Labshake search
Citations for Beckman :
1051 - 1100 of 2014 citations for 6 Fluoro 1 2 3 4 tetrahydro 2 methylquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... Membranes were collected by ultracentrifugation at 220 000 x g for 90 min at 4°C (45Ti rotor, Beckman).
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bioRxiv - Biochemistry 2021Quote: ... and the lysate was clarified by centrifugation at 30,000 x g for 60 min at 4 °C in a JA25.50 rotor in an Avanti J-E centrifuge (Beckman). The clarified lysate was then added to 10 mL of Ni-NTA Agarose beads (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL glutathione-sepharose (Fisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... The lysate was clarified by centrifugation for 30 min at 35,000 rpm at 4°C in a Type 45 Ti rotor (Beckman). Cleared lysate was incubated with 2 mL Ni-NTA agarose (Qiagen ...
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bioRxiv - Immunology 2022Quote: ... stained bacteria were then sonicated for 4 min (0.5 cycle, 75 A, VialTweeter) followed by determination of concentration using flow cytometry (CytoFlex; Beckman Coulter ...
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bioRxiv - Microbiology 2022Quote: ... Fractions were diluted in 5 ml PBS and washed by ultracentrifugation at 200,000 × g (60 min, 4°C, AH-650 rotor, 5 ml Beckman Ultra-Clear tubes ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor ...
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bioRxiv - Microbiology 2022Quote: ... 100 ml cell cultures were grown over night and harvested by centrifugation at 15,000 x g for 15 min at 4 °C (Beckman JLA-16.250 rotor, Beckman Avanti J-25, Beckman Coulter). Pellets were washed with 20 mM potassium phosphate (pH 7.5 ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant fractions were collected and centrifuged at 75,600 x g for 90 min at 4 °C (Beckman JA25.50 rotor, Beckman Avanati J-25, Beckman Coulter). The resulting pellets were washed again and resuspended in 500 μl potassium phosphate buffer ...
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bioRxiv - Biochemistry 2022Quote: ... The lysate was clarified by centrifugation at 48000×g for 45 min at 4°C using a JA-25.50 rotor (Beckman), and applied on a XK 16/20 chromatography column (Cytiva ...
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bioRxiv - Biochemistry 2022Quote: ... The elutions were combined and centrifuged at 100,000 rpm at 4°C for 40 minutes in a TLA120.2 rotor (Beckman Coulter) before resuspension of the ribosomal pellet in RNC buffer containing 1 μM ternatin-4 ...
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bioRxiv - Developmental Biology 2022Quote: ... Gradients were centrifuged at 35.000 g for 3h at 4°C in an ultracentrifuge using the SW40ti rotor head (Beckman). Fractions of 1 mL were collected from the top at 1 mL/min ...
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Reducing mitochondrial ribosomal gene expression does not alter metabolic health or lifespan in micebioRxiv - Cell Biology 2022Quote: ... and then purified using 30% Percoll gradient ultracentrifugation at 95,000g for 30 minutes at +4°C (Beckman sv41 rotor). Purified mitochondria were treated 1:200 with a protease inhibitor cocktail (Thermo Fisher Scientific HALT PI ...
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bioRxiv - Genomics 2022Quote: ... All samples were acquired on a Beckman Coulter CytoFLEX S with 4 lasers (405nm, 488nm, 561nm, 638nm) (Beckman Coulter), and operated by CytExpert Software v2.3 (Beckman Coulter ...
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bioRxiv - Neuroscience 2022Quote: ... and ultracentrifuged at 25,000 g for 20 min 4°C (or 50,000 g for 30 min 4°C) in a Beckman TLA100.3 rotor (Optima™ TLX Ultracentrifuge, Beckman). The pellet was discarded ...
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bioRxiv - Microbiology 2022Quote: ... The gradient was centrifuged at 4°C and 35,000 rpm for 2.5 h using an SW 40 Ti rotor (Beckman Coulter). Afterward ...
