 rpm for 90 min at 4 °C using a Beckman SW28 ultracentrifuge rotor (Beckman Coulter) for the obtention of concentrated virus ...
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 27,000 x rpm for 16 hrs at 4°C in an Optima L-100 XP ultracentrifuge (Beckman Coulter) using a SW32Ti rotor.
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bioRxiv - Microbiology 2020Quote: ... sanguinis was grown as described above and centrifuged for 10 minutes at 3,740 x g in an Allegra X-142 centrifuge at 4°C (Beckman-Coulter). The supernatant was discarded and the cells were resuspended in BHI prior to inoculation ...