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Citations for Beckman :
1101 - 1150 of 1791 citations for 4 1 tert butoxycarbonyl piperidin 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... and ultracentrifuged at 200,000 × g for 4 hours using a SW 55 Ti rotor in a Beckman OPTIMA90X ultracentrifuge (Beckman Coulter, USA). Finally ...
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bioRxiv - Biophysics 2023Quote: ... the SWCNT suspension was centrifuged at 30000 rpm (164 000 x g) for 4 h at 25 °C (Optima XPN-80, Beckman Coulter) to remove any remaining nanotube aggregates.
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bioRxiv - Genomics 2023Quote: ... cellular/protein debris were then collected by centrifugation at 1,000xg for 15 minutes at 4°C (Beckman Coulter Allegra X-14R), and the supernatant (DNA ...
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bioRxiv - Biophysics 2022Quote: ... EVs were separated from protein aggregates and free reagents by bottom-up density gradient centrifugation in sucrose for 17.30 h at 192,000 g and 4°C using a SW40 rotor (k-factor 144.5; Beckman Coulter, Fullerton, California, USA). Twelve fractions of 1 mL were then collected from the top of the gradient and respective densities were determined by refractometry using an Atago Illuminator (Japan) ...
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bioRxiv - Cancer Biology 2023Quote: ... and centrifuged at 100,000 x g either for 4 hr (hollow fiber bioreactor) or overnight (2D flask culture) in a SW32 rotor (Beckman Coulter). For the preparation of the density gradient ...
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bioRxiv - Plant Biology 2023Quote: ... The cAW was collected by spinning at 700 x g for 20 min at 4 ⁰C (JA-14 rotor, Beckman Coulter; Avanti J-20TM centrifuge, Beckman Coulter). vAW was filtered through a single layer of Miracloth and was combined with the cAW ...
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bioRxiv - Microbiology 2023Quote: ... The normalized cultures were then centrifuged at 5030 x g and 4°C for 20 minutes on an Avanti J-26 XPI (Beckman-Coulter) centrifuge (JS 5.3 rotor) ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were balanced and subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tubes were subjected to centrifugation at 135,000 × g for 2 h at 4 °C using a fixed-angle rotor (TLA-55, Beckman Coulter, USA). After DGUC ...
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bioRxiv - Biochemistry 2023Quote: ... Mitochondrial crude was pelleted by centrifugation at 9500 ×g (4 °C, 15 min) using the Optima XE-90 Ultracentrifuge with a Ti-45 rotor (Beckman Coulter). The final pellet was resuspended in 2 mL of Resuspension buffer (250 mM sucrose ...
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bioRxiv - Biochemistry 2023Quote: ... then centrifuged at 27,000 gmax for 15 min at 4°C (Optima XPN-100 Ultracentrifuge, Beckman Coulter, with SW60 Ti rotor). Resuspension of the pellet (sub-mitochondrial particles ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were resuspended in 100µL of PBS and stored at 4°C in the dark until samples were analyzed on a CytoFLEX LX Flow Cytometer (Beckman Coulter). Data was analyzed using FlowJo® software.
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bioRxiv - Cell Biology 2024Quote: ... to a volume of ∼12 mL with PBS and separated by ultracentrifugation at 160.000 × g for 2 h (4 °C) in a SW Ti41 rotor (Beckman Coulter #331362). Top-layer LDs were collected by aspiration using a pre-wetted 200 µL pipette tip so that maximal LDs were collected in minimal buffer volume ...
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bioRxiv - Bioengineering 2024Quote: ... we first measured a cell line population in PBS suspension using a coulter counter (Beckman Coulter Multisizer 4, 100 μm aperture) to obtain a cell volume distribution with the standard unit ...
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bioRxiv - Cell Biology 2024Quote: ... We loaded 2 mL of the homogenate mixture on top of a 3.92 ml 62% sucrose in MAB cushion in an Ultra-Clear 5/8” X 4” Beckman Coulter tube (344061, Beckman Coulter). We added the remaining sucrose in MAB solutions ...
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bioRxiv - Microbiology 2024Quote: ... Samples were balanced and centrifuged at 42,000 rpm for 35m at 4°C in a SW55Ti rotor and buckets with tube adaptors (Beckman REF: 356860). The supernatant was then concentrated to 80μl by spinning through Amicon 3kDa MWCO concentrator units at 14,000xg for 15-30m at 4°C ...
