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Citations for Beckman :
1051 - 1100 of 1791 citations for 4 1 tert butoxycarbonyl piperidin 3 yl benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated fibers that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous sucrose gradient (40% ...
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bioRxiv - Microbiology 2022Quote: ... through a syringe needle (0.4-by 20 mm needles) and the cell wall was collected by ultracentrifugation at 90,000 × g for 45 min at 4°C (Beckman Coulter, Inc.). To the pellet ...
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bioRxiv - Bioengineering 2022Quote: ... The final supernatant was collected and EVs were pelleted at 100,000 g at 4°C for four hours in an Optima ultracentrifuge using the Beckman Ti45 rotor (Beckman Coulter, USA). The EVs pellet was washed with PBS and resuspended in 0.22 μm filtered PBS ...
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bioRxiv - Microbiology 2021Quote: ... Cell supernatants containing the pseudotype viral particles were centrifuged at 109,000 g or 35 min at 4 °C using a TLA100.3 rotor with an Optima TLX ultracentrifuge (Beckman Coulter, CA, USA), and the pellet was then lysed for western blotting analysis.
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Adaptive translational pausing is a hallmark of the cellular response to severe environmental stressbioRxiv - Molecular Biology 2020Quote: ... and centrifuged at 4°C at 31,000 rpm for 2 h and 30 min using an SW 32 Ti rotor(Beckman Coulter, 369694). The gradients were fractionated using a Teledyne ISCO Density Gradient Fractionation System and 12 fractions were collected ...
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bioRxiv - Molecular Biology 2020Quote: ... The filtered supernatant was then centrifuged in JA20 tubes at 6000 rpm overnight (O/N) at 4°C (Avanti J-25, Beckman Coulter). The following day the viral pellet was resuspended in 5 ml PBS and centrifuged at 20,000 rpm for 90 mins at 4°C (Optima XL-100K ...
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bioRxiv - Molecular Biology 2020Quote: ... The gradients were centrifuged at 40000 rpm for 2h at 4°C in a SW 60 Ti swinging-bucket rotor (Beckman Coulter). Afterwards ...
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bioRxiv - Cell Biology 2021Quote: ... and centrifuged at 100,000 g at 4°C for 60 min in a swinging rotor (SW40Ti) (Beckman Coulter OptimaTM L–100K). Following centrifugation ...
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Single cell analysis of the cellular heterogeneity and interactions in the injured mouse spinal cordbioRxiv - Neuroscience 2020Quote: ... were added to each sample well and cells were kept at 4°C until analysis on a Cytoflex Flow Cytometer (Beckman Coulter).
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bioRxiv - Cell Biology 2020Quote: ... were pelleted in Beckman Coulter polypropylene open top tubes (110,000 x g for 70 min at 4°C, in Beckman rotor SW28). After washing ...
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bioRxiv - Immunology 2021Quote: ... 4 ng of RNA were retrotranscribed and cDNA was amplified using 13 cycles and purified with AMPure XP beads (Beckman Coulter). Concentration was determined using Qubit 3.0 (Life Technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were grown for 4-6 days and counted using an Incucyte (Essen Biosciences) or a Z2 Coulter Counter (Beckman Coulter). Small molecule dose-response curves were printed using an D300e Digital Dispenser (HP Specialty Printing Systems ...
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bioRxiv - Microbiology 2022Quote: ... and the viral particles were concentrated by ultracentrifugation for 1h at 30,000 rpm at 4°C in an SW40 rotor (Beckman, Fullerton, CA). Viral pellets were resuspended in TNC buffer (10 mM Tris-HCl [pH 7.5] ...
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bioRxiv - Neuroscience 2022Quote: ... dispersed and collected in a single tube and further centrifuged for 4 hours at 21000 rpm in a 20% sucrose gradient (SW55 Beckman rotor). The resulting pellet was suspended in 100 μl PBS and the virus was aliquoted (5-10 μl ...
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bioRxiv - Cancer Biology 2022Quote: ... Crude vesicle pellets were resuspended in 1 ml of exosome-depleted 1X PBS and re-pelleted by centrifugation at 100,000 g for 70 minutes at 4°C (Beckman 45 Ti). Pellets at the bottom of the centrifugation tubes were resuspended in approximately 50ul of 1X PBS ...
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bioRxiv - Systems Biology 2020Quote: ... The resultant supernatant was collected and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55) in 10.4 ml 16×76 mm polycarbonate capped centrifuge tubes (Beckman Coulter 355603), the pellet was washed once in PBS−/− and centrifuged again at 100,000xg for 40 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Reconstituted nucleosome samples were overlaid onto the sucrose gradient and spun at 32000 rpm for 20 h at 4 ° using SW55Ti rotor (Beckman Coulter) in Beckman Optima L80 ...
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bioRxiv - Molecular Biology 2020Quote: ... the lysate was incubated under rotation for 30 min at 4 °C and cleared for 10 min in an MLA-80 rotor (Beckman Coulter) at 11,500 xg and 4 °C ...
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bioRxiv - Molecular Biology 2021Quote: ... Indexing PCR amplicons were pooled (4◻μl of each) and purified using AMPure XP beads (Beckman Coulter, California, United States) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2020Quote: ... cells were centrifuged at 4 ℃ for 5 minutes at 300g and suspended in HBSS and analyzed with a flow cytometer (CytoFLEX, Beckman Coulter). Mean fluorescence intensity was calculated for each group.
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bioRxiv - Systems Biology 2020Quote: ... the 500xg supernatant was first spun at 10,000xg for 10 minutes at 4°C (Beckman Coulter, fixed angle rotor TLA 120.2) in 1 ml 11×34 mm polycarbonate uncapped centrifuge tubes (Beckman Coulter 343778) ...
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bioRxiv - Systems Biology 2020Quote: ... and underwent ultracentrifugation at 100,000xg for 40 minutes at 4°C (rotor MLA-55; Beckman Coulter 355603 10.4 ml capped tubes). Supernatant was discarded from each fraction ...
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bioRxiv - Cell Biology 2020Quote: ... 50 µl of alkaline carbonate treated mitochondria were set aside as input (kept on ice) and 50 µl were centrifuged at 100 000g for 30 min at 4°C in TLA100 rotor (Beckman Coulter). Pellet was gently washed with ice-cold sodium carbonate buffer and resuspended in 20 µl of 1x Laemmli loading buffer ...
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bioRxiv - Immunology 2020Quote: ... during 30min and then transferred into a DuraClone IF T Helper cell tube (CD3, CD4, IL-4, IL-17A, IFNγ) (Beckman Coulter), using the IntraPrep fixation permeabilization kit (Beckman Coulter) ...
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bioRxiv - Molecular Biology 2021Quote: ... The samples were then separated into membrane and matrix fractions by ultracentrifugation at 100 000 g (TLA 110 rotor) for 30 min at 4°C in OptimaTM TLX table-top ultracentrifuge (Beckman-Coulter). The supernatant fraction was transferred to new tube ...
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bioRxiv - Immunology 2021Quote: ... Samples were spun at 76,650 x g for 2 hours at 4° C inside a SW 32i rotor using an Optima L-90K Ultracentrifuge (Beckman Coulter). Purified virus was quantified using a bicinchoninic acid assay (BCA ...
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bioRxiv - Genomics 2021Quote: ... The samples were then centrifuged at 28,600 rpm for 2 h at 4°C (Beckman Optima XE-90; SW32 Ti rotor). After centrifugation ...
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bioRxiv - Immunology 2020Quote: ... Vesicles recovered by ultracentrifugation of filtered supernatant at 150,000 x g for 2 h at 4 °C using a Ti 45 rotor (Beckman Instruments, USA), were washed with phosphate-buffered saline (PBS ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were subsequently incubated with 2.5×103 CFSE-RJ-EVs per cell for 4 hours and analyzed with flow cytometry (CyAn ADP, Beckman Coulter, US). Treatment groups were compared to unstained control as well as CFSE-RJ-EVs without inhibitor ...
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bioRxiv - Cell Biology 2022Quote: Whole bovine milk samples (200 mL) were centrifuged at 3,000 x g for 15 min at 4°C (Allegra X-15R, Beckman Coulter, France) to separate the fat from the skimmed milk ...
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bioRxiv - Cell Biology 2022Quote: ... After ultracentrifugation at 200,000 x g for 5 h at 4°C (SW55Ti rotor in a XL-90 Beckman Coulter centrifuge), fractions containing purified EVs or purified phages were extracted from the top following the scheme in Supp ...
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bioRxiv - Biochemistry 2022Quote: ... Soluble and insoluble fractions were isolated via centrifugation (90,000 x g, 30 min, 4 °C) in an Optima™ MAX-OP (Beckman Coulter) centrifuge using the TLA-100.3 rotor ...
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bioRxiv - Cell Biology 2022Quote: ... The supernatant was recovered and centrifuged at 60,000 rpm for 15 min at 4°C in an Optima TL micro-ultracentrifuges (TLA 100.3 rotor Beckman Coulter, Fullerton, CA) to obtain the Golgi-enriched p100 pellet fraction ...
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bioRxiv - Physiology 2022Quote: ... 40 ml of filtrates were ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 x g for 70 min at 4°C using an Optima L-100 XP Ultracentrifuge equipped with 90 Ti Fixed-Angle Titanium Rotor (Beckman Coulter). The final EVs pellets were suspended in 200 µl of PBS (Lonza) ...