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bioRxiv - Microbiology 2022Quote: ... which was spun in a ultracentrifuge at 30,000 x g for 2h at 4°C (SW 60 Ti rotor, Beckman) The pellet was resuspended in 20 μl of PBS for 1 h at 4 °C ...
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bioRxiv - Microbiology 2022Quote: ... Glycoproteins in the supernatants were pelleted by centrifugation at 100,000×g at 4°C for 90 min using a TLA-55 rotor (Beckman). The resultant pellets ...
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Ribonuclease Inhibitor and Angiogenin collaboratively regulate cell-type-specific global translationbioRxiv - Biochemistry 2024Quote: ... and were centrifuged at 39,000 rpm for 2.45 hours at 4°C using Optima XPN-80 Ultracentrifuge (Beckman Coulter). Following centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... Virus was then purified by centrifugation through a 20% sucrose cushion at 25,000 rpm for 2.5 hours at 4 °C in a Sw32Ti rotor (Beckman Coulter). Virus pellets were resuspended in PBS and virus genomic RNA copy number was quantified by RTqPCR as described above ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fraction was collected by centrifugation at 20,000 rpm (46,502 rcf) at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) and applied to a HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... and the soluble fraction was collected by centrifugation at 20,000 rpm (46,502 rcf) at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter). This fraction was then mixed with Ni-NTA agarose beads (Qiagen) ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fraction was collected with centrifugation at 20,000 rpm (46,502 rcf) at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) and applied to the HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... the beads were collected with centrifugation at 13,894 rpm (16,000 rcf) at 4 ºC for 20 min using SX241.5 rotor in Allegron X-30R (Beckman Coulter) and resuspended with 200 µL of PBS containing 15∼30 % glycerol ...
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bioRxiv - Molecular Biology 2024Quote: ... The soluble fraction was collected through centrifugation at 20,000 rpm (46,502 rcf) at 4 ºC for 30 min using a 45Ti rotor in Optima L80 (Beckman Coulter) and applied to the HisTrap HP column (Cytiva) ...
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bioRxiv - Molecular Biology 2024Quote: ... and spun at 32,000 rpm (max 124,436 rcf) and 4 ºC for 13 h using SW55Ti rotor in Optima L80 (Beckman Coulter). The centrifuged samples were fractionated from the top of the sucrose gradient ...
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bioRxiv - Neuroscience 2024Quote: ... by centrifugation at 5,000 x g at 4°C for 30 minutes in a fixed angle rotor (Beckman Coulter). The qEV column washed with 1x PBS was then loaded with 500 µL of concentrated supernatant followed by 2.5 mL PBS to collect 3 mL void volume ...
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bioRxiv - Neuroscience 2024Quote: ... and PBS was added to reach a 4.5 mL tube filling and centrifuged at 120,000xg for 100 min at 4°C in the L-60 Optima Ultracentrifuge (Beckman Coulter ...
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bioRxiv - Biophysics 2024Quote: ... Each seed mix was clarified using an ultra-centrifuge (Beckman Optima MAX-XP, 90,000 RPM, 4°C, 5 min) then snap-frozen in small aliquots and stored at -80°C ...
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bioRxiv - Biochemistry 2023Quote: ... lysates were centrifuged at 60,000 x g (4 °C) for 45 min (Beckman Avanti JXN-26 (rotor: JA 25.5)) and immediately supplemented with 10 mL pre-washed Ni2+-NTA agarose (QIAGEN ...
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bioRxiv - Neuroscience 2023Quote: ... DTT-treated EVs were pelleted at 100,000 x g for 70 min at 4°C in a TLA100 rotor (Beckman). The supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The prepared gradients were centrifuged at 200,000 x g for 16 h at 4°C in an SW41Ti rotor (Beckman), with no braking during deceleration ...
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bioRxiv - Neuroscience 2023Quote: ... The pellet was resuspended in 4.5 volumes of ice-cold extraction buffer and re- centrifuged at 11,300 RPM for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and combined with the first supernatant ...
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bioRxiv - Neuroscience 2023Quote: ... The tube was topped up to 23 mL with PBS and centrifuged at 59,300 RPM for 30 min at 4°C using a Ti70 rotor (Beckman). The floating lipid layer and supernatant were discarded and the pellet was transferred to a 2 mL tube in 50 μL PBS per g initial grey matter ...
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bioRxiv - Neuroscience 2023Quote: ... The homogenate was centrifuged at 11,300 revolutions per minute (RPM) for 10 min at 4°C using a Ti45 rotor (Beckman). The supernatant was filtered through a 70 μm pore size cell-strainer and retained on ice ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatant was transferred to fresh tubes and centrifuged at 100,000 x g for 70 min at 4°C using a Ti45 rotor (Beckman). The 100,000 x g pellets were resuspended in 1 mL ice-cold PBS and brought to 60 mL using PBS ...
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bioRxiv - Microbiology 2022Quote: ... Cell debris was removed by centrifugation at 14,800 rpm for 20 minutes 4 °C in F241.5P rotor using 149 Microfuge 22R centrifuge (Beckman Coulter). The supernatant was combined with 100 µL of ANTI-FLAG M2 Affinity Gel (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... The lysate was centrifuged at 40,000 rpm for 50 min at 4°C (45 Ti fixed-angle rotor, Beckman) and the transporter-Fab complex was isolated by affinity chromatography using Strep-Tactin resin ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were lysed by extrusion using a Teflon Dounce homogenizer and clarified by centrifugation at 30000g for 40 minutes at 4°C using a Ja 25.50 rotor (Beckman). The clarified lysate was incubated with 2 ml of pre-equilibrated Protein C affinity resin (Roche ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were ultracentrifuged at 100,000 g for 30 minutes at 4°C in a Optima XPN-100 ultracentrifuge (Beckman). Supernatants were kept aside as “Supernatant” fraction ...
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bioRxiv - Microbiology 2023Quote: ... All centrifugation steps were performed at 4 °C in an Avanti J-E high-speed fixed-angle centrifuge (Beckman Coulter Life Sciences ...
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bioRxiv - Plant Biology 2023Quote: ... Approximately 250 µl of crude EV sample resuspended in 20 mM Tris-HCl pH 6.0 was slowly dispensed on top of the column and centrifuged at 100,000 x g for 17 h at 4 ⁰C (SW 41 Ti rotor, Beckman Coulter ...
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bioRxiv - Cell Biology 2023Quote: ... 20µM ATP and 1 mM DTT and subsequently sedimented (100,000 x g, 15 min, 4°C, TLA-100 rotor) in an Ultima MAX-XP ultracentrifuge (Beckman). For competition assays ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged (33.500 rpm/4°C/3h, brake off) in a Optima XPN-80 ultracentrifuge (Beckman Coulter, Krefeld, Germany). Sucrose gradients were eluted into a BR-188 density gradient fractionation system (Brandel Inc ...
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bioRxiv - Biophysics 2023Quote: ... placed in an An50Ti rotor and centrifugated 50,000 rpm at 4 °C using an Optima XL-I analytical ultracentrifuge (Beckman). The data were analysed in SEDFIT-16.1c 53 using a c(s ...
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bioRxiv - Biochemistry 2023Quote: ... at 40% on a Branson Digital Sonifier and clarified by centrifugation for 30 minutes at 27,216 × g at 4°C in an SS-34 rotor (Avanti J-25 centrifuge, Beckman).
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation (183,960 x g, 88 min, 4°C) in a Type 70Ti rotor (Beckman). The supernatant was mixed with 2 mL of IgG Sepharose 6 Fast Flow beads (Cytiva ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified via centrifugation (66,000 x g, 30 min, 4°C) in a Type 70 Ti rotor (Beckman). The supernatant was mixed with 1.5 mL of anti-FLAG M2 affinity gel (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... then samples were centrifuged at 2,700 RPM for 10 minutes at 4°C in a tabletop centrifuge (Beckman Coulter). The supernatant (serum ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was centrifuged at 200,000 x g for 2.5 h (high g) at 4°C in a TLA-100.3 fixed angle rotor (k-factor 14) (Beckman Coulter) and the pellet was resuspended in 25 ∝l lysis buffer ...