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bioRxiv - Plant Biology 2024Quote: ... The tubes were then centrifuged for 20 min at 700 x g with slow acceleration at 4°C using a JA-14 rotor (Avanti J-20 XP centrifuge; Beckman Coulter). The resulting apoplastic wash collected was passed through a 0.22 µm membrane filter to remove bacteria and larger debris ...
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bioRxiv - Microbiology 2024Quote: Bacterial isolates were grown overnight on LB agar and DNA extraction was performed using the GenFind v3 gDNA extraction kit according to standard protocol (Supp. Table 4) (Beckman Coulter). For the mock microbial community ...
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bioRxiv - Microbiology 2024Quote: ... This sample was then ultracentrifuged at 150,000 g for 2.5 h at 4 °C using a Type 70 Ti or Type 45 Ti rotor in an Optima XE Ultracentrifuge (Beckman Coulter). The translucent VLP pellet formed by this process was resuspended in 200 μl PBS buffer ...
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bioRxiv - Microbiology 2024Quote: ... Particles from the filtrate were concentrated by ultracentrifugation at 100,000 x g for 2 h at 4°C using an SW32Ti rotor in an Optima LE-80K centrifuge (Beckman Coulter). The pellets from different centrifuge tubes were pooled and resuspended in 1 mL of 1X PBS and pelleted again by ultracentrifugation at 165,700 x g for 2 h at 4°C in a TLA-55 rotor (Beckman Coulter) ...
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bioRxiv - Immunology 2024Quote: ... Centrifugation was performed (30 min, 800 g, 4 °C) in a swinging bucket on a Beckman Coulter JS-13.1 rotor (Beckman Coulter, USA). Hemocytes concentrated at each density interface were collected separately with a 70 mm long 20Gx2.75” needle mounted on a 1 mL syringe ...
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bioRxiv - Molecular Biology 2024Quote: ... The obtained culture was pelleted at low speed (7000 rpm for 10 minutes at 4 0C in a Beckman Coulter centrifuge). Harvested cells were resuspended (1 g of cell pellet per 10 mL ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... The total number of microglia (CD11b+ CD45+ cells) in the L3/4 spinal cord or SDH was analyzed using CytoFLEX SRT (Beckman Coulter) and FlowJo software (TreeStar) ...
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bioRxiv - Molecular Biology 2024Quote: ... lysed by sonication and ultracentrifuged at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). The clarified lysate was loaded onto pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was then subjected to ultracentrifugation at 26K RPM at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell-free extract so prepared was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The supernatant was subjected to ultracentrifugation at 26K rpm at 4 °C for 2 h (using optima XPNs Ultra Centrifuge, Beckman Coulter). Cell lysate was loaded onto 1 ml pre-equilibrated Ni-NTA column ...
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bioRxiv - Molecular Biology 2024Quote: ... The tube was centrifuged at 38,000 x g for 21-24 h at 4°C using the SW40T1 rotor in the Optima L-60+ Ultracentrifuge (Beckman Coulter). After ultracentrifugation ...
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bioRxiv - Neuroscience 2024Quote: ... The combined supernatants were centrifuged at 100,000 g for 40 min (4°C) on a TL110 rotor (Beckman Coulter, Paris, France) and the pellet was suspended in the same buffer ...
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bioRxiv - Biophysics 2024Quote: ... SV experiments were performed at 20°C by UV intensity detection using a 4-hole An-60 Ti rotor and standard 2-channel 12-mm Epon charcoal-filled centerpieces (Beckman Coulter). Samples were run at 26,000 rpm ...
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bioRxiv - Genomics 2024Quote: ... We then pelleted this concentrate by centrifugation in a swinging bucket centrifuge (25,000g, 2 hours, 4°, Beckman Coulter SW 32 Ti) and resuspended it in cold PBS + 4% glucose ...
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bioRxiv - Biochemistry 2024Quote: ... After centrifugation at 60,000 x g for 20 min at 4°C in a swing-out rotor (MLS-50, Optima Max-XP, Beckman Coulter), the mitochondrial fraction localized in the interphase between the two sucrose layers was carefully removed with a syringe ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were centrifuged at 4°C for 24 h at 274,400 x g in a SW-40Ti rotor (Beckman Coulter Inc.). To remove KBr ...
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bioRxiv - Cancer Biology 2024Quote: ... supernatants were ultracentrifuged at 120,000 to 140,000 x g (SW-28 rotor) for 2 hours at 4°C to pellet EVs on an OptimaTM L-100 XP ultracentrifuge (Beckman Coulter). The supernatant was removed ...