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bioRxiv - Microbiology 2022Quote: ... respectively) by centrifugation at 4000 × rpm for 10 min at 4 °C using an Allegra® X-15R centrifuge (Beckman Coulter) and flash-frozen in liquid nitrogen before RNA isolation ...
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bioRxiv - Neuroscience 2022Quote: ... layers F1/2 were removed before transferring the combined F3/4 layer into a clean 25mL centrifuge tube (Beckman Coulter, #355618) and diluting in GM buffer (at least 1:4 ...
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bioRxiv - Neuroscience 2022Quote: ... The discontinuous sucrose gradient was centrifuged at 242K g for 47 min at 4 °C in a vertical rotor (VTi50 rotor, Beckman Coulter). The bulk GC fraction (GCF ...
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bioRxiv - Microbiology 2022Quote: ... Lysates were then submitted to centrifugation at 100,000 g for 45 min at 4°C in an Ultracentrifuge Beckman XL-90 (Beckman France, Villepinte) equipped with a 70.1T1 rotor ...
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bioRxiv - Biophysics 2024Quote: ... The virions were harvested from the supernatant by centrifugation through 1.5ml of 30% sucrose at 10,000 RPM at 4°C for 2hrs (Beckman L8-70M Ultracentrifuge). The resulting pellet of virions was resuspended in 20ul of RIPA lysis buffer containing protease inhibitors.
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bioRxiv - Neuroscience 2024Quote: ... The same mass of total extracts was taken for a high spin at 154,000 g for 20 minutes in 4 °C using an ultracentrifuge (Beckman, TLX-120K) to obtain the supernatant soluble fraction (S1 ...
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bioRxiv - Neuroscience 2024Quote: ... and centrifuged at ∼14.000 x g max (∼10900 x g average) for 22 min at 4 °C (Beckman Coulter, MAX-XP). After centrifugation ...
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bioRxiv - Molecular Biology 2024Quote: ... for ultracentrifugation at 100,000 rcf for 70 min at 4 °C using a Optima XE-90 ultracentrifuge with a SW 32 Ti rotor (Beckman Coulter). The EV pellet was resuspended in 0.22 μm-filtered sterile PBS and freshly-used or stored at −80 °C until further use ...
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bioRxiv - Microbiology 2024Quote: ... Most of the supernatant was discarded leaving 12x∼200 µL of concentrated broken pieces of genomic fiber that were pooled and layered on top of ultracentrifuge tubes of 4 mL (polypropylene centrifuge tubes, Beckman Coulter) containing a discontinuous cesium chloride gradient (1.4 ...
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bioRxiv - Bioengineering 2023Quote: ... The gradient tube was balanced and subjected to ultracentrifugation at 150,000 × g for 2 h at 4 °C using a swinging-bucket rotor (SW 41 Ti Beckman Coulter, USA).
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bioRxiv - Cancer Biology 2024Quote: ... CRC cells were then cocultured at a 1:1 ratio with BMDCs for 4 hours and analyzed for uptake of CRC DNA by flow cytometry on the CytoFlex S cytometer (Beckman Coulter). Analysis was performed in FlowJo.
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bioRxiv - Bioengineering 2023Quote: ... Samples were ultracentrifuged at 100,000×g for 70 min at 4°C using the Beckman OptimaTM L-80 XP ultracentrifuge (Beckman Coulter, CA). The supernatant was discarded ...
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bioRxiv - Physiology 2023Quote: ... was ultracentrifuged twice in sterile 10.4 mL polycarbonate bottles at 100 000 × g for 70 min at 4°C (Optima L-100 XP Ultracentrifuge, 90 Ti Fixed-Angle Titanium Rotor; Beckman Coulter). The final EV pellet was suspended in 50 µl of PBS without Ca2+ and Mg2+ (Lonza ...
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bioRxiv - Neuroscience 2023Quote: ... Supernatant were loaded on top of a discontinuous 0.75/1.0/2.33 M sucrose density gradient and centrifuge at 242,000 x g for 60 min at 4°C (Beckman SWTi41 swing rotor). The growth cone depleted fraction was collected between 0.75 M and 1.0 M sucrose ...
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bioRxiv - Cell Biology 2023Quote: ... The cilia in the upper phase were sedimented by centrifugation (27,000 g, 4°C, 20 min; Beckman Coulter, Avanti JXN-26) and resuspended in Hepes-Magnesium-EGTA-Potassium (HMEK ...
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bioRxiv - Biochemistry 2023Quote: ... The sample was run on the gradient for 16 hrs at 4 °C using an Optima XE-90 ultracentrifuge (SW40Ti rotor, Beckman-Coulter) at 30,000 rpm ...