Labshake search
Citations for Beckman :
1101 - 1150 of 1934 citations for 4' Trifluoromethoxy 5 trifluoromethyl 1 1' biphenyl 3 carboxylic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Cell pellets were permeabilized for intracellular staining via resuspension in 0.5% Triton X-100 in PBS for 10 min and were subsequently resuspended in a 1:500 dilution of anti-p24-FITC KC57 antibody (Beckman Coulter #6604665) in 1% BSA in PBS for 1 h at room temperature in the dark ...
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bioRxiv - Molecular Biology 2022Quote: ... The percentage of live/dead cells was determined within 1 hour of staining by flow cytometry (CytoFLEX, Beckman Coulter, Indianapolis, IN, USA). 10,000 events were collected per sample ...
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bioRxiv - Cell Biology 2023Quote: ... Supernatants were loaded onto a 1 M sucrose cushion and then pelleted by ultracentrifugation by using a TLA100.3 rotor (Beckman Coulter, cat#349490) for 1 hour at 100,000 rpm at 4°C ...
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bioRxiv - Plant Biology 2023Quote: ... DNA extracts were purified using a bead clean up method with a 2:1 ratio of Ampure XP beads (Beckman Coulter, USA) to DNA elution ...
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bioRxiv - Physiology 2023Quote: ... via centrifugation in a SW41 rotor at 42,000 g at 4 °C for 1 hour in a Beckman Coulter Optima L-100 XP ultracentrifuge using 14 × 89 mm Ultra-Clear Centrifuge Tubes (Beckman Instruments Inc.). Mitochondria were harvested at the interphase between 19 and 40% Percoll ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Then 0.1 μL of library compound (1 mM) dissolved in DMSO was added using pintool integrated with Beckman NX (Beckman Coulter, Brea, CA). The final compound concentration was 20 μM and the final DMSO concentration was 2% ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution) Productively-infected cells were identified based on p24 and CD4 detection as described above ...
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bioRxiv - Microbiology 2024Quote: ... and stained intracellularly using PE or FITC-conjugated mouse anti-p24 mAb (clone KC57; Beckman Coulter, Brea, CA, USA; 1:100 dilution). The percentage of infected cells (p24+ ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells mixed with polybrene and lentivirus were then plated in 1 mL aliquots onto 12 well plates and centrifuged at 931 x g [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were mixed with polybrene and increasing amounts of the lentiviral CP1985 sgRNA library and plated in 1 mL aliquots onto 12 well plates and centrifuged at 931 x g [Allegra® X-15R Centrifuge (Beckman Coulter), rotor SX4750A ...
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bioRxiv - Microbiology 2024Quote: Samples (1 mL) from the mother culture and post treatment culture growth were analysed using a CytoFLEX LX flow cytometer (Beckman Coulter, USA) to compare difference in bacterial contamination ...
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bioRxiv - Molecular Biology 2024Quote: ... 670 µL of lysate was slowly dispensed on top of 330 µL of a 70% sucrose cushion in a 1 mL polycarbonate tube (Beckman Coulter, 343778) and centrifuged in a MLA-130 ultracentrifuge rotor (Beckman Coulter ...
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bioRxiv - Genomics 2024Quote: Cells from frozen stocks were diluted 1:30 into 300 µl of LB in a deep 96-well plate (Beckman Coulter, #267007), covered with a breathable film ...
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bioRxiv - Biophysics 2024Quote: ... Resulting libraries were pooled (with the Mosquito) and purified using AMPure XP magnetic beads at a ratio of 0.8:1 beads:sample volume (Beckman Coulter, Brea, CA, USA). Library yield and quality were determined by TapeStation electrophoresis with an HSD1000 ScreenTape system (Agilent Technologies).
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bioRxiv - Bioengineering 2024Quote: ... a suspension of the microparticles was prepared in double deionized water and sized using a laser diffraction method to measure the size distribution of microparticles (Supplementary Figure 1, Coulter LS230, fitted with the hazardous fluids module, Beckman Coulter, UK) while under agitation to prevent the particles settling ...
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bioRxiv - Genetics 2024Quote: ... A final DNase treatment was performed in 50 μL total volume using 1 μL of Turbo DNase with addition of the buffer incubated for 60 min at 37 °C followed by inactivation with 1 μL of 10% SDS and purification using RNA clean Ampure XP beads (Beckman Coulter, A63987).
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (25000 rpm for 30 min at 4°C using a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Lysates were cleared by ultracentrifugation (40000 rpm for 30 min at 4°C in a Beckman Ti45 rotor). Supernatant was filtered (0.45 μm ...
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bioRxiv - Molecular Biology 2021Quote: ... RNA concentration of the cleared lysate was measured by nanodrop and 500 μg of this RNA was loaded onto the gradient and run for 2.5 hr at 220,000 g and 4°C in an SW41-rotor (Beckman). Gradients were then run at 850 μL/min in a density gradient fractionation system (Teledyne Isco) ...
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bioRxiv - Molecular Biology 2021Quote: ... Gradients were centrifuged at 36000 RPM for 2 hours at 4°C in a SW41 rotor (Beckman Coulter) and then fractions were collected using a gradient collector (Teledyne ISCO gradient station ...
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bioRxiv - Microbiology 2022Quote: ... for 3h at 4°C in a Beckman ultracentrifuge with the SW40ti rotor head (Beckman, Brea, California, USA) using open top polyallomer tubes (Seton Scientific ...
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bioRxiv - Bioengineering 2021Quote: ... The resulting solution was centrifuged at 164,000 × g (30,000 rpm) for 4 h at 20°C (Optima XPN-80 Ultracentrifuge, Beckman) to remove any remaining nanotube aggregates.
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bioRxiv - Bioengineering 2020Quote: ... larger vesicles were depleted by centrifugation for 30 minutes at 10,000xg at 4°C (SW28 rotor, Beckman Coulter). In the final step ...
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bioRxiv - Biochemistry 2020Quote: ... 300mM NaCl (pH 7.0) was performed at 4 ⁰C on an Optima XL-A Analytical Ultracentrifuge (Beckman Coulter). Equilibrium radial distributions at 10,000 and 15,000 RPM of Sfh5 at concentrations of 10 ...
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bioRxiv - Plant Biology 2021Quote: ... The supernatant was transferred into new centrifuge tubes and centrifuged at 40,000g (P40) or 100,000g (P100) for one hour (4°C, TLA100.3, Optima TLX Ultracentrifuge; Beckman Coulter) to pellet EVs and other particles as noted in figure legends ...
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bioRxiv - Biochemistry 2020Quote: ... Beckman CoulterTM at 150.000 g for 120 minutes at 4°C with a TLA-55 Rotor (Beckman CoulterTM) to pellet EVs ...
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bioRxiv - Microbiology 2020Quote: ... Then 4 ml of the adjusted lipoprotein was layered under 8 ml of 0.9% NaCl and centrifuged at 49,000 rpm for 4 hours at 10°C (NVTi 65 rotor; Beckman). The HDL (lower band ...
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bioRxiv - Microbiology 2020Quote: ... Purification was done by ultracentrifugation for 2 h at 154,000xg at 4°C in a SW41 rotor (Beckman) using a two-step sucrose gradient (20%/50% w/v in HNE) ...
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bioRxiv - Biochemistry 2021Quote: ... and separated by ultracentrifugation in a TLA120.2 rotor (Beckman Coulter; 1.5 h at 278,000 x g, 4°C).
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bioRxiv - Cell Biology 2020Quote: ... and cell size and cell density were measured using the Multisizer 4 Coulter Counter (Beckman-Coulter, Mississauga, ON) or collected for whole cell lysis at time points indicated in the figures ...
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bioRxiv - Biophysics 2021Quote: ... Cellular debris was removed by centrifugation at 18k rpm for 60 min at 4 °C (Beckman JA-20). The lysate was incubated with 5mL of washed and packed GST resin (GE Healthcare ...
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bioRxiv - Biophysics 2020Quote: ... and then sonicated and ultracentrifuged (440,000 g, 35 min, 4°C; MLA80 rotor, Beckman Optima™ MAX-TL). The supernatant was applied to a Ni-NTA column (Machery-Nagel ...
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bioRxiv - Cell Biology 2020Quote: ... pellets obtained by ultracentrifugation (100,000 x g in Ti45 Beckman Coulter rotor for 70 min. at 4 ° C) were washed and resuspended in 1.25 ml buffer [0.25 M sucrose ...
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Targeted attenuation of elevated histone marks at SNCA alleviates α-synuclein in Parkinson’s diseasebioRxiv - Neuroscience 2020Quote: ... The samples were then centrifuged at 107,163.6 X g for 2.5 hours at 4°C in an ultracentrifuge (XPN 100, Beckman Coulter). After centrifugation ...
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bioRxiv - Microbiology 2021Quote: ... lysates were placed on top of a 20-50% sucrose gradient and sedimented at 35,000 rpm (218,000x g) for 6 hours at 4°C on SW40Ti rotor (Beckman). For flotation ...
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bioRxiv - Plant Biology 2020Quote: ... and 100 μg/ml cycloheximide) and centrifuged for 1.5 h at 50,000 rpm at 4 °C in a SW55 Ti rotor (Beckman). RNA labeling was performed according to Zoschke et al ...
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bioRxiv - Zoology 2021Quote: ... then held at 4°C) at manufacturer-recommended enzyme concentrations and used AMPure XP beads (Beckman Coulter, #A63881) at a 1.5X ratio to retain only DNA from the digestion ...
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bioRxiv - Genetics 2022Quote: ... were then spun at 19,000rpm/90 minutes/4°C using an ultracentrifuge and a SW28 hanging rota (Beckman). All supernatant was removed and each viral pellet was resuspended with 300 µl of 1% Bovine Serum Albumin (Tocris Bioscience ...
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bioRxiv - Cell Biology 2020Quote: ... Bacteria were spun down at 6000 rpm at 4 °֯C for 30 min in JLA8.1 rotor (Beckman Coulter) in Beckman Avanti J-26S ...
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bioRxiv - Cell Biology 2020Quote: ... and spun at 3,300 (2,647 rcf) rpm at 4 °C for 40 min using JS 5.3 rotor (Beckman Coulter) in Beckman Avanti J-26S ...
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bioRxiv - Cell Biology 2020Quote: ... the cleared supernatant was ultracentrifuged at 47,000 g for 2 hours at 4 °C using the JA25.50 rotor (Beckman). The virus was resuspended overnight in 180 μL D10 medium at 4 °C ...
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bioRxiv - Biochemistry 2020Quote: ... 4 °C) using a TLA-55 rotor in an Optima MAX-XP tabletop ultracentrifuge (Beckman Coulter Life Sciences). EDC (ThermoFischer Scientific ...
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bioRxiv - Biochemistry 2021Quote: ... were pelleted by ultracentrifugation (80,000 rpm, 4°C, 20 min with a TLA 100.1 rotor in a Beckman Optima TLX Ultracentrifuge ...
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bioRxiv - Biochemistry 2021Quote: ... were pelleted by ultracentrifugation (80,000 rpm, 4°C, 20 min with a TLA 100.1 rotor in a Beckman Optima TLX Ultracentrifuge; Beckman Coulter Life Sciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were incubated overnight at 4°C prior to analysis on a Gallios cytometer (Beckman Coulter Life Sciences) using Kaluza 2.1 analysis software (Beckman Coulter).
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bioRxiv - Biochemistry 2021Quote: ... and then centrifuging at 30,000 x g for 25 min at 4 °C in a JA 25.50 rotor (Beckman). GsI-IIC RT and other cellular proteins were then precipitated from the supernatant with 60% saturating ammonium sulfate ...
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bioRxiv - Cell Biology 2021Quote: ... supernatants were spun for 70 minutes at 200,000 × g and 4°C in an XPN-80 ultracentrifuge equipped with a Type 50.4 Ti rotor (Beckman Coulter ...
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bioRxiv - Genetics 2021Quote: ... from pooled wells) were fixed using 4% paraformaldehyde (PFA) and analyzed for GFP fluorescence by CytoFLEX (Beckman-Coulter) to determine the percentage of infected cells ...
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bioRxiv - Cell Biology 2021Quote: ... 4 °C for 2 h in an Optima L-100XP ultracentrifuge (with SW 55 Ti rotor, Beckman Coulter). Fractionation was done manually by collecting every 200 μl volume from the top till the bottom of each centrifuge tube ...