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bioRxiv - Cell Biology 2024Quote: ... cells were resuspended in PBS and stored in the dark at 4°C until analyzing them with a Navios flow cytometer (Beckman Coulter). At least 10’000 living cells were measured for each sample ...
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bioRxiv - Biochemistry 2023Quote: ... Colorimetric changes was measured 3 min after mixing the conditioned media with DTNB solution at 450 nm of absorbance (DTX880, Beckman Coulter, Indianapolis, IN, USA). The value from DTNB only was substracted as noise ...
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bioRxiv - Bioengineering 2024Quote: ... using C4F10-saturated PBS until the subnatant was clear after which MBs were counted using a Coulter Counter Multisizer 3 (50 µm aperture tube, Beckman Coulter, Mijdrecht, the Netherlands). Next ...
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bioRxiv - Microbiology 2021Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 μL of sterile water ...
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bioRxiv - Cancer Biology 2022Quote: ... was removed using right-side size selection with AMPure XP beads at a 1:1 and 7:1 bead:sample ratio (Beckman Coulter) described previously26 ...
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bioRxiv - Microbiology 2022Quote: ... Pool 1 and pool 2 amplicons were combined and purified with a 1:1 ratio of AMPureXP beads (Beckman Coulter) and eluted in 30 µL of RNAase free water ...
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bioRxiv - Systems Biology 2024Quote: ... After cleanup with AMPure XP Reagent (Beckman Coulter, 1:1 ratio beads:PCR), iPCR amplification was carried out with KAPA HiFi HotStart ReadyMix (Roche) ...
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bioRxiv - Molecular Biology 2023Quote: ... and purified using 1:1 (vol: vol) AMPure XL beads (Beckman Coulter). cDNA prepared as above was end-prepped using NEBNext Companion Module for Oxford Nanopore Technologies Ligation Sequencing (NEB ...
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bioRxiv - Biophysics 2021Quote: ... which were centrifuged at 3000 rpm for 15 minutes at 4°C using a fixed angle centrifuge (Beckman Coulter, Brea, CA, USA). After removal of excess supernatant ...
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bioRxiv - Cancer Biology 2021Quote: Proliferation of IL-32 KO and WT mock cells was assessed by cell counting every day for 4 days using cell coulter (Beckman Coulter Diagnostics). Cell viability was assessed by Trypan blue staining or by annexin/PI flow cytometry and analyzed by FACS Diva software (BD Biosciences).
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bioRxiv - Immunology 2021Quote: ... The pelleted exosomes from above supernatants were further centrifuged at 100,000g (26,450rpm) for 2 h at 4 °C (Beckman Coulter, Optima XE-90) and resuspended in PBS ...
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bioRxiv - Microbiology 2021Quote: ... Samples were centrifuged at 130,000 × g for 2 h at 4°C in a TLA100.3 rotor (Beckman Optima TLX table top centrifuge). Following centrifugation ...
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bioRxiv - Neuroscience 2021Quote: ... The post nuclear supernatant was transferred to an ultracentrifuge tube and centrifuged for 2 h at 245,000 g at 4 °C in a Beckman L90 Ultracentrifuge (Beckman Coulter, Mississauga, Canada) to pellet remaining membrane.
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bioRxiv - Biophysics 2022Quote: ... The tubes were filled with PBS to reach the final volume and samples were ultracentrifuged at 120,000 g for 60 minutes at 4°C (70.1 Ti rotor, k-factor 36, Beckman Coulter, Brea, CA, USA). Finally ...
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bioRxiv - Plant Biology 2020Quote: ... All samples were centrifuged in a single 2 h run with sedimentation at 50,000 g and 4°C using small 13 x 51 mm ultracentrifugation tubes in SW55 Ti rotor (Beckman Coulter, Krefeld, Germany). These centrifugation settings were optimized for polysome and monosome separations ...
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bioRxiv - Cell Biology 2020Quote: ... Vacuoles were separated from free dye by centrifugation (100,000 x g, 25 min, 4°C, SW-60 Ti rotor, Beckman L8-80M Ultracentrifuge) and recovered from the 0%-4% interface ...
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bioRxiv - Biophysics 2021Quote: ... then the supernatant was ultracentrifuged at 100000×g for 20 min at 4 °C using an Optima L-100K ultracentrifuge (Beckman Coulter, USA) equipped with a SW 40 Ti swinging bucket rotor